TY  - CONF
AU  - Nikolić, Ljiljana
AU  - Bataveljić, Danijela
AU  - Moldovan, Ivana
AU  - Balind, Snežana
AU  - Todorović, Dajana
AU  - Nedeljković, Miodrag
AU  - Petković, Branka
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4028
AB  - Hibernation is a physiological state that enables certain animal species to survive severe
environmental conditions during the cold periods. In hibernation state the brain activity
is maintained at a very low, but functionally responsive level, indicating that neurons
and glial cells undergo certain physiological modifications. These modifications should
be characterized as a neuroprotective, since the brain of hibernated animals endures extreme
physiological conditions without any damage. In the present study we examined
the electrophysiological responses of neurons and glial cells all acutely isolated from
active and hibernating land snail Helix pomatia by the patch-clamp technique. Hibernating
state lasted six months. Data obtained revealed that the steady-state outward neuronal
current density was significantly lower in hibernation. In addition, the fast Na+
inward current density was significantly reduced in the population of isolated hibernated
neurons, indicating that neuronal activity is suppressed in hibernation. The lower neuronal
activity was supported by the suppressed electrophysiological response of glial
cells. Thus, hibernated glia had significantly lower specific membrane conductance and
reduced inward current density compared to active glia. Particularly important was the
modification of the glial inwardly rectifuing potassium (Kir) channel activity, essential
for the coupling of the function of glial cells with neuronal activity. Thus, in the presence
of BaCl2, Ba2+-sensitive current density mediated by the glial Kir channels was significantly
lower in hibernation. Altogether, our data indicate that overall suppression of
neuronal and glial activity is important natural neuroprotective strategy necessary.
PB  - Belgrade: Serbian Physiological Society
C3  - 3rd Congress of Physiological Sciences of Serbia With International Participation
T1  - Modified Electrophysiological Responses of Neurons and Glial Cells Acutely Isolated From The Hibernating Land Snail
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4028
ER  - 

@conference{
author = "Nikolić, Ljiljana and Bataveljić, Danijela and Moldovan, Ivana and Balind, Snežana and Todorović, Dajana and Nedeljković, Miodrag and Petković, Branka",
year = "2014",
abstract = "Hibernation is a physiological state that enables certain animal species to survive severe
environmental conditions during the cold periods. In hibernation state the brain activity
is maintained at a very low, but functionally responsive level, indicating that neurons
and glial cells undergo certain physiological modifications. These modifications should
be characterized as a neuroprotective, since the brain of hibernated animals endures extreme
physiological conditions without any damage. In the present study we examined
the electrophysiological responses of neurons and glial cells all acutely isolated from
active and hibernating land snail Helix pomatia by the patch-clamp technique. Hibernating
state lasted six months. Data obtained revealed that the steady-state outward neuronal
current density was significantly lower in hibernation. In addition, the fast Na+
inward current density was significantly reduced in the population of isolated hibernated
neurons, indicating that neuronal activity is suppressed in hibernation. The lower neuronal
activity was supported by the suppressed electrophysiological response of glial
cells. Thus, hibernated glia had significantly lower specific membrane conductance and
reduced inward current density compared to active glia. Particularly important was the
modification of the glial inwardly rectifuing potassium (Kir) channel activity, essential
for the coupling of the function of glial cells with neuronal activity. Thus, in the presence
of BaCl2, Ba2+-sensitive current density mediated by the glial Kir channels was significantly
lower in hibernation. Altogether, our data indicate that overall suppression of
neuronal and glial activity is important natural neuroprotective strategy necessary.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "3rd Congress of Physiological Sciences of Serbia With International Participation",
title = "Modified Electrophysiological Responses of Neurons and Glial Cells Acutely Isolated From The Hibernating Land Snail",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4028"
}

Nikolić, L., Bataveljić, D., Moldovan, I., Balind, S., Todorović, D., Nedeljković, M.,& Petković, B.. (2014). Modified Electrophysiological Responses of Neurons and Glial Cells Acutely Isolated From The Hibernating Land Snail. in 3rd Congress of Physiological Sciences of Serbia With International Participation
Belgrade: Serbian Physiological Society., 149.
https://hdl.handle.net/21.15107/rcub_ibiss_4028

Nikolić L, Bataveljić D, Moldovan I, Balind S, Todorović D, Nedeljković M, Petković B. Modified Electrophysiological Responses of Neurons and Glial Cells Acutely Isolated From The Hibernating Land Snail. in 3rd Congress of Physiological Sciences of Serbia With International Participation. 2014;:149.
https://hdl.handle.net/21.15107/rcub_ibiss_4028 .

Nikolić, Ljiljana, Bataveljić, Danijela, Moldovan, Ivana, Balind, Snežana, Todorović, Dajana, Nedeljković, Miodrag, Petković, Branka, "Modified Electrophysiological Responses of Neurons and Glial Cells Acutely Isolated From The Hibernating Land Snail" in 3rd Congress of Physiological Sciences of Serbia With International Participation (2014):149,
https://hdl.handle.net/21.15107/rcub_ibiss_4028 .

TY  - JOUR
AU  - Nikolić, Ljiljana
AU  - Bataveljić, Danijela B
AU  - Anđus, Pavle
AU  - Nedeljković, Miodrag S
AU  - Todorović, Dajana
AU  - Petković, Branka
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/972
UR  - https://jeb.biologists.org/content/216/18/3531
AB  - Compelling evidence supports the use of a moderate static magnetic field (SMF) for therapeutic purposes. In order to provide insight into the mechanisms underlying SMF treatment, it is essential to examine the cellular responses elicited by therapeutically applied SMF, especially in the nervous system. The Na+/K+ pump, by creating and maintaining the gradient of Na+ and K+ ions across the plasma membrane, regulates the physiological properties of neurons. In this study, we examined the expression of the Na+/K+ pump in the isolated brain-subesophageal ganglion complex of the garden snail Helix pomatia, along with the immunoreactivity and current of the Na+/K+ pump in isolated snail neurons after 15 min exposure to a moderate (10 mT) SMF. Western blot and immunofluorescence analysis revealed that 10 mT SMF did not significantly change the expression of the Na+/K+ pump a-subunit in the snail brain and the neuronal cell body. However, our immunofluorescence data showed that SMF treatment induced a significant increase in the Na+/K+ pump a-subunit expression in the neuronal plasma membrane area. This change in Na+/K+ pump expression was reflected in pump activity as demonstrated by the pump current measurements. Whole-cell patch-clamp recordings from isolated snail neurons revealed that Na+/K+ pump current density was significantly increased after the 10 mT SMF treatment. The SMF-induced increase was different in the two groups of control snail neurons, as defined by the pump current level. The results obtained could represent a physiologically important response of neurons to 10 mT SMF comparable in strength to therapeutic applications.
PB  - The Company of Biologists Ltd.
T2  - Journal of Experimental Biology
T1  - Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field
IS  - 18
VL  - 216
DO  - 10.1242/jeb.085332
SP  - 3531
EP  - 3541
ER  - 

@article{
author = "Nikolić, Ljiljana and Bataveljić, Danijela B and Anđus, Pavle and Nedeljković, Miodrag S and Todorović, Dajana and Petković, Branka",
year = "2013",
abstract = "Compelling evidence supports the use of a moderate static magnetic field (SMF) for therapeutic purposes. In order to provide insight into the mechanisms underlying SMF treatment, it is essential to examine the cellular responses elicited by therapeutically applied SMF, especially in the nervous system. The Na+/K+ pump, by creating and maintaining the gradient of Na+ and K+ ions across the plasma membrane, regulates the physiological properties of neurons. In this study, we examined the expression of the Na+/K+ pump in the isolated brain-subesophageal ganglion complex of the garden snail Helix pomatia, along with the immunoreactivity and current of the Na+/K+ pump in isolated snail neurons after 15 min exposure to a moderate (10 mT) SMF. Western blot and immunofluorescence analysis revealed that 10 mT SMF did not significantly change the expression of the Na+/K+ pump a-subunit in the snail brain and the neuronal cell body. However, our immunofluorescence data showed that SMF treatment induced a significant increase in the Na+/K+ pump a-subunit expression in the neuronal plasma membrane area. This change in Na+/K+ pump expression was reflected in pump activity as demonstrated by the pump current measurements. Whole-cell patch-clamp recordings from isolated snail neurons revealed that Na+/K+ pump current density was significantly increased after the 10 mT SMF treatment. The SMF-induced increase was different in the two groups of control snail neurons, as defined by the pump current level. The results obtained could represent a physiologically important response of neurons to 10 mT SMF comparable in strength to therapeutic applications.",
publisher = "The Company of Biologists Ltd.",
journal = "Journal of Experimental Biology",
title = "Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field",
number = "18",
volume = "216",
doi = "10.1242/jeb.085332",
pages = "3531-3541"
}

Nikolić, L., Bataveljić, D. B., Anđus, P., Nedeljković, M. S., Todorović, D.,& Petković, B.. (2013). Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field. in Journal of Experimental Biology
The Company of Biologists Ltd.., 216(18), 3531-3541.
https://doi.org/10.1242/jeb.085332

Nikolić L, Bataveljić DB, Anđus P, Nedeljković MS, Todorović D, Petković B. Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field. in Journal of Experimental Biology. 2013;216(18):3531-3541.
doi:10.1242/jeb.085332 .

Nikolić, Ljiljana, Bataveljić, Danijela B, Anđus, Pavle, Nedeljković, Miodrag S, Todorović, Dajana, Petković, Branka, "Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field" in Journal of Experimental Biology, 216, no. 18 (2013):3531-3541,
https://doi.org/10.1242/jeb.085332 . .

TY  - JOUR
AU  - Nikolić, Ljiljana
AU  - Todorović, Nataša
AU  - Rokić, Milos B
AU  - Kartelija, Gordana S
AU  - Nedeljković, Miodrag S
AU  - Zakrzevska, Joana S
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1406
AB  - The effect of extremely low frequency magnetic fields (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The influence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by (31)P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na(+)/K(+)-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.
T2  - Biological Research
T1  - Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia
IS  - 2
VL  - 43
DO  - 10.4067/S0716-97602010000200012
SP  - 243
EP  - 250
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1406
ER  - 

@article{
author = "Nikolić, Ljiljana and Todorović, Nataša and Rokić, Milos B and Kartelija, Gordana S and Nedeljković, Miodrag S and Zakrzevska, Joana S",
year = "2010",
abstract = "The effect of extremely low frequency magnetic fields (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The influence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by (31)P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na(+)/K(+)-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.",
journal = "Biological Research",
title = "Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia",
number = "2",
volume = "43",
doi = "10.4067/S0716-97602010000200012",
pages = "243-250",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1406"
}

Nikolić, L., Todorović, N., Rokić, M. B., Kartelija, G. S., Nedeljković, M. S.,& Zakrzevska, J. S.. (2010). Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia. in Biological Research, 43(2), 243-250.
https://doi.org/10.4067/S0716-97602010000200012
https://hdl.handle.net/21.15107/rcub_ibiss_1406

Nikolić L, Todorović N, Rokić MB, Kartelija GS, Nedeljković MS, Zakrzevska JS. Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia. in Biological Research. 2010;43(2):243-250.
doi:10.4067/S0716-97602010000200012
https://hdl.handle.net/21.15107/rcub_ibiss_1406 .

Nikolić, Ljiljana, Todorović, Nataša, Rokić, Milos B, Kartelija, Gordana S, Nedeljković, Miodrag S, Zakrzevska, Joana S, "Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia" in Biological Research, 43, no. 2 (2010):243-250,
https://doi.org/10.4067/S0716-97602010000200012 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1406 .