TY  - JOUR
AU  - Nikolić, Ljiljana
AU  - Todorović, Nataša
AU  - Zakrzewska, Joanna S
AU  - Stanić, Marina B
AU  - Rauš Balind, Snežana
AU  - Kalauzi, Aleksandar
AU  - Petković, Branka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1168
UR  - https://link.springer.com/article/10.1007/s00359-012-0727-0
AB  - The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.
PB  - Springer
T2  - Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
T1  - Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field
IS  - 7
VL  - 198
DO  - 10.1007/s00359-012-0727-0
SP  - 525
EP  - 540
ER  - 

@article{
author = "Nikolić, Ljiljana and Todorović, Nataša and Zakrzewska, Joanna S and Stanić, Marina B and Rauš Balind, Snežana and Kalauzi, Aleksandar and Petković, Branka",
year = "2012",
abstract = "The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.",
publisher = "Springer",
journal = "Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology",
title = "Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field",
number = "7",
volume = "198",
doi = "10.1007/s00359-012-0727-0",
pages = "525-540"
}

Nikolić, L., Todorović, N., Zakrzewska, J. S., Stanić, M. B., Rauš Balind, S., Kalauzi, A.,& Petković, B.. (2012). Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
Springer., 198(7), 525-540.
https://doi.org/10.1007/s00359-012-0727-0

Nikolić L, Todorović N, Zakrzewska JS, Stanić MB, Rauš Balind S, Kalauzi A, Petković B. Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology. 2012;198(7):525-540.
doi:10.1007/s00359-012-0727-0 .

Nikolić, Ljiljana, Todorović, Nataša, Zakrzewska, Joanna S, Stanić, Marina B, Rauš Balind, Snežana, Kalauzi, Aleksandar, Petković, Branka, "Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field" in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology, 198, no. 7 (2012):525-540,
https://doi.org/10.1007/s00359-012-0727-0 . .

TY  - JOUR
AU  - Nikolić, Ljiljana
AU  - Todorović, Nataša
AU  - Rokić, Milos B
AU  - Kartelija, Gordana S
AU  - Nedeljković, Miodrag S
AU  - Zakrzevska, Joana S
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1406
AB  - The effect of extremely low frequency magnetic fields (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The influence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by (31)P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na(+)/K(+)-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.
T2  - Biological Research
T1  - Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia
IS  - 2
VL  - 43
DO  - 10.4067/S0716-97602010000200012
SP  - 243
EP  - 250
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1406
ER  - 

@article{
author = "Nikolić, Ljiljana and Todorović, Nataša and Rokić, Milos B and Kartelija, Gordana S and Nedeljković, Miodrag S and Zakrzevska, Joana S",
year = "2010",
abstract = "The effect of extremely low frequency magnetic fields (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The influence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by (31)P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na(+)/K(+)-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.",
journal = "Biological Research",
title = "Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia",
number = "2",
volume = "43",
doi = "10.4067/S0716-97602010000200012",
pages = "243-250",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1406"
}

Nikolić, L., Todorović, N., Rokić, M. B., Kartelija, G. S., Nedeljković, M. S.,& Zakrzevska, J. S.. (2010). Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia. in Biological Research, 43(2), 243-250.
https://doi.org/10.4067/S0716-97602010000200012
https://hdl.handle.net/21.15107/rcub_ibiss_1406

Nikolić L, Todorović N, Rokić MB, Kartelija GS, Nedeljković MS, Zakrzevska JS. Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia. in Biological Research. 2010;43(2):243-250.
doi:10.4067/S0716-97602010000200012
https://hdl.handle.net/21.15107/rcub_ibiss_1406 .

Nikolić, Ljiljana, Todorović, Nataša, Rokić, Milos B, Kartelija, Gordana S, Nedeljković, Miodrag S, Zakrzevska, Joana S, "Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia" in Biological Research, 43, no. 2 (2010):243-250,
https://doi.org/10.4067/S0716-97602010000200012 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1406 .