TY  - JOUR
AU  - Macut, Djuro
AU  - Bozic-Antic, Ivana
AU  - Brkljačić, Jelena
AU  - Topalovic, Vladanka
AU  - Macut, Jelica Bjekic
AU  - Panidis, Dimitrios
AU  - Kotlica, Biljana Kastratovic
AU  - Papadakis, Efstathios
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2069
AB  - OBJECTIVE: Most women with PCOS have increased adrenal androgen
   production, enhanced peripheral metabolism of cortisol and elevation in
   urinary excretion of its metabolites. Increased cortisol clearance in
   PCOS is followed by a compensatory overdrive of the
   hypothalamic-pituitary-adrenocortical (HPA) axis. We hypothesized that
   oral contraceptives containing ethinylestradiol and drospirenone
   (EE-DRSP) could modulate glucocorticoid receptor (GR) expression and
   function and thus affect HPA axis activity in PCOS patients. DESIGN: We
   analyzed 12 women with PCOS (age 24.17 +/- 4.88 years; body mass index
   22.05 +/- 3.97 kg/m(2)) treated for 12 months with EE-DRSP and 20 BMI
   matched controls. In all subjects testosterone, dehydroepiandrosterone
   sulfate (DHEAS), sex hormone binding globulin (SHBG), cortisol (basal
   and after dexamethasone), concentrations of GR protein, phospo-GR211
   protein, number of GR per cell (B-max) and its equilibrium dissociation
   constant (K-D) were measured. RESULTS: Before treatment, increased
   concentrations of testosterone and DHEAS (p<0.001, respectively),
   unaltered basal cortisol and an increased sensitivity (p<0.05) of the
   HPA axis to dexamethasone were observed in PCOS women in comparison to
   controls. After treatment, testosterone (p<0.01), DHEAS (p<0.05) and
   cortisol suppression after dexamethasone (p<0.01) were decreased in PCOS
   women. There were no changes in GR protein concentration, GR
   phosphorylation nor in the receptor functional parameters B-max and K-D
   in women with PCOS before and after the therapy, and in comparison to
   controls. CONCLUSIONS: Prolonged treatment with EE-DRSP in PCOS women
   decreased serum androgens and increased cortisol in the presence of
   decreased sensitivity of the HPA axis and did not exert changes in GR
   expression and function.
T2  - Hormones-International Journal of Endocrinology and Metabolism
T1  - The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome
IS  - 1
VL  - 14
SP  - 109
EP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2069
ER  - 

@article{
author = "Macut, Djuro and Bozic-Antic, Ivana and Brkljačić, Jelena and Topalovic, Vladanka and Macut, Jelica Bjekic and Panidis, Dimitrios and Kotlica, Biljana Kastratovic and Papadakis, Efstathios and Matić, Gordana and Vojnović-Milutinović, Danijela",
year = "2015",
abstract = "OBJECTIVE: Most women with PCOS have increased adrenal androgen
   production, enhanced peripheral metabolism of cortisol and elevation in
   urinary excretion of its metabolites. Increased cortisol clearance in
   PCOS is followed by a compensatory overdrive of the
   hypothalamic-pituitary-adrenocortical (HPA) axis. We hypothesized that
   oral contraceptives containing ethinylestradiol and drospirenone
   (EE-DRSP) could modulate glucocorticoid receptor (GR) expression and
   function and thus affect HPA axis activity in PCOS patients. DESIGN: We
   analyzed 12 women with PCOS (age 24.17 +/- 4.88 years; body mass index
   22.05 +/- 3.97 kg/m(2)) treated for 12 months with EE-DRSP and 20 BMI
   matched controls. In all subjects testosterone, dehydroepiandrosterone
   sulfate (DHEAS), sex hormone binding globulin (SHBG), cortisol (basal
   and after dexamethasone), concentrations of GR protein, phospo-GR211
   protein, number of GR per cell (B-max) and its equilibrium dissociation
   constant (K-D) were measured. RESULTS: Before treatment, increased
   concentrations of testosterone and DHEAS (p<0.001, respectively),
   unaltered basal cortisol and an increased sensitivity (p<0.05) of the
   HPA axis to dexamethasone were observed in PCOS women in comparison to
   controls. After treatment, testosterone (p<0.01), DHEAS (p<0.05) and
   cortisol suppression after dexamethasone (p<0.01) were decreased in PCOS
   women. There were no changes in GR protein concentration, GR
   phosphorylation nor in the receptor functional parameters B-max and K-D
   in women with PCOS before and after the therapy, and in comparison to
   controls. CONCLUSIONS: Prolonged treatment with EE-DRSP in PCOS women
   decreased serum androgens and increased cortisol in the presence of
   decreased sensitivity of the HPA axis and did not exert changes in GR
   expression and function.",
journal = "Hormones-International Journal of Endocrinology and Metabolism",
title = "The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome",
number = "1",
volume = "14",
pages = "109-117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2069"
}

Macut, D., Bozic-Antic, I., Brkljačić, J., Topalovic, V., Macut, J. B., Panidis, D., Kotlica, B. K., Papadakis, E., Matić, G.,& Vojnović-Milutinović, D.. (2015). The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome. in Hormones-International Journal of Endocrinology and Metabolism, 14(1), 109-117.
https://hdl.handle.net/21.15107/rcub_ibiss_2069

Macut D, Bozic-Antic I, Brkljačić J, Topalovic V, Macut JB, Panidis D, Kotlica BK, Papadakis E, Matić G, Vojnović-Milutinović D. The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome. in Hormones-International Journal of Endocrinology and Metabolism. 2015;14(1):109-117.
https://hdl.handle.net/21.15107/rcub_ibiss_2069 .

Macut, Djuro, Bozic-Antic, Ivana, Brkljačić, Jelena, Topalovic, Vladanka, Macut, Jelica Bjekic, Panidis, Dimitrios, Kotlica, Biljana Kastratovic, Papadakis, Efstathios, Matić, Gordana, Vojnović-Milutinović, Danijela, "The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome" in Hormones-International Journal of Endocrinology and Metabolism, 14, no. 1 (2015):109-117,
https://hdl.handle.net/21.15107/rcub_ibiss_2069 .