TY  - JOUR
AU  - Stanojević, Maja
AU  - Li, Kun
AU  - Stamenković, Gorana
AU  - Ilić, Bojan
AU  - Paunović, Milan
AU  - Pešić, Branislav
AU  - Đurić Maslovara, Ivana
AU  - Šiljić, Marina
AU  - Ćirković, Valentina
AU  - Zhang, Yongzhen
PY  - 2020
UR  - https://www.mdpi.com/1999-4915/12/9/975
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32887342
UR  - https://radar.ibiss.bg.ac.rs/123456789/3880
AB  - Hematophagous arthropods are important vectors for zoonotic pathogens. To date, a huge number of viruses have been identified in these arthropods, with a considerable proportion of them being human pathogens. However, the viromes of hematophagous arthropods are still largely unresearched. In this study, a number of arthropods were collected from Belgrade, Serbia including mosquitoes, ticks and bedbugs. The viromes of these arthropods were identified and characterized using Illumina MiSeq sequencing. In total, 21 viruses belonging to 11 families were characterized, with 11 of them representing novel species. These results may contribute to our knowledge of RNA viruses in arthropods and the discovery of novel human pathogens.
PB  - NLM (Medline)
T2  - Viruses
T1  - Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia
IS  - 9
VL  - 12
DO  - 10.3390/v12090975
SP  - 975
ER  - 

@article{
author = "Stanojević, Maja and Li, Kun and Stamenković, Gorana and Ilić, Bojan and Paunović, Milan and Pešić, Branislav and Đurić Maslovara, Ivana and Šiljić, Marina and Ćirković, Valentina and Zhang, Yongzhen",
year = "2020",
abstract = "Hematophagous arthropods are important vectors for zoonotic pathogens. To date, a huge number of viruses have been identified in these arthropods, with a considerable proportion of them being human pathogens. However, the viromes of hematophagous arthropods are still largely unresearched. In this study, a number of arthropods were collected from Belgrade, Serbia including mosquitoes, ticks and bedbugs. The viromes of these arthropods were identified and characterized using Illumina MiSeq sequencing. In total, 21 viruses belonging to 11 families were characterized, with 11 of them representing novel species. These results may contribute to our knowledge of RNA viruses in arthropods and the discovery of novel human pathogens.",
publisher = "NLM (Medline)",
journal = "Viruses",
title = "Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia",
number = "9",
volume = "12",
doi = "10.3390/v12090975",
pages = "975"
}

Stanojević, M., Li, K., Stamenković, G., Ilić, B., Paunović, M., Pešić, B., Đurić Maslovara, I., Šiljić, M., Ćirković, V.,& Zhang, Y.. (2020). Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia. in Viruses
NLM (Medline)., 12(9), 975.
https://doi.org/10.3390/v12090975

Stanojević M, Li K, Stamenković G, Ilić B, Paunović M, Pešić B, Đurić Maslovara I, Šiljić M, Ćirković V, Zhang Y. Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia. in Viruses. 2020;12(9):975.
doi:10.3390/v12090975 .

Stanojević, Maja, Li, Kun, Stamenković, Gorana, Ilić, Bojan, Paunović, Milan, Pešić, Branislav, Đurić Maslovara, Ivana, Šiljić, Marina, Ćirković, Valentina, Zhang, Yongzhen, "Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia" in Viruses, 12, no. 9 (2020):975,
https://doi.org/10.3390/v12090975 . .

TY  - JOUR
AU  - Ćirković, Valentina
AU  - Stamenković, Gorana
AU  - Jovanović, Jelena
AU  - Šiljić, Marina
AU  - Paunović, Milan
AU  - Stanojević, Maja
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2547
AB  - Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.
T2  - Tropical Biomedicine
T1  - Failure to detect viral RNA in bat samples collected in the Balkan region
IS  - 4
VL  - 33
SP  - 780
EP  - 785
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2547
ER  - 

@article{
author = "Ćirković, Valentina and Stamenković, Gorana and Jovanović, Jelena and Šiljić, Marina and Paunović, Milan and Stanojević, Maja",
year = "2016",
abstract = "Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.",
journal = "Tropical Biomedicine",
title = "Failure to detect viral RNA in bat samples collected in the Balkan region",
number = "4",
volume = "33",
pages = "780-785",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2547"
}

Ćirković, V., Stamenković, G., Jovanović, J., Šiljić, M., Paunović, M.,& Stanojević, M.. (2016). Failure to detect viral RNA in bat samples collected in the Balkan region. in Tropical Biomedicine, 33(4), 780-785.
https://hdl.handle.net/21.15107/rcub_ibiss_2547

Ćirković V, Stamenković G, Jovanović J, Šiljić M, Paunović M, Stanojević M. Failure to detect viral RNA in bat samples collected in the Balkan region. in Tropical Biomedicine. 2016;33(4):780-785.
https://hdl.handle.net/21.15107/rcub_ibiss_2547 .

Ćirković, Valentina, Stamenković, Gorana, Jovanović, Jelena, Šiljić, Marina, Paunović, Milan, Stanojević, Maja, "Failure to detect viral RNA in bat samples collected in the Balkan region" in Tropical Biomedicine, 33, no. 4 (2016):780-785,
https://hdl.handle.net/21.15107/rcub_ibiss_2547 .

TY  - JOUR
AU  - Tovilović-Kovačević, Gordana
AU  - Ristić, Biljana
AU  - Šiljić, Marina
AU  - Nikolić, Valentina
AU  - Kravić-Stevović, Tamara
AU  - Dulović, Marija
AU  - Milenković, Marina
AU  - Knežević, Aleksandra
AU  - Bošnjak, Mihajlo
AU  - Bumbaširević, Vladimir
AU  - Stanojević, Maja
AU  - Trajković, Vladimir
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6345
AB  - We investigated the role of autophagy, a stress-inducible lysosomal self-digestion of cellular components, in modulation of herpes simplex virus type 1 (HSV-1)-triggered death of U251 human glioma cells. HSV-1 caused apoptotic death in U251 cells, characterized by phosphatidylserine externalization, caspase activation and DNA fragmentation. HSV-1-induced apoptosis was associated with the induction of autophagic response, as confirmed by the conversion of cytosolic LC3-I to autophagosome-associated LC3-II, increase in intracellular acidification, presence of autophagic vesicles, and increase in proteolysis of the selective autophagic target p62. HSV-1-triggered autophagy was not associated with the significant increase in the expression of proautophagic protein beclin-1 or downregulation of the major autophagy suppressor mammalian target of rapamycin (mTOR). Moreover, the phosphorylation of mTOR and its direct substrate p70 S6 kinase was augmented by HSV-1 infection, while the mTOR stimulator Akt and inhibitor AMPK-activated protein kinase (AMPK) were accordingly activated and suppressed, respectively. An shRNA-mediated knockdown of the autophagy-essential LC3b, as well as pharmacological inhibition of autophagy with bafilomycin A1 or 3-methyladenine, markedly accelerated apoptotic changes and ensuing cell death in HSV-1-infected glioma cells. These data indicate that AMPK/Akt/mTOR-independent autophagy could prolong survival of HSV-1-infected U251 glioma cells by counteracting the coinciding apoptotic response.
PB  - Elsevier Masson SAS
T2  - Microbes and Infection
T1  - mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells
IS  - 8-9
VL  - 15
DO  - 10.1016/j.micinf.2013.04.012
SP  - 615
EP  - 624
ER  - 

@article{
author = "Tovilović-Kovačević, Gordana and Ristić, Biljana and Šiljić, Marina and Nikolić, Valentina and Kravić-Stevović, Tamara and Dulović, Marija and Milenković, Marina and Knežević, Aleksandra and Bošnjak, Mihajlo and Bumbaširević, Vladimir and Stanojević, Maja and Trajković, Vladimir",
year = "2013",
abstract = "We investigated the role of autophagy, a stress-inducible lysosomal self-digestion of cellular components, in modulation of herpes simplex virus type 1 (HSV-1)-triggered death of U251 human glioma cells. HSV-1 caused apoptotic death in U251 cells, characterized by phosphatidylserine externalization, caspase activation and DNA fragmentation. HSV-1-induced apoptosis was associated with the induction of autophagic response, as confirmed by the conversion of cytosolic LC3-I to autophagosome-associated LC3-II, increase in intracellular acidification, presence of autophagic vesicles, and increase in proteolysis of the selective autophagic target p62. HSV-1-triggered autophagy was not associated with the significant increase in the expression of proautophagic protein beclin-1 or downregulation of the major autophagy suppressor mammalian target of rapamycin (mTOR). Moreover, the phosphorylation of mTOR and its direct substrate p70 S6 kinase was augmented by HSV-1 infection, while the mTOR stimulator Akt and inhibitor AMPK-activated protein kinase (AMPK) were accordingly activated and suppressed, respectively. An shRNA-mediated knockdown of the autophagy-essential LC3b, as well as pharmacological inhibition of autophagy with bafilomycin A1 or 3-methyladenine, markedly accelerated apoptotic changes and ensuing cell death in HSV-1-infected glioma cells. These data indicate that AMPK/Akt/mTOR-independent autophagy could prolong survival of HSV-1-infected U251 glioma cells by counteracting the coinciding apoptotic response.",
publisher = "Elsevier Masson SAS",
journal = "Microbes and Infection",
title = "mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells",
number = "8-9",
volume = "15",
doi = "10.1016/j.micinf.2013.04.012",
pages = "615-624"
}

Tovilović-Kovačević, G., Ristić, B., Šiljić, M., Nikolić, V., Kravić-Stevović, T., Dulović, M., Milenković, M., Knežević, A., Bošnjak, M., Bumbaširević, V., Stanojević, M.,& Trajković, V.. (2013). mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells. in Microbes and Infection
Elsevier Masson SAS., 15(8-9), 615-624.
https://doi.org/10.1016/j.micinf.2013.04.012

Tovilović-Kovačević G, Ristić B, Šiljić M, Nikolić V, Kravić-Stevović T, Dulović M, Milenković M, Knežević A, Bošnjak M, Bumbaširević V, Stanojević M, Trajković V. mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells. in Microbes and Infection. 2013;15(8-9):615-624.
doi:10.1016/j.micinf.2013.04.012 .

Tovilović-Kovačević, Gordana, Ristić, Biljana, Šiljić, Marina, Nikolić, Valentina, Kravić-Stevović, Tamara, Dulović, Marija, Milenković, Marina, Knežević, Aleksandra, Bošnjak, Mihajlo, Bumbaširević, Vladimir, Stanojević, Maja, Trajković, Vladimir, "mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells" in Microbes and Infection, 15, no. 8-9 (2013):615-624,
https://doi.org/10.1016/j.micinf.2013.04.012 . .