TY  - CONF
AU  - Podgorac, Jelena
AU  - Petković, Branka
PY  - 2023
UR  - https://www.rad-conference.org/RAD_2023-Book_of_Abstracts.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6283
AB  - Introduction: Treatment of epilepsy always has an individual and unique path. Despite effective 
classical and novel antiepileptic drugs (AEDs), monotherapy is limited and insufficient in some cases, such as 
refractory epilepsy. Pregnant women with epilepsy are a population that requires a special approach. Protocol 
guidelines and experience dictate the rational use of AEDs during pregnancy, which means that monotherapy 
and the lowest therapeutic (curable) dose should be used. Epilepsy as a multifactorial condition involving 
different categories of epileptic disorders sometimes requires treatment with highly potent therapeutic drugs, 
such as valproic acid (VPA), which is known to be teratogenic. In some cases, not only can it not be 
substituted, but it must also be combined with another drug. The combination of VPA and levetiracetam 
(LEV), at a dosage of 1:1, achieves additive efficacy with no evidence of toxicity.
Aim: Thus, the aim of the present study was to investigate the sensorimotor development of mice whose 
mothers were treated with a combination of VPA and LEV (1:1 ratio) during breeding and gestation.
Method: Adult, 8-week-old female NMRI mice were used in this study. Two groups of animals were 
formed: one group (8 females) treated with a combination of LEV at a dose of 211 mg/kg/day (LEV-211) and 
VPA at a dose of 200 mg/kg/day (VPA-200), and a control group (8 females) receiving an equivalent amount 
of saline. The doses administered correspond to human doses of 1000 mg/day for both antiepileptic drugs. 
All animals were treated subcutaneously into the loose skin on the back of the neck twice daily. Treatment 
was initiated at the mating of males and females and continued throughout the entire period of breeding and 
gestation. After the females gave birth, each of them was housed separately with her litter. Offspring were 
separated by sex on postnatal day (PND) 21. Sensorimotor system performance and conditional learning 
were assessed on PND25 and PND32 in the hot plate test (HPT).
Results: Female and male offspring treated prenatally with a combination of AEDs (LEV-211 + VPA 200) showed a significantly faster response than the control group on PND32, whereas female offspring also 
showed a faster response than the control group on the first day of testing, PND25.
Conclusion: Considering the results of the HPT and the different sensorimotor responses in offspring 
prenatally treated with antiepileptic drug combinations in animal models, it would be necessary for the 
human practice to continuously monitor (until the end of adolescence) the children prenatally exposed to this 
antiepileptic drug therapy.
PB  - Niš, Serbia: RAD Centre
C3  - Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro
T1  - Prenatal exposure to an antiepileptic combination  (levetiracetam and valproic acid) throughout gestation  and postnatal sensorimotor development in mice
DO  - 10.21175/rad.abstr.book.2023.6.8
SP  - 29
ER  - 

@conference{
author = "Podgorac, Jelena and Petković, Branka",
year = "2023",
abstract = "Introduction: Treatment of epilepsy always has an individual and unique path. Despite effective 
classical and novel antiepileptic drugs (AEDs), monotherapy is limited and insufficient in some cases, such as 
refractory epilepsy. Pregnant women with epilepsy are a population that requires a special approach. Protocol 
guidelines and experience dictate the rational use of AEDs during pregnancy, which means that monotherapy 
and the lowest therapeutic (curable) dose should be used. Epilepsy as a multifactorial condition involving 
different categories of epileptic disorders sometimes requires treatment with highly potent therapeutic drugs, 
such as valproic acid (VPA), which is known to be teratogenic. In some cases, not only can it not be 
substituted, but it must also be combined with another drug. The combination of VPA and levetiracetam 
(LEV), at a dosage of 1:1, achieves additive efficacy with no evidence of toxicity.
Aim: Thus, the aim of the present study was to investigate the sensorimotor development of mice whose 
mothers were treated with a combination of VPA and LEV (1:1 ratio) during breeding and gestation.
Method: Adult, 8-week-old female NMRI mice were used in this study. Two groups of animals were 
formed: one group (8 females) treated with a combination of LEV at a dose of 211 mg/kg/day (LEV-211) and 
VPA at a dose of 200 mg/kg/day (VPA-200), and a control group (8 females) receiving an equivalent amount 
of saline. The doses administered correspond to human doses of 1000 mg/day for both antiepileptic drugs. 
All animals were treated subcutaneously into the loose skin on the back of the neck twice daily. Treatment 
was initiated at the mating of males and females and continued throughout the entire period of breeding and 
gestation. After the females gave birth, each of them was housed separately with her litter. Offspring were 
separated by sex on postnatal day (PND) 21. Sensorimotor system performance and conditional learning 
were assessed on PND25 and PND32 in the hot plate test (HPT).
Results: Female and male offspring treated prenatally with a combination of AEDs (LEV-211 + VPA 200) showed a significantly faster response than the control group on PND32, whereas female offspring also 
showed a faster response than the control group on the first day of testing, PND25.
Conclusion: Considering the results of the HPT and the different sensorimotor responses in offspring 
prenatally treated with antiepileptic drug combinations in animal models, it would be necessary for the 
human practice to continuously monitor (until the end of adolescence) the children prenatally exposed to this 
antiepileptic drug therapy.",
publisher = "Niš, Serbia: RAD Centre",
journal = "Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro",
title = "Prenatal exposure to an antiepileptic combination  (levetiracetam and valproic acid) throughout gestation  and postnatal sensorimotor development in mice",
doi = "10.21175/rad.abstr.book.2023.6.8",
pages = "29"
}

Podgorac, J.,& Petković, B.. (2023). Prenatal exposure to an antiepileptic combination  (levetiracetam and valproic acid) throughout gestation  and postnatal sensorimotor development in mice. in Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro
Niš, Serbia: RAD Centre., 29.
https://doi.org/10.21175/rad.abstr.book.2023.6.8

Podgorac J, Petković B. Prenatal exposure to an antiepileptic combination  (levetiracetam and valproic acid) throughout gestation  and postnatal sensorimotor development in mice. in Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro. 2023;:29.
doi:10.21175/rad.abstr.book.2023.6.8 .

Podgorac, Jelena, Petković, Branka, "Prenatal exposure to an antiepileptic combination  (levetiracetam and valproic acid) throughout gestation  and postnatal sensorimotor development in mice" in Book of Abstracts: Eleventh International Conference on Radiation, Natural Sciences, Medicine, Engineering, Technology and Ecology: RAD 2023; 2023 Jun 19-23; Herceg Novi, Montenegro (2023):29,
https://doi.org/10.21175/rad.abstr.book.2023.6.8 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Sekulić, Slobodan
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Pešić, Vesna
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9557044
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5169
AB  - Clinical findings show that the use of valproic acid (VPA) during pregnancy increases the risk of birth defects and autism spectrum disorder in offspring. Although there is a consensus that monitoring of potential long-term outcomes of VPA exposure is needed, especially in undiagnosed individuals, preclinical studies addressing this issue are rare. The present study examined the effects of continuous intrauterine exposure to a wide dose range of VPA (50, 100, 200, and 400 mg/kg/day) on the physical and behavioral response in peripubertal mice as a rodent model of adolescence. Body weight and the hot plate test [on postnatal days (PND) 25 and 32], the elevated plus-maze test (on PND35), and the open field test (on PND40) served to examine physical growth, the supraspinal reflex response to a painful thermal stimulus and conditional learning, anxiety-like/risk-assessment behavior, as well as novelty-induced psychomotor activity, respectively. VPA exposure produced the following responses: (i) a negative effect on body weight, except for the dose of 100 mg/kg/day in both sexes; (ii) an increase in the percentage of animals that responded to the thermal stimulus above the defined cut-off time interval and the response latency in both sexes; (iii) dose-specific changes within sexes in behavior provoked by a novel anxiogenic environment, i.e., in females less anxiety-like/risk-assessment behavior in response to the lowest exposure dose, and in males more pronounced anxiety-like/risk-assessment behavior after exposure to the highest dose and 100 mg/kg/day; (iv) dose-specific changes within sexes in novelty-induced psychomotor activity, i.e., in females a decrease in stereotypy-like activity along with an increase in rearing, and in males a decrease in stereotypy-like activity only. These findings show that continuous intrauterine exposure to VPA produces maladaptive functioning in different behavioral domains in adolescence and that the consequences are delicate to assess as they are dose-related within sexes.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Behavioral Neuroscience
T1  - The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes
VL  - 16
DO  - 10.3389/fnbeh.2022.982811
SP  - 982811
ER  - 

@article{
author = "Podgorac, Jelena and Sekulić, Slobodan and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Pešić, Vesna",
year = "2022",
abstract = "Clinical findings show that the use of valproic acid (VPA) during pregnancy increases the risk of birth defects and autism spectrum disorder in offspring. Although there is a consensus that monitoring of potential long-term outcomes of VPA exposure is needed, especially in undiagnosed individuals, preclinical studies addressing this issue are rare. The present study examined the effects of continuous intrauterine exposure to a wide dose range of VPA (50, 100, 200, and 400 mg/kg/day) on the physical and behavioral response in peripubertal mice as a rodent model of adolescence. Body weight and the hot plate test [on postnatal days (PND) 25 and 32], the elevated plus-maze test (on PND35), and the open field test (on PND40) served to examine physical growth, the supraspinal reflex response to a painful thermal stimulus and conditional learning, anxiety-like/risk-assessment behavior, as well as novelty-induced psychomotor activity, respectively. VPA exposure produced the following responses: (i) a negative effect on body weight, except for the dose of 100 mg/kg/day in both sexes; (ii) an increase in the percentage of animals that responded to the thermal stimulus above the defined cut-off time interval and the response latency in both sexes; (iii) dose-specific changes within sexes in behavior provoked by a novel anxiogenic environment, i.e., in females less anxiety-like/risk-assessment behavior in response to the lowest exposure dose, and in males more pronounced anxiety-like/risk-assessment behavior after exposure to the highest dose and 100 mg/kg/day; (iv) dose-specific changes within sexes in novelty-induced psychomotor activity, i.e., in females a decrease in stereotypy-like activity along with an increase in rearing, and in males a decrease in stereotypy-like activity only. These findings show that continuous intrauterine exposure to VPA produces maladaptive functioning in different behavioral domains in adolescence and that the consequences are delicate to assess as they are dose-related within sexes.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Behavioral Neuroscience",
title = "The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes",
volume = "16",
doi = "10.3389/fnbeh.2022.982811",
pages = "982811"
}

Podgorac, J., Sekulić, S., Petković, B., Stojadinović, G., Martać, L.,& Pešić, V.. (2022). The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes. in Frontiers in Behavioral Neuroscience
Lausanne: Frontiers Media S.A.., 16, 982811.
https://doi.org/10.3389/fnbeh.2022.982811

Podgorac J, Sekulić S, Petković B, Stojadinović G, Martać L, Pešić V. The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes. in Frontiers in Behavioral Neuroscience. 2022;16:982811.
doi:10.3389/fnbeh.2022.982811 .

Podgorac, Jelena, Sekulić, Slobodan, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Pešić, Vesna, "The influence of continuous prenatal exposure to valproic acid on physical, nociceptive, emotional and psychomotor responses during adolescence in mice: Dose-related effects within sexes" in Frontiers in Behavioral Neuroscience, 16 (2022):982811,
https://doi.org/10.3389/fnbeh.2022.982811 . .

TY  - JOUR
AU  - Sekulić, Slobodan
AU  - Jakovljević, Branislava
AU  - Korovljev, Darinka
AU  - Simić, Svetlana
AU  - Čapo, Ivan
AU  - Podgorac, Jelena
AU  - Martać, Ljiljana
AU  - Kesić, Srđan
AU  - Rakić, Srđan
AU  - Petković, Branka
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2021.810391/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4787
AB  - Polyhydramnios is a condition related to an excessive accumulation of amniotic fluid
in the third trimester of pregnancy and it can be acute and chronic depending on
the duration. Published data suggest that during muscle development, in the stage
of late histochemical differentiation decreased mechanical loading cause decreased
expression of myosin heavy chain (MHC) type 1 leading to slow-to-fast transition. In the
case of chronic polyhydramnios, histochemical muscle differentiation could be affected
as a consequence of permanent decreased physical loading. Most affected would
be muscles which are the most active i.e., spine extensor muscles and muscles of
legs. Long-lasting decreased mechanical loading on muscle should cause decreased
expression of MHC type 1 leading to slow-to-fast transition, decreased number of
muscle fiber type I especially in extensor muscles of spine and legs. Additionally,
because MHC type 1 is present in all skeletal muscles it could lead to various degrees
of hypotrophy depending on constituting a percentage of MHC type 1 in affected
muscles. These changes in the case of preexisting muscle disorders have the potential
to deteriorate the muscle condition additionally. Given these facts, idiopathic chronic
polyhydramnios is a rare opportunity to study the influence of reduced physical loading
on muscle development in the human fetus. Also, it could be a medical entity to examine
the influence of micro- and hypogravity conditions on the development of the fetal
muscular system during the last trimester of gestation.
PB  - Switzerland: Frontiers Media SA
T2  - Frontiers in Physiology
T1  - Chronic Polyhydramnios: A Medical Entity Which Could Be a Model of Muscle Development in Decreased Mechanical Loading Condition
VL  - 12
DO  - 10.3389/fphys.2021.810391
SP  - 810391
ER  - 

@article{
author = "Sekulić, Slobodan and Jakovljević, Branislava and Korovljev, Darinka and Simić, Svetlana and Čapo, Ivan and Podgorac, Jelena and Martać, Ljiljana and Kesić, Srđan and Rakić, Srđan and Petković, Branka",
year = "2022",
abstract = "Polyhydramnios is a condition related to an excessive accumulation of amniotic fluid
in the third trimester of pregnancy and it can be acute and chronic depending on
the duration. Published data suggest that during muscle development, in the stage
of late histochemical differentiation decreased mechanical loading cause decreased
expression of myosin heavy chain (MHC) type 1 leading to slow-to-fast transition. In the
case of chronic polyhydramnios, histochemical muscle differentiation could be affected
as a consequence of permanent decreased physical loading. Most affected would
be muscles which are the most active i.e., spine extensor muscles and muscles of
legs. Long-lasting decreased mechanical loading on muscle should cause decreased
expression of MHC type 1 leading to slow-to-fast transition, decreased number of
muscle fiber type I especially in extensor muscles of spine and legs. Additionally,
because MHC type 1 is present in all skeletal muscles it could lead to various degrees
of hypotrophy depending on constituting a percentage of MHC type 1 in affected
muscles. These changes in the case of preexisting muscle disorders have the potential
to deteriorate the muscle condition additionally. Given these facts, idiopathic chronic
polyhydramnios is a rare opportunity to study the influence of reduced physical loading
on muscle development in the human fetus. Also, it could be a medical entity to examine
the influence of micro- and hypogravity conditions on the development of the fetal
muscular system during the last trimester of gestation.",
publisher = "Switzerland: Frontiers Media SA",
journal = "Frontiers in Physiology",
title = "Chronic Polyhydramnios: A Medical Entity Which Could Be a Model of Muscle Development in Decreased Mechanical Loading Condition",
volume = "12",
doi = "10.3389/fphys.2021.810391",
pages = "810391"
}

Sekulić, S., Jakovljević, B., Korovljev, D., Simić, S., Čapo, I., Podgorac, J., Martać, L., Kesić, S., Rakić, S.,& Petković, B.. (2022). Chronic Polyhydramnios: A Medical Entity Which Could Be a Model of Muscle Development in Decreased Mechanical Loading Condition. in Frontiers in Physiology
Switzerland: Frontiers Media SA., 12, 810391.
https://doi.org/10.3389/fphys.2021.810391

Sekulić S, Jakovljević B, Korovljev D, Simić S, Čapo I, Podgorac J, Martać L, Kesić S, Rakić S, Petković B. Chronic Polyhydramnios: A Medical Entity Which Could Be a Model of Muscle Development in Decreased Mechanical Loading Condition. in Frontiers in Physiology. 2022;12:810391.
doi:10.3389/fphys.2021.810391 .

Sekulić, Slobodan, Jakovljević, Branislava, Korovljev, Darinka, Simić, Svetlana, Čapo, Ivan, Podgorac, Jelena, Martać, Ljiljana, Kesić, Srđan, Rakić, Srđan, Petković, Branka, "Chronic Polyhydramnios: A Medical Entity Which Could Be a Model of Muscle Development in Decreased Mechanical Loading Condition" in Frontiers in Physiology, 12 (2022):810391,
https://doi.org/10.3389/fphys.2021.810391 . .

TY  - CONF
AU  - Podgorac, Jelena
AU  - Petković, Branka
AU  - Martać, Ljiljana
AU  - Stojadinović, Gordana
AU  - Pešić, Vesna
AU  - Sekulić, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5745
AB  - Valproinska kiselina (VPK) je lek izbora u terapiji epilepsija kod trudnica, iako može uzrokovati različite poremećaje kod potomstva.1 Naša prethodna istraživanja su pokazala usporen somatski razvoj i odloženo sazrevanje motoričkog sistema u ranom postnatalnom periodu kod potomaka miševa koji su prenatalno kontinuirano izlagani VPK.2 Sa ciljem da proširimo saznanja vezana za model i procenimo dugotrajnost posledica VPK, u ovoj studiji ispitivali smo karakteristike psihomotornog ponašanja adolescentnih i odraslih mužjaka miševa u odgovoru na novu sredinu. Ženke su kontinuirano injecirane rastvorom VPK (50, 100 ili 400 mg/kg/dan; subkutano) tokom celog perioda gestacije. Potomstvo muškog pola starosti 6 nedelja (adolescenti) i 12 nedelja (odrasli) je ispitivano u testu otvorenog polja (open field test) u cilju procene lokomotorne, stereotipne i vertikalne aktivnosti. Rezultati ukazuju da tretman VPK: (1) nema uticaja na lokomotornu i vertikalnu aktivnost adolescentnih i odraslih jedinki, (2) smanjuje stereotipnu aktivnost adolescentnih jedinki u dozi od 100 i 400 mg/kg/dan. Dobijeni rezultati ukazuju da prenatalno kontinuirano izlaganje VPK ima dugotrajne posledice kod potomstva muškog pola, narušavajući očekivani odgovor adolescentnih jedinki na novu sredinu. Izostanak efekta kod odraslih jedinki se može objasniti spontanim dovođenjem sistema u ravnotežu usled maturacionih promena, što ipak ostaje da se dodatno proceni u drugim testovima zasnovanim na spontanom odgovoru na novu sredinu.
AB  - Валпроинска киселина (ВПК) је лек избора у терапији епилепсија код трудница,
иако може узроковати различите поремећаје код потомства.1 Наша претходна
истраживања су показала успорен соматски развој и одложено сазревање
моторичког система у раном постнаталном периоду код потомака мишева који су
пренатално континуирано излагани ВПК.2 Са циљем да проширимо сазнања везана
за модел и проценимо дуготрајност последица ВПК, у овој студији испитивали смо
карактеристике психомоторног понашања адолесцентних и одраслих мужјака
мишева у одговору на нову средину. Женке су континуирано ињециране раствором
ВПК (50, 100 или 400 мг/кг/дан; субкутано) током целог периода гестације.
Потомство мушког пола старости 6 недеља (адолесценти) и 12 недеља (одрасли) је
испитивано у тесту отвореног поља (open field test) у циљу процене локомоторне,
стереотипне и вертикалне активности. Резултати указују да третман ВПК: (1) нема
утицаја на локомоторну и вертикалну активност адолесцентних и одраслих
јединки, (2) смањује стереотипну активност адолесцентних јединки у дози од 100 и
400 мг/кг/дан. Добијени резултати указују да пренатално континуирано излагање
ВПК има дуготрајне последице код потомства мушког пола, нарушавајући
очекивани одговор адолесцентних јединки на нову средину. Изостанак ефекта код
одраслих јединки се може објаснити спонтаним довођењем система у равнотежу
услед матурационих промена, што ипак остаје да се додатно процени у другим
тестовима заснованим на спонтаном одговору на нову средину.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Uticaj kontinuiranog izlaganja valproinskoj kiselini tokom prenatalnog razvoja na psihomotorno ponašanje adolescentnih i odraslih mužjaka miševa
T1  - Утицај континуираног излагања валпроинској киселини током пренаталног развоја на психомоторно понашање адолесцентних и одраслих мужјака мишева
SP  - 369
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5745
ER  - 

@conference{
author = "Podgorac, Jelena and Petković, Branka and Martać, Ljiljana and Stojadinović, Gordana and Pešić, Vesna and Sekulić, Slobodan",
year = "2022",
abstract = "Valproinska kiselina (VPK) je lek izbora u terapiji epilepsija kod trudnica, iako može uzrokovati različite poremećaje kod potomstva.1 Naša prethodna istraživanja su pokazala usporen somatski razvoj i odloženo sazrevanje motoričkog sistema u ranom postnatalnom periodu kod potomaka miševa koji su prenatalno kontinuirano izlagani VPK.2 Sa ciljem da proširimo saznanja vezana za model i procenimo dugotrajnost posledica VPK, u ovoj studiji ispitivali smo karakteristike psihomotornog ponašanja adolescentnih i odraslih mužjaka miševa u odgovoru na novu sredinu. Ženke su kontinuirano injecirane rastvorom VPK (50, 100 ili 400 mg/kg/dan; subkutano) tokom celog perioda gestacije. Potomstvo muškog pola starosti 6 nedelja (adolescenti) i 12 nedelja (odrasli) je ispitivano u testu otvorenog polja (open field test) u cilju procene lokomotorne, stereotipne i vertikalne aktivnosti. Rezultati ukazuju da tretman VPK: (1) nema uticaja na lokomotornu i vertikalnu aktivnost adolescentnih i odraslih jedinki, (2) smanjuje stereotipnu aktivnost adolescentnih jedinki u dozi od 100 i 400 mg/kg/dan. Dobijeni rezultati ukazuju da prenatalno kontinuirano izlaganje VPK ima dugotrajne posledice kod potomstva muškog pola, narušavajući očekivani odgovor adolescentnih jedinki na novu sredinu. Izostanak efekta kod odraslih jedinki se može objasniti spontanim dovođenjem sistema u ravnotežu usled maturacionih promena, što ipak ostaje da se dodatno proceni u drugim testovima zasnovanim na spontanom odgovoru na novu sredinu., Валпроинска киселина (ВПК) је лек избора у терапији епилепсија код трудница,
иако може узроковати различите поремећаје код потомства.1 Наша претходна
истраживања су показала успорен соматски развој и одложено сазревање
моторичког система у раном постнаталном периоду код потомака мишева који су
пренатално континуирано излагани ВПК.2 Са циљем да проширимо сазнања везана
за модел и проценимо дуготрајност последица ВПК, у овој студији испитивали смо
карактеристике психомоторног понашања адолесцентних и одраслих мужјака
мишева у одговору на нову средину. Женке су континуирано ињециране раствором
ВПК (50, 100 или 400 мг/кг/дан; субкутано) током целог периода гестације.
Потомство мушког пола старости 6 недеља (адолесценти) и 12 недеља (одрасли) је
испитивано у тесту отвореног поља (open field test) у циљу процене локомоторне,
стереотипне и вертикалне активности. Резултати указују да третман ВПК: (1) нема
утицаја на локомоторну и вертикалну активност адолесцентних и одраслих
јединки, (2) смањује стереотипну активност адолесцентних јединки у дози од 100 и
400 мг/кг/дан. Добијени резултати указују да пренатално континуирано излагање
ВПК има дуготрајне последице код потомства мушког пола, нарушавајући
очекивани одговор адолесцентних јединки на нову средину. Изостанак ефекта код
одраслих јединки се може објаснити спонтаним довођењем система у равнотежу
услед матурационих промена, што ипак остаје да се додатно процени у другим
тестовима заснованим на спонтаном одговору на нову средину.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Uticaj kontinuiranog izlaganja valproinskoj kiselini tokom prenatalnog razvoja na psihomotorno ponašanje adolescentnih i odraslih mužjaka miševa, Утицај континуираног излагања валпроинској киселини током пренаталног развоја на психомоторно понашање адолесцентних и одраслих мужјака мишева",
pages = "369",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5745"
}

Podgorac, J., Petković, B., Martać, L., Stojadinović, G., Pešić, V.,& Sekulić, S.. (2022). Uticaj kontinuiranog izlaganja valproinskoj kiselini tokom prenatalnog razvoja na psihomotorno ponašanje adolescentnih i odraslih mužjaka miševa. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 369.
https://hdl.handle.net/21.15107/rcub_ibiss_5745

Podgorac J, Petković B, Martać L, Stojadinović G, Pešić V, Sekulić S. Uticaj kontinuiranog izlaganja valproinskoj kiselini tokom prenatalnog razvoja na psihomotorno ponašanje adolescentnih i odraslih mužjaka miševa. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:369.
https://hdl.handle.net/21.15107/rcub_ibiss_5745 .

Podgorac, Jelena, Petković, Branka, Martać, Ljiljana, Stojadinović, Gordana, Pešić, Vesna, Sekulić, Slobodan, "Uticaj kontinuiranog izlaganja valproinskoj kiselini tokom prenatalnog razvoja na psihomotorno ponašanje adolescentnih i odraslih mužjaka miševa" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):369,
https://hdl.handle.net/21.15107/rcub_ibiss_5745 .

TY  - CONF
AU  - Podgorac, Jelena
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Sekulić, Slobodan
AU  - Pešić, Vesna
PY  - 2022
UR  - https://kenesvm.azureedge.net/public/general/FENS2022.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5734
AB  - Aims: Levetiracetam (LEV), which belongs to a new generation of antiepileptic drugs, has been recognized as a relatively safe antiepileptic therapy, according to studies on neurodevelopmental outcomes in children exposed to LEV in utero, although some animal studies reported skeletal abnormalities. This study deals with the influence of prenatal exposure to LEV in the doses that cover therapeutic range in humans on gross skeletal structure and anxiety-like behavior in adolescent mice. Methods: Adult 8-weeks old female NMRI mice were randomly divided into four groups and treated subcutaneously during breeding and gestation with saline (control) or LEV in the doses of 158 mg/kg/day (LEV-158), 211 mg/kg/day (LEV-211), or 316 mg/kg/day (LEV-316). After delivery, each female with the progeny was housed separately. Weaning and separation by sex were done on the 20th postnatal day (P20). The elevated plus-maze test was used to assess the anxiety-like behavior in both male and female offspring on P37. Results: Females and males in the LEV-158 and LEV-316 group displayed less anxiety-like behavior compared to the control, while in the LEV-211 group such behavior was not observed. Sex-related differences in anxiety-like behavior were not detected within any LEV group, as well as visible skeletal malformations. Conclusion: The findings in the mouse model suggest that prenatal exposure to LEV could be associated with less anxiety-like behavior in adolescence, paying attention to the U-shaped dose-response and highlighting the behavioral outcomes of small LEV doses.
PB  - Federation of European Neuroscience Societies
C3  - E-Book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
T1  - Anxiety-like behavior in adolescent mice prenatally exposed to different doses of levetiracetam
SP  - S02-173
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5734
ER  - 

@conference{
author = "Podgorac, Jelena and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Sekulić, Slobodan and Pešić, Vesna",
year = "2022",
abstract = "Aims: Levetiracetam (LEV), which belongs to a new generation of antiepileptic drugs, has been recognized as a relatively safe antiepileptic therapy, according to studies on neurodevelopmental outcomes in children exposed to LEV in utero, although some animal studies reported skeletal abnormalities. This study deals with the influence of prenatal exposure to LEV in the doses that cover therapeutic range in humans on gross skeletal structure and anxiety-like behavior in adolescent mice. Methods: Adult 8-weeks old female NMRI mice were randomly divided into four groups and treated subcutaneously during breeding and gestation with saline (control) or LEV in the doses of 158 mg/kg/day (LEV-158), 211 mg/kg/day (LEV-211), or 316 mg/kg/day (LEV-316). After delivery, each female with the progeny was housed separately. Weaning and separation by sex were done on the 20th postnatal day (P20). The elevated plus-maze test was used to assess the anxiety-like behavior in both male and female offspring on P37. Results: Females and males in the LEV-158 and LEV-316 group displayed less anxiety-like behavior compared to the control, while in the LEV-211 group such behavior was not observed. Sex-related differences in anxiety-like behavior were not detected within any LEV group, as well as visible skeletal malformations. Conclusion: The findings in the mouse model suggest that prenatal exposure to LEV could be associated with less anxiety-like behavior in adolescence, paying attention to the U-shaped dose-response and highlighting the behavioral outcomes of small LEV doses.",
publisher = "Federation of European Neuroscience Societies",
journal = "E-Book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France",
title = "Anxiety-like behavior in adolescent mice prenatally exposed to different doses of levetiracetam",
pages = "S02-173",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5734"
}

Podgorac, J., Petković, B., Stojadinović, G., Martać, L., Sekulić, S.,& Pešić, V.. (2022). Anxiety-like behavior in adolescent mice prenatally exposed to different doses of levetiracetam. in E-Book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
Federation of European Neuroscience Societies., S02-173.
https://hdl.handle.net/21.15107/rcub_ibiss_5734

Podgorac J, Petković B, Stojadinović G, Martać L, Sekulić S, Pešić V. Anxiety-like behavior in adolescent mice prenatally exposed to different doses of levetiracetam. in E-Book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. 2022;:S02-173.
https://hdl.handle.net/21.15107/rcub_ibiss_5734 .

Podgorac, Jelena, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Sekulić, Slobodan, Pešić, Vesna, "Anxiety-like behavior in adolescent mice prenatally exposed to different doses of levetiracetam" in E-Book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France (2022):S02-173,
https://hdl.handle.net/21.15107/rcub_ibiss_5734 .

TY  - JOUR
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Petković, Branka
AU  - Stojadinović, Gordana
PY  - 2022
UR  - https://www.primescholars.com/articles/aluminium-neurotoxicity-and-neuroprotection.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5736
AB  - Aluminium is considered to be the most widely distributed metal in nature and industry and is extensively used in products and processes associated with human activity. Contamination may occur by air, water, food, additives, medicaments, vaccines, cosmetics, agrochemicals, etc. Aluminium is recognized as a highly neurotoxic element in animals and humans connected with several diseases such as Alzheimer’s and Parkinson’s disease, neurodegenerative motor disorders, encephalopathy, dementia, amyotrophic lateral sclerosis, multiple sclerosis, and autism. There are many animal models in rats developed to investigate aluminium neurotoxicity. Nevertheless, molecular mechanisms of its action are not yet resolved, and mechanisms of damage and safety concentrations are still much discussed. The brain is the most susceptible system to damages provoked by aluminium exposure, such as oxidative stress, iron dyshomeostasis, changes in neurotransmission, immunologic alteration and pro-inflammation, genotoxicity, transformation and peptide denaturation, changes in enzyme activity, membrane perturbation, apoptosis, necrosis, and dysplasia. A novel investigation of aluminium neurotoxicity includes the assessment of neuroprotection and the identification of new substances as potential drugs.
PB  - London: Prime Scholars
T2  - Journal of Heavy Metal Toxicity and Diseases
T1  - Aluminium neurotoxicity and neuroprotection
IS  - 4
VL  - 7
DO  - 10.21767/2473-6457.22.7.4.11
SP  - 11
ER  - 

@article{
author = "Martać, Ljiljana and Podgorac, Jelena and Petković, Branka and Stojadinović, Gordana",
year = "2022",
abstract = "Aluminium is considered to be the most widely distributed metal in nature and industry and is extensively used in products and processes associated with human activity. Contamination may occur by air, water, food, additives, medicaments, vaccines, cosmetics, agrochemicals, etc. Aluminium is recognized as a highly neurotoxic element in animals and humans connected with several diseases such as Alzheimer’s and Parkinson’s disease, neurodegenerative motor disorders, encephalopathy, dementia, amyotrophic lateral sclerosis, multiple sclerosis, and autism. There are many animal models in rats developed to investigate aluminium neurotoxicity. Nevertheless, molecular mechanisms of its action are not yet resolved, and mechanisms of damage and safety concentrations are still much discussed. The brain is the most susceptible system to damages provoked by aluminium exposure, such as oxidative stress, iron dyshomeostasis, changes in neurotransmission, immunologic alteration and pro-inflammation, genotoxicity, transformation and peptide denaturation, changes in enzyme activity, membrane perturbation, apoptosis, necrosis, and dysplasia. A novel investigation of aluminium neurotoxicity includes the assessment of neuroprotection and the identification of new substances as potential drugs.",
publisher = "London: Prime Scholars",
journal = "Journal of Heavy Metal Toxicity and Diseases",
title = "Aluminium neurotoxicity and neuroprotection",
number = "4",
volume = "7",
doi = "10.21767/2473-6457.22.7.4.11",
pages = "11"
}

Martać, L., Podgorac, J., Petković, B.,& Stojadinović, G.. (2022). Aluminium neurotoxicity and neuroprotection. in Journal of Heavy Metal Toxicity and Diseases
London: Prime Scholars., 7(4), 11.
https://doi.org/10.21767/2473-6457.22.7.4.11

Martać L, Podgorac J, Petković B, Stojadinović G. Aluminium neurotoxicity and neuroprotection. in Journal of Heavy Metal Toxicity and Diseases. 2022;7(4):11.
doi:10.21767/2473-6457.22.7.4.11 .

Martać, Ljiljana, Podgorac, Jelena, Petković, Branka, Stojadinović, Gordana, "Aluminium neurotoxicity and neuroprotection" in Journal of Heavy Metal Toxicity and Diseases, 7, no. 4 (2022):11,
https://doi.org/10.21767/2473-6457.22.7.4.11 . .

TY  - JOUR
AU  - Petković, Branka
AU  - Kesić, Srđan
AU  - Ristić, Slavica
AU  - Pavković, Željko
AU  - Podgorac, Jelena
AU  - Stojadinović, Gordana
AU  - Pešić, Vesna
PY  - 2020
UR  - https://www.eurekaselect.com/180519/article
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32213154
UR  - https://radar.ibiss.bg.ac.rs/123456789/3879
AB  - BACKGROUND Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy. OBJECTIVE We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal. METHOD The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals' motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too. RESULTS The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested. CONCLUSION Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.
PB  - Bentham Science Publishers Ltd.
T2  - Current Pharmaceutical Design
T1  - A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats
IS  - 31
VL  - 26
DO  - 10.2174/1381612826666200326125821
SP  - 3884
EP  - 3894
ER  - 

@article{
author = "Petković, Branka and Kesić, Srđan and Ristić, Slavica and Pavković, Željko and Podgorac, Jelena and Stojadinović, Gordana and Pešić, Vesna",
year = "2020",
abstract = "BACKGROUND Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy. OBJECTIVE We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal. METHOD The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals' motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too. RESULTS The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested. CONCLUSION Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Pharmaceutical Design",
title = "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats",
number = "31",
volume = "26",
doi = "10.2174/1381612826666200326125821",
pages = "3884-3894"
}

Petković, B., Kesić, S., Ristić, S., Pavković, Ž., Podgorac, J., Stojadinović, G.,& Pešić, V.. (2020). A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design
Bentham Science Publishers Ltd.., 26(31), 3884-3894.
https://doi.org/10.2174/1381612826666200326125821

Petković B, Kesić S, Ristić S, Pavković Ž, Podgorac J, Stojadinović G, Pešić V. A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design. 2020;26(31):3884-3894.
doi:10.2174/1381612826666200326125821 .

Petković, Branka, Kesić, Srđan, Ristić, Slavica, Pavković, Željko, Podgorac, Jelena, Stojadinović, Gordana, Pešić, Vesna, "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats" in Current Pharmaceutical Design, 26, no. 31 (2020):3884-3894,
https://doi.org/10.2174/1381612826666200326125821 . .

TY  - JOUR
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Spasić, Slađana Z.
AU  - Petković, Branka
AU  - Sekulić, Slobodan
AU  - Kesić, Srđan
PY  - 2020
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5405
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4005
AB  - A detailed analysis of the anesthetic-induced modulation of intracerebellar electrical activity is an important step to understand the functional brain responses to anesthesia. We examined the electrical activity recorded from different cortical layers: molecular layer (ML), Purkinje cell layer (PCL), granular layer (GL) and the white matter (WM) in the vermian part of rat cerebellar lobule V during Nembutal anesthesia using spectral and fractal analysis. Spectral analysis revealed a difference in the mean relative power of delta (0.1-4.0 Hz) and theta (4.1-8.0 Hz) frequencies through the cerebellar layers. Compared to the ML, delta activity increased significantly in the GL, while theta activity decreased in the GL and the WM. Fractal analysis revealed that the mean value of Higuchi’s fractal dimension (HFD) increased, starting from the ML to the WM. Theta activity exhibited a negative correlation with the HFD value in the ML. In contrast, the gamma activity showed a positive correlation with the HFD value in the ML and the GL. The combined use of spectral and fractal analyses revealed that Nembutal displays different effects on rat cerebellar electrical activity, which largely depends on the neurochemical and electrophysiological organization of the cerebellar layers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis
IS  - 3
VL  - 72
DO  - 10.2298/ABS200524036S
SP  - 425
EP  - 432
ER  - 

@article{
author = "Stojadinović, Gordana and Martać, Ljiljana and Podgorac, Jelena and Spasić, Slađana Z. and Petković, Branka and Sekulić, Slobodan and Kesić, Srđan",
year = "2020",
abstract = "A detailed analysis of the anesthetic-induced modulation of intracerebellar electrical activity is an important step to understand the functional brain responses to anesthesia. We examined the electrical activity recorded from different cortical layers: molecular layer (ML), Purkinje cell layer (PCL), granular layer (GL) and the white matter (WM) in the vermian part of rat cerebellar lobule V during Nembutal anesthesia using spectral and fractal analysis. Spectral analysis revealed a difference in the mean relative power of delta (0.1-4.0 Hz) and theta (4.1-8.0 Hz) frequencies through the cerebellar layers. Compared to the ML, delta activity increased significantly in the GL, while theta activity decreased in the GL and the WM. Fractal analysis revealed that the mean value of Higuchi’s fractal dimension (HFD) increased, starting from the ML to the WM. Theta activity exhibited a negative correlation with the HFD value in the ML. In contrast, the gamma activity showed a positive correlation with the HFD value in the ML and the GL. The combined use of spectral and fractal analyses revealed that Nembutal displays different effects on rat cerebellar electrical activity, which largely depends on the neurochemical and electrophysiological organization of the cerebellar layers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis",
number = "3",
volume = "72",
doi = "10.2298/ABS200524036S",
pages = "425-432"
}

Stojadinović, G., Martać, L., Podgorac, J., Spasić, S. Z., Petković, B., Sekulić, S.,& Kesić, S.. (2020). The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis. in Archives of Biological Sciences
Serbian Biological Society., 72(3), 425-432.
https://doi.org/10.2298/ABS200524036S

Stojadinović G, Martać L, Podgorac J, Spasić SZ, Petković B, Sekulić S, Kesić S. The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis. in Archives of Biological Sciences. 2020;72(3):425-432.
doi:10.2298/ABS200524036S .

Stojadinović, Gordana, Martać, Ljiljana, Podgorac, Jelena, Spasić, Slađana Z., Petković, Branka, Sekulić, Slobodan, Kesić, Srđan, "The effects of Nembutal on the intracerebellar EEG activity revealed by spectral and fractal analysis" in Archives of Biological Sciences, 72, no. 3 (2020):425-432,
https://doi.org/10.2298/ABS200524036S . .

TY  - JOUR
AU  - Petković, Branka
AU  - Kesić, Srđan
AU  - Ristić, Slavica
AU  - Pavković, Željko
AU  - Podgorac, Jelena
AU  - Stojadinović, Gordana
AU  - Pešić, Vesna
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3765
AB  - Background: Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy.
Objective: We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal.
Method: The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals’ motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too.
Results: The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested.
Conclusion: Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.
PB  - Bentham Science Publishers Ltd.
T2  - Current Pharmaceutical Design
T1  - A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats
DO  - 10.2174/1381612826666200326125821
ER  - 

@article{
author = "Petković, Branka and Kesić, Srđan and Ristić, Slavica and Pavković, Željko and Podgorac, Jelena and Stojadinović, Gordana and Pešić, Vesna",
year = "2020",
abstract = "Background: Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy.
Objective: We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal.
Method: The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals’ motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too.
Results: The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested.
Conclusion: Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Pharmaceutical Design",
title = "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats",
doi = "10.2174/1381612826666200326125821"
}

Petković, B., Kesić, S., Ristić, S., Pavković, Ž., Podgorac, J., Stojadinović, G.,& Pešić, V.. (2020). A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design
Bentham Science Publishers Ltd...
https://doi.org/10.2174/1381612826666200326125821

Petković B, Kesić S, Ristić S, Pavković Ž, Podgorac J, Stojadinović G, Pešić V. A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design. 2020;.
doi:10.2174/1381612826666200326125821 .

Petković, Branka, Kesić, Srđan, Ristić, Slavica, Pavković, Željko, Podgorac, Jelena, Stojadinović, Gordana, Pešić, Vesna, "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats" in Current Pharmaceutical Design (2020),
https://doi.org/10.2174/1381612826666200326125821 . .

TY  - JOUR
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Kesić, Srđan
AU  - Ristić, Slavica
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900018P
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3400
AB  - Clinically-related basic studies on the behavioral effects of ribavirin treatment are still lacking despite its wide use as an antiviral medication. This paper considers the effects of low ribavirin doses (10, 20 and 30 mg/kg/day) on psychomotor activity (novelty-induced exploratory behavior, d-amphetamine (AMPH, 1.5 mg/kg, intraperitoneal)-induced motor activity), and body weight gain in socially undisturbed adult male Wistar rats 24 h after the first, seventh and fourteenth once-a-day injection. Low doses of ribavirin were tested in an attempt to avoid the recognized systemic side effects related to high-dose usage. None of the singly applied ribavirin doses affected exploratory/spontaneous and AMPH-induced motor behavior (locomotion, stereotypy-like and vertical activity), however, body weight gain was significantly lower after treatment with 30 mg/kg of ribavirin. The 7- and 14-day treatments with 10 and 30 mg/kg/day of ribavirin significantly suppressed novelty-induced locomotion and body weight gain; the 14-day treatment with ribavirin at a dose of 30 mg/kg/ day decreased AMPH-induced stereotypy. These findings indicate that repeated application (up to 14 days) of low ribavirin doses results in low novelty-induced locomotion along with reduced weight gain, accentuating the existence of a U-shaped dose-response relationship with a prolonged duration of ribavirin treatment.
T2  - Archives of Biological Sciences
T1  - Psychomotor activity and body weight gain after exposure to low ribavirin doses in rats: role of treatment duration
IS  - 2
VL  - 71
DO  - 10.2298/ABS190205018P
SP  - 357
EP  - 368
ER  - 

@article{
author = "Petković, Branka and Stojadinović, Gordana and Kesić, Srđan and Ristić, Slavica and Martać, Ljiljana and Podgorac, Jelena and Pešić, Vesna",
year = "2019",
abstract = "Clinically-related basic studies on the behavioral effects of ribavirin treatment are still lacking despite its wide use as an antiviral medication. This paper considers the effects of low ribavirin doses (10, 20 and 30 mg/kg/day) on psychomotor activity (novelty-induced exploratory behavior, d-amphetamine (AMPH, 1.5 mg/kg, intraperitoneal)-induced motor activity), and body weight gain in socially undisturbed adult male Wistar rats 24 h after the first, seventh and fourteenth once-a-day injection. Low doses of ribavirin were tested in an attempt to avoid the recognized systemic side effects related to high-dose usage. None of the singly applied ribavirin doses affected exploratory/spontaneous and AMPH-induced motor behavior (locomotion, stereotypy-like and vertical activity), however, body weight gain was significantly lower after treatment with 30 mg/kg of ribavirin. The 7- and 14-day treatments with 10 and 30 mg/kg/day of ribavirin significantly suppressed novelty-induced locomotion and body weight gain; the 14-day treatment with ribavirin at a dose of 30 mg/kg/ day decreased AMPH-induced stereotypy. These findings indicate that repeated application (up to 14 days) of low ribavirin doses results in low novelty-induced locomotion along with reduced weight gain, accentuating the existence of a U-shaped dose-response relationship with a prolonged duration of ribavirin treatment.",
journal = "Archives of Biological Sciences",
title = "Psychomotor activity and body weight gain after exposure to low ribavirin doses in rats: role of treatment duration",
number = "2",
volume = "71",
doi = "10.2298/ABS190205018P",
pages = "357-368"
}

Petković, B., Stojadinović, G., Kesić, S., Ristić, S., Martać, L., Podgorac, J.,& Pešić, V.. (2019). Psychomotor activity and body weight gain after exposure to low ribavirin doses in rats: role of treatment duration. in Archives of Biological Sciences, 71(2), 357-368.
https://doi.org/10.2298/ABS190205018P

Petković B, Stojadinović G, Kesić S, Ristić S, Martać L, Podgorac J, Pešić V. Psychomotor activity and body weight gain after exposure to low ribavirin doses in rats: role of treatment duration. in Archives of Biological Sciences. 2019;71(2):357-368.
doi:10.2298/ABS190205018P .

Petković, Branka, Stojadinović, Gordana, Kesić, Srđan, Ristić, Slavica, Martać, Ljiljana, Podgorac, Jelena, Pešić, Vesna, "Psychomotor activity and body weight gain after exposure to low ribavirin doses in rats: role of treatment duration" in Archives of Biological Sciences, 71, no. 2 (2019):357-368,
https://doi.org/10.2298/ABS190205018P . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5456
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5455
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Petković, Branka
AU  - Sekulić, Slobodan
AU  - Čapo, Ivan
PY  - 2015
UR  - http://arpgweb.com/?ic=journal&journal=16&info=aims
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3777
AB  - This paper presents an overview of rat animal model of Alzheimer’s disease. Model is based on the
toxicity, oxidative stress and neurodegeneration. The model is obtained by treatment of rats with intraperitoneal aluminum. Electrocortical group neuron activity describes changes in neurotransmission caused by different factors. Such changes could be qualitatively described by spectral and fractal analysis of electrocortical activity as a variation of the relative spectral power. Both animals and patients with Alzheimers demention have increased relative spectral power in delta range. By fractal analysis we described changes in electrocortical activity of aluminum intoxication animals compare to physiological control. We used change in delta range to calculate fractal dimension. Also we used fractal dimension to compare treated animals with control ones to quantitatively describe the degree of pathophysiological state. All changes are correlated with an increase in the relative spectral power in the delta range and can be quantitatively described by fractal dimension. Results are presented as the effect and can also be applied to the human model of neurotoxicity and neurodegeneration like Alzheimer’s dementia. The model itself may be used for diagnostic and prognostic purposes since it describes the parameters underlying Alzheimer’s disease. However in definition of disease should be consider a state of dementia and menthal activity.
PB  - Academic Research Publishing Group
T2  - Journal of Biotechnology Research
T1  - Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication
IS  - 5
VL  - 1
SP  - 21
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3777
ER  - 

@article{
author = "Martać, Ljiljana and Podgorac, Jelena and Petković, Branka and Sekulić, Slobodan and Čapo, Ivan",
year = "2015",
abstract = "This paper presents an overview of rat animal model of Alzheimer’s disease. Model is based on the
toxicity, oxidative stress and neurodegeneration. The model is obtained by treatment of rats with intraperitoneal aluminum. Electrocortical group neuron activity describes changes in neurotransmission caused by different factors. Such changes could be qualitatively described by spectral and fractal analysis of electrocortical activity as a variation of the relative spectral power. Both animals and patients with Alzheimers demention have increased relative spectral power in delta range. By fractal analysis we described changes in electrocortical activity of aluminum intoxication animals compare to physiological control. We used change in delta range to calculate fractal dimension. Also we used fractal dimension to compare treated animals with control ones to quantitatively describe the degree of pathophysiological state. All changes are correlated with an increase in the relative spectral power in the delta range and can be quantitatively described by fractal dimension. Results are presented as the effect and can also be applied to the human model of neurotoxicity and neurodegeneration like Alzheimer’s dementia. The model itself may be used for diagnostic and prognostic purposes since it describes the parameters underlying Alzheimer’s disease. However in definition of disease should be consider a state of dementia and menthal activity.",
publisher = "Academic Research Publishing Group",
journal = "Journal of Biotechnology Research",
title = "Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication",
number = "5",
volume = "1",
pages = "21-25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3777"
}

Martać, L., Podgorac, J., Petković, B., Sekulić, S.,& Čapo, I.. (2015). Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication. in Journal of Biotechnology Research
Academic Research Publishing Group., 1(5), 21-25.
https://hdl.handle.net/21.15107/rcub_ibiss_3777

Martać L, Podgorac J, Petković B, Sekulić S, Čapo I. Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication. in Journal of Biotechnology Research. 2015;1(5):21-25.
https://hdl.handle.net/21.15107/rcub_ibiss_3777 .

Martać, Ljiljana, Podgorac, Jelena, Petković, Branka, Sekulić, Slobodan, Čapo, Ivan, "Spectral and Fractal Analysis of ECoG in Animal Model of Aluminium Intoxication" in Journal of Biotechnology Research, 1, no. 5 (2015):21-25,
https://hdl.handle.net/21.15107/rcub_ibiss_3777 .

TY  - THES
AU  - Podgorac, Jelena
PY  - 2014
UR  - http://eteze.kg.ac.rs/application/showtheses?thesesId=1324
UR  - https://fedorakg.kg.ac.rs/fedora/get/o:409/bdef:Content/download
UR  - http://nardus.mpn.gov.rs/123456789/3741
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2441
AB  - Osnovni cilj ove doktorske disertacije je bio da se ispitaju posledice intrauterinog izlaganja različitim dozama valproata na tok fizičkog i psihomotornog razvoja jedinki miša. Ispitivani su efekti valproata na CNS-u u subterapijskim dozama i dozno- zavisni efekat. Testovi primenjeni na potomstvu su u literaturi okarakterisani kao odgovarajući za procenu stanja motornog i senzornog sistema u određenoj fazi postnatalnog razvoja. Određeni testovi, pored opšte motorne sposobnosti, omogućavali su i procenu ponašanja tipa anksioznosti / depresije, hiper- i hipoaktivnosti jedinki.
Metode. U eksperimentima su korišćene ženke i mužjaci zdravih laboratorijskih miševa soja NMRI, odabrane metodom slučajnog izbora iz okota na Vojnomedicinskoj akademiji u Beogradu. Eksperiment je počinjao sparivanjem dve ženke i jednog mužjaka u jednom kavezu. Shodno tretmanu, oformljeno je 5 eksperimentalnih grupa i to jedna kontrolna (n=9 ženki), koja je bila tretirana fiziološkim rastvorom (Natrii Chloridi Infundibule 0,9%, HemofarmhospitalLogica), i 4 grupe koje su tretirane valproatom (Valproic acid sodium salt – 2 propylpentanoic sodium, ≥98, P4543 Sigma Aldrich) u dozi od 50 mg/kg, 100 mg/kg, 200 mg/kg ili 400 mg/kg (n=10-11 ženki po grupi). Natrijumova so valproinske kiseline rastvarana je u fiziološkom rastvoru. Injeciranje je obavljano svakodnevno, od momenta sparivanja, tokom čitavog perioda priploda i gestacije. Nakon rađanja potomstva tretman je prestajao i ženke su sa svojim potomstvom odvajane u zasebne kaveze. Pristup hrani i vodi je bio neograničen.
Procenjivan je odgovor potomstva u sledećim testovima: (1) test refleksa uspravljanja , (2)test kačenja o rep, (3) test izlaganja vrućoj ploči, (4) test uzdignutog krstastog lavirinta, (5) test otvorenog polja
Rezultati. Kontinuirana primena različitih doza valproata (50, 100, 200 i 400 mg/kg) na ženkama miša NMRI soja u periodu priploda ima negativan dozno-zavisni uticaj na fertilitet, a kontinuirana primena u periodu priploda i gestacije na smrtnost ženki (akcenat na visokim dozama). Tretman nema značajnog uticaja na telesnu težinu ženki.
Intrauterino izlaganje valproatu ima negativan uticaj na telesnu težinu jedinki tokom postnatalnog razvoja, uključujući period adolescencije. Tretman je povezan i sa kašnjenjem u otvaranju očiju jedinki u periodu do 15. dana postanatalnog razvoja.
Intrauterino izlaganje različitim dozama valproata značajno utiče na ponašanje jedinki u testu uspravljanja. Shodno starosti jedinki efekat tretmana se ogleda kako u kvalitetu (tipu) odgovora i u vremenu koje je potrebno jedinki da se uspravi.
Tretman valproatom tokom prenatalnog perioda utiče na ishod testa kačenja o rep kada se jedinke testiraju u periodu od 5. do 10. postnatalnog dana (sa akcentom na doze 200 mg/kg i 400 mg/kg). Kod jedinki starih 15 postnatalnih dana posledice tretmana nisu uočene.
Intrauterino izlaganje valproatu značajno utiče na ishod u testu izlaganja vrućoj ploči jedinki starih 25 i 32 postnatalna dana. Efekat se ogleda u povećanju broja jedinki koje neadekvatno odgovaraju na test, kao i u produženoj latenci odgovora na stimulus kod jedinki koje adekvatno odgovore na test.
Izlaganje majki tokom trudnoće valproatu značajno utiče na ponašanje adolescentnog potomstva (PND35) u testu uzdignutog krstastog lavirinta, a posledice su dozno- i polno-specifične. U testu otvorenog polja (PND40), izlaganje majki tokom trudnoće valproatu značajno utiče na ponašanje adolescentnog potomstva oba pola. Shodno primenjenoj dozi, efekat se ogleda u finom narušavanju analiziranih parametara (lokomotorna, stereotipna ili vertikalna aktivnost) u određenom periodu boravka u otvorenom polju.
Zaključak. Primenjeni eksperimentalni model kontirnuirane aplikacije valproata tokom priploda i gestacije na jedinkama miša je u korelaciji sa njegovom terapijskom primenom u tretmanu epilepsija tokom trudnoće u humanoj populaciji. Rezultati dobijeni u našim istraživanjima su jasno ukazali da posledice intrauterinog izlaganja valproatu zavise kako od primenjene doze tako i od pola potomstva. Od posebnog značaja su pokazatelji da subterapijske doze valproata primenjene tokom gestacije značajno utiču na psihomotorni razvoj jedinki. U metodološkom smislu naši eksperimenti su pokazali da za pravilno sagledavanje posledica intrauterinog izlaganja valproatu mora da postoji adekvatan opseg postnatalnih starosti jedinki. Shodno činjenici da je metabolizam valproata kod jedinki miševa ubrzan u odnosu na metabolizam ovog leka u humanoj populaciji, kritički pristup u aproksimaciji podataka dobijenih iz animalnog modela miša na ljudsku populaciju je neophodan.
Cilj ove doktorske disertacije je bio da se ispitaju posledice intrauterinog izlaganja različitim dozama valproata (sa akcentom na subterapijske doze) na psihomotorni razvoj jedinki miša, koje su testirane u različitim fazama postnatalnog razvoja. Baterija testova za ispitivanje ponašanja koja je upotrebljena tokom ove studije uključivala je sledeće testove: test refleksa uspravljanja i test kačenja o rep – tokom prve dve postnatalne nedelje, test izlaganja vrućoj ploči – u periadolescentnoj fazi, test uzdignutog krstastog lavirinta i test otvorenog polja – tokom adolescencije.
Podaci dobijeni u testu refleksa uspravljanja i testu kačenja jedinke o rep su jasno ukazali na kašnjenje u razvoju senzornog i motornog sistema jedinki koje su tokom gestacije bile izložene različitim dozama valproata, koje je uočljivo kroz kvalitativno i kvantitativno drugačiji odgovor jedinki na date testove u periodu ranog postnatalnog razvoja. Velike fizičke anomalije nisu detektovane, ali je jasno pokazan negativan uticaj intrauterinog izlaganja valproatu na telesnu težinu i otvaranje očiju. Podaci dobijeni u testu vruće ploče su dodatno ukazali na kašnjenje u sazrevanju senzornog sistema značajnog za percepciju bolnog stimulusa, sa posebnim akcentom na uticaj malih
5
doza valproata. Testovi primenjeni na jedinkama u periodu adolescencije su ukazali da intrauterino izlaganje valproatu izaziva polno- i dozno-specifične promene u ponašanju, u smislu anksiozno/depresivnih stanja kod jedinki muškog pola usled primene velikih doza valproata i hiperaktivnih stanja kod jedinki ženskog pola usled primene malih doza valproata.
Rezultati dobijeni u ovom istraživanju pružaju mogućnost sagledavanja posledica primene valproata tokom trudnoće na funkcionisanje centralnog nervnog sistema potomstva, sa aspekta ponašanja. U skladu sa literaturom, naši eksperimenti su ukazali da intrauterino izlaganje valproatu izaziva kompleksne promene u ponašanju potomstva, usled morfo-funkcionalnih poremećaja u određenim moždanim regionima. Ipak, ostaje da se precizno utvrde neuralni korelati bihejvioralnih efekata opisanih u našoj studiji koja je za model imala kontinuiranu primenu valproata tokom čitave gestacije, što je eksperimentalno za sada nedovoljno ispitano.
AB  - The aim of this study was to examine the effects of intrauterine exposure to different doses of valproate on the course of physical and psychomotor development of mouse. The effects of valproate in the CNS was evaluated at subtherapeutic doses and dose-dependent effect. 
The tests applied to the offspring in the literature are labeled as suitable for the assessment of motor and sensory system at a certain stage of postnatal development. Certain tests, in addition to general motor skills, allowed the assessment of behavioral state as anxiety / depression, hyper-and hypo-activity of offspring. 
The study was done on adult female NMRI mice. Four experimental and one control group was formed. Females were treated with subcutaneous injection of 50, 100, 200 and 400 mg/kg VPA (Valproic acid sodium salt – 2 propylpentanoic sodium, ≥98, P4543 Sigma Aldrich) or with saline (Natrii Chloridi Infundibule 0,9%, HemofarmhospitalLogica) (control group). All treatments were performed during breeding and whole gestation period. Mice were given ad libitum access to food and water. The offspring was examined in tests: 
(1) Righting test, (2) Tail suspension test, (3) Hot plate test, (4) Elevated plus maze test and (5) Open field test 
Results: Different doses of VPA (50, 100, 200 and 400 mg/kg) continuously applied on female NMRI mice during breeding and whole gestation caused female mortality and lower fertility dose-dependent effect. Treatment had no influence on female body weight. 
In utero exposure to valproate has a negative impact on body weight of individuals during postnatal development, including the adolescence. Treatment is associated with a delay in eye-opening at the 15th postnatal day (PND). 
Prenatal exposition to different doses of VPA significantly influence on offspring behaviour in righting reflex. Regarding the critical developmental postnatal points, treatment influence on type response and latency during righting reflex testing. 
VPA treatment during gestation affect outcome of tail suspension test at the 5th and 10th PND (regarding the doses of 200 and 400mg/kg). There are no concequences in treated groups in tst at 15th PND. 
VPA exposition in utero distinctly influence on outcome during hot plate testing at 25th and 32nd PND. Increased procent of inadequately response and extended latency in adequate response of treated mice was obtained in hot plate test. 
Offspring which mothers were treated with VPA shown different pattern of behaviour (dose- and gender- dependent) during adolescence in elevated plus maze test (PND 35). Mice prenataly exposed to VPA during adolescence (PND40), represent significant differences during locomotor, stereotipic and vertical activity in the open field test. 
Conclusion: Experimental model of continuous application of VPA during breeding and gestation on mice is congruent with therapeutic application in epilepsy treatment during pregnancy in human population. Obtained results in this study indicate that exposure to VPA induce the effect which is dose- and gender-dependent. A subtherapeutic doses applied during gestation significantly influence on the course of psychomotor development. Methodolically, this experiments had shown that the consequences of exposition to valproate in-utero must be examined during adequate postnatal period. Mice has higher rate of VPA metabolism compared with the human rate. Regarding this fact, critical approach must be considered in approximation of the results obtained from animal model of mice to human population.
PB  - Kragujevac: University of Kragujevac, Faculty of Medical Sciences
T2  - University of Kragujevac, Faculty of Medical Sciences
T1  - Uticaj primene valproične kiseline tokom priploda i gestacije na animalnom modelu miša - efekat na psihomotorni razvoj kod potomaka
T1  - Influence of valproic acid application during breeding and gestation in the mouse animal model - effect on psychomotor development in offspring
SP  - 1
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_nardus_3741
ER  - 

@phdthesis{
author = "Podgorac, Jelena",
year = "2014",
abstract = "Osnovni cilj ove doktorske disertacije je bio da se ispitaju posledice intrauterinog izlaganja različitim dozama valproata na tok fizičkog i psihomotornog razvoja jedinki miša. Ispitivani su efekti valproata na CNS-u u subterapijskim dozama i dozno- zavisni efekat. Testovi primenjeni na potomstvu su u literaturi okarakterisani kao odgovarajući za procenu stanja motornog i senzornog sistema u određenoj fazi postnatalnog razvoja. Određeni testovi, pored opšte motorne sposobnosti, omogućavali su i procenu ponašanja tipa anksioznosti / depresije, hiper- i hipoaktivnosti jedinki.
Metode. U eksperimentima su korišćene ženke i mužjaci zdravih laboratorijskih miševa soja NMRI, odabrane metodom slučajnog izbora iz okota na Vojnomedicinskoj akademiji u Beogradu. Eksperiment je počinjao sparivanjem dve ženke i jednog mužjaka u jednom kavezu. Shodno tretmanu, oformljeno je 5 eksperimentalnih grupa i to jedna kontrolna (n=9 ženki), koja je bila tretirana fiziološkim rastvorom (Natrii Chloridi Infundibule 0,9%, HemofarmhospitalLogica), i 4 grupe koje su tretirane valproatom (Valproic acid sodium salt – 2 propylpentanoic sodium, ≥98, P4543 Sigma Aldrich) u dozi od 50 mg/kg, 100 mg/kg, 200 mg/kg ili 400 mg/kg (n=10-11 ženki po grupi). Natrijumova so valproinske kiseline rastvarana je u fiziološkom rastvoru. Injeciranje je obavljano svakodnevno, od momenta sparivanja, tokom čitavog perioda priploda i gestacije. Nakon rađanja potomstva tretman je prestajao i ženke su sa svojim potomstvom odvajane u zasebne kaveze. Pristup hrani i vodi je bio neograničen.
Procenjivan je odgovor potomstva u sledećim testovima: (1) test refleksa uspravljanja , (2)test kačenja o rep, (3) test izlaganja vrućoj ploči, (4) test uzdignutog krstastog lavirinta, (5) test otvorenog polja
Rezultati. Kontinuirana primena različitih doza valproata (50, 100, 200 i 400 mg/kg) na ženkama miša NMRI soja u periodu priploda ima negativan dozno-zavisni uticaj na fertilitet, a kontinuirana primena u periodu priploda i gestacije na smrtnost ženki (akcenat na visokim dozama). Tretman nema značajnog uticaja na telesnu težinu ženki.
Intrauterino izlaganje valproatu ima negativan uticaj na telesnu težinu jedinki tokom postnatalnog razvoja, uključujući period adolescencije. Tretman je povezan i sa kašnjenjem u otvaranju očiju jedinki u periodu do 15. dana postanatalnog razvoja.
Intrauterino izlaganje različitim dozama valproata značajno utiče na ponašanje jedinki u testu uspravljanja. Shodno starosti jedinki efekat tretmana se ogleda kako u kvalitetu (tipu) odgovora i u vremenu koje je potrebno jedinki da se uspravi.
Tretman valproatom tokom prenatalnog perioda utiče na ishod testa kačenja o rep kada se jedinke testiraju u periodu od 5. do 10. postnatalnog dana (sa akcentom na doze 200 mg/kg i 400 mg/kg). Kod jedinki starih 15 postnatalnih dana posledice tretmana nisu uočene.
Intrauterino izlaganje valproatu značajno utiče na ishod u testu izlaganja vrućoj ploči jedinki starih 25 i 32 postnatalna dana. Efekat se ogleda u povećanju broja jedinki koje neadekvatno odgovaraju na test, kao i u produženoj latenci odgovora na stimulus kod jedinki koje adekvatno odgovore na test.
Izlaganje majki tokom trudnoće valproatu značajno utiče na ponašanje adolescentnog potomstva (PND35) u testu uzdignutog krstastog lavirinta, a posledice su dozno- i polno-specifične. U testu otvorenog polja (PND40), izlaganje majki tokom trudnoće valproatu značajno utiče na ponašanje adolescentnog potomstva oba pola. Shodno primenjenoj dozi, efekat se ogleda u finom narušavanju analiziranih parametara (lokomotorna, stereotipna ili vertikalna aktivnost) u određenom periodu boravka u otvorenom polju.
Zaključak. Primenjeni eksperimentalni model kontirnuirane aplikacije valproata tokom priploda i gestacije na jedinkama miša je u korelaciji sa njegovom terapijskom primenom u tretmanu epilepsija tokom trudnoće u humanoj populaciji. Rezultati dobijeni u našim istraživanjima su jasno ukazali da posledice intrauterinog izlaganja valproatu zavise kako od primenjene doze tako i od pola potomstva. Od posebnog značaja su pokazatelji da subterapijske doze valproata primenjene tokom gestacije značajno utiču na psihomotorni razvoj jedinki. U metodološkom smislu naši eksperimenti su pokazali da za pravilno sagledavanje posledica intrauterinog izlaganja valproatu mora da postoji adekvatan opseg postnatalnih starosti jedinki. Shodno činjenici da je metabolizam valproata kod jedinki miševa ubrzan u odnosu na metabolizam ovog leka u humanoj populaciji, kritički pristup u aproksimaciji podataka dobijenih iz animalnog modela miša na ljudsku populaciju je neophodan.
Cilj ove doktorske disertacije je bio da se ispitaju posledice intrauterinog izlaganja različitim dozama valproata (sa akcentom na subterapijske doze) na psihomotorni razvoj jedinki miša, koje su testirane u različitim fazama postnatalnog razvoja. Baterija testova za ispitivanje ponašanja koja je upotrebljena tokom ove studije uključivala je sledeće testove: test refleksa uspravljanja i test kačenja o rep – tokom prve dve postnatalne nedelje, test izlaganja vrućoj ploči – u periadolescentnoj fazi, test uzdignutog krstastog lavirinta i test otvorenog polja – tokom adolescencije.
Podaci dobijeni u testu refleksa uspravljanja i testu kačenja jedinke o rep su jasno ukazali na kašnjenje u razvoju senzornog i motornog sistema jedinki koje su tokom gestacije bile izložene različitim dozama valproata, koje je uočljivo kroz kvalitativno i kvantitativno drugačiji odgovor jedinki na date testove u periodu ranog postnatalnog razvoja. Velike fizičke anomalije nisu detektovane, ali je jasno pokazan negativan uticaj intrauterinog izlaganja valproatu na telesnu težinu i otvaranje očiju. Podaci dobijeni u testu vruće ploče su dodatno ukazali na kašnjenje u sazrevanju senzornog sistema značajnog za percepciju bolnog stimulusa, sa posebnim akcentom na uticaj malih
5
doza valproata. Testovi primenjeni na jedinkama u periodu adolescencije su ukazali da intrauterino izlaganje valproatu izaziva polno- i dozno-specifične promene u ponašanju, u smislu anksiozno/depresivnih stanja kod jedinki muškog pola usled primene velikih doza valproata i hiperaktivnih stanja kod jedinki ženskog pola usled primene malih doza valproata.
Rezultati dobijeni u ovom istraživanju pružaju mogućnost sagledavanja posledica primene valproata tokom trudnoće na funkcionisanje centralnog nervnog sistema potomstva, sa aspekta ponašanja. U skladu sa literaturom, naši eksperimenti su ukazali da intrauterino izlaganje valproatu izaziva kompleksne promene u ponašanju potomstva, usled morfo-funkcionalnih poremećaja u određenim moždanim regionima. Ipak, ostaje da se precizno utvrde neuralni korelati bihejvioralnih efekata opisanih u našoj studiji koja je za model imala kontinuiranu primenu valproata tokom čitave gestacije, što je eksperimentalno za sada nedovoljno ispitano., The aim of this study was to examine the effects of intrauterine exposure to different doses of valproate on the course of physical and psychomotor development of mouse. The effects of valproate in the CNS was evaluated at subtherapeutic doses and dose-dependent effect. 
The tests applied to the offspring in the literature are labeled as suitable for the assessment of motor and sensory system at a certain stage of postnatal development. Certain tests, in addition to general motor skills, allowed the assessment of behavioral state as anxiety / depression, hyper-and hypo-activity of offspring. 
The study was done on adult female NMRI mice. Four experimental and one control group was formed. Females were treated with subcutaneous injection of 50, 100, 200 and 400 mg/kg VPA (Valproic acid sodium salt – 2 propylpentanoic sodium, ≥98, P4543 Sigma Aldrich) or with saline (Natrii Chloridi Infundibule 0,9%, HemofarmhospitalLogica) (control group). All treatments were performed during breeding and whole gestation period. Mice were given ad libitum access to food and water. The offspring was examined in tests: 
(1) Righting test, (2) Tail suspension test, (3) Hot plate test, (4) Elevated plus maze test and (5) Open field test 
Results: Different doses of VPA (50, 100, 200 and 400 mg/kg) continuously applied on female NMRI mice during breeding and whole gestation caused female mortality and lower fertility dose-dependent effect. Treatment had no influence on female body weight. 
In utero exposure to valproate has a negative impact on body weight of individuals during postnatal development, including the adolescence. Treatment is associated with a delay in eye-opening at the 15th postnatal day (PND). 
Prenatal exposition to different doses of VPA significantly influence on offspring behaviour in righting reflex. Regarding the critical developmental postnatal points, treatment influence on type response and latency during righting reflex testing. 
VPA treatment during gestation affect outcome of tail suspension test at the 5th and 10th PND (regarding the doses of 200 and 400mg/kg). There are no concequences in treated groups in tst at 15th PND. 
VPA exposition in utero distinctly influence on outcome during hot plate testing at 25th and 32nd PND. Increased procent of inadequately response and extended latency in adequate response of treated mice was obtained in hot plate test. 
Offspring which mothers were treated with VPA shown different pattern of behaviour (dose- and gender- dependent) during adolescence in elevated plus maze test (PND 35). Mice prenataly exposed to VPA during adolescence (PND40), represent significant differences during locomotor, stereotipic and vertical activity in the open field test. 
Conclusion: Experimental model of continuous application of VPA during breeding and gestation on mice is congruent with therapeutic application in epilepsy treatment during pregnancy in human population. Obtained results in this study indicate that exposure to VPA induce the effect which is dose- and gender-dependent. A subtherapeutic doses applied during gestation significantly influence on the course of psychomotor development. Methodolically, this experiments had shown that the consequences of exposition to valproate in-utero must be examined during adequate postnatal period. Mice has higher rate of VPA metabolism compared with the human rate. Regarding this fact, critical approach must be considered in approximation of the results obtained from animal model of mice to human population.",
publisher = "Kragujevac: University of Kragujevac, Faculty of Medical Sciences",
journal = "University of Kragujevac, Faculty of Medical Sciences",
title = "Uticaj primene valproične kiseline tokom priploda i gestacije na animalnom modelu miša - efekat na psihomotorni razvoj kod potomaka, Influence of valproic acid application during breeding and gestation in the mouse animal model - effect on psychomotor development in offspring",
pages = "1-133",
url = "https://hdl.handle.net/21.15107/rcub_nardus_3741"
}

Podgorac, J.. (2014). Uticaj primene valproične kiseline tokom priploda i gestacije na animalnom modelu miša - efekat na psihomotorni razvoj kod potomaka. in University of Kragujevac, Faculty of Medical Sciences
Kragujevac: University of Kragujevac, Faculty of Medical Sciences., 1-133.
https://hdl.handle.net/21.15107/rcub_nardus_3741

Podgorac J. Uticaj primene valproične kiseline tokom priploda i gestacije na animalnom modelu miša - efekat na psihomotorni razvoj kod potomaka. in University of Kragujevac, Faculty of Medical Sciences. 2014;:1-133.
https://hdl.handle.net/21.15107/rcub_nardus_3741 .

Podgorac, Jelena, "Uticaj primene valproične kiseline tokom priploda i gestacije na animalnom modelu miša - efekat na psihomotorni razvoj kod potomaka" in University of Kragujevac, Faculty of Medical Sciences (2014):1-133,
https://hdl.handle.net/21.15107/rcub_nardus_3741 .

TY  - JOUR
AU  - Sekulić, S.
AU  - Keković, G.
AU  - Filipović, Danka
AU  - Drapšin, M.
AU  - Podgorac, Jelena
AU  - Martać, Ljiljana
AU  - Novakov-Mikić, Aleksandra
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/350
AB  - The aim of this work was to determine the changes in the duration of spontaneous movements in the guinea pig fetus after the appearance of its first movements. Every day from the 25th to the 35th gestation day, one fetus from each of twenty pregnant animals was examined by ultrasound. Fetal movements were observed for 5 min. The episode with the longest period of movement was taken into consideration and was recorded as: <1 s, 1-3 s, and >3 s. Days 25 and 26 were characterized by episodes lasting <1 s; days 29 to 31 were dominated by episodes lasting 1-3 s, and days 34 and 35 by episodes lasting >3 s (χ2 = 140.51 p <0.05). Tracking the dynamics of progressive increases in the longest episode of spontaneous movement could be a useful factor in estimating the maturity and condition of a fetus.
T2  - Archives of Biological Sciences
T1  - Monitoring the progressive increase of the longest episode of spontaneous movements in Guinea pig fetus
IS  - 4
VL  - 65
SP  - 1459
EP  - 1462
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_350
ER  - 

@article{
author = "Sekulić, S. and Keković, G. and Filipović, Danka and Drapšin, M. and Podgorac, Jelena and Martać, Ljiljana and Novakov-Mikić, Aleksandra",
year = "2013, 2013",
abstract = "The aim of this work was to determine the changes in the duration of spontaneous movements in the guinea pig fetus after the appearance of its first movements. Every day from the 25th to the 35th gestation day, one fetus from each of twenty pregnant animals was examined by ultrasound. Fetal movements were observed for 5 min. The episode with the longest period of movement was taken into consideration and was recorded as: <1 s, 1-3 s, and >3 s. Days 25 and 26 were characterized by episodes lasting <1 s; days 29 to 31 were dominated by episodes lasting 1-3 s, and days 34 and 35 by episodes lasting >3 s (χ2 = 140.51 p <0.05). Tracking the dynamics of progressive increases in the longest episode of spontaneous movement could be a useful factor in estimating the maturity and condition of a fetus.",
journal = "Archives of Biological Sciences",
title = "Monitoring the progressive increase of the longest episode of spontaneous movements in Guinea pig fetus",
number = "4",
volume = "65",
pages = "1459-1462",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_350"
}

Sekulić, S., Keković, G., Filipović, D., Drapšin, M., Podgorac, J., Martać, L.,& Novakov-Mikić, A.. (2013). Monitoring the progressive increase of the longest episode of spontaneous movements in Guinea pig fetus. in Archives of Biological Sciences, 65(4), 1459-1462.
https://hdl.handle.net/21.15107/rcub_ibiss_350

Sekulić S, Keković G, Filipović D, Drapšin M, Podgorac J, Martać L, Novakov-Mikić A. Monitoring the progressive increase of the longest episode of spontaneous movements in Guinea pig fetus. in Archives of Biological Sciences. 2013;65(4):1459-1462.
https://hdl.handle.net/21.15107/rcub_ibiss_350 .

Sekulić, S., Keković, G., Filipović, Danka, Drapšin, M., Podgorac, Jelena, Martać, Ljiljana, Novakov-Mikić, Aleksandra, "Monitoring the progressive increase of the longest episode of spontaneous movements in Guinea pig fetus" in Archives of Biological Sciences, 65, no. 4 (2013):1459-1462,
https://hdl.handle.net/21.15107/rcub_ibiss_350 .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Čapo, Ivan
AU  - Sekulić, Slobodan
AU  - Pepelčević, Nenad
AU  - Martać, Ljiljana
AU  - Keković, Goran
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/516
AB  - Serotonin is, evolutionary, the oldest neurotransmitter. Ubiquitous distribution of serotonin in central nervous system, also implication in many physiological functions indicates significance of serotonergic system. Expression of the serotonin function is the most conditioned by the interaction with postsynaptic receptors. In this article, the classification of the serotonergic receptors and its role is reviewed. The expectations of future research are detection of new subpopulations of receptors, their functions and summation of knowledge in order to find new therapy and new manner in treatment of central nervous system disturbances, which imply serotonergic dysfunction.
AB  - Serotonin predstavlja jedan od evolutivno najstarijih neurotransmitrera. Njegova široka distribucija u centralnom nervnom sistemu i povezanost sa mnogobrojnim fiziološkim funkcijama ukazuje na značaj serotoninskog sistema. Karakter ispoljavanja serotonina u najvećoj meri određuje postsinaptički receptor sa kojim stupa u kontakt. U radu su prikazani do sada klasifikovani receptori i njihova uloga. Od budućih istraživanja očekuje se otkrivanje novih subpopulacija receptora, njihove uloge i sumacija saznanja u cilju pronalaženja novih lekova i načina lečenja uzrokovanih poremećajima serotinskog sistema CNS-a.
T2  - MD - Medical data
T1  - Funkcija serotoninskih receptora i njihov uticaj na ponašanje
T1  - Function of serotonin receptors and its role in the behavior
IS  - 2
VL  - 4
SP  - 167
EP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_516
ER  - 

@article{
author = "Podgorac, Jelena and Čapo, Ivan and Sekulić, Slobodan and Pepelčević, Nenad and Martać, Ljiljana and Keković, Goran",
year = "2012, 2012",
abstract = "Serotonin is, evolutionary, the oldest neurotransmitter. Ubiquitous distribution of serotonin in central nervous system, also implication in many physiological functions indicates significance of serotonergic system. Expression of the serotonin function is the most conditioned by the interaction with postsynaptic receptors. In this article, the classification of the serotonergic receptors and its role is reviewed. The expectations of future research are detection of new subpopulations of receptors, their functions and summation of knowledge in order to find new therapy and new manner in treatment of central nervous system disturbances, which imply serotonergic dysfunction., Serotonin predstavlja jedan od evolutivno najstarijih neurotransmitrera. Njegova široka distribucija u centralnom nervnom sistemu i povezanost sa mnogobrojnim fiziološkim funkcijama ukazuje na značaj serotoninskog sistema. Karakter ispoljavanja serotonina u najvećoj meri određuje postsinaptički receptor sa kojim stupa u kontakt. U radu su prikazani do sada klasifikovani receptori i njihova uloga. Od budućih istraživanja očekuje se otkrivanje novih subpopulacija receptora, njihove uloge i sumacija saznanja u cilju pronalaženja novih lekova i načina lečenja uzrokovanih poremećajima serotinskog sistema CNS-a.",
journal = "MD - Medical data",
title = "Funkcija serotoninskih receptora i njihov uticaj na ponašanje, Function of serotonin receptors and its role in the behavior",
number = "2",
volume = "4",
pages = "167-170",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_516"
}

Podgorac, J., Čapo, I., Sekulić, S., Pepelčević, N., Martać, L.,& Keković, G.. (2012). Funkcija serotoninskih receptora i njihov uticaj na ponašanje. in MD - Medical data, 4(2), 167-170.
https://hdl.handle.net/21.15107/rcub_ibiss_516

Podgorac J, Čapo I, Sekulić S, Pepelčević N, Martać L, Keković G. Funkcija serotoninskih receptora i njihov uticaj na ponašanje. in MD - Medical data. 2012;4(2):167-170.
https://hdl.handle.net/21.15107/rcub_ibiss_516 .

Podgorac, Jelena, Čapo, Ivan, Sekulić, Slobodan, Pepelčević, Nenad, Martać, Ljiljana, Keković, Goran, "Funkcija serotoninskih receptora i njihov uticaj na ponašanje" in MD - Medical data, 4, no. 2 (2012):167-170,
https://hdl.handle.net/21.15107/rcub_ibiss_516 .

TY  - JOUR
AU  - Keković, Goran
AU  - Sekulić, Slobodan R
AU  - Podgorac, Jelena
AU  - Sakac, Dejan
AU  - Marković, Jasminka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1076
AB  - In our study, we investigated the properties of electroencephalogram (EEG) of humans experiencing epileptic seizures. By applying linear methods, such as spectral analysis and wavelet transformation, we compared characteristics of EEGs of patients suffering from epilepsia with the characteristics of EEGs of healthy individuals. Our goal was to establish the real effect of epileptic attacks in frequency domains, distinguishing them from various types of artifacts caused by mental and physical activities in humans. The frequency range of 1 to 2 Hz was found to be interesting, where we have detected a power spectral density peak typical of epileptic seizures. Also, we have shown that there exists an anomalous distribution of the relative wavelet energy of the signal within this frequency range. We also showed that the frequency range 7.97 to 15.94 Hz can serve as an indicator of preictal activity in the EEG signal.
T2  - Neurophysiology
T1  - Linear Analysis of Epileptic Forms in the Electroencephalogram
IS  - 6
VL  - 44
SP  - 105
EP  - 478
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1076
ER  - 

@article{
author = "Keković, Goran and Sekulić, Slobodan R and Podgorac, Jelena and Sakac, Dejan and Marković, Jasminka",
year = "2012",
abstract = "In our study, we investigated the properties of electroencephalogram (EEG) of humans experiencing epileptic seizures. By applying linear methods, such as spectral analysis and wavelet transformation, we compared characteristics of EEGs of patients suffering from epilepsia with the characteristics of EEGs of healthy individuals. Our goal was to establish the real effect of epileptic attacks in frequency domains, distinguishing them from various types of artifacts caused by mental and physical activities in humans. The frequency range of 1 to 2 Hz was found to be interesting, where we have detected a power spectral density peak typical of epileptic seizures. Also, we have shown that there exists an anomalous distribution of the relative wavelet energy of the signal within this frequency range. We also showed that the frequency range 7.97 to 15.94 Hz can serve as an indicator of preictal activity in the EEG signal.",
journal = "Neurophysiology",
title = "Linear Analysis of Epileptic Forms in the Electroencephalogram",
number = "6",
volume = "44",
pages = "105-478",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1076"
}

Keković, G., Sekulić, S. R., Podgorac, J., Sakac, D.,& Marković, J.. (2012). Linear Analysis of Epileptic Forms in the Electroencephalogram. in Neurophysiology, 44(6), 105-478.
https://hdl.handle.net/21.15107/rcub_ibiss_1076

Keković G, Sekulić SR, Podgorac J, Sakac D, Marković J. Linear Analysis of Epileptic Forms in the Electroencephalogram. in Neurophysiology. 2012;44(6):105-478.
https://hdl.handle.net/21.15107/rcub_ibiss_1076 .

Keković, Goran, Sekulić, Slobodan R, Podgorac, Jelena, Sakac, Dejan, Marković, Jasminka, "Linear Analysis of Epileptic Forms in the Electroencephalogram" in Neurophysiology, 44, no. 6 (2012):105-478,
https://hdl.handle.net/21.15107/rcub_ibiss_1076 .

TY  - JOUR
AU  - Sekulić, Slobodan R
AU  - Podgorac, Jelena
AU  - Keković, Goran
AU  - Zarkov, Marija G
AU  - Kopitović, Aleksandar
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1086
AB  - Analysis of qualitative indicators of stability of the body during different types of locomotion in primates suggests that bipedal locomotion is not variation of some other type of locomotion. Transition from quadrupedal to bipedal locomotion is accompanied by a qualitative difference in body stability. Because of assuming an upright bipedal posture, the center of mass is lifted, the surface of the base of support is reduced, and the body structure does not provide passive stability in relation to inertial moments of the body around Y-axis. Additional head movements, trunk rotations, forelimb manipulations with objects and surveying the surroundings are necessary for survival, but they increase the degree of freedom of movement and further complicate the task of maintaining balance in the case of a postural change from erect quadrupedal to erect bipedal. This article presents a hypothesis that the transition from quadrupedal to habitual upright bipedal locomotion was caused by qualitative changes in the nervous system that allowed controlling the more demanding type of locomotion. The ability to control a more demanding posture increases possibilities of interactions between the organism and the complex environment and consequently increases the survival rate, breeding possibilities, and chances for occupying a new environmental niche. Existing data show that ability to execute the more demanding type of locomotion was made possible because of changes in the frontal lobe and pyramidal system. Only after the more demanding posture was enabled by changes in the nervous system, could advantages of bipedal over quadrupedal locomotion be utilized, including better scanning of the environment, carrying food and infants, simultaneous upper extremity movements and observation of the environment, limitless manipulation of objects with upper extremities above the individual, and less space for rotating around the Z-axis. The aforementioned advantages of habitual bipedal over quadrupedal locomotion are present in physically complex environments, such as the forest, which is associated with the appearance of habitual bipedal locomotion. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Medical Hypotheses
T1  - Significance of adequate postural control in the appearance of habitual upright bipedal locomotion
IS  - 5
VL  - 79
SP  - 287
EP  - 571
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1086
ER  - 

@article{
author = "Sekulić, Slobodan R and Podgorac, Jelena and Keković, Goran and Zarkov, Marija G and Kopitović, Aleksandar",
year = "2012",
abstract = "Analysis of qualitative indicators of stability of the body during different types of locomotion in primates suggests that bipedal locomotion is not variation of some other type of locomotion. Transition from quadrupedal to bipedal locomotion is accompanied by a qualitative difference in body stability. Because of assuming an upright bipedal posture, the center of mass is lifted, the surface of the base of support is reduced, and the body structure does not provide passive stability in relation to inertial moments of the body around Y-axis. Additional head movements, trunk rotations, forelimb manipulations with objects and surveying the surroundings are necessary for survival, but they increase the degree of freedom of movement and further complicate the task of maintaining balance in the case of a postural change from erect quadrupedal to erect bipedal. This article presents a hypothesis that the transition from quadrupedal to habitual upright bipedal locomotion was caused by qualitative changes in the nervous system that allowed controlling the more demanding type of locomotion. The ability to control a more demanding posture increases possibilities of interactions between the organism and the complex environment and consequently increases the survival rate, breeding possibilities, and chances for occupying a new environmental niche. Existing data show that ability to execute the more demanding type of locomotion was made possible because of changes in the frontal lobe and pyramidal system. Only after the more demanding posture was enabled by changes in the nervous system, could advantages of bipedal over quadrupedal locomotion be utilized, including better scanning of the environment, carrying food and infants, simultaneous upper extremity movements and observation of the environment, limitless manipulation of objects with upper extremities above the individual, and less space for rotating around the Z-axis. The aforementioned advantages of habitual bipedal over quadrupedal locomotion are present in physically complex environments, such as the forest, which is associated with the appearance of habitual bipedal locomotion. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Medical Hypotheses",
title = "Significance of adequate postural control in the appearance of habitual upright bipedal locomotion",
number = "5",
volume = "79",
pages = "287-571",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1086"
}

Sekulić, S. R., Podgorac, J., Keković, G., Zarkov, M. G.,& Kopitović, A.. (2012). Significance of adequate postural control in the appearance of habitual upright bipedal locomotion. in Medical Hypotheses, 79(5), 287-571.
https://hdl.handle.net/21.15107/rcub_ibiss_1086

Sekulić SR, Podgorac J, Keković G, Zarkov MG, Kopitović A. Significance of adequate postural control in the appearance of habitual upright bipedal locomotion. in Medical Hypotheses. 2012;79(5):287-571.
https://hdl.handle.net/21.15107/rcub_ibiss_1086 .

Sekulić, Slobodan R, Podgorac, Jelena, Keković, Goran, Zarkov, Marija G, Kopitović, Aleksandar, "Significance of adequate postural control in the appearance of habitual upright bipedal locomotion" in Medical Hypotheses, 79, no. 5 (2012):287-571,
https://hdl.handle.net/21.15107/rcub_ibiss_1086 .

TY  - JOUR
AU  - Sekulić, Slobodan R
AU  - Bozić, Aleksandar K
AU  - Zarkov, Marija G
AU  - Keković, Goran
AU  - Podgorac, Jelena
AU  - Novakov-Mikić, Aleksandra S
AU  - Martać, Ljiljana
AU  - Barna, Tomislav
AU  - Milovanović, Aleksandar
AU  - Pusić, Ivan
AU  - Stojanović, Dragica
AU  - Pepelcević, Nenad
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1217
AB  - The hypothesis tested was that fetal postural development causes anterior presentation of the sheep fetus during a period of exclusive increase in anterior presentation. Exposing fetuses to a ventro-sacral position during a period of equal incidence of anterior and posterior presentations [50th-90th gestation day (GD)] should not cause changes in fetal presentation. During a period of an exclusive increase in anterior presentation (90th-140th GD) a ventro-sacral position should cause a 1800 turn of the fetus. Singleton gestations with fetus in anterior presentation were included. Ultrasound examinations consisted of three consecutive two minute scans to determine fetal presentation, and were conducted with the ewe in the following three positions: the upright quadrupedal position, sitting position in which the ewe's trunk is vertical to the ground; and again the upright quadrupedal position. The Mann-Whitney nonparametric U-test was used. On the 83rd GD (14 fetuses), there were no changes in fetal presentation, whereas on the 86th GD (10 fetuses) (p=0.035 p<0.05) and on the 116th GD (12 fetuses) (p=0.000 p<0.05) fetuses frequently assumed a transverse presentation at the second examination position. Further experiments would require the fetuses to be artificially maintained in a ventro-sacral position for a prolonged period.
T2  - Philippine Journal of Veterinary Medicine
T1  - Changes in the Anterior Presentation in Sheep Fetuses Due to Their Ventro-Sacral Position in the Second Half of Gestation
IS  - 1
VL  - 49
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1217
ER  - 

@article{
author = "Sekulić, Slobodan R and Bozić, Aleksandar K and Zarkov, Marija G and Keković, Goran and Podgorac, Jelena and Novakov-Mikić, Aleksandra S and Martać, Ljiljana and Barna, Tomislav and Milovanović, Aleksandar and Pusić, Ivan and Stojanović, Dragica and Pepelcević, Nenad",
year = "2012",
abstract = "The hypothesis tested was that fetal postural development causes anterior presentation of the sheep fetus during a period of exclusive increase in anterior presentation. Exposing fetuses to a ventro-sacral position during a period of equal incidence of anterior and posterior presentations [50th-90th gestation day (GD)] should not cause changes in fetal presentation. During a period of an exclusive increase in anterior presentation (90th-140th GD) a ventro-sacral position should cause a 1800 turn of the fetus. Singleton gestations with fetus in anterior presentation were included. Ultrasound examinations consisted of three consecutive two minute scans to determine fetal presentation, and were conducted with the ewe in the following three positions: the upright quadrupedal position, sitting position in which the ewe's trunk is vertical to the ground; and again the upright quadrupedal position. The Mann-Whitney nonparametric U-test was used. On the 83rd GD (14 fetuses), there were no changes in fetal presentation, whereas on the 86th GD (10 fetuses) (p=0.035 p<0.05) and on the 116th GD (12 fetuses) (p=0.000 p<0.05) fetuses frequently assumed a transverse presentation at the second examination position. Further experiments would require the fetuses to be artificially maintained in a ventro-sacral position for a prolonged period.",
journal = "Philippine Journal of Veterinary Medicine",
title = "Changes in the Anterior Presentation in Sheep Fetuses Due to Their Ventro-Sacral Position in the Second Half of Gestation",
number = "1",
volume = "49",
pages = "56",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1217"
}

Sekulić, S. R., Bozić, A. K., Zarkov, M. G., Keković, G., Podgorac, J., Novakov-Mikić, A. S., Martać, L., Barna, T., Milovanović, A., Pusić, I., Stojanović, D.,& Pepelcević, N.. (2012). Changes in the Anterior Presentation in Sheep Fetuses Due to Their Ventro-Sacral Position in the Second Half of Gestation. in Philippine Journal of Veterinary Medicine, 49(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1217

Sekulić SR, Bozić AK, Zarkov MG, Keković G, Podgorac J, Novakov-Mikić AS, Martać L, Barna T, Milovanović A, Pusić I, Stojanović D, Pepelcević N. Changes in the Anterior Presentation in Sheep Fetuses Due to Their Ventro-Sacral Position in the Second Half of Gestation. in Philippine Journal of Veterinary Medicine. 2012;49(1):null-56.
https://hdl.handle.net/21.15107/rcub_ibiss_1217 .

Sekulić, Slobodan R, Bozić, Aleksandar K, Zarkov, Marija G, Keković, Goran, Podgorac, Jelena, Novakov-Mikić, Aleksandra S, Martać, Ljiljana, Barna, Tomislav, Milovanović, Aleksandar, Pusić, Ivan, Stojanović, Dragica, Pepelcević, Nenad, "Changes in the Anterior Presentation in Sheep Fetuses Due to Their Ventro-Sacral Position in the Second Half of Gestation" in Philippine Journal of Veterinary Medicine, 49, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1217 .

TY  - JOUR
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Sekulić, S.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/261
AB  - Our previous investigations on an animal model of neurotoxicity show that increased power in the delta range is connected with the neurotoxic effect of aluminum exposure. In this study we used several aluminum-treated animals as a reliable model for the evaluation of the neurotoxic effects of aluminum on neurons, and compared it with a control group. We conclude that spectral analysis and the ratio between the delta and theta ranges might be reliable for a qualitative description of the neurotoxic effect of aluminum, and that the t test might be used to evaluate the change in brain activity between the treated and control groups of animals. The animal model under anesthesia was used to describe the state of brain activity with neurotoxicity with suppressed functional connectivity in the brain structure. We also performed fractal analysis to quantitatively describe neurotoxic effect in different pathophysiological states of animals treated with different doses of aluminum. A decrease in the fractal dimension is an indicator of neurodegeneration in the state of stress. This animal model is suitable for evaluation of the neurodegenerative processes in Alzheimer's dementia and Parkinson's disease.
AB  - Prethodna istraživanja na animalnom modelu neurotoksičnosti aluminijuma pokazuju da povećanje spektralne snage u delta opsegu je indikator neurotoksičnosti. U ovoj studiji smo koristili nekoliko životinja koje su pogodne za procenu neurotoksičnog efekta aluminijuma u poređenju sa kontrolom. Za kvalitativni opis neurotoksičnosti koristili smo odnos delta i teta opsega i t test za opis promene između tretiranih i kontrolnih pacova. Korišćen je animalni model u uslovima anestezije je opis moždane aktivnosti sa suprimiranim funkcionalnim vezama između moždanih struktura. Koristili smo i fraktalnu dimenziju za kvantitativni opis neurotoksičnog efekta u različitim patofiziološkim stanjima. Smanjenje fraktalne dimenzije je izraz neurodegeneracije u uslovima stresa. Ovaj model je komparabilan sa neurodegenerativnim i neurotransmiterskim promenama u Alchejmerovoj bolesti i Parkinsonovoj bolesti.
T2  - Archives of Biological Sciences
T1  - Procena neurotoksičnog efekta aluminijuma u animalnom modelu Wistar pacova
T1  - Evaluation of the neurotoxical effect of aluminum on the Wistar rat
IS  - 3
VL  - 62
SP  - 585
EP  - 588
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_261
ER  - 

@article{
author = "Martać, Ljiljana and Podgorac, Jelena and Sekulić, S.",
year = "2010, 2010",
abstract = "Our previous investigations on an animal model of neurotoxicity show that increased power in the delta range is connected with the neurotoxic effect of aluminum exposure. In this study we used several aluminum-treated animals as a reliable model for the evaluation of the neurotoxic effects of aluminum on neurons, and compared it with a control group. We conclude that spectral analysis and the ratio between the delta and theta ranges might be reliable for a qualitative description of the neurotoxic effect of aluminum, and that the t test might be used to evaluate the change in brain activity between the treated and control groups of animals. The animal model under anesthesia was used to describe the state of brain activity with neurotoxicity with suppressed functional connectivity in the brain structure. We also performed fractal analysis to quantitatively describe neurotoxic effect in different pathophysiological states of animals treated with different doses of aluminum. A decrease in the fractal dimension is an indicator of neurodegeneration in the state of stress. This animal model is suitable for evaluation of the neurodegenerative processes in Alzheimer's dementia and Parkinson's disease., Prethodna istraživanja na animalnom modelu neurotoksičnosti aluminijuma pokazuju da povećanje spektralne snage u delta opsegu je indikator neurotoksičnosti. U ovoj studiji smo koristili nekoliko životinja koje su pogodne za procenu neurotoksičnog efekta aluminijuma u poređenju sa kontrolom. Za kvalitativni opis neurotoksičnosti koristili smo odnos delta i teta opsega i t test za opis promene između tretiranih i kontrolnih pacova. Korišćen je animalni model u uslovima anestezije je opis moždane aktivnosti sa suprimiranim funkcionalnim vezama između moždanih struktura. Koristili smo i fraktalnu dimenziju za kvantitativni opis neurotoksičnog efekta u različitim patofiziološkim stanjima. Smanjenje fraktalne dimenzije je izraz neurodegeneracije u uslovima stresa. Ovaj model je komparabilan sa neurodegenerativnim i neurotransmiterskim promenama u Alchejmerovoj bolesti i Parkinsonovoj bolesti.",
journal = "Archives of Biological Sciences",
title = "Procena neurotoksičnog efekta aluminijuma u animalnom modelu Wistar pacova, Evaluation of the neurotoxical effect of aluminum on the Wistar rat",
number = "3",
volume = "62",
pages = "585-588",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_261"
}

Martać, L., Podgorac, J.,& Sekulić, S.. (2010). Procena neurotoksičnog efekta aluminijuma u animalnom modelu Wistar pacova. in Archives of Biological Sciences, 62(3), 585-588.
https://hdl.handle.net/21.15107/rcub_ibiss_261

Martać L, Podgorac J, Sekulić S. Procena neurotoksičnog efekta aluminijuma u animalnom modelu Wistar pacova. in Archives of Biological Sciences. 2010;62(3):585-588.
https://hdl.handle.net/21.15107/rcub_ibiss_261 .

Martać, Ljiljana, Podgorac, Jelena, Sekulić, S., "Procena neurotoksičnog efekta aluminijuma u animalnom modelu Wistar pacova" in Archives of Biological Sciences, 62, no. 3 (2010):585-588,
https://hdl.handle.net/21.15107/rcub_ibiss_261 .

TY  - JOUR
AU  - Martać, Ljiljana
AU  - Stojadinović, Gordana
AU  - Keković, G.
AU  - Podgorac, Jelena
AU  - Ćulić, Milka
AU  - Sekulić, S.
AU  - Lalošević, D.
AU  - Čapo, I.
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/243
AB  - Exposure to aluminium during gestation causes changes in mammalian brain development and behavior. Our aim was to perform spectral analysis on electrocortical activity of Sprague Dawley male pups (30±3 days of age) whose mothers were treated with aluminium during gestation and lactation. There was a higher presence of power spectra in the delta range of parietal electrocortical activity, a lower presence in the theta range and increased values of the parameter DT as the ratio of delta to theta range in pups indirectly exposed to aluminium (whose mothers were drinking a 0.5% water solution of aluminium chloride during the gestation and lactation periods), compared to controls.
AB  - Izlaganje dejstvu aluminijuma tokom gestacije i laktacije može dovesti do promena u razviću mozga i ponašanja kod sisara. Naš cilj je bio da se uradi spektralna analiza elektrokortikalne aktivnosti mladih Sprague Dawley pacova muškog pola (starosti 30±3 dana) čije su majke bile tretirane aluminijumom tokom gestacije i laktacije. U poređenju sa kontrolom, kod mladih pacova koji su bili indirektno trovani aluminijumom (čije su majke pile 0,5 % vodeni rastvor AlCl3 tokom gestacionog i laktacionog perioda) postoji veća zastupljenost delta ritma aktivnosti kore velikog mozga, manje prisustvo teta ritma, kao i porast parametra DT kao odnos između delta i teta opsega.
T2  - Archives of Biological Sciences
T1  - Spektralne promene moždane aktivnosti kod mladunaca pacova izloženih dejstvu aluminijuma tokom gestacije i laktacije
T1  - Spectral changes of brain activity in rat offspring exposed to aluminium during gestation and lactation
IS  - 1
VL  - 62
SP  - 9
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_243
ER  - 

@article{
author = "Martać, Ljiljana and Stojadinović, Gordana and Keković, G. and Podgorac, Jelena and Ćulić, Milka and Sekulić, S. and Lalošević, D. and Čapo, I.",
year = "2010, 2010",
abstract = "Exposure to aluminium during gestation causes changes in mammalian brain development and behavior. Our aim was to perform spectral analysis on electrocortical activity of Sprague Dawley male pups (30±3 days of age) whose mothers were treated with aluminium during gestation and lactation. There was a higher presence of power spectra in the delta range of parietal electrocortical activity, a lower presence in the theta range and increased values of the parameter DT as the ratio of delta to theta range in pups indirectly exposed to aluminium (whose mothers were drinking a 0.5% water solution of aluminium chloride during the gestation and lactation periods), compared to controls., Izlaganje dejstvu aluminijuma tokom gestacije i laktacije može dovesti do promena u razviću mozga i ponašanja kod sisara. Naš cilj je bio da se uradi spektralna analiza elektrokortikalne aktivnosti mladih Sprague Dawley pacova muškog pola (starosti 30±3 dana) čije su majke bile tretirane aluminijumom tokom gestacije i laktacije. U poređenju sa kontrolom, kod mladih pacova koji su bili indirektno trovani aluminijumom (čije su majke pile 0,5 % vodeni rastvor AlCl3 tokom gestacionog i laktacionog perioda) postoji veća zastupljenost delta ritma aktivnosti kore velikog mozga, manje prisustvo teta ritma, kao i porast parametra DT kao odnos između delta i teta opsega.",
journal = "Archives of Biological Sciences",
title = "Spektralne promene moždane aktivnosti kod mladunaca pacova izloženih dejstvu aluminijuma tokom gestacije i laktacije, Spectral changes of brain activity in rat offspring exposed to aluminium during gestation and lactation",
number = "1",
volume = "62",
pages = "9-13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_243"
}

Martać, L., Stojadinović, G., Keković, G., Podgorac, J., Ćulić, M., Sekulić, S., Lalošević, D.,& Čapo, I.. (2010). Spektralne promene moždane aktivnosti kod mladunaca pacova izloženih dejstvu aluminijuma tokom gestacije i laktacije. in Archives of Biological Sciences, 62(1), 9-13.
https://hdl.handle.net/21.15107/rcub_ibiss_243

Martać L, Stojadinović G, Keković G, Podgorac J, Ćulić M, Sekulić S, Lalošević D, Čapo I. Spektralne promene moždane aktivnosti kod mladunaca pacova izloženih dejstvu aluminijuma tokom gestacije i laktacije. in Archives of Biological Sciences. 2010;62(1):9-13.
https://hdl.handle.net/21.15107/rcub_ibiss_243 .

Martać, Ljiljana, Stojadinović, Gordana, Keković, G., Podgorac, Jelena, Ćulić, Milka, Sekulić, S., Lalošević, D., Čapo, I., "Spektralne promene moždane aktivnosti kod mladunaca pacova izloženih dejstvu aluminijuma tokom gestacije i laktacije" in Archives of Biological Sciences, 62, no. 1 (2010):9-13,
https://hdl.handle.net/21.15107/rcub_ibiss_243 .

TY  - JOUR
AU  - Keković, Goran
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Podgorac, Jelena
AU  - Sekulić, Slobodan R
AU  - Ćulić, Milka
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1127
AB  - The features of rat cerebral and cerebellar electrocortical activity (ECoG) under different types of anaesthesia (nembutal. ketamine or zoletil) were examined by the distribution of spectral entropy across frequency bands of ECoG and by calculation of fractal dimension determined on the basis of Higuchi's algorithm. Spectral entropy, as a measure of activity, in the case of cerebrum had greater values than the spectral entropy of cerebellum in low frequency ranges, regardless of the type or applied anesthetic. Various anesthetics evoked different effects on spectral entropy of electrocortical activity: spectral entropy of delta range greatly dominated under nembutal anesthesia, while ketamine or zoletil appeared to affect the spectral entropy of higher frequency ranges. The pronounced effect of ketamine or zoletil anesthesia on spectral entropy of higher frequency was confirmed by the higher values of Higucihi's fractal dimension (ED) of ECoGs, with a tendency of higher ED values in cerebellar activity than cerebral activity.
T2  - Acta Neurobiologiae Experimentalis
T1  - Spectral and fractal measures of cerebellar and cerebral activity in various types of anesthesia
IS  - 1
VL  - 70
SP  - 173
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1127
ER  - 

@article{
author = "Keković, Goran and Stojadinović, Gordana and Martać, Ljiljana and Podgorac, Jelena and Sekulić, Slobodan R and Ćulić, Milka",
year = "2010",
abstract = "The features of rat cerebral and cerebellar electrocortical activity (ECoG) under different types of anaesthesia (nembutal. ketamine or zoletil) were examined by the distribution of spectral entropy across frequency bands of ECoG and by calculation of fractal dimension determined on the basis of Higuchi's algorithm. Spectral entropy, as a measure of activity, in the case of cerebrum had greater values than the spectral entropy of cerebellum in low frequency ranges, regardless of the type or applied anesthetic. Various anesthetics evoked different effects on spectral entropy of electrocortical activity: spectral entropy of delta range greatly dominated under nembutal anesthesia, while ketamine or zoletil appeared to affect the spectral entropy of higher frequency ranges. The pronounced effect of ketamine or zoletil anesthesia on spectral entropy of higher frequency was confirmed by the higher values of Higucihi's fractal dimension (ED) of ECoGs, with a tendency of higher ED values in cerebellar activity than cerebral activity.",
journal = "Acta Neurobiologiae Experimentalis",
title = "Spectral and fractal measures of cerebellar and cerebral activity in various types of anesthesia",
number = "1",
volume = "70",
pages = "173-75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1127"
}

Keković, G., Stojadinović, G., Martać, L., Podgorac, J., Sekulić, S. R.,& Ćulić, M.. (2010). Spectral and fractal measures of cerebellar and cerebral activity in various types of anesthesia. in Acta Neurobiologiae Experimentalis, 70(1), 173-75.
https://hdl.handle.net/21.15107/rcub_ibiss_1127

Keković G, Stojadinović G, Martać L, Podgorac J, Sekulić SR, Ćulić M. Spectral and fractal measures of cerebellar and cerebral activity in various types of anesthesia. in Acta Neurobiologiae Experimentalis. 2010;70(1):173-75.
https://hdl.handle.net/21.15107/rcub_ibiss_1127 .

Keković, Goran, Stojadinović, Gordana, Martać, Ljiljana, Podgorac, Jelena, Sekulić, Slobodan R, Ćulić, Milka, "Spectral and fractal measures of cerebellar and cerebral activity in various types of anesthesia" in Acta Neurobiologiae Experimentalis, 70, no. 1 (2010):173-75,
https://hdl.handle.net/21.15107/rcub_ibiss_1127 .

TY  - JOUR
AU  - Ćulić, Milka
AU  - Keković, Goran
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Soković, Marina
AU  - Podgorac, Jelena
AU  - Sekulić, Slobodan R
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1133
AB  - We investigated the rat brain activity in acute seizures evoked by camphor essential oil or its main constituent 1,8-cineole by wavelet (primarily) and fractal analysis. Experiments were performed on anesthetized animals before and after intraperitoneal camphor oil or cineole administration. The properties of frequency bands in pre-ictal, ictal and inter-ictal stages have been determined by wavelet analysis. The domination of 6 frequency band was confirmed in obtained brain activities, which participate with approximate to 45% of mean relative wavelet energy (MRWE) in control signals and arise up to approximate to 76% MRWE in energy spectrum during the ictal stage (after drug administration). Other frequency bands decreased during ictal stage and arised in inter-ictal stage. There was a dose-dependent response of cineole effect: increase in cineole concentration leaded to the higher values of relative wavelet energy (RWE) of delta frequency band while there were slight changes of the mean fractal dimension (FD) values as a measure of system complexity.
T2  - General Physiology and Biophysics
T1  - Wavelet and fractal analysis of rat brain activity in seizures evoked by camphor essential oil and 1,8-cineole
IS  - null
VL  - 28
SP  - 289
EP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1133
ER  - 

@article{
author = "Ćulić, Milka and Keković, Goran and Stojadinović, Gordana and Martać, Ljiljana and Soković, Marina and Podgorac, Jelena and Sekulić, Slobodan R",
year = "2009",
abstract = "We investigated the rat brain activity in acute seizures evoked by camphor essential oil or its main constituent 1,8-cineole by wavelet (primarily) and fractal analysis. Experiments were performed on anesthetized animals before and after intraperitoneal camphor oil or cineole administration. The properties of frequency bands in pre-ictal, ictal and inter-ictal stages have been determined by wavelet analysis. The domination of 6 frequency band was confirmed in obtained brain activities, which participate with approximate to 45% of mean relative wavelet energy (MRWE) in control signals and arise up to approximate to 76% MRWE in energy spectrum during the ictal stage (after drug administration). Other frequency bands decreased during ictal stage and arised in inter-ictal stage. There was a dose-dependent response of cineole effect: increase in cineole concentration leaded to the higher values of relative wavelet energy (RWE) of delta frequency band while there were slight changes of the mean fractal dimension (FD) values as a measure of system complexity.",
journal = "General Physiology and Biophysics",
title = "Wavelet and fractal analysis of rat brain activity in seizures evoked by camphor essential oil and 1,8-cineole",
number = "null",
volume = "28",
pages = "289-40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1133"
}

Ćulić, M., Keković, G., Stojadinović, G., Martać, L., Soković, M., Podgorac, J.,& Sekulić, S. R.. (2009). Wavelet and fractal analysis of rat brain activity in seizures evoked by camphor essential oil and 1,8-cineole. in General Physiology and Biophysics, 28(null), 289-40.
https://hdl.handle.net/21.15107/rcub_ibiss_1133

Ćulić M, Keković G, Stojadinović G, Martać L, Soković M, Podgorac J, Sekulić SR. Wavelet and fractal analysis of rat brain activity in seizures evoked by camphor essential oil and 1,8-cineole. in General Physiology and Biophysics. 2009;28(null):289-40.
https://hdl.handle.net/21.15107/rcub_ibiss_1133 .

Ćulić, Milka, Keković, Goran, Stojadinović, Gordana, Martać, Ljiljana, Soković, Marina, Podgorac, Jelena, Sekulić, Slobodan R, "Wavelet and fractal analysis of rat brain activity in seizures evoked by camphor essential oil and 1,8-cineole" in General Physiology and Biophysics, 28, no. null (2009):289-40,
https://hdl.handle.net/21.15107/rcub_ibiss_1133 .