TY  - JOUR
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Korać, Aleksandra
AU  - Ćirović, Duško
AU  - Otašević, Vesna
AU  - Storey, Kenneth B.
AU  - Korać, Bato
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1096495918300198
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3011
AB  - In the present study we hypothesized that myocardial adaptive phenotype in mammalian hibernation involves rearrangement of mitochondria bioenergetic pathways providing protective pattern in states of reduced metabolism and low temperature. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 ± 1 °C) and then divided into two groups: (1) animals that fell into torpor (hibernating group) and (2) animals that stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). Protein levels of selected components of the electron transport chain and ATP synthase in the heart increased after prolonged cold acclimation (mainly from day 7-21 of cold exposure) and during hibernation. Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) was also upregulated under both cold exposure and hibernating conditions. The phosphorylation state (Thr172) of 5'-AMP-activated protein kinase α increased early in cold exposure (at day 1 and 3) along with increased protein levels of phosphofructokinase and pyruvate dehydrogenase, whereas hypoxia inducible factor 1α protein levels showed no changes in response to cold exposure or hibernation. Hibernation also resulted in protein upregulation of three antioxidant defense enzymes (manganese and copper/zinc superoxide dismutases and glutathione peroxidase) and thioredoxin in the heart. Cold-exposed and hibernation-related phenotypes of the heart are characterized by improved molecular basis for mitochondrial energy-producing and antioxidant capacities that are achieved in a controlled manner. The recapitulation of such adaptive mechanisms found in hibernators could have broad application for myocardial protection from ishemia/reperfusion to improve hypothermic survival and cold preservation of hearts from non-hibernating species, including humans.
T2  - Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology
T1  - A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection.
VL  - 219-220
DO  - 10.1016/j.cbpb.2018.02.004
SP  - 1
EP  - 9
ER  - 

@article{
author = "Stančić, Ana and Janković, Aleksandra and Korać, Aleksandra and Ćirović, Duško and Otašević, Vesna and Storey, Kenneth B. and Korać, Bato",
year = "2018",
abstract = "In the present study we hypothesized that myocardial adaptive phenotype in mammalian hibernation involves rearrangement of mitochondria bioenergetic pathways providing protective pattern in states of reduced metabolism and low temperature. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 ± 1 °C) and then divided into two groups: (1) animals that fell into torpor (hibernating group) and (2) animals that stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). Protein levels of selected components of the electron transport chain and ATP synthase in the heart increased after prolonged cold acclimation (mainly from day 7-21 of cold exposure) and during hibernation. Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) was also upregulated under both cold exposure and hibernating conditions. The phosphorylation state (Thr172) of 5'-AMP-activated protein kinase α increased early in cold exposure (at day 1 and 3) along with increased protein levels of phosphofructokinase and pyruvate dehydrogenase, whereas hypoxia inducible factor 1α protein levels showed no changes in response to cold exposure or hibernation. Hibernation also resulted in protein upregulation of three antioxidant defense enzymes (manganese and copper/zinc superoxide dismutases and glutathione peroxidase) and thioredoxin in the heart. Cold-exposed and hibernation-related phenotypes of the heart are characterized by improved molecular basis for mitochondrial energy-producing and antioxidant capacities that are achieved in a controlled manner. The recapitulation of such adaptive mechanisms found in hibernators could have broad application for myocardial protection from ishemia/reperfusion to improve hypothermic survival and cold preservation of hearts from non-hibernating species, including humans.",
journal = "Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology",
title = "A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection.",
volume = "219-220",
doi = "10.1016/j.cbpb.2018.02.004",
pages = "1-9"
}

Stančić, A., Janković, A., Korać, A., Ćirović, D., Otašević, V., Storey, K. B.,& Korać, B.. (2018). A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection.. in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology, 219-220, 1-9.
https://doi.org/10.1016/j.cbpb.2018.02.004

Stančić A, Janković A, Korać A, Ćirović D, Otašević V, Storey KB, Korać B. A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection.. in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology. 2018;219-220:1-9.
doi:10.1016/j.cbpb.2018.02.004 .

Stančić, Ana, Janković, Aleksandra, Korać, Aleksandra, Ćirović, Duško, Otašević, Vesna, Storey, Kenneth B., Korać, Bato, "A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection." in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology, 219-220 (2018):1-9,
https://doi.org/10.1016/j.cbpb.2018.02.004 . .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Storey, Kenneth B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/944
AB  - Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Free Radical Biology and Medicine
T1  - The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update
IS  - null
VL  - 65
SP  - 47
EP  - 924
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_944
ER  - 

@article{
author = "Vučetić, Milica and Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Storey, Kenneth B and Korać, Bato",
year = "2013",
abstract = "Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Free Radical Biology and Medicine",
title = "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update",
number = "null",
volume = "65",
pages = "47-924",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_944"
}

Vučetić, M., Stančić, A., Otašević, V., Janković, A., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J., Storey, K. B.,& Korać, B.. (2013). The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine, 65(null), 47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944

Vučetić M, Stančić A, Otašević V, Janković A, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Storey KB, Korać B. The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine. 2013;65(null):47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

Vučetić, Milica, Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Storey, Kenneth B, Korać, Bato, "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update" in Free Radical Biology and Medicine, 65, no. null (2013):47-924,
https://hdl.handle.net/21.15107/rcub_ibiss_944 .