TY  - JOUR
AU  - Janković, Aleksandra
AU  - Zakić, Tamara
AU  - Miličić, Miroslav
AU  - Unić-Stojanović, Dragana
AU  - Kalezić, Anđelika
AU  - Korać, Aleksandra
AU  - Jović, Miomir
AU  - Korać, Bato
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/12/1910
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8750270
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4740
AB  - Remote ischaemic preconditioning (RIPC) is a medical procedure that consists of repeated brief periods of transient ischaemia and reperfusion of distant organs (limbs) with the ability to provide internal organ protection from ischaemia. Even though RIPC has been successfully applied in patients with myocardial infarction during coronary revascularization (surgery/percutaneous angioplasty), the underlying molecular mechanisms are yet to be clarified. Thus, our study aimed to determine the role of nitric oxide synthase (NOS) isoforms in RIPC-induced protection (3 × 5 min of forearm ischaemia with 5 min of reperfusion) of arterial graft in patients undergoing urgent coronary artery bypass grafting (CABG). We examined RIPC effects on specific expression and immunolocalization of three NOS isoforms - endothelial (eNOS), inducible (iNOS) and neuronal (nNOS) in patients' internal thoracic artery (ITA) used as a graft. We found that the application of RIPC protocol leads to an increased protein expression of eNOS, which was further confirmed with strong eNOS immunopositivity, especially in the endothelium and smooth muscle cells of ITA. The same analysis of two other NOS isoforms, iNOS and nNOS, showed no significant differences between patients undergoing CABG with or without RIPC. Our results demonstrate RIPC-induced upregulation of eNOS in human ITA, pointing to its significance in achieving protective phenotype on a systemic level with important implications for graft patency.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting.
IS  - 12
VL  - 10
DO  - 10.3390/antiox10121910
SP  - 1910
ER  - 

@article{
author = "Janković, Aleksandra and Zakić, Tamara and Miličić, Miroslav and Unić-Stojanović, Dragana and Kalezić, Anđelika and Korać, Aleksandra and Jović, Miomir and Korać, Bato",
year = "2021",
abstract = "Remote ischaemic preconditioning (RIPC) is a medical procedure that consists of repeated brief periods of transient ischaemia and reperfusion of distant organs (limbs) with the ability to provide internal organ protection from ischaemia. Even though RIPC has been successfully applied in patients with myocardial infarction during coronary revascularization (surgery/percutaneous angioplasty), the underlying molecular mechanisms are yet to be clarified. Thus, our study aimed to determine the role of nitric oxide synthase (NOS) isoforms in RIPC-induced protection (3 × 5 min of forearm ischaemia with 5 min of reperfusion) of arterial graft in patients undergoing urgent coronary artery bypass grafting (CABG). We examined RIPC effects on specific expression and immunolocalization of three NOS isoforms - endothelial (eNOS), inducible (iNOS) and neuronal (nNOS) in patients' internal thoracic artery (ITA) used as a graft. We found that the application of RIPC protocol leads to an increased protein expression of eNOS, which was further confirmed with strong eNOS immunopositivity, especially in the endothelium and smooth muscle cells of ITA. The same analysis of two other NOS isoforms, iNOS and nNOS, showed no significant differences between patients undergoing CABG with or without RIPC. Our results demonstrate RIPC-induced upregulation of eNOS in human ITA, pointing to its significance in achieving protective phenotype on a systemic level with important implications for graft patency.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting.",
number = "12",
volume = "10",
doi = "10.3390/antiox10121910",
pages = "1910"
}

Janković, A., Zakić, T., Miličić, M., Unić-Stojanović, D., Kalezić, A., Korać, A., Jović, M.,& Korać, B.. (2021). Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 10(12), 1910.
https://doi.org/10.3390/antiox10121910

Janković A, Zakić T, Miličić M, Unić-Stojanović D, Kalezić A, Korać A, Jović M, Korać B. Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting.. in Antioxidants (Basel, Switzerland). 2021;10(12):1910.
doi:10.3390/antiox10121910 .

Janković, Aleksandra, Zakić, Tamara, Miličić, Miroslav, Unić-Stojanović, Dragana, Kalezić, Anđelika, Korać, Aleksandra, Jović, Miomir, Korać, Bato, "Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting." in Antioxidants (Basel, Switzerland), 10, no. 12 (2021):1910,
https://doi.org/10.3390/antiox10121910 . .

TY  - JOUR
AU  - Jović, Miomir Đ
AU  - Stančić, Ana
AU  - Nenadić, Dragan
AU  - Cekić, Olivera
AU  - Nezić, Dusko G
AU  - Milojević, Predrag S
AU  - Micović, Slobodan V
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1138
AB  - Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel
T2  - Cellular Physiology and Biochemistry
T1  - Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass
IS  - 1-2
VL  - 29
SP  - 973
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1138
ER  - 

@article{
author = "Jović, Miomir Đ and Stančić, Ana and Nenadić, Dragan and Cekić, Olivera and Nezić, Dusko G and Milojević, Predrag S and Micović, Slobodan V and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2012",
abstract = "Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel",
journal = "Cellular Physiology and Biochemistry",
title = "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass",
number = "1-2",
volume = "29",
pages = "973-142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1138"
}

Jović, M. Đ., Stančić, A., Nenadić, D., Cekić, O., Nezić, D. G., Milojević, P. S., Micović, S. V., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Velicković, K. D., Golić, I.,& Korać, B.. (2012). Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry, 29(1-2), 973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138

Jović MĐ, Stančić A, Nenadić D, Cekić O, Nezić DG, Milojević PS, Micović SV, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Velicković KD, Golić I, Korać B. Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry. 2012;29(1-2):973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

Jović, Miomir Đ, Stančić, Ana, Nenadić, Dragan, Cekić, Olivera, Nezić, Dusko G, Milojević, Predrag S, Micović, Slobodan V, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass" in Cellular Physiology and Biochemistry, 29, no. 1-2 (2012):973-142,
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .