TY  - JOUR
AU  - Otašević, Vesna
AU  - Kalezić, Anđelika
AU  - Macanović, Biljana
AU  - Janković, Aleksandra
AU  - Stančić, Ana
AU  - Garalejić, Eliana
AU  - Korać, Aleksandra
AU  - Korać, Bato
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/19396368.2019.1600171
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3330
AB  - Protein expression/activity of antioxidative defense enzymes (AD) in seminal plasma of fertile men might be used as biomarkers of male fertility status. To test this concept, the present study examined the semen parameters of males among 14 normal idiopathic (normozoospermia) and 84 subnormal (teratozoospermia, oligoteratozoospermia, oligoasthenoteratozoospermia) infertile individuals\. We investigated levels of protein expression/activity of Cu, Zn superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GSH-Px), their association with functional sperm parameters, as well as their potential to serve as biomarkers of specific sperm pathologies. Although the activity of CuZnSOD and protein expression of catalase were significantly correlated with several sperm parameters, underlying their potential role in etiology of various sperm abnormalities, investigation of their potential usefulness as a biomarker of semen quality showed that these AD enzymes could not distinguish subtle differences between various sperm pathologies. In contrast, GSH-Px activity was decreased in all groups with sperm pathologies and was a very good indicator of aberrations in functional sperm parameters, explaining up to 94.6% of infertility cases where functional sperm parameters were affected. Therefore, assessment of GSH-Px activity showed the potential to discriminate between infertile males with normal and subnormal semen characteristics and may prove useful in the evaluation of male (in)fertility. Abbreviations: AD: antioxidative defense; Cu, Zn SOD: copper, zinc superoxide dismutase; GSH-Px: glutathione peroxidase; MnSOD: manganese superoxide dismutase; NS: normospermia; OATS: oligoasthenoteratozoospermia; OTS: oligoteratozoospermia; ROC: receiver operating characteristic; ROS: reactive oxygen species; TS: teratozoospermia; WHO: world health organization.
T2  - Systems Biology in Reproductive Medicine
T1  - Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis.
DO  - 10.1080/19396368.2019.1600171
ER  - 

@article{
author = "Otašević, Vesna and Kalezić, Anđelika and Macanović, Biljana and Janković, Aleksandra and Stančić, Ana and Garalejić, Eliana and Korać, Aleksandra and Korać, Bato",
year = "2019",
abstract = "Protein expression/activity of antioxidative defense enzymes (AD) in seminal plasma of fertile men might be used as biomarkers of male fertility status. To test this concept, the present study examined the semen parameters of males among 14 normal idiopathic (normozoospermia) and 84 subnormal (teratozoospermia, oligoteratozoospermia, oligoasthenoteratozoospermia) infertile individuals\. We investigated levels of protein expression/activity of Cu, Zn superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GSH-Px), their association with functional sperm parameters, as well as their potential to serve as biomarkers of specific sperm pathologies. Although the activity of CuZnSOD and protein expression of catalase were significantly correlated with several sperm parameters, underlying their potential role in etiology of various sperm abnormalities, investigation of their potential usefulness as a biomarker of semen quality showed that these AD enzymes could not distinguish subtle differences between various sperm pathologies. In contrast, GSH-Px activity was decreased in all groups with sperm pathologies and was a very good indicator of aberrations in functional sperm parameters, explaining up to 94.6% of infertility cases where functional sperm parameters were affected. Therefore, assessment of GSH-Px activity showed the potential to discriminate between infertile males with normal and subnormal semen characteristics and may prove useful in the evaluation of male (in)fertility. Abbreviations: AD: antioxidative defense; Cu, Zn SOD: copper, zinc superoxide dismutase; GSH-Px: glutathione peroxidase; MnSOD: manganese superoxide dismutase; NS: normospermia; OATS: oligoasthenoteratozoospermia; OTS: oligoteratozoospermia; ROC: receiver operating characteristic; ROS: reactive oxygen species; TS: teratozoospermia; WHO: world health organization.",
journal = "Systems Biology in Reproductive Medicine",
title = "Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis.",
doi = "10.1080/19396368.2019.1600171"
}

Otašević, V., Kalezić, A., Macanović, B., Janković, A., Stančić, A., Garalejić, E., Korać, A.,& Korać, B.. (2019). Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis.. in Systems Biology in Reproductive Medicine.
https://doi.org/10.1080/19396368.2019.1600171

Otašević V, Kalezić A, Macanović B, Janković A, Stančić A, Garalejić E, Korać A, Korać B. Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis.. in Systems Biology in Reproductive Medicine. 2019;.
doi:10.1080/19396368.2019.1600171 .

Otašević, Vesna, Kalezić, Anđelika, Macanović, Biljana, Janković, Aleksandra, Stančić, Ana, Garalejić, Eliana, Korać, Aleksandra, Korać, Bato, "Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis." in Systems Biology in Reproductive Medicine (2019),
https://doi.org/10.1080/19396368.2019.1600171 . .

TY  - JOUR
AU  - Kalezić, Anđelika
AU  - Macanović, Biljana
AU  - Garalejić, Eliana
AU  - Korać, Aleksandra
AU  - Otašević, Vesna
AU  - Korać, Bato
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0009279718304654?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3109
AB  - Nitric oxide (NO) is a signaling molecule responsible for initiation of molecular events that influence sperm functionality in a concentration-dependent manner. It is still not fully understood how seminal plasma NO contributes to sperm pathologies. The aim of this study was to elucidate whether and how NO is implicated in etiology of different sperm abnormalities. To this end we determine NO, nitrite and 3-nitrotyrosine (3-NT) content in seminal plasma of infertile men with specific pathologies (terato-, oligoterato- and oligoasthenoteratospermia) and relate it to infertile normospermic samples. To gain further understanding of NO metabolism in seminal plasma we determine protein expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). Here we show that NO, nitrite and 3-NT levels in seminal plasma of men with suboptimal semen parameters are significantly lower compared to normospermic men. An increase in protein expression of eNOS and no change in protein expression of iNOS is observed in men with sperm pathologies. Association of seminal plasma 3-NT level with functional sperm parameters is observed - positive correlation with sperm count, motility and morphology in normospermia, teratospermia and oligoteratospermia, as well as negative correlation with sperm morphology and motility in oligoasthenoteratospermia. Present study revealed that suboptimal seminal plasma NO content is found in all examined sperm pathologies. This result unequivocally shows the importance of NO for sperm function and involvement of suboptimal NO level in etiology of sperm abnormalities. Lower seminal plasma 3-NT level and its significant association with sperm parameters, found in pathologies, strongly indicates that protein nitration is important for spermatozoa function and that failure to establish this post-translational protein modification might be involved in etiology of sperm abnormalities. According to our results, NO measurement can discriminate infertile men with sperm pathologies from infertile normospermic men but is not indicative of a specific type of sperm pathology.
T2  - Chemico-Biological Interactions
T1  - Level of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphology.
VL  - 291
DO  - 10.1016/j.cbi.2018.07.002
SP  - 264
EP  - 270
ER  - 

@article{
author = "Kalezić, Anđelika and Macanović, Biljana and Garalejić, Eliana and Korać, Aleksandra and Otašević, Vesna and Korać, Bato",
year = "2018",
abstract = "Nitric oxide (NO) is a signaling molecule responsible for initiation of molecular events that influence sperm functionality in a concentration-dependent manner. It is still not fully understood how seminal plasma NO contributes to sperm pathologies. The aim of this study was to elucidate whether and how NO is implicated in etiology of different sperm abnormalities. To this end we determine NO, nitrite and 3-nitrotyrosine (3-NT) content in seminal plasma of infertile men with specific pathologies (terato-, oligoterato- and oligoasthenoteratospermia) and relate it to infertile normospermic samples. To gain further understanding of NO metabolism in seminal plasma we determine protein expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). Here we show that NO, nitrite and 3-NT levels in seminal plasma of men with suboptimal semen parameters are significantly lower compared to normospermic men. An increase in protein expression of eNOS and no change in protein expression of iNOS is observed in men with sperm pathologies. Association of seminal plasma 3-NT level with functional sperm parameters is observed - positive correlation with sperm count, motility and morphology in normospermia, teratospermia and oligoteratospermia, as well as negative correlation with sperm morphology and motility in oligoasthenoteratospermia. Present study revealed that suboptimal seminal plasma NO content is found in all examined sperm pathologies. This result unequivocally shows the importance of NO for sperm function and involvement of suboptimal NO level in etiology of sperm abnormalities. Lower seminal plasma 3-NT level and its significant association with sperm parameters, found in pathologies, strongly indicates that protein nitration is important for spermatozoa function and that failure to establish this post-translational protein modification might be involved in etiology of sperm abnormalities. According to our results, NO measurement can discriminate infertile men with sperm pathologies from infertile normospermic men but is not indicative of a specific type of sperm pathology.",
journal = "Chemico-Biological Interactions",
title = "Level of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphology.",
volume = "291",
doi = "10.1016/j.cbi.2018.07.002",
pages = "264-270"
}

Kalezić, A., Macanović, B., Garalejić, E., Korać, A., Otašević, V.,& Korać, B.. (2018). Level of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphology.. in Chemico-Biological Interactions, 291, 264-270.
https://doi.org/10.1016/j.cbi.2018.07.002

Kalezić A, Macanović B, Garalejić E, Korać A, Otašević V, Korać B. Level of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphology.. in Chemico-Biological Interactions. 2018;291:264-270.
doi:10.1016/j.cbi.2018.07.002 .

Kalezić, Anđelika, Macanović, Biljana, Garalejić, Eliana, Korać, Aleksandra, Otašević, Vesna, Korać, Bato, "Level of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphology." in Chemico-Biological Interactions, 291 (2018):264-270,
https://doi.org/10.1016/j.cbi.2018.07.002 . .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Macanović, Biljana
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1067
AB  - Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.
T2  - Antioxidants & Redox Signaling
T1  - Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?
IS  - 2
VL  - 18
SP  - 23
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1067
ER  - 

@article{
author = "Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Macanović, Biljana and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2013",
abstract = "Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.",
journal = "Antioxidants & Redox Signaling",
title = "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?",
number = "2",
volume = "18",
pages = "23-178",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1067"
}

Otašević, V., Korac, A. B., Vučetić, M., Macanović, B., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2013). Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling, 18(2), 23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067

Otašević V, Korac AB, Vučetić M, Macanović B, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling. 2013;18(2):23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Macanović, Biljana, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?" in Antioxidants & Redox Signaling, 18, no. 2 (2013):23-178,
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

TY  - CONF
AU  - Macanović, Biljana
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1212
C3  - Human Reproduction
T1  - Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO
IS  - null
VL  - 27
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1212
ER  - 

@conference{
author = "Macanović, Biljana and Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2012",
journal = "Human Reproduction",
title = "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO",
number = "null",
volume = "27",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1212"
}

Macanović, B., Otašević, V., Korac, A. B., Vučetić, M., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2012). Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction, 27(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1212

Macanović B, Otašević V, Korac AB, Vučetić M, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction. 2012;27(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

Macanović, Biljana, Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO" in Human Reproduction, 27, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .