TY  - JOUR
AU  - Stanojević, Maja
AU  - Ćirković, Valentina
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stamenković, Gorana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S187603411930108X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3316
AB  - BACKGROUND Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. METHODS Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral antibodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. RESULTS PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. CONCLUSIONS Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation.
T2  - Journal of Infection and Public Health
T1  - Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.
DO  - 10.1016/j.jiph.2019.02.021
ER  - 

@article{
author = "Stanojević, Maja and Ćirković, Valentina and Siljić, Marina and Gligić, Ana and Stamenković, Gorana",
year = "2019",
abstract = "BACKGROUND Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. METHODS Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral antibodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. RESULTS PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. CONCLUSIONS Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation.",
journal = "Journal of Infection and Public Health",
title = "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.",
doi = "10.1016/j.jiph.2019.02.021"
}

Stanojević, M., Ćirković, V., Siljić, M., Gligić, A.,& Stamenković, G.. (2019). Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.. in Journal of Infection and Public Health.
https://doi.org/10.1016/j.jiph.2019.02.021

Stanojević M, Ćirković V, Siljić M, Gligić A, Stamenković G. Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.. in Journal of Infection and Public Health. 2019;.
doi:10.1016/j.jiph.2019.02.021 .

Stanojević, Maja, Ćirković, Valentina, Siljić, Marina, Gligić, Ana, Stamenković, Gorana, "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011." in Journal of Infection and Public Health (2019),
https://doi.org/10.1016/j.jiph.2019.02.021 . .