TY  - CONF
AU  - Kirschner, Alexander K.T.
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Zarfel, Gernot
AU  - Koller, Michael
AU  - Kittinger, Clemens
AU  - Tóth, Erika
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Blaschke, Alfred Paul
AU  - Derx, Julia
AU  - Linke, Rita
AU  - Demeter, Katalin
AU  - Reischer, Georg
AU  - Savio, Domenico
AU  - Farnleitner, Andreas H.
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6232
AB  - Sustainable and target-oriented microbiological water quality management of rivers needs information on
whole river systems, especially if catchments are large and international. The microbiological water quality
of rivers is of uttermost relevance for human health as river water is used for several purposes (recreation,
drinking water production, irrigation). Besides information on faecal pollution levels, the origin of faecal
pollution and the assessment of associated infection- and health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and discusses future challenges and perspectives (pollution-scenario modelling, infection-risk assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS. Beside standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput amplicon-sequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big picture”
of the microbiological pollution status of large rivers. Harmonised trans-border microbiological water quality aps for the whole navigable Danube were established. MST marker analysis elucidated that the main faecal pollution source along the whole river is human wastewater. Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the potential health impacts of AMR associated with faecal pollution.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Faecal pollution and antibiotic resistance in the Danube River: Vision, Concepts and Lessons learned from Joint Danube Surveys
SP  - 144
EP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6232
ER  - 

@conference{
author = "Kirschner, Alexander K.T. and Schachner, Iris and Jakwerth, Stefan and Zarfel, Gernot and Koller, Michael and Kittinger, Clemens and Tóth, Erika and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Blaschke, Alfred Paul and Derx, Julia and Linke, Rita and Demeter, Katalin and Reischer, Georg and Savio, Domenico and Farnleitner, Andreas H.",
year = "2022",
abstract = "Sustainable and target-oriented microbiological water quality management of rivers needs information on
whole river systems, especially if catchments are large and international. The microbiological water quality
of rivers is of uttermost relevance for human health as river water is used for several purposes (recreation,
drinking water production, irrigation). Besides information on faecal pollution levels, the origin of faecal
pollution and the assessment of associated infection- and health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and discusses future challenges and perspectives (pollution-scenario modelling, infection-risk assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS. Beside standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput amplicon-sequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big picture”
of the microbiological pollution status of large rivers. Harmonised trans-border microbiological water quality aps for the whole navigable Danube were established. MST marker analysis elucidated that the main faecal pollution source along the whole river is human wastewater. Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the potential health impacts of AMR associated with faecal pollution.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Faecal pollution and antibiotic resistance in the Danube River: Vision, Concepts and Lessons learned from Joint Danube Surveys",
pages = "144-145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6232"
}

Kirschner, A. K.T., Schachner, I., Jakwerth, S., Zarfel, G., Koller, M., Kittinger, C., Tóth, E., Kolarević, S., Kračun-Kolarević, M., Blaschke, A. P., Derx, J., Linke, R., Demeter, K., Reischer, G., Savio, D.,& Farnleitner, A. H.. (2022). Faecal pollution and antibiotic resistance in the Danube River: Vision, Concepts and Lessons learned from Joint Danube Surveys. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 144-145.
https://hdl.handle.net/21.15107/rcub_ibiss_6232

Kirschner AK, Schachner I, Jakwerth S, Zarfel G, Koller M, Kittinger C, Tóth E, Kolarević S, Kračun-Kolarević M, Blaschke AP, Derx J, Linke R, Demeter K, Reischer G, Savio D, Farnleitner AH. Faecal pollution and antibiotic resistance in the Danube River: Vision, Concepts and Lessons learned from Joint Danube Surveys. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:144-145.
https://hdl.handle.net/21.15107/rcub_ibiss_6232 .

Kirschner, Alexander K.T., Schachner, Iris, Jakwerth, Stefan, Zarfel, Gernot, Koller, Michael, Kittinger, Clemens, Tóth, Erika, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Blaschke, Alfred Paul, Derx, Julia, Linke, Rita, Demeter, Katalin, Reischer, Georg, Savio, Domenico, Farnleitner, Andreas H., "Faecal pollution and antibiotic resistance in the Danube River: Vision, Concepts and Lessons learned from Joint Danube Surveys" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):144-145,
https://hdl.handle.net/21.15107/rcub_ibiss_6232 .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović, Jovana
AU  - Ilić, Marija
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, Sandra
AU  - Joksimović, Danijela
AU  - Pešić, Vladimir
AU  - Kirschner, Alexander K. T.
AU  - Linke, Rita
AU  - Ixenmaier, Simone
AU  - Farnleitner, Andreas
AU  - Savio, Domenico
AU  - Reischer, Georg
AU  - Tomić, Nevenka
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - https://link.springer.com/chapter/10.1007/698_2019_420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3952
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer
T2  - The Rivers of Montenegro
T2  - The Rivers of Montenegro
T1  - Microbiological Water Quality of Rivers in Montenegro
DO  - 10.1007/698_2019_420
SP  - 135
EP  - 155
ER  - 

@inbook{
editor = "Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G.",
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović, Jovana and Ilić, Marija and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, Sandra and Joksimović, Danijela and Pešić, Vladimir and Kirschner, Alexander K. T. and Linke, Rita and Ixenmaier, Simone and Farnleitner, Andreas and Savio, Domenico and Reischer, Georg and Tomić, Nevenka and Vuković-Gačić, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer",
journal = "The Rivers of Montenegro, The Rivers of Montenegro",
booktitle = "Microbiological Water Quality of Rivers in Montenegro",
doi = "10.1007/698_2019_420",
pages = "135-155"
}

Pešić, V., Paunović, M., Kostianoy, A. G., Kolarević, S., Kračun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A. K. T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vuković-Gačić, B.. (2020). Microbiological Water Quality of Rivers in Montenegro. in The Rivers of Montenegro
Springer., 135-155.
https://doi.org/10.1007/698_2019_420

Pešić V, Paunović M, Kostianoy AG, Kolarević S, Kračun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner AKT, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vuković-Gačić B. Microbiological Water Quality of Rivers in Montenegro. in The Rivers of Montenegro. 2020;:135-155.
doi:10.1007/698_2019_420 .

Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G., Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović, Jovana, Ilić, Marija, Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, Sandra, Joksimović, Danijela, Pešić, Vladimir, Kirschner, Alexander K. T., Linke, Rita, Ixenmaier, Simone, Farnleitner, Andreas, Savio, Domenico, Reischer, Georg, Tomić, Nevenka, Vuković-Gačić, Branka, "Microbiological Water Quality of Rivers in Montenegro" in The Rivers of Montenegro (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .

TY  - JOUR
AU  - Vrzel, Janja
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Ogrinc, Nives
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969716316667
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84994378206&origin=SingleRecordEmailAlert&txGid=6CE299281CDB840158BFAC52EC5A2E1C.wsnAw8kcdt7IPYLO0V48gA:71#
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2908
AB  - Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (δ15NNO3 and δ18ONO3) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.
T2  - Science of The Total Environment
T2  - Science of The Total Environment
T1  - Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.213
SP  - 1460
EP  - 1471
ER  - 

@article{
author = "Vrzel, Janja and Vuković-Gačić, Branka and Kolarević, Stoimir and Gačić, Zoran and Kračun-Kolarević, Margareta and Kostić, Jovana and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Ogrinc, Nives",
year = "2016",
abstract = "Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (δ15NNO3 and δ18ONO3) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.",
journal = "Science of The Total Environment, Science of The Total Environment",
title = "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.213",
pages = "1460-1471"
}

Vrzel, J., Vuković-Gačić, B., Kolarević, S., Gačić, Z., Kračun-Kolarević, M., Kostić, J., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Ogrinc, N.. (2016). Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of The Total Environment, 573, 1460-1471.
https://doi.org/10.1016/j.scitotenv.2016.07.213

Vrzel J, Vuković-Gačić B, Kolarević S, Gačić Z, Kračun-Kolarević M, Kostić J, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Ogrinc N. Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of The Total Environment. 2016;573:1460-1471.
doi:10.1016/j.scitotenv.2016.07.213 .

Vrzel, Janja, Vuković-Gačić, Branka, Kolarević, Stoimir, Gačić, Zoran, Kračun-Kolarević, Margareta, Kostić, Jovana, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Ogrinc, Nives, "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin" in Science of The Total Environment, 573 (2016):1460-1471,
https://doi.org/10.1016/j.scitotenv.2016.07.213 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Simonović, Predrag
AU  - Simić, Vladica
AU  - Milošković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milačič, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://dx.plos.org/10.1371/journal.pone.0162450
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84992314127&origin=SingleRecordEmailAlert&txGid=06CA159BD204EA9525A7DB2D67E8F57F.wsnAw8kcdt7IPYLO0V48gA:19
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2503
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimuriumTA1535/ pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/ Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrialwastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
T2  - Plos ONE
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
SP  - e0162450
EP  - e0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kračun-Kolarević, Margareta and Kostić, Jovana and Simonović, Predrag and Simić, Vladica and Milošković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milačič, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimuriumTA1535/ pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/ Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrialwastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
journal = "Plos ONE",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450",
pages = "e0162450-e0162450"
}

Kolarević, S., Aborgiba, M., Kračun-Kolarević, M., Kostić, J., Simonović, P., Simić, V., Milošković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milačič, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vuković-Gačić, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in Plos ONE, 11(9), e0162450-e0162450.
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kračun-Kolarević M, Kostić J, Simonović P, Simić V, Milošković A, Reischer G, Farnleitner A, Gačić Z, Milačič R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vuković-Gačić B. Evaluation of Genotoxic Pressure along the Sava River. in Plos ONE. 2016;11(9):e0162450-e0162450.
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kračun-Kolarević, Margareta, Kostić, Jovana, Simonović, Predrag, Simić, Vladica, Milošković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milačič, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in Plos ONE, 11, no. 9 (2016):e0162450-e0162450,
https://doi.org/10.1371/journal.pone.0162450 . .