TY  - CONF
AU  - Koller, Michael
AU  - Nürnberger, Julia
AU  - Hack, Kerstin
AU  - Dielacher, Irina
AU  - Wachter, Julian
AU  - Hoffer, Benjamin
AU  - Kohlmaier, Sophie
AU  - Hagendorfer, Christin
AU  - Kapic, Medina
AU  - Julia, Gütl
AU  - Arsenijevic, Polina
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Tóth, Erika
AU  - Savio, Domenico
AU  - Farnleitner, Andreas H.
AU  - Kittinger, Clemens
AU  - Kirschner, Alexander K.T.
AU  - Zarfel, Gernot
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6231
AB  - Human induced antibiotic resistant bacteria (ARB) are not only found in clinical surroundings: large rivers are of great concern as regards their spreading. This ongoing study´s aim is to analyse the major propagation pathways and sources of ARB in the Danube, and to compare the results with data obtained in 2013. Escherichia coli and Klebsiella spp. isolated during the 4th Joint Danube Survey were tested for their antibiotic susceptibility. 23.2% of 1635 E. coli were resistant and 11.7% were multiresistant. There was a significant increase in resistances to augmentin, moxifloxacin and piperacillin/tazobactam and a significant decrease regarding tetracycline. 23 expressed an extended-spectrum-beta-lactamase phenotype. 15.87 % of 630 Klebsiella spp. were resistant and 0.95% were multiresistant. Resistances
to moxifloxacin and augmentin were the most common resistances detected.
Two isolates showed an ESBL-phenotype, and two isolates were resistant to the last line antibiotic tigecycline. In comparison the data of 2013 and 2019 show a similar proportion for multiresistant E. coli, but regarding resistances to some single antibiotics significant changes were determined. In contrast, the Klebsiella isolates showed a very similar distribution of resistances. Despite, 30% of non-wild type resistant E. coli in the environment is a very high number when compared to clinical settings with approximately 50%. However, the relevance of these findings concerning human health and the environment is yet unclear as there is only a very limited number of studies for comparison and studies on the significance of pathways from the river back to humans are largely missing.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Monitoring non-wild type antibiotic resistant Enterobacteriaceae in the River Danube: a six-year-comparison
SP  - 64
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6231
ER  - 

@conference{
author = "Koller, Michael and Nürnberger, Julia and Hack, Kerstin and Dielacher, Irina and Wachter, Julian and Hoffer, Benjamin and Kohlmaier, Sophie and Hagendorfer, Christin and Kapic, Medina and Julia, Gütl and Arsenijevic, Polina and Schachner, Iris and Jakwerth, Stefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Tóth, Erika and Savio, Domenico and Farnleitner, Andreas H. and Kittinger, Clemens and Kirschner, Alexander K.T. and Zarfel, Gernot",
year = "2022",
abstract = "Human induced antibiotic resistant bacteria (ARB) are not only found in clinical surroundings: large rivers are of great concern as regards their spreading. This ongoing study´s aim is to analyse the major propagation pathways and sources of ARB in the Danube, and to compare the results with data obtained in 2013. Escherichia coli and Klebsiella spp. isolated during the 4th Joint Danube Survey were tested for their antibiotic susceptibility. 23.2% of 1635 E. coli were resistant and 11.7% were multiresistant. There was a significant increase in resistances to augmentin, moxifloxacin and piperacillin/tazobactam and a significant decrease regarding tetracycline. 23 expressed an extended-spectrum-beta-lactamase phenotype. 15.87 % of 630 Klebsiella spp. were resistant and 0.95% were multiresistant. Resistances
to moxifloxacin and augmentin were the most common resistances detected.
Two isolates showed an ESBL-phenotype, and two isolates were resistant to the last line antibiotic tigecycline. In comparison the data of 2013 and 2019 show a similar proportion for multiresistant E. coli, but regarding resistances to some single antibiotics significant changes were determined. In contrast, the Klebsiella isolates showed a very similar distribution of resistances. Despite, 30% of non-wild type resistant E. coli in the environment is a very high number when compared to clinical settings with approximately 50%. However, the relevance of these findings concerning human health and the environment is yet unclear as there is only a very limited number of studies for comparison and studies on the significance of pathways from the river back to humans are largely missing.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Monitoring non-wild type antibiotic resistant Enterobacteriaceae in the River Danube: a six-year-comparison",
pages = "64-65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6231"
}

Koller, M., Nürnberger, J., Hack, K., Dielacher, I., Wachter, J., Hoffer, B., Kohlmaier, S., Hagendorfer, C., Kapic, M., Julia, G., Arsenijevic, P., Schachner, I., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Tóth, E., Savio, D., Farnleitner, A. H., Kittinger, C., Kirschner, A. K.T.,& Zarfel, G.. (2022). Monitoring non-wild type antibiotic resistant Enterobacteriaceae in the River Danube: a six-year-comparison. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 64-65.
https://hdl.handle.net/21.15107/rcub_ibiss_6231

Koller M, Nürnberger J, Hack K, Dielacher I, Wachter J, Hoffer B, Kohlmaier S, Hagendorfer C, Kapic M, Julia G, Arsenijevic P, Schachner I, Jakwerth S, Kolarević S, Kračun-Kolarević M, Tóth E, Savio D, Farnleitner AH, Kittinger C, Kirschner AK, Zarfel G. Monitoring non-wild type antibiotic resistant Enterobacteriaceae in the River Danube: a six-year-comparison. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:64-65.
https://hdl.handle.net/21.15107/rcub_ibiss_6231 .

Koller, Michael, Nürnberger, Julia, Hack, Kerstin, Dielacher, Irina, Wachter, Julian, Hoffer, Benjamin, Kohlmaier, Sophie, Hagendorfer, Christin, Kapic, Medina, Julia, Gütl, Arsenijevic, Polina, Schachner, Iris, Jakwerth, Stefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Tóth, Erika, Savio, Domenico, Farnleitner, Andreas H., Kittinger, Clemens, Kirschner, Alexander K.T., Zarfel, Gernot, "Monitoring non-wild type antibiotic resistant Enterobacteriaceae in the River Danube: a six-year-comparison" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):64-65,
https://hdl.handle.net/21.15107/rcub_ibiss_6231 .

TY  - CONF
AU  - Koller, Michael
AU  - Hack, Kerstin
AU  - Dielacher, Irina
AU  - Wachter, Julian
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Tóth, Erika
AU  - Savio, Domenico
AU  - Farnleitner, Andreas H.
AU  - Kittinger, Clemens
AU  - Kirschner, Alexander K.T.
AU  - Zarfel, Gernot
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4730
AB  - Introduction
The emerging occurrence of human induced antibiotic resistant bacteria (ARB) is not only limited to
clinical surroundings: they can also be found in the human population, animals and the water
environment. Large rivers are of great concern as regards the spreading of ARB. Thus, this ongoing
study ́s aim is to analyze the major propagation pathways and sources of ARB in the Danube, and to
do a six-year-comparison with data obtained in 2013.
Study design and methods
Within the frame of the 4th Joint Danube Survey (JDS) of the International Commission for the
Protection of the Danube River (ICPDR), water samples were taken at 36 sampling points along the
whole Danube. Escherichia coli as clinically relevant organisms were isolated and tested for their
susceptibility to 20 different antibiotics.
Results
1298 E. coli isolates have been tested so far. 11.33 % of them were multiresistant and 23.19 % were
resistant to one or two classes of antibiotics. The preliminary data show a very similar pattern
compared to the data obtained in 2013 (629 isolates) when 9.70 % of the isolates were multiresistant
and 29.09 % were resistant to one or two classes of antibiotics. Most of the resistances are still to
ampicillin and tetracycline. There are no resistances to carbapenems, colistin, amikacin and tigecycline.
But there is a significant increase in resistances to amoxicillin with clavulanic acid, moxifloxacin and
piperacillin with tazobactam and a significant decrease regarding tetracycline. 21 isolates are
confirmed ESBL-producers while in 2013 there were four.
Discussion
In comparison the data of 2013 and 2019 show a similar pattern regarding multiresistance. Regarding
resistances to single antibiotics significant changes could be determined. However, there are still many
of the isolates to be tested until the final analysis.
PB  - Neuburg:  Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4730
ER  - 

@conference{
author = "Koller, Michael and Hack, Kerstin and Dielacher, Irina and Wachter, Julian and Schachner, Iris and Jakwerth, Stefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Tóth, Erika and Savio, Domenico and Farnleitner, Andreas H. and Kittinger, Clemens and Kirschner, Alexander K.T. and Zarfel, Gernot",
year = "2021",
abstract = "Introduction
The emerging occurrence of human induced antibiotic resistant bacteria (ARB) is not only limited to
clinical surroundings: they can also be found in the human population, animals and the water
environment. Large rivers are of great concern as regards the spreading of ARB. Thus, this ongoing
study ́s aim is to analyze the major propagation pathways and sources of ARB in the Danube, and to
do a six-year-comparison with data obtained in 2013.
Study design and methods
Within the frame of the 4th Joint Danube Survey (JDS) of the International Commission for the
Protection of the Danube River (ICPDR), water samples were taken at 36 sampling points along the
whole Danube. Escherichia coli as clinically relevant organisms were isolated and tested for their
susceptibility to 20 different antibiotics.
Results
1298 E. coli isolates have been tested so far. 11.33 % of them were multiresistant and 23.19 % were
resistant to one or two classes of antibiotics. The preliminary data show a very similar pattern
compared to the data obtained in 2013 (629 isolates) when 9.70 % of the isolates were multiresistant
and 29.09 % were resistant to one or two classes of antibiotics. Most of the resistances are still to
ampicillin and tetracycline. There are no resistances to carbapenems, colistin, amikacin and tigecycline.
But there is a significant increase in resistances to amoxicillin with clavulanic acid, moxifloxacin and
piperacillin with tazobactam and a significant decrease regarding tetracycline. 21 isolates are
confirmed ESBL-producers while in 2013 there were four.
Discussion
In comparison the data of 2013 and 2019 show a similar pattern regarding multiresistance. Regarding
resistances to single antibiotics significant changes could be determined. However, there are still many
of the isolates to be tested until the final analysis.",
publisher = "Neuburg:  Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4730"
}

Koller, M., Hack, K., Dielacher, I., Wachter, J., Schachner, I., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Tóth, E., Savio, D., Farnleitner, A. H., Kittinger, C., Kirschner, A. K.T.,& Zarfel, G.. (2021). Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Neuburg:  Aueninstitut Neuburg., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_4730

Koller M, Hack K, Dielacher I, Wachter J, Schachner I, Jakwerth S, Kolarević S, Kračun-Kolarević M, Tóth E, Savio D, Farnleitner AH, Kittinger C, Kirschner AK, Zarfel G. Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_4730 .

Koller, Michael, Hack, Kerstin, Dielacher, Irina, Wachter, Julian, Schachner, Iris, Jakwerth, Stefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Tóth, Erika, Savio, Domenico, Farnleitner, Andreas H., Kittinger, Clemens, Kirschner, Alexander K.T., Zarfel, Gernot, "Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison" in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):30,
https://hdl.handle.net/21.15107/rcub_ibiss_4730 .