TY  - JOUR
AU  - Cvetanović Kljakić, Aleksandra
AU  - Stupar, Alena
AU  - Terzić, Milena
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Yildiztugay, Evren
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6468
AB  - The plant species Opopanax hispidus is known in traditional Turkish medicine and nutrition. In addition to all the advantages that are known by the traditions of old generations, O. hispidus has been insufficiently examined, and its potential could be used in treatment in modern medicine and diet. In this paper, O. hispidus extracts obtained by conventional (maceration (MAC), and Soxhlet (SOX)) and contemporary (accelerated solvent extraction (ASE), homogenizer assisted extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SFE)) methods were examined in terms of their chemical composition and biological potential. The extract obtained by HAE extraction had the highest content of total phenolics and flavonoids (36.98 mg GAE/g and 15.04 mg RE/g, respectively). The highest antioxidant activity was recorded for HAE and ASE extracts. O. hispidus extracts achieved inhibitory activity against acetylcholinesterase (4.12–6.44 mg GALAE/g), tyrosinase (44.78–76.72 mg KAE/g), α-amylase (0.35–0.46 mmol ACAE/g), and α-glucosidase (1.67–1.77 mmol ACAE/g) enzymes. LC-MS technique was used for the quantitative analysis of the extracts, allowing the quantification of chlorogenic acid, neochlorogenic acid, and p-coumaric acid, rutin, luteolin, and kaempferol 3-O-glucoside as the most abundant phenolic components. Results suggested that the green extraction techniques for obtaining extracts that are rich in bioactive compounds have advantages compared to conventional extraction. Therefore, these extracts can be used as antioxidants, neuroprotective, antityrosinase, and antidiabetic agents while respecting the principles of sustainable development and green chemistry.
PB  - Elsevier
T2  - Sustainable Chemistry and Pharmacy
T1  - Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies
VL  - 33
DO  - 10.1016/j.scp.2023.101122
SP  - 101122
ER  - 

@article{
author = "Cvetanović Kljakić, Aleksandra and Stupar, Alena and Terzić, Milena and Božunović, Jelena and Gašić, Uroš and Zengin, Gokhan and Yildiztugay, Evren",
year = "2023",
abstract = "The plant species Opopanax hispidus is known in traditional Turkish medicine and nutrition. In addition to all the advantages that are known by the traditions of old generations, O. hispidus has been insufficiently examined, and its potential could be used in treatment in modern medicine and diet. In this paper, O. hispidus extracts obtained by conventional (maceration (MAC), and Soxhlet (SOX)) and contemporary (accelerated solvent extraction (ASE), homogenizer assisted extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SFE)) methods were examined in terms of their chemical composition and biological potential. The extract obtained by HAE extraction had the highest content of total phenolics and flavonoids (36.98 mg GAE/g and 15.04 mg RE/g, respectively). The highest antioxidant activity was recorded for HAE and ASE extracts. O. hispidus extracts achieved inhibitory activity against acetylcholinesterase (4.12–6.44 mg GALAE/g), tyrosinase (44.78–76.72 mg KAE/g), α-amylase (0.35–0.46 mmol ACAE/g), and α-glucosidase (1.67–1.77 mmol ACAE/g) enzymes. LC-MS technique was used for the quantitative analysis of the extracts, allowing the quantification of chlorogenic acid, neochlorogenic acid, and p-coumaric acid, rutin, luteolin, and kaempferol 3-O-glucoside as the most abundant phenolic components. Results suggested that the green extraction techniques for obtaining extracts that are rich in bioactive compounds have advantages compared to conventional extraction. Therefore, these extracts can be used as antioxidants, neuroprotective, antityrosinase, and antidiabetic agents while respecting the principles of sustainable development and green chemistry.",
publisher = "Elsevier",
journal = "Sustainable Chemistry and Pharmacy",
title = "Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies",
volume = "33",
doi = "10.1016/j.scp.2023.101122",
pages = "101122"
}

Cvetanović Kljakić, A., Stupar, A., Terzić, M., Božunović, J., Gašić, U., Zengin, G.,& Yildiztugay, E.. (2023). Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies. in Sustainable Chemistry and Pharmacy
Elsevier., 33, 101122.
https://doi.org/10.1016/j.scp.2023.101122

Cvetanović Kljakić A, Stupar A, Terzić M, Božunović J, Gašić U, Zengin G, Yildiztugay E. Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies. in Sustainable Chemistry and Pharmacy. 2023;33:101122.
doi:10.1016/j.scp.2023.101122 .

Cvetanović Kljakić, Aleksandra, Stupar, Alena, Terzić, Milena, Božunović, Jelena, Gašić, Uroš, Zengin, Gokhan, Yildiztugay, Evren, "Chemical profiling and biological activities of Opopanax hispidus extracts: A comparative insight on conventional and green extraction technologies" in Sustainable Chemistry and Pharmacy, 33 (2023):101122,
https://doi.org/10.1016/j.scp.2023.101122 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Stupar, Alena
AU  - Uysal, Ahmet
AU  - Şenkardes, Ismail
AU  - Sinan, Kouadio Ibrahime
AU  - Picot-Allain, Marie Carene Nancy
AU  - Ak, Gunes
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3769
AB  - The growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction
methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach.
The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic,
antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using
conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted
(UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical char-
acterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass
spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested
extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE
(170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid
content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS
(679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme in-
hibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent
[GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent
[KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial
properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive com-
pounds from Origanum vulgare subsp. viridulum aerial parts.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods
VL  - 154
DO  - 10.1016/j.indcrop.2020.112747
SP  - 112747
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Dragićević, Milan and Stupar, Alena and Uysal, Ahmet and Şenkardes, Ismail and Sinan, Kouadio Ibrahime and Picot-Allain, Marie Carene Nancy and Ak, Gunes and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "The growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction
methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach.
The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic,
antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using
conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted
(UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical char-
acterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass
spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested
extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE
(170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid
content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS
(679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme in-
hibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent
[GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent
[KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial
properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive com-
pounds from Origanum vulgare subsp. viridulum aerial parts.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods",
volume = "154",
doi = "10.1016/j.indcrop.2020.112747",
pages = "112747"
}

Zengin, G., Cvetanović, A., Gašić, U., Dragićević, M., Stupar, A., Uysal, A., Şenkardes, I., Sinan, K. I., Picot-Allain, M. C. N., Ak, G.,& Mahomoodally, M. F.. (2020). UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods. in Industrial Crops & Products
Elsevier., 154, 112747.
https://doi.org/10.1016/j.indcrop.2020.112747

Zengin G, Cvetanović A, Gašić U, Dragićević M, Stupar A, Uysal A, Şenkardes I, Sinan KI, Picot-Allain MCN, Ak G, Mahomoodally MF. UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods. in Industrial Crops & Products. 2020;154:112747.
doi:10.1016/j.indcrop.2020.112747 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Dragićević, Milan, Stupar, Alena, Uysal, Ahmet, Şenkardes, Ismail, Sinan, Kouadio Ibrahime, Picot-Allain, Marie Carene Nancy, Ak, Gunes, Mahomoodally, Mohamad Fawzi, "UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods" in Industrial Crops & Products, 154 (2020):112747,
https://doi.org/10.1016/j.indcrop.2020.112747 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Cvetanović, Aleksandra
AU  - Gašić, Uroš
AU  - Stupar, Alena
AU  - Bulut, Gizem
AU  - Senkardes, Ismail
AU  - Dogan, Ahmet
AU  - Sinan, Kouadio Ibrahime
AU  - Uysal, Sengul
AU  - Aumeeruddy-Elalfi, Zaahiri
AU  - Aktumsek, Abdurrahman
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2020
UR  - https://doi.org/10.1016/j.indcrop.2020.112202
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3609
AB  - Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.
T2  - Industrial Crops & Products
T1  - Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip
IS  - April 2020
VL  - 146
DO  - 10.1016/j.indcrop.2020.112202
SP  - 112202
ER  - 

@article{
author = "Zengin, Gokhan and Cvetanović, Aleksandra and Gašić, Uroš and Stupar, Alena and Bulut, Gizem and Senkardes, Ismail and Dogan, Ahmet and Sinan, Kouadio Ibrahime and Uysal, Sengul and Aumeeruddy-Elalfi, Zaahiri and Aktumsek, Abdurrahman and Mahomoodally, Mohamad Fawzi",
year = "2020",
abstract = "Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and bio- logical propensities of extracts prepared from this plant. This study endeavors to highlight the biological po- tential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was es- tablished via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non- communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium ex- tracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.",
journal = "Industrial Crops & Products",
title = "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip",
number = "April 2020",
volume = "146",
doi = "10.1016/j.indcrop.2020.112202",
pages = "112202"
}

Zengin, G., Cvetanović, A., Gašić, U., Stupar, A., Bulut, G., Senkardes, I., Dogan, A., Sinan, K. I., Uysal, S., Aumeeruddy-Elalfi, Z., Aktumsek, A.,& Mahomoodally, M. F.. (2020). Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products, 146(April 2020), 112202.
https://doi.org/10.1016/j.indcrop.2020.112202

Zengin G, Cvetanović A, Gašić U, Stupar A, Bulut G, Senkardes I, Dogan A, Sinan KI, Uysal S, Aumeeruddy-Elalfi Z, Aktumsek A, Mahomoodally MF. Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip. in Industrial Crops & Products. 2020;146(April 2020):112202.
doi:10.1016/j.indcrop.2020.112202 .

Zengin, Gokhan, Cvetanović, Aleksandra, Gašić, Uroš, Stupar, Alena, Bulut, Gizem, Senkardes, Ismail, Dogan, Ahmet, Sinan, Kouadio Ibrahime, Uysal, Sengul, Aumeeruddy-Elalfi, Zaahiri, Aktumsek, Abdurrahman, Mahomoodally, Mohamad Fawzi, "Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip" in Industrial Crops & Products, 146, no. April 2020 (2020):112202,
https://doi.org/10.1016/j.indcrop.2020.112202 . .