TY  - JOUR
AU  - Despotović, Ana
AU  - Antonijević, Đorđe M
AU  - Ilić, Dragan
AU  - Zogović, Nevena
AU  - Jokanović, Vukoman R
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4430
AB  - Introduction Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials’ extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements’ extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions Novel formulation of CS cement – ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility.
AB  - Uvod Kalcijum-silikatni (KS) dentalni cementi se koriste u brojnim kliničkim indikacijama u stomatologiji koje uključuju direktno prekrivanje pulpe, retrogradnu hirurgiju korena zuba, lečenje perforacija i apeksogenezu/apeksifikaciju. Materijali i metode U istraživanju je korišćen novosintetisani cement na bazi KS sa dodatkom SrCO3 kao kontrastnog agensa ALBO-DENTA, dok su kao kontrola korišćeni cement Portland (PC, Aalborg, Denmark) i ProRoot MTA (MTA, Tulsa Dental, USA). Rendgenokontrasnost je ispitivana digitalnom radiografijom primenom aparata Trophy, sa namerom da se precizno odredi minimum
kontrastnog agensa koji zadovoljava zahteve standarda ISO za rendgenkontrastnost. Biokompatibilnost materijala je ispitana in vitro, u kulturi ćelija mišjeg fibrosarkoma L929 tretiranoj ekstraktima ispitivanih materijala. Ćelijska morfologija je praćena upotrebom fazno-kontrastne mikroskopije, dok je vijabilnost ćelija utvrđivana kristal violet (KV) i 3-(4,5-dimetiltiazol-2-yl)-2,5-difenfl-tetrazolium bromid (MTT) esejima.
Rezultati Ispitivanje rendgenkontrastnosti je pokazalo da dodatak 30% SrCO3 dovodi do zadovoljavajućeg kontrasta materijala (3,45 mm Al). Analiza citotoksičnosti KV i MTT metodom je pokazala da čisti ekstrakt ALBO-DENTA pokazuje bolju biokompatibilnost u poređenju sa PC i MTA, dok serijska razblaženja ekstrakta ispitivanog cementa, kao i PC i MTA, nisu uticala na vijabilitet ćelija L929. Zaključci Novi cement na bazi KS – ALBO-DENT pokazao je zavodovoljavajuću rendgenkontrastnost i odgovarajuću biokompatibilnost.
PB  - Belgrade: Serbian Dental Journal
T2  - Serbian Dental Journal
T1  - Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement
T1  - Ispitivanje rendgenkontrastnosti i citotoksičnosti ALBO-DENTA – novog kalcijum-silikatnog cementa sa dodatkom stroncijumkarbonata
IS  - 2
VL  - 68
DO  - 10.2298/SGS2102068D
SP  - 68
EP  - 78
ER  - 

@article{
author = "Despotović, Ana and Antonijević, Đorđe M and Ilić, Dragan and Zogović, Nevena and Jokanović, Vukoman R",
year = "2021",
abstract = "Introduction Calcium silicate (CS) dental cements have numerous clinical indications in dentistry including pulp capping, root end surgery, perforation repair and apexification/apexogenesis treatment. Materials and methods Novel CS based dental cement with incorporation of SrCO3 radiopacifier named ALBO-DENT was used as an experimental cement material while Portland cement (Aalborg, Denmark) and ProRoot MTA (Tulsa Dental, USA) were used as controls. The radiopacity evaluation was performed using digital Trophy Radiographic system with an intention to precisely determine the minimum of radiopaque agent needed to confer to ISO radiopacity requirement. Thereafter, biocompatibility of material was tested in in vitro conditions in mouse fibrosarcoma L929 cell culture treated with materials’ extracts. Cell morphology was observed using phase-contrast microscopy, while cell viability was measured using crystal violet (CV) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. Results Radiopacity evaluation revealed that 30%wt addition of SrCO3 was necessary to achieve satisfactory radiopacity (3.45 mm Al). Cytotoxicity analysis using CV and MTT assays revealed that pure extracts of ALBO-DENT presented superior biocompatibility when compared to PC and MTA controls while serial dilutions of experimental cements’ extracts as well as that of PC and MTA did not influence L929 cell viability. Conclusions Novel formulation of CS cement – ALBO-DENT presented satisfactory radiopacity and adequate biocompatibility., Uvod Kalcijum-silikatni (KS) dentalni cementi se koriste u brojnim kliničkim indikacijama u stomatologiji koje uključuju direktno prekrivanje pulpe, retrogradnu hirurgiju korena zuba, lečenje perforacija i apeksogenezu/apeksifikaciju. Materijali i metode U istraživanju je korišćen novosintetisani cement na bazi KS sa dodatkom SrCO3 kao kontrastnog agensa ALBO-DENTA, dok su kao kontrola korišćeni cement Portland (PC, Aalborg, Denmark) i ProRoot MTA (MTA, Tulsa Dental, USA). Rendgenokontrasnost je ispitivana digitalnom radiografijom primenom aparata Trophy, sa namerom da se precizno odredi minimum
kontrastnog agensa koji zadovoljava zahteve standarda ISO za rendgenkontrastnost. Biokompatibilnost materijala je ispitana in vitro, u kulturi ćelija mišjeg fibrosarkoma L929 tretiranoj ekstraktima ispitivanih materijala. Ćelijska morfologija je praćena upotrebom fazno-kontrastne mikroskopije, dok je vijabilnost ćelija utvrđivana kristal violet (KV) i 3-(4,5-dimetiltiazol-2-yl)-2,5-difenfl-tetrazolium bromid (MTT) esejima.
Rezultati Ispitivanje rendgenkontrastnosti je pokazalo da dodatak 30% SrCO3 dovodi do zadovoljavajućeg kontrasta materijala (3,45 mm Al). Analiza citotoksičnosti KV i MTT metodom je pokazala da čisti ekstrakt ALBO-DENTA pokazuje bolju biokompatibilnost u poređenju sa PC i MTA, dok serijska razblaženja ekstrakta ispitivanog cementa, kao i PC i MTA, nisu uticala na vijabilitet ćelija L929. Zaključci Novi cement na bazi KS – ALBO-DENT pokazao je zavodovoljavajuću rendgenkontrastnost i odgovarajuću biokompatibilnost.",
publisher = "Belgrade: Serbian Dental Journal",
journal = "Serbian Dental Journal",
title = "Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement, Ispitivanje rendgenkontrastnosti i citotoksičnosti ALBO-DENTA – novog kalcijum-silikatnog cementa sa dodatkom stroncijumkarbonata",
number = "2",
volume = "68",
doi = "10.2298/SGS2102068D",
pages = "68-78"
}

Despotović, A., Antonijević, Đ. M., Ilić, D., Zogović, N.,& Jokanović, V. R.. (2021). Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement. in Serbian Dental Journal
Belgrade: Serbian Dental Journal., 68(2), 68-78.
https://doi.org/10.2298/SGS2102068D

Despotović A, Antonijević ĐM, Ilić D, Zogović N, Jokanović VR. Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement. in Serbian Dental Journal. 2021;68(2):68-78.
doi:10.2298/SGS2102068D .

Despotović, Ana, Antonijević, Đorđe M, Ilić, Dragan, Zogović, Nevena, Jokanović, Vukoman R, "Investigation of the radiopacity and cytotoxicity of ALBODENT – novel strontium carbonate incorporated calcium silicate based dental cement" in Serbian Dental Journal, 68, no. 2 (2021):68-78,
https://doi.org/10.2298/SGS2102068D . .

TY  - JOUR
AU  - Misirkić Marjanović, Maja
AU  - Todorović-Marković, Biljana M
AU  - Vučićević, Ljubica
AU  - Janjetović, Kristina
AU  - Jokanović, Vukoman R
AU  - Dramicanin, Miroslav D
AU  - Marković, Zoran M
AU  - Trajković, Vladimir S
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1456
AB  - The influence of fullerene (C-60) nanoparticles on the cytotoxicity of a highly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticles were prepared by mechanochemically assisted complexation with anionic surfactant sodium dodecyl sulfate, macrocyclic oligosaccharide gamma-cyclodextrin or the copolymer ethylene vinyl acetate-ethylene vinyl versatate. C-60 nanoparticles were characterized by UV-vis and atomic force microscopy. While readily internalized by mouse L929 fibroblasts, C-60 nanoparticles were not cytotoxic. Moreover, they partially protected L929 cells from the cytotoxic effect of NO-releasing compounds sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO) and 3-morpholino-sydnonimine (SIN-1). C-60 nanoparticles reduced SNP-induced apoptotic cell death by preventing mitochondrial depolarization, caspase activation, cell membrane phosphatidylserine exposure and DNA fragmentation. The protective action of C-60 nanoparticles was not exerted via direct interaction with NO, but through neutralization of mitochondria-produced superoxide radical in NO-treated cells, as demonstrated by using different redox-sensitive reporter fluorochromes. These data suggest that C-60 complexes with appropriate host molecules might be plausible candidates for preventing NO-mediated cell injury in inflammatory/autoimmune disorders. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles
IS  - 12
VL  - 30
EP  - 2328
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1456
ER  - 

@article{
author = "Misirkić Marjanović, Maja and Todorović-Marković, Biljana M and Vučićević, Ljubica and Janjetović, Kristina and Jokanović, Vukoman R and Dramicanin, Miroslav D and Marković, Zoran M and Trajković, Vladimir S",
year = "2009",
abstract = "The influence of fullerene (C-60) nanoparticles on the cytotoxicity of a highly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticles were prepared by mechanochemically assisted complexation with anionic surfactant sodium dodecyl sulfate, macrocyclic oligosaccharide gamma-cyclodextrin or the copolymer ethylene vinyl acetate-ethylene vinyl versatate. C-60 nanoparticles were characterized by UV-vis and atomic force microscopy. While readily internalized by mouse L929 fibroblasts, C-60 nanoparticles were not cytotoxic. Moreover, they partially protected L929 cells from the cytotoxic effect of NO-releasing compounds sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO) and 3-morpholino-sydnonimine (SIN-1). C-60 nanoparticles reduced SNP-induced apoptotic cell death by preventing mitochondrial depolarization, caspase activation, cell membrane phosphatidylserine exposure and DNA fragmentation. The protective action of C-60 nanoparticles was not exerted via direct interaction with NO, but through neutralization of mitochondria-produced superoxide radical in NO-treated cells, as demonstrated by using different redox-sensitive reporter fluorochromes. These data suggest that C-60 complexes with appropriate host molecules might be plausible candidates for preventing NO-mediated cell injury in inflammatory/autoimmune disorders. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles",
number = "12",
volume = "30",
pages = "2328",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1456"
}

Misirkić Marjanović, M., Todorović-Marković, B. M., Vučićević, L., Janjetović, K., Jokanović, V. R., Dramicanin, M. D., Marković, Z. M.,& Trajković, V. S.. (2009). The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles. in Biomaterials, 30(12).
https://hdl.handle.net/21.15107/rcub_ibiss_1456

Misirkić Marjanović M, Todorović-Marković BM, Vučićević L, Janjetović K, Jokanović VR, Dramicanin MD, Marković ZM, Trajković VS. The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles. in Biomaterials. 2009;30(12):null-2328.
https://hdl.handle.net/21.15107/rcub_ibiss_1456 .

Misirkić Marjanović, Maja, Todorović-Marković, Biljana M, Vučićević, Ljubica, Janjetović, Kristina, Jokanović, Vukoman R, Dramicanin, Miroslav D, Marković, Zoran M, Trajković, Vladimir S, "The protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticles" in Biomaterials, 30, no. 12 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1456 .