@conference{
author = "Mocan, Andrej and Pop, Anca and Fizesan, Ionel and Diuzheva, Alina and Carradori, Simone and Locatelli, Marcello and Soković, Marina and Zengin, Gokhan and Crisan, Gianina",
year = "2019",
abstract = "In the Romanian traditional medicine sage species are used as remedies for coughs,
rheumatism, inflammatory and bacterial diseases, as well as antidiabetic remedies.
In this study, an HPLC method was applied for determination of 22 phenolic
compounds in extracts of S. glutinosa, S. transsylvanica, and S. officinalis. The enzyme
inhibitory potential of the extracts was evaluated using microtiter assays, and
the antimicrobial potential was tested using the microdiluation assay for eight
microorganisms. These extracts were further tested on three different cancer cell lines
(A549, HepG2 and MCF-7) at increasing concentrations (1.56-200 μg/mL) for 24h/48h.
The chromatographic fingerprint revealed that among investigated compounds, the
dominant compounds of Salvia species are rutin (1357.9 - 4070.2 μg g-1) and catechin
(1112.6 - 1911.1 μg g-1). Concerning the enzyme inhibitory assays, both S. officinalis and
S. transsylvanica extracts exhibited an important inhibitory potential against alpha glucosidase (27.01 mmolACAE/g extract, and 25.62 mmolACAE/g extract, respectively).
The most sensitive bacteria to the extracts were Enterobacter cloacae (MIC = 0.01 mg/
mL, MBC = 0.02 mg/mL for S. officinalis) and Bacillus cereus (MIC = 0.09 mg/mL, MBC
= 0.18 mg/mL), while Penicillium funiculosum was the most sensitive fungal strain to
S. officinalis extract (MIC = 0.06 mg/mL, MFC = 0.12 mg/mL). From the three extracts,
the S. officinalis extract exhibited the most potent cytotoxic effect. Interestingly, when
testing on the estrogenic responsive cell line MCF-7, an increase in the viability was
observed for intermediary doses which we hypothesize to be related to the estrogen like compounds present in Salvia species.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "Phytochemical investigations of Salvia transsylvanica, Salvia glutinosa, and Salvia officinalis from Romania and their bioactivities",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4372"
}