TY  - JOUR
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Nilsson, Anders K.
AU  - Wulff, Angela
PY  - 2020
UR  - https://www.sciencedirect.com/science/article/pii/S0031942219304558?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3529
AB  - Desmids (Zygnematophyceae) are a group of poorly studied green microalgae. The aim of the present study was to identify fatty acids (FAs) that could be used as biomarkers in desmids in general, and to determine FAs as traits within different ecophysiological desmid groups. FA profiles of 29 desmid strains were determined and analysed with respect to their geographic origin, trophic preference and age of cultivation. It appeared that merely FAs present in relatively large proportions such as palmitic, linoleic, α-linolenic and hexadecatrienoic acids could be used as biomarkers for reliable categorization of this microalgal group. Linear discriminant analysis applied to three a priori defined groups of desmids, revealed clear strain-specific characteristics regarding FA distribution, influenced by climate and trophic conditions at the source sites as well as by the age of culture and growth phase. Accordingly, when considering FAs for the determination of lower taxonomic ranks we recommend using the term "trait" instead of "biomarker", as the latter designates unchangeable "fingerprint" of a specific taxon. Furthermore, despite that desmids were regarded as microalgae having stable genomes, long-term cultivation appeared to cause modifications in FA metabolic pathways, evident as a larger proportion of stearidonic acid in desmid strains cultivated over extensive time periods (>35 years).
T2  - Phytochemistry
T1  - Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.
VL  - 170
DO  - 10.1016/j.phytochem.2019.112200
SP  - 112200
ER  - 

@article{
author = "Stamenković, Marija and Steinwall, Elin and Nilsson, Anders K. and Wulff, Angela",
year = "2020",
abstract = "Desmids (Zygnematophyceae) are a group of poorly studied green microalgae. The aim of the present study was to identify fatty acids (FAs) that could be used as biomarkers in desmids in general, and to determine FAs as traits within different ecophysiological desmid groups. FA profiles of 29 desmid strains were determined and analysed with respect to their geographic origin, trophic preference and age of cultivation. It appeared that merely FAs present in relatively large proportions such as palmitic, linoleic, α-linolenic and hexadecatrienoic acids could be used as biomarkers for reliable categorization of this microalgal group. Linear discriminant analysis applied to three a priori defined groups of desmids, revealed clear strain-specific characteristics regarding FA distribution, influenced by climate and trophic conditions at the source sites as well as by the age of culture and growth phase. Accordingly, when considering FAs for the determination of lower taxonomic ranks we recommend using the term "trait" instead of "biomarker", as the latter designates unchangeable "fingerprint" of a specific taxon. Furthermore, despite that desmids were regarded as microalgae having stable genomes, long-term cultivation appeared to cause modifications in FA metabolic pathways, evident as a larger proportion of stearidonic acid in desmid strains cultivated over extensive time periods (>35 years).",
journal = "Phytochemistry",
title = "Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.",
volume = "170",
doi = "10.1016/j.phytochem.2019.112200",
pages = "112200"
}

Stamenković, M., Steinwall, E., Nilsson, A. K.,& Wulff, A.. (2020). Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.. in Phytochemistry, 170, 112200.
https://doi.org/10.1016/j.phytochem.2019.112200

Stamenković M, Steinwall E, Nilsson AK, Wulff A. Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach.. in Phytochemistry. 2020;170:112200.
doi:10.1016/j.phytochem.2019.112200 .

Stamenković, Marija, Steinwall, Elin, Nilsson, Anders K., Wulff, Angela, "Fatty acids as chemotaxonomic and ecophysiological traits in green microalgae (desmids, Zygnematophyceae, Streptophyta): A discriminant analysis approach." in Phytochemistry, 170 (2020):112200,
https://doi.org/10.1016/j.phytochem.2019.112200 . .

TY  - CONF
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Nilsson, Anders
AU  - Wulff, Angela
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6788
AB  - Microalgae have received a lot of attention as a source of high-lipid feedstock to be used for
the production of renewable energy in the form of biodiesel or other types of fuel, such as bioethanol,
biobutanol and biogas. In addition, many microalgal strains produce significant amounts
of essential fatty acids (such as ω3- and ω6-polyunsaturated fatty acids) which may have large
importance in nutrition, cosmetics, and pharmacy. Recent investigations demonstrated that a
group of freshwater green microalgae – desmids (Zygnematophyceae, Streptophyta) might have a
significant potential for the commercial fatty acid production. Over 20 desmid strains were grown
in standard cultivation conditions to assess their biomass and fatty acid content and productivities
during the growth phases. The desmid strains investigated demonstrated the moderate values of
biomass productivity (around 0.08 g CDW L-1 d-1), being the highest in Staurastrum boreale (0.14
g CDW L-1 d-1). The six desmid strains belonging to C. crenatum var. boldtianum, C. meneghinii, C.
regnellii var. pseudoregnellii, C. leave, Staurastrum boreale and S. punctulatum had the exceedingly
high total fatty acid contents (>200 mg g-1 CDW). The high amounts of linoleic and palmitic acids,
and the low amount of oleic acid influenced the characteristics of desmid biodiesel, which partly
satisfied the demanded standards for biodiesel. Hence, the improvement of the desmid biodiesel
is recommended by the enhancement of the desmid growth conditions and/or by the partial
hydrogenation. In addition, palmitic, linoleic and hexadecadienoic acids from desmid extracts can
be utilized in cosmetics, pharmacy, medicine, and in industry.
PB  - Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Strains of green microalgae as a promising feedstock for commercial fatty acid production
SP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6788
ER  - 

@conference{
author = "Stamenković, Marija and Steinwall, Elin and Nilsson, Anders and Wulff, Angela",
year = "2018",
abstract = "Microalgae have received a lot of attention as a source of high-lipid feedstock to be used for
the production of renewable energy in the form of biodiesel or other types of fuel, such as bioethanol,
biobutanol and biogas. In addition, many microalgal strains produce significant amounts
of essential fatty acids (such as ω3- and ω6-polyunsaturated fatty acids) which may have large
importance in nutrition, cosmetics, and pharmacy. Recent investigations demonstrated that a
group of freshwater green microalgae – desmids (Zygnematophyceae, Streptophyta) might have a
significant potential for the commercial fatty acid production. Over 20 desmid strains were grown
in standard cultivation conditions to assess their biomass and fatty acid content and productivities
during the growth phases. The desmid strains investigated demonstrated the moderate values of
biomass productivity (around 0.08 g CDW L-1 d-1), being the highest in Staurastrum boreale (0.14
g CDW L-1 d-1). The six desmid strains belonging to C. crenatum var. boldtianum, C. meneghinii, C.
regnellii var. pseudoregnellii, C. leave, Staurastrum boreale and S. punctulatum had the exceedingly
high total fatty acid contents (>200 mg g-1 CDW). The high amounts of linoleic and palmitic acids,
and the low amount of oleic acid influenced the characteristics of desmid biodiesel, which partly
satisfied the demanded standards for biodiesel. Hence, the improvement of the desmid biodiesel
is recommended by the enhancement of the desmid growth conditions and/or by the partial
hydrogenation. In addition, palmitic, linoleic and hexadecadienoic acids from desmid extracts can
be utilized in cosmetics, pharmacy, medicine, and in industry.",
publisher = "Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Strains of green microalgae as a promising feedstock for commercial fatty acid production",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6788"
}

Stamenković, M., Steinwall, E., Nilsson, A.,& Wulff, A.. (2018). Strains of green microalgae as a promising feedstock for commercial fatty acid production. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 148.
https://hdl.handle.net/21.15107/rcub_ibiss_6788

Stamenković M, Steinwall E, Nilsson A, Wulff A. Strains of green microalgae as a promising feedstock for commercial fatty acid production. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:148.
https://hdl.handle.net/21.15107/rcub_ibiss_6788 .

Stamenković, Marija, Steinwall, Elin, Nilsson, Anders, Wulff, Angela, "Strains of green microalgae as a promising feedstock for commercial fatty acid production" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):148,
https://hdl.handle.net/21.15107/rcub_ibiss_6788 .

TY  - CONF
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Nilsson, Anders K.
AU  - Wulff, Angela
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6787
AB  - Mikroalge su dobile veliki interes u istraživanju jer predstavljaju biomasu bogatu lipidima, koja može da se iskoristi kao dodatak ishrani i za proizvodnju biodizela. Dva soja dezmidnih mikroalgi, Staurastrum boreale No. 595 izolovan 1984. godine u Austriji i No. 631 izolovan 2014. sa lokaliteta u Srbiji, su uzgajana u standardnim laboratorijskim uslovima kako bi se procenile njihove fiziološke razlike (merenjem fotosinteze PAM fluorometrom, stope rasta i produkcije biomase), i da bi se odredio potencijal produkcije masnih kiselina gasnom hromatografijom. Istraživanje je pokazalo da noviji soj S. boreale ima veći fotosintetski prinos (Fv/Fm=0,71) u odnosu na stariji soj (Fv/Fm=0,679) kao i značajno veću maksimalnu stopu rasta (0,432 d-1, prema 0,311 d-1). Intenzivniji fotosintetski prinos je rezultovao u većoj biomasi novog soja S. boreale na kraju stacionarne faze rasta (1,55 g L-1), kao i u većoj produkciji biomase (0,14 g suve mase L-1 d-1), dok je stariji soj imao produkciju biomase svega 0,049 g suve mase L-1 d-1. Soj S. boreale izolovan sa lokaliteta u Srbiji se odlikovao izuzetno visokim sadržajem ukupnih masnih kiselina (231,5 mg g-1 suve mase) čime je tri puta prevazišao količinu određenu za stariji soj (73,8 mg g-1 suve mase). Uzimajući u obzir da je produkcija masnih kiselina takođe bila znatno veća kod novog soja S. boreale (8,2 mg L-1 d-1, naspram 0,69 mg L-1 d-1) ovaj soj se proporučuje za dalja istraživanja sa ciljem komercijalne produkcije algalnih lipida.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Fiziološke razlike između dva soja zelenih mikroalgi i njihov potencijal za produkciju masnih kiselina
SP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6787
ER  - 

@conference{
author = "Stamenković, Marija and Steinwall, Elin and Nilsson, Anders K. and Wulff, Angela",
year = "2018",
abstract = "Mikroalge su dobile veliki interes u istraživanju jer predstavljaju biomasu bogatu lipidima, koja može da se iskoristi kao dodatak ishrani i za proizvodnju biodizela. Dva soja dezmidnih mikroalgi, Staurastrum boreale No. 595 izolovan 1984. godine u Austriji i No. 631 izolovan 2014. sa lokaliteta u Srbiji, su uzgajana u standardnim laboratorijskim uslovima kako bi se procenile njihove fiziološke razlike (merenjem fotosinteze PAM fluorometrom, stope rasta i produkcije biomase), i da bi se odredio potencijal produkcije masnih kiselina gasnom hromatografijom. Istraživanje je pokazalo da noviji soj S. boreale ima veći fotosintetski prinos (Fv/Fm=0,71) u odnosu na stariji soj (Fv/Fm=0,679) kao i značajno veću maksimalnu stopu rasta (0,432 d-1, prema 0,311 d-1). Intenzivniji fotosintetski prinos je rezultovao u većoj biomasi novog soja S. boreale na kraju stacionarne faze rasta (1,55 g L-1), kao i u većoj produkciji biomase (0,14 g suve mase L-1 d-1), dok je stariji soj imao produkciju biomase svega 0,049 g suve mase L-1 d-1. Soj S. boreale izolovan sa lokaliteta u Srbiji se odlikovao izuzetno visokim sadržajem ukupnih masnih kiselina (231,5 mg g-1 suve mase) čime je tri puta prevazišao količinu određenu za stariji soj (73,8 mg g-1 suve mase). Uzimajući u obzir da je produkcija masnih kiselina takođe bila znatno veća kod novog soja S. boreale (8,2 mg L-1 d-1, naspram 0,69 mg L-1 d-1) ovaj soj se proporučuje za dalja istraživanja sa ciljem komercijalne produkcije algalnih lipida.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Fiziološke razlike između dva soja zelenih mikroalgi i njihov potencijal za produkciju masnih kiselina",
pages = "205",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6787"
}

Stamenković, M., Steinwall, E., Nilsson, A. K.,& Wulff, A.. (2018). Fiziološke razlike između dva soja zelenih mikroalgi i njihov potencijal za produkciju masnih kiselina. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Society., 205.
https://hdl.handle.net/21.15107/rcub_ibiss_6787

Stamenković M, Steinwall E, Nilsson AK, Wulff A. Fiziološke razlike između dva soja zelenih mikroalgi i njihov potencijal za produkciju masnih kiselina. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:205.
https://hdl.handle.net/21.15107/rcub_ibiss_6787 .

Stamenković, Marija, Steinwall, Elin, Nilsson, Anders K., Wulff, Angela, "Fiziološke razlike između dva soja zelenih mikroalgi i njihov potencijal za produkciju masnih kiselina" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):205,
https://hdl.handle.net/21.15107/rcub_ibiss_6787 .

TY  - JOUR
AU  - Stamenković, Marija
AU  - Steinwall, Elin
AU  - Nilsson, Anders K.
AU  - Wulff, Angela
PY  - 2018
UR  - http://link.springer.com/10.1007/s10811-018-1598-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3129
AB  - This study aimed to investigate fatty acid content and productivity of the insufficiently investigated group of freshwater microalgae—desmids (Zygnematophyceae, Streptophyta)—and to estimate their commercial potential. A total of 29 desmid strains of various environmental preferences were grown in standard cultivation conditions to assess fatty acid concentration and composition and biomass productivity during the growth phases. Six desmid strains belonging to Cosmarium crenatum var. boldtianum, C. meneghinii, C. regnellii var. pseudoregnellii, C. leave, Staurastrum boreale, and S. punctulatum had distinctly high total fatty acid contents (> 200 mg g−1 dry weight), among which C. crenatum had by far the highest average of total fatty acids (308.1 mg g−1 dry weight). Despite that desmids were grown in a medium which was not enriched with nutrients and CO2, these six strains achieved moderate biomass productivity (up to 0.14 g dry weight L−1 day−1), while the fatty acid productivity was in the range 8–11.1 mg L−1 day−1. The relatively high amounts of linoleic and palmitic acids in C. crenatum var. boldtianum and C. meneghinii were comparable to those found in several commercially grown plants, indicating that fatty acid extracts of these desmids could be utilized in cosmetics, pharmacy, medicine, or in additional industrial applications. On the other hand, the high proportion of oleic acid in a new isolate of Staurastrum boreale, along with its relatively high biomass productivity and cell size, pointed that this strain might be used for further investigations regarding biodiesel production.
T2  - Journal of Applied Phycology
T1  - Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study
DO  - 10.1007/s10811-018-1598-8
ER  - 

@article{
author = "Stamenković, Marija and Steinwall, Elin and Nilsson, Anders K. and Wulff, Angela",
year = "2018",
abstract = "This study aimed to investigate fatty acid content and productivity of the insufficiently investigated group of freshwater microalgae—desmids (Zygnematophyceae, Streptophyta)—and to estimate their commercial potential. A total of 29 desmid strains of various environmental preferences were grown in standard cultivation conditions to assess fatty acid concentration and composition and biomass productivity during the growth phases. Six desmid strains belonging to Cosmarium crenatum var. boldtianum, C. meneghinii, C. regnellii var. pseudoregnellii, C. leave, Staurastrum boreale, and S. punctulatum had distinctly high total fatty acid contents (> 200 mg g−1 dry weight), among which C. crenatum had by far the highest average of total fatty acids (308.1 mg g−1 dry weight). Despite that desmids were grown in a medium which was not enriched with nutrients and CO2, these six strains achieved moderate biomass productivity (up to 0.14 g dry weight L−1 day−1), while the fatty acid productivity was in the range 8–11.1 mg L−1 day−1. The relatively high amounts of linoleic and palmitic acids in C. crenatum var. boldtianum and C. meneghinii were comparable to those found in several commercially grown plants, indicating that fatty acid extracts of these desmids could be utilized in cosmetics, pharmacy, medicine, or in additional industrial applications. On the other hand, the high proportion of oleic acid in a new isolate of Staurastrum boreale, along with its relatively high biomass productivity and cell size, pointed that this strain might be used for further investigations regarding biodiesel production.",
journal = "Journal of Applied Phycology",
title = "Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study",
doi = "10.1007/s10811-018-1598-8"
}

Stamenković, M., Steinwall, E., Nilsson, A. K.,& Wulff, A.. (2018). Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study. in Journal of Applied Phycology.
https://doi.org/10.1007/s10811-018-1598-8

Stamenković M, Steinwall E, Nilsson AK, Wulff A. Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study. in Journal of Applied Phycology. 2018;.
doi:10.1007/s10811-018-1598-8 .

Stamenković, Marija, Steinwall, Elin, Nilsson, Anders K., Wulff, Angela, "Desmids (Zygnematophyceae, Streptophyta) as a promising freshwater microalgal group for the fatty acid production: results of a screening study" in Journal of Applied Phycology (2018),
https://doi.org/10.1007/s10811-018-1598-8 . .