TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Dovenska, Monika
AU  - Ajdžanović, Vladimir
AU  - Marković Filipović, Jelena
AU  - Ugrenović, Slađana
AU  - Kundalić, Braca
AU  - Milošević, Verica
AU  - Popovska-Perčinić, Florina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6278
AB  - Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6278
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Dovenska, Monika and Ajdžanović, Vladimir and Marković Filipović, Jelena and Ugrenović, Slađana and Kundalić, Braca and Milošević, Verica and Popovska-Perčinić, Florina",
year = "2023",
abstract = "Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6278"
}

Čukuranović-Kokoris, J., Dovenska, M., Ajdžanović, V., Marković Filipović, J., Ugrenović, S., Kundalić, B., Milošević, V.,& Popovska-Perčinić, F.. (2023). Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278

Čukuranović-Kokoris J, Dovenska M, Ajdžanović V, Marković Filipović J, Ugrenović S, Kundalić B, Milošević V, Popovska-Perčinić F. Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

Čukuranović-Kokoris, Jovana, Dovenska, Monika, Ajdžanović, Vladimir, Marković Filipović, Jelena, Ugrenović, Slađana, Kundalić, Braca, Milošević, Verica, Popovska-Perčinić, Florina, "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):160,
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Jovanović, Ivan
AU  - Pantović, Vukica
AU  - Krstić, Miljan
AU  - Stanojković, Milica
AU  - Milošević, Verica
AU  - Ugrenović, Slađana
AU  - Stojanović, Vesna
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0040816616302464
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27884532
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2684
AB  - The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years. Volume density of the LH cells did not significantly change, whereas their area significantly increased with age. Nucleocytoplasmic ratio of the LH cells gradually decreased and became significant after the age of 70. Finally, volume density of the FS cell significantly correlated with LH cells area and nucleocytoplasmic ratio. From all above cited, we concluded that in men, density and size of the FS cells increase with age. Long-term hypertrophy of the LH cells results in their functional decline after the age of 70. Strong correlation between FS cells and LH cells morphometric parameters might point to age-related interaction between these two cell groups.
T2  - Tissue & Cell
T1  - Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process.
IS  - 1
VL  - 49
DO  - 10.1016/j.tice.2016.11.006
SP  - 78
EP  - 85
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Jovanović, Ivan and Pantović, Vukica and Krstić, Miljan and Stanojković, Milica and Milošević, Verica and Ugrenović, Slađana and Stojanović, Vesna",
year = "2017",
abstract = "The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years. Volume density of the LH cells did not significantly change, whereas their area significantly increased with age. Nucleocytoplasmic ratio of the LH cells gradually decreased and became significant after the age of 70. Finally, volume density of the FS cell significantly correlated with LH cells area and nucleocytoplasmic ratio. From all above cited, we concluded that in men, density and size of the FS cells increase with age. Long-term hypertrophy of the LH cells results in their functional decline after the age of 70. Strong correlation between FS cells and LH cells morphometric parameters might point to age-related interaction between these two cell groups.",
journal = "Tissue & Cell",
title = "Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process.",
number = "1",
volume = "49",
doi = "10.1016/j.tice.2016.11.006",
pages = "78-85"
}

Čukuranović Kokoris, J., Jovanović, I., Pantović, V., Krstić, M., Stanojković, M., Milošević, V., Ugrenović, S.,& Stojanović, V.. (2017). Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process.. in Tissue & Cell, 49(1), 78-85.
https://doi.org/10.1016/j.tice.2016.11.006

Čukuranović Kokoris J, Jovanović I, Pantović V, Krstić M, Stanojković M, Milošević V, Ugrenović S, Stojanović V. Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process.. in Tissue & Cell. 2017;49(1):78-85.
doi:10.1016/j.tice.2016.11.006 .

Čukuranović Kokoris, Jovana, Jovanović, Ivan, Pantović, Vukica, Krstić, Miljan, Stanojković, Milica, Milošević, Verica, Ugrenović, Slađana, Stojanović, Vesna, "Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process." in Tissue & Cell, 49, no. 1 (2017):78-85,
https://doi.org/10.1016/j.tice.2016.11.006 . .