TY  - JOUR
AU  - Neto, Iris
AU  - Domínguez-Martín, Eva María
AU  - Ntungwe, Epole
AU  - Reis, Catarina
AU  - Pešić, Milica
AU  - Faustino, Célia
AU  - Rijo, Patrícia
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4262
AB  - The antimicrobial activity of dehydroabietic acid (DHA) for its use as an antibiofilm agent was tested in this work. DHA was assayed against a collection of Gram-positive, Gram-negative sensitive and resistant bacteria and yeasts through the minimum inhibitory concentration (MIC), MIC with Bioburden challenge, minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), MBIC with Bioburden challenge and growth curve studies. Toxicological studies (Artemia salina, sulforhodamine B (SRB) assay) were done to assess if the compound had antimicrobial and not cytotoxic properties. Furthermore, microencapsulation and stability studies were carried out to evaluate the chemical behavior and stability of DHA. On MIC results, Gram-positive bacteria Staphylococcus aureus ATCC 1228 and Mycobacterium smegmatis ATCC 607 presented a high efficiency (7.81 µg/mL), while on Gram-negative bacteria the highest MIC value of 125 µg/mL was obtained by all Klebsiella pneumoniae strains and Escherichia coli isolate strain HSM 303. Bioburden challenge showed that MIC, MBIC and percentage biofilm inhibition (BI) values suffered alterations, therefore, having higher concentrations. MBIC values demonstrated that DHA has a higher efficiency against S. aureus ATCC 43866 with a percentage of BI of 75.13 ± 0.82% at 0.49 µg/mL. Growth curve kinetic profiles of DHA against S. aureus ATCC 25923 were observed to be bacteriostatic. DHA-alginate beads had a average size of 2.37 ± 0.20 and 2.31 ± 0.17 × 103 µm2 with an encapsulation efficiency (EE%) around 99.49 ± 0.05%, a protection percentage (PP%) of 60.00 ± 0.05% in the gastric environment and a protection efficiency (PE%) around 88.12 ± 0.05% against UV light. In toxicological studies DHA has shown IC50 of 19.59 ± 7.40 µg/mL and a LC50 of 21.71 ± 2.18%. The obtained results indicate that DHA is a promising antimicrobial candidate against a wide range of bacteria and biofilm formation that must be further explored.
PB  - Basel : MDPI
T2  - Pharmaceutics
T1  - Dehydroabietic Acid Microencapsulation Potential as Biofilm-Mediated Infections Treatment
IS  - 6
VL  - 13
DO  - 10.3390/pharmaceutics13060825
SP  - 825
ER  - 

@article{
author = "Neto, Iris and Domínguez-Martín, Eva María and Ntungwe, Epole and Reis, Catarina and Pešić, Milica and Faustino, Célia and Rijo, Patrícia",
year = "2021",
abstract = "The antimicrobial activity of dehydroabietic acid (DHA) for its use as an antibiofilm agent was tested in this work. DHA was assayed against a collection of Gram-positive, Gram-negative sensitive and resistant bacteria and yeasts through the minimum inhibitory concentration (MIC), MIC with Bioburden challenge, minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), MBIC with Bioburden challenge and growth curve studies. Toxicological studies (Artemia salina, sulforhodamine B (SRB) assay) were done to assess if the compound had antimicrobial and not cytotoxic properties. Furthermore, microencapsulation and stability studies were carried out to evaluate the chemical behavior and stability of DHA. On MIC results, Gram-positive bacteria Staphylococcus aureus ATCC 1228 and Mycobacterium smegmatis ATCC 607 presented a high efficiency (7.81 µg/mL), while on Gram-negative bacteria the highest MIC value of 125 µg/mL was obtained by all Klebsiella pneumoniae strains and Escherichia coli isolate strain HSM 303. Bioburden challenge showed that MIC, MBIC and percentage biofilm inhibition (BI) values suffered alterations, therefore, having higher concentrations. MBIC values demonstrated that DHA has a higher efficiency against S. aureus ATCC 43866 with a percentage of BI of 75.13 ± 0.82% at 0.49 µg/mL. Growth curve kinetic profiles of DHA against S. aureus ATCC 25923 were observed to be bacteriostatic. DHA-alginate beads had a average size of 2.37 ± 0.20 and 2.31 ± 0.17 × 103 µm2 with an encapsulation efficiency (EE%) around 99.49 ± 0.05%, a protection percentage (PP%) of 60.00 ± 0.05% in the gastric environment and a protection efficiency (PE%) around 88.12 ± 0.05% against UV light. In toxicological studies DHA has shown IC50 of 19.59 ± 7.40 µg/mL and a LC50 of 21.71 ± 2.18%. The obtained results indicate that DHA is a promising antimicrobial candidate against a wide range of bacteria and biofilm formation that must be further explored.",
publisher = "Basel : MDPI",
journal = "Pharmaceutics",
title = "Dehydroabietic Acid Microencapsulation Potential as Biofilm-Mediated Infections Treatment",
number = "6",
volume = "13",
doi = "10.3390/pharmaceutics13060825",
pages = "825"
}

Neto, I., Domínguez-Martín, E. M., Ntungwe, E., Reis, C., Pešić, M., Faustino, C.,& Rijo, P.. (2021). Dehydroabietic Acid Microencapsulation Potential as Biofilm-Mediated Infections Treatment. in Pharmaceutics
Basel : MDPI., 13(6), 825.
https://doi.org/10.3390/pharmaceutics13060825

Neto I, Domínguez-Martín EM, Ntungwe E, Reis C, Pešić M, Faustino C, Rijo P. Dehydroabietic Acid Microencapsulation Potential as Biofilm-Mediated Infections Treatment. in Pharmaceutics. 2021;13(6):825.
doi:10.3390/pharmaceutics13060825 .

Neto, Iris, Domínguez-Martín, Eva María, Ntungwe, Epole, Reis, Catarina, Pešić, Milica, Faustino, Célia, Rijo, Patrícia, "Dehydroabietic Acid Microencapsulation Potential as Biofilm-Mediated Infections Treatment" in Pharmaceutics, 13, no. 6 (2021):825,
https://doi.org/10.3390/pharmaceutics13060825 . .

TY  - JOUR
AU  - Matias, Diogo
AU  - Nicolai, Marisa
AU  - Saraiva, Lucília
AU  - Pinheiro, Rute
AU  - Faustino, Célia
AU  - Diaz Lanza, Ana
AU  - Pinto Reis, Catarina
AU  - Stanković, Tijana
AU  - Dinić, Jelena
AU  - Pešić, Milica
AU  - Rijo, Patrícia
PY  - 2019
UR  - http://pubs.acs.org/doi/10.1021/acsomega.9b00512
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3349
AB  - Cytotoxicity screenings have identified Plectranthus plants as potential sources of antitumor lead compounds. In this work, several extracts from Plectranthus madagascariensis were prepared using different solvents (acetone, methanol, and supercritical CO2) and extraction techniques (maceration, ultrasound-assisted, and supercritical fluid extraction), and their chemical composition was detailed using high-performance liquid chromatography with a diode array detector. The cytotoxic activity of the major compounds identified, namely, rosmarinic acid (1) and abietane diterpenes 7α,6β-dihydroxyroyleanone (2), 7α-formyloxy-6β-hydroxyroyleanone (3), 7α-acetoxy-6β-hydroxyroyleanone (4), and coleon U (5), was evaluated in a battery of human cancer cell lines, including breast (MDA-MB-231, MCF-7), colon (HCT116), and lung (NCI-H460, NCI-H460/R) cancer, and also in healthy lung (MCR-5) cells. Royleanone (3) was isolated for the first time from P. madagascariensis, and its full spectroscopic characterization (proto...
T2  - ACS Omega
T1  - Cytotoxic Activity of Royleanone Diterpenes from <i>Plectranthus madagascariensis</i> Benth
IS  - 5
VL  - 4
DO  - 10.1021/acsomega.9b00512
SP  - 8094
EP  - 8103
ER  - 

@article{
author = "Matias, Diogo and Nicolai, Marisa and Saraiva, Lucília and Pinheiro, Rute and Faustino, Célia and Diaz Lanza, Ana and Pinto Reis, Catarina and Stanković, Tijana and Dinić, Jelena and Pešić, Milica and Rijo, Patrícia",
year = "2019",
abstract = "Cytotoxicity screenings have identified Plectranthus plants as potential sources of antitumor lead compounds. In this work, several extracts from Plectranthus madagascariensis were prepared using different solvents (acetone, methanol, and supercritical CO2) and extraction techniques (maceration, ultrasound-assisted, and supercritical fluid extraction), and their chemical composition was detailed using high-performance liquid chromatography with a diode array detector. The cytotoxic activity of the major compounds identified, namely, rosmarinic acid (1) and abietane diterpenes 7α,6β-dihydroxyroyleanone (2), 7α-formyloxy-6β-hydroxyroyleanone (3), 7α-acetoxy-6β-hydroxyroyleanone (4), and coleon U (5), was evaluated in a battery of human cancer cell lines, including breast (MDA-MB-231, MCF-7), colon (HCT116), and lung (NCI-H460, NCI-H460/R) cancer, and also in healthy lung (MCR-5) cells. Royleanone (3) was isolated for the first time from P. madagascariensis, and its full spectroscopic characterization (proto...",
journal = "ACS Omega",
title = "Cytotoxic Activity of Royleanone Diterpenes from <i>Plectranthus madagascariensis</i> Benth",
number = "5",
volume = "4",
doi = "10.1021/acsomega.9b00512",
pages = "8094-8103"
}

Matias, D., Nicolai, M., Saraiva, L., Pinheiro, R., Faustino, C., Diaz Lanza, A., Pinto Reis, C., Stanković, T., Dinić, J., Pešić, M.,& Rijo, P.. (2019). Cytotoxic Activity of Royleanone Diterpenes from <i>Plectranthus madagascariensis</i> Benth. in ACS Omega, 4(5), 8094-8103.
https://doi.org/10.1021/acsomega.9b00512

Matias D, Nicolai M, Saraiva L, Pinheiro R, Faustino C, Diaz Lanza A, Pinto Reis C, Stanković T, Dinić J, Pešić M, Rijo P. Cytotoxic Activity of Royleanone Diterpenes from <i>Plectranthus madagascariensis</i> Benth. in ACS Omega. 2019;4(5):8094-8103.
doi:10.1021/acsomega.9b00512 .

Matias, Diogo, Nicolai, Marisa, Saraiva, Lucília, Pinheiro, Rute, Faustino, Célia, Diaz Lanza, Ana, Pinto Reis, Catarina, Stanković, Tijana, Dinić, Jelena, Pešić, Milica, Rijo, Patrícia, "Cytotoxic Activity of Royleanone Diterpenes from <i>Plectranthus madagascariensis</i> Benth" in ACS Omega, 4, no. 5 (2019):8094-8103,
https://doi.org/10.1021/acsomega.9b00512 . .