TY  - JOUR
AU  - Nestorović, Nataša
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Ristić, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Manojlović-Stojanoski, Milica
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/32
AB  - Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH- and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH- and LH-immunopositive cells per unit area (mm2) was significantly reduced. Octreotide also induced a significant reduction of the FSH- and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells.
AB  - Ispitivani su efekti oktreotida davanog intracerebroventrikularno (ICV) odraslim ženkama pacova na gonadotropne ćelije (FSH i LH). Životinje su tretirane sa ukupno tri doze od 1 mg oktreotida rastvorenog u 10 mL fiziološkog rastvora svakog drugog dana. Kontrole su na isti način primile odgovarajuću zapreminu fiziološ kog rastvora. FSH i LH ćelije su ispitivane imunocitohemijskim postupkom peroksidaza-antiperoksidaza. Da bi se ustanovile promene u broju, zapremini i zapreminskoj gustini gonadotropnih ćelija primenjen je morfometrijski kao i stereološki pristup. Kod ženki tretiranih oktreotidom, gonadotropne ćelije su bile manje i često piknotič ne. Broj imunopozitivnih FSH i LH ćelija po jedinici površine (mm2) je bio statistički značajno smanjen. Oktreotid je, takodje, izazvao značajno smanjenje zapremine FSH i LH imunoreaktivnih ćelija, kao i njihove zapreminske gustine. Na osnovu ovih rezultata, može se zaključiti da je oktreotid, dat ICV odraslim ženkama pacova doveo do promena imunocitohemijskih i morfometrijskih osobina oba ispitivana tipa gonadotropnih ćelija.
PB  - Society of Medical Biochemists of Serbia
T2  - Jugoslovenska medicinska biohemija
T1  - Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova
T1  - Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats
IS  - 2
VL  - 23
DO  - 10.2298/JMH0402149N
SP  - 149
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_32
ER  - 

@article{
author = "Nestorović, Nataša and Lovren, Mirjana B. and Sekulić, Milka I. and Ristić, Nataša and Šošić-Jurjević, Branka  and Manojlović-Stojanoski, Milica and Filipović, Branko and Milošević, Verica",
year = "2004",
abstract = "Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH- and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH- and LH-immunopositive cells per unit area (mm2) was significantly reduced. Octreotide also induced a significant reduction of the FSH- and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells., Ispitivani su efekti oktreotida davanog intracerebroventrikularno (ICV) odraslim ženkama pacova na gonadotropne ćelije (FSH i LH). Životinje su tretirane sa ukupno tri doze od 1 mg oktreotida rastvorenog u 10 mL fiziološkog rastvora svakog drugog dana. Kontrole su na isti način primile odgovarajuću zapreminu fiziološ kog rastvora. FSH i LH ćelije su ispitivane imunocitohemijskim postupkom peroksidaza-antiperoksidaza. Da bi se ustanovile promene u broju, zapremini i zapreminskoj gustini gonadotropnih ćelija primenjen je morfometrijski kao i stereološki pristup. Kod ženki tretiranih oktreotidom, gonadotropne ćelije su bile manje i često piknotič ne. Broj imunopozitivnih FSH i LH ćelija po jedinici površine (mm2) je bio statistički značajno smanjen. Oktreotid je, takodje, izazvao značajno smanjenje zapremine FSH i LH imunoreaktivnih ćelija, kao i njihove zapreminske gustine. Na osnovu ovih rezultata, može se zaključiti da je oktreotid, dat ICV odraslim ženkama pacova doveo do promena imunocitohemijskih i morfometrijskih osobina oba ispitivana tipa gonadotropnih ćelija.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Jugoslovenska medicinska biohemija",
title = "Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova, Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats",
number = "2",
volume = "23",
doi = "10.2298/JMH0402149N",
pages = "149-153",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_32"
}

Nestorović, N., Lovren, M. B., Sekulić, M. I., Ristić, N., Šošić-Jurjević, B., Manojlović-Stojanoski, M., Filipović, B.,& Milošević, V.. (2004). Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova. in Jugoslovenska medicinska biohemija
Society of Medical Biochemists of Serbia., 23(2), 149-153.
https://doi.org/10.2298/JMH0402149N
https://hdl.handle.net/21.15107/rcub_ibiss_32

Nestorović N, Lovren MB, Sekulić MI, Ristić N, Šošić-Jurjević B, Manojlović-Stojanoski M, Filipović B, Milošević V. Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova. in Jugoslovenska medicinska biohemija. 2004;23(2):149-153.
doi:10.2298/JMH0402149N
https://hdl.handle.net/21.15107/rcub_ibiss_32 .

Nestorović, Nataša, Lovren, Mirjana B., Sekulić, Milka I., Ristić, Nataša, Šošić-Jurjević, Branka , Manojlović-Stojanoski, Milica, Filipović, Branko, Milošević, Verica, "Efekti intracerebroventrikularnog tretmana oktreotidom na gonadotropne ćelije ženki pacova" in Jugoslovenska medicinska biohemija, 23, no. 2 (2004):149-153,
https://doi.org/10.2298/JMH0402149N .,
https://hdl.handle.net/21.15107/rcub_ibiss_32 .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Nestorović, Nataša
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1792
AB  - The subject of these studies was the thyroid gland tissue of middle-aged (14-month-old) female rats chronically treated with calcium glucoheptonate. The peripheral and central zone of the thyroids were stereologically analysed and the following morphometric parameters determined: the height and volumetric density of follicular epithelium, colloid, interstitium and follicles and index of activation rate. The height of follicular epithelium, its volume density and index of activation rate were significantly reduced (by 8%, p < 0.05, 18%, p < 0.025 and 34%, p < 0.01, respectively) as compared to the controls. However, the volumetric density of colloid and interstitium were increased (by 10% and 14% respectively). These morphometric results indicate that Ca treatment expressed an inhibitory effect on thyroid follicular cells structure in middle-aged female rats.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Effect of calcium on structural and morphometric features of thyroid gland tissue in middle-aged rat females
IS  - 3
VL  - 21
DO  - 10.2298/JMH0203261S
SP  - 261
EP  - 267
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1792
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Nestorović, Nataša and Lovren, Mirjana B. and Sekulić, Milka I.",
year = "2002",
abstract = "The subject of these studies was the thyroid gland tissue of middle-aged (14-month-old) female rats chronically treated with calcium glucoheptonate. The peripheral and central zone of the thyroids were stereologically analysed and the following morphometric parameters determined: the height and volumetric density of follicular epithelium, colloid, interstitium and follicles and index of activation rate. The height of follicular epithelium, its volume density and index of activation rate were significantly reduced (by 8%, p < 0.05, 18%, p < 0.025 and 34%, p < 0.01, respectively) as compared to the controls. However, the volumetric density of colloid and interstitium were increased (by 10% and 14% respectively). These morphometric results indicate that Ca treatment expressed an inhibitory effect on thyroid follicular cells structure in middle-aged female rats.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Effect of calcium on structural and morphometric features of thyroid gland tissue in middle-aged rat females",
number = "3",
volume = "21",
doi = "10.2298/JMH0203261S",
pages = "261-267",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1792"
}

Šošić-Jurjević, B., Filipović, B., Nestorović, N., Lovren, M. B.,& Sekulić, M. I.. (2002). Effect of calcium on structural and morphometric features of thyroid gland tissue in middle-aged rat females. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 21(3), 261-267.
https://doi.org/10.2298/JMH0203261S
https://hdl.handle.net/21.15107/rcub_ibiss_1792

Šošić-Jurjević B, Filipović B, Nestorović N, Lovren MB, Sekulić MI. Effect of calcium on structural and morphometric features of thyroid gland tissue in middle-aged rat females. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 2002;21(3):261-267.
doi:10.2298/JMH0203261S
https://hdl.handle.net/21.15107/rcub_ibiss_1792 .

Šošić-Jurjević, Branka , Filipović, Branko, Nestorović, Nataša, Lovren, Mirjana B., Sekulić, Milka I., "Effect of calcium on structural and morphometric features of thyroid gland tissue in middle-aged rat females" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 21, no. 3 (2002):261-267,
https://doi.org/10.2298/JMH0203261S .,
https://hdl.handle.net/21.15107/rcub_ibiss_1792 .

TY  - JOUR
AU  - Nestorović, Nataša
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1809
AB  - The effect of multiple somatostatin (SRIH-14) treatment on the pituitary gonadotrophs, follicle stimulating harmone (FSH) and luteinizing harmone (LH), and ovaries of adult female Wistar rats was examined. Females received two 20 mug/100 g body wt. doses daily subcutaneously, for five consecutive days. FSH and LH cells were studied using a peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate changes in the number per unit area (mm(2)), cell volume and volume densities of LH- and FSH-immunoreactive cells. Ovaries were analysed by simple point counting of follicles and corpora lutea. Follicles were divided by size according to the classification of Gaytan and Osman. Morphometric and stereological analysis of the pituitary showed that the number, volume and the volume density of FSH- and LH-immunoreactive cells were decreased after multiple SRIH-14 treatment, particularly in the latter. In the ovary, SRIH-14 induced decreases in the number of healthy follicles in all phases of folliculogenesis and corpora lutea, but the large antral follicle stage was most affected. The number of atretic follicles was increased. It can be concluded that multiple SRIH-14 treatment markedly inhibited LH cells, but affected FSH cells as well. In the ovary, SRIH-14 acted by inhibiting folliculogenesis and enhancing atretic processes.
T2  - Histochemical Journal
T1  - Effects of multiple somatostatin treatment on rat gonadotrophic cells and ovaries
IS  - 11-12
VL  - 33
EP  - 702
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1809
ER  - 

@article{
author = "Nestorović, Nataša and Lovren, Mirjana B. and Sekulić, Milka I. and Filipović, Branko and Milošević, Verica",
year = "2001",
abstract = "The effect of multiple somatostatin (SRIH-14) treatment on the pituitary gonadotrophs, follicle stimulating harmone (FSH) and luteinizing harmone (LH), and ovaries of adult female Wistar rats was examined. Females received two 20 mug/100 g body wt. doses daily subcutaneously, for five consecutive days. FSH and LH cells were studied using a peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate changes in the number per unit area (mm(2)), cell volume and volume densities of LH- and FSH-immunoreactive cells. Ovaries were analysed by simple point counting of follicles and corpora lutea. Follicles were divided by size according to the classification of Gaytan and Osman. Morphometric and stereological analysis of the pituitary showed that the number, volume and the volume density of FSH- and LH-immunoreactive cells were decreased after multiple SRIH-14 treatment, particularly in the latter. In the ovary, SRIH-14 induced decreases in the number of healthy follicles in all phases of folliculogenesis and corpora lutea, but the large antral follicle stage was most affected. The number of atretic follicles was increased. It can be concluded that multiple SRIH-14 treatment markedly inhibited LH cells, but affected FSH cells as well. In the ovary, SRIH-14 acted by inhibiting folliculogenesis and enhancing atretic processes.",
journal = "Histochemical Journal",
title = "Effects of multiple somatostatin treatment on rat gonadotrophic cells and ovaries",
number = "11-12",
volume = "33",
pages = "702",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1809"
}

Nestorović, N., Lovren, M. B., Sekulić, M. I., Filipović, B.,& Milošević, V.. (2001). Effects of multiple somatostatin treatment on rat gonadotrophic cells and ovaries. in Histochemical Journal, 33(11-12).
https://hdl.handle.net/21.15107/rcub_ibiss_1809

Nestorović N, Lovren MB, Sekulić MI, Filipović B, Milošević V. Effects of multiple somatostatin treatment on rat gonadotrophic cells and ovaries. in Histochemical Journal. 2001;33(11-12):null-702.
https://hdl.handle.net/21.15107/rcub_ibiss_1809 .

Nestorović, Nataša, Lovren, Mirjana B., Sekulić, Milka I., Filipović, Branko, Milošević, Verica, "Effects of multiple somatostatin treatment on rat gonadotrophic cells and ovaries" in Histochemical Journal, 33, no. 11-12 (2001),
https://hdl.handle.net/21.15107/rcub_ibiss_1809 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1832
AB  - The results obtained during the studies on the effects of gonadal steroids on thyroid follicular cells (FC) of rats and pigs are summarized. The influence of a single (mg/animal) or double dose of estradiol-dipropionate (EDP) administered in different phases of male and female rat development was studied using cyto logical and stereological methods. A particular attention was paid to a characteristic inhibitory changes of thyroid FC of aged rats chronically treated with EDP and testosterone-propionate (TP). Chronic treatment of male rats, and the response of thyroid FC to hydrocortisone (Hy) was the object our investigations. Attention was focussed also on the specific changes in thyroid FC of newborn piglets treated with EDP and progesterone (Pr) in combination. The results revealed an inhibitory action of steroids on rat thyroid FC. In contrast, an increased activity of FC, after sex steroids treatment of piglets was observed.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Sensitivity of thyroid follicular cells to steroid hormones
IS  - 2
VL  - 19
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1832
ER  - 

@article{
author = "Sekulić, Milka I. and Lovren, Mirjana B.",
year = "2000",
abstract = "The results obtained during the studies on the effects of gonadal steroids on thyroid follicular cells (FC) of rats and pigs are summarized. The influence of a single (mg/animal) or double dose of estradiol-dipropionate (EDP) administered in different phases of male and female rat development was studied using cyto logical and stereological methods. A particular attention was paid to a characteristic inhibitory changes of thyroid FC of aged rats chronically treated with EDP and testosterone-propionate (TP). Chronic treatment of male rats, and the response of thyroid FC to hydrocortisone (Hy) was the object our investigations. Attention was focussed also on the specific changes in thyroid FC of newborn piglets treated with EDP and progesterone (Pr) in combination. The results revealed an inhibitory action of steroids on rat thyroid FC. In contrast, an increased activity of FC, after sex steroids treatment of piglets was observed.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Sensitivity of thyroid follicular cells to steroid hormones",
number = "2",
volume = "19",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1832"
}

Sekulić, M. I.,& Lovren, M. B.. (2000). Sensitivity of thyroid follicular cells to steroid hormones. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 19(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1832

Sekulić MI, Lovren MB. Sensitivity of thyroid follicular cells to steroid hormones. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 2000;19(2):null-85.
https://hdl.handle.net/21.15107/rcub_ibiss_1832 .

Sekulić, Milka I., Lovren, Mirjana B., "Sensitivity of thyroid follicular cells to steroid hormones" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 19, no. 2 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1832 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1830
AB  - The role of steroids in the regulation of thyroid C and mast cells function of rats and pigs were the object of our experimental examinations. The argyrophil properties, morphometric characteristics and subcellular organization of C and mast cells in rats of both sexes after neonatal estradiol-dipropionate treatment were studied. A special attention has been paid to the changes in the structure of thyroid C cells after a chronical treatment of aged (2-year-old) rats with estradiol-dipropionate or testosterone propionate. Besides, the structure and function of immunoreactive C cells of middle-aged female rats (14-month-old) chronically treated with estradiol-dipropionate, calcium or a combination of estradiol-dipropionate and calcium were observed. Serum levels of calcitonin, osteocalcin, parathormone and beta-estradiol were also measured using RLA method. It has been observed that neonatal estradiol-dipropionate treatment expresses a long-term stimulative effects on rat thyroid C cells, while chronic treatment of middle-aged rats with estradiol-dipropionate acts inhibitory on these cells. In addition, the argyrophil properties and ultrastructural characteristics of C cells of male pigs treated with estradiol--dipropionate and progesterone in combination or castrated on the first day of life and sacrificed 6 or 8 months later were investigated. A slower rate of specific protein synthesis and secretion of porcine C cells after sex steroids treatment was observed.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Responsiveness of thyroid C cells to steroid hormones
IS  - 3
VL  - 19
EP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1830
ER  - 

@article{
author = "Sekulić, Milka I. and Lovren, Mirjana B.",
year = "2000",
abstract = "The role of steroids in the regulation of thyroid C and mast cells function of rats and pigs were the object of our experimental examinations. The argyrophil properties, morphometric characteristics and subcellular organization of C and mast cells in rats of both sexes after neonatal estradiol-dipropionate treatment were studied. A special attention has been paid to the changes in the structure of thyroid C cells after a chronical treatment of aged (2-year-old) rats with estradiol-dipropionate or testosterone propionate. Besides, the structure and function of immunoreactive C cells of middle-aged female rats (14-month-old) chronically treated with estradiol-dipropionate, calcium or a combination of estradiol-dipropionate and calcium were observed. Serum levels of calcitonin, osteocalcin, parathormone and beta-estradiol were also measured using RLA method. It has been observed that neonatal estradiol-dipropionate treatment expresses a long-term stimulative effects on rat thyroid C cells, while chronic treatment of middle-aged rats with estradiol-dipropionate acts inhibitory on these cells. In addition, the argyrophil properties and ultrastructural characteristics of C cells of male pigs treated with estradiol--dipropionate and progesterone in combination or castrated on the first day of life and sacrificed 6 or 8 months later were investigated. A slower rate of specific protein synthesis and secretion of porcine C cells after sex steroids treatment was observed.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Responsiveness of thyroid C cells to steroid hormones",
number = "3",
volume = "19",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1830"
}

Sekulić, M. I.,& Lovren, M. B.. (2000). Responsiveness of thyroid C cells to steroid hormones. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 19(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1830

Sekulić MI, Lovren MB. Responsiveness of thyroid C cells to steroid hormones. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 2000;19(3):null-160.
https://hdl.handle.net/21.15107/rcub_ibiss_1830 .

Sekulić, Milka I., Lovren, Mirjana B., "Responsiveness of thyroid C cells to steroid hormones" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 19, no. 3 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1830 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Sekulić, Milka I.
AU  - Brkić, Branislava
AU  - Lovren, Mirjana B.
AU  - Starčević, Vesna P.
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1829
AB  - The effects of intracerebroventricularly administered somatostatin (SRIH-14 or -28) on growth and function of pituitary thyrotropes (TSH-cells) were examined in adult male Wistar rats. The animals were implanted with an intracerebroventricular cannula and after a recovery period, administered three 1 mug doses of SRIH-14 or -28 dissolved in 5 mul saline every second day. Controls were treated in the same way with the same volume of saline only. TSH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. Blood samples were collected for hormone (TSH) analyses 5 days after the last injection. Both SRIH-treatments significantly decreased (p < 0.05) all morphometric parameters obtained for TSH-cells in comparison with controls. The volume of TSH-cells decreased by 27%, nuclei by 44% and volume density by 33% in animals treated with SRIH-14. In animals treated with SRIH-28, these parameters were also significantly decreased (p < 0.05) (22%, 31%, and 25% respectively) compared to control rats. Serum concentrations of TSH were significantly decreased (p < 0.05) by 15% in SRIH-14- and by 12% in SRIH-28-treated animals in comparison with the controls. These observations suggest that centrally administered SRIH-14 or -28 is specifically involved in the control of growth and secretory activity of TSH cells.
T2  - Histochemical Journal
T1  - Effect of centrally administered somatostatin on pituitary thyrotropes in male rats
IS  - 9
VL  - 32
EP  - 569
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1829
ER  - 

@article{
author = "Milošević, Verica and Sekulić, Milka I. and Brkić, Branislava and Lovren, Mirjana B. and Starčević, Vesna P.",
year = "2000",
abstract = "The effects of intracerebroventricularly administered somatostatin (SRIH-14 or -28) on growth and function of pituitary thyrotropes (TSH-cells) were examined in adult male Wistar rats. The animals were implanted with an intracerebroventricular cannula and after a recovery period, administered three 1 mug doses of SRIH-14 or -28 dissolved in 5 mul saline every second day. Controls were treated in the same way with the same volume of saline only. TSH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. Blood samples were collected for hormone (TSH) analyses 5 days after the last injection. Both SRIH-treatments significantly decreased (p < 0.05) all morphometric parameters obtained for TSH-cells in comparison with controls. The volume of TSH-cells decreased by 27%, nuclei by 44% and volume density by 33% in animals treated with SRIH-14. In animals treated with SRIH-28, these parameters were also significantly decreased (p < 0.05) (22%, 31%, and 25% respectively) compared to control rats. Serum concentrations of TSH were significantly decreased (p < 0.05) by 15% in SRIH-14- and by 12% in SRIH-28-treated animals in comparison with the controls. These observations suggest that centrally administered SRIH-14 or -28 is specifically involved in the control of growth and secretory activity of TSH cells.",
journal = "Histochemical Journal",
title = "Effect of centrally administered somatostatin on pituitary thyrotropes in male rats",
number = "9",
volume = "32",
pages = "569",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1829"
}

Milošević, V., Sekulić, M. I., Brkić, B., Lovren, M. B.,& Starčević, V. P.. (2000). Effect of centrally administered somatostatin on pituitary thyrotropes in male rats. in Histochemical Journal, 32(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1829

Milošević V, Sekulić MI, Brkić B, Lovren MB, Starčević VP. Effect of centrally administered somatostatin on pituitary thyrotropes in male rats. in Histochemical Journal. 2000;32(9):null-569.
https://hdl.handle.net/21.15107/rcub_ibiss_1829 .

Milošević, Verica, Sekulić, Milka I., Brkić, Branislava, Lovren, Mirjana B., Starčević, Vesna P., "Effect of centrally administered somatostatin on pituitary thyrotropes in male rats" in Histochemical Journal, 32, no. 9 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1829 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1838
AB  - The results obtained during the studies on the effects of gonadal steroid (estradiol dipropionate, EDP) and peptide hormones (luteinizing-releasing hormone, LHRH) on gonadotrophic cell in female rats during different periods of life are summarized. Our results show that gonadotrophic cells are sensitive to a single dose of neonatally administered EDP, so that a serious defect in follicle stimulating hormone (FSH)-, luteinizing hormone (LH)- and prolactin (PRL)-producing cells, and their regulation within the pituitary during infantile, peripubertal and adult period was evident. A reciprocal relationship between the number of gonadotrophic and luteotrophic (PRL) cells during these periods was characteristic. The degree of sensitivity gradually decreased, i.e. it was less conversaly related to the duration of the interval after estrogen treatment. Chronic EDP application to ageing female rats inhibited gonadotrophic cell function, while stimulating hyperplasia and hypertrophy of chromophobes and PRL cells. The suppression of gonadotrophic cell function following LHRH agonist treatment depends on the regimen and dose administration. We showed that maximal suppression of all examined parameters of gonadotrophic cells was achieved by a combined chronic EDP and LHRH treatment.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - The sensitivity of gonadotrophic cells to steroid and peptide hormones
IS  - 1
VL  - 19
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1838
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I.",
year = "2000",
abstract = "The results obtained during the studies on the effects of gonadal steroid (estradiol dipropionate, EDP) and peptide hormones (luteinizing-releasing hormone, LHRH) on gonadotrophic cell in female rats during different periods of life are summarized. Our results show that gonadotrophic cells are sensitive to a single dose of neonatally administered EDP, so that a serious defect in follicle stimulating hormone (FSH)-, luteinizing hormone (LH)- and prolactin (PRL)-producing cells, and their regulation within the pituitary during infantile, peripubertal and adult period was evident. A reciprocal relationship between the number of gonadotrophic and luteotrophic (PRL) cells during these periods was characteristic. The degree of sensitivity gradually decreased, i.e. it was less conversaly related to the duration of the interval after estrogen treatment. Chronic EDP application to ageing female rats inhibited gonadotrophic cell function, while stimulating hyperplasia and hypertrophy of chromophobes and PRL cells. The suppression of gonadotrophic cell function following LHRH agonist treatment depends on the regimen and dose administration. We showed that maximal suppression of all examined parameters of gonadotrophic cells was achieved by a combined chronic EDP and LHRH treatment.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "The sensitivity of gonadotrophic cells to steroid and peptide hormones",
number = "1",
volume = "19",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1838"
}

Lovren, M. B.,& Sekulić, M. I.. (2000). The sensitivity of gonadotrophic cells to steroid and peptide hormones. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 19(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1838

Lovren MB, Sekulić MI. The sensitivity of gonadotrophic cells to steroid and peptide hormones. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 2000;19(1):null-13.
https://hdl.handle.net/21.15107/rcub_ibiss_1838 .

Lovren, Mirjana B., Sekulić, Milka I., "The sensitivity of gonadotrophic cells to steroid and peptide hormones" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 19, no. 1 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1838 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Micić, M
AU  - Radulović, N
PY  - 1999
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1841
AB  - The effects of multiple treatment with estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) or a combination of the two on gonadotrophic cells in the pituitary pars distalis of middle-aged female rats were examined. The animals were treated daily for two weeks with EDP (0.625 mg i.p./kg body weight) or Ca (11.4 mg/kg body weight) or EDP+Ca. Luteinising (LH) and follicle stimulating hormone (FSH)-producing cells were examined by immunohistochemistry using antisera to the specific (beta) beta-subunits of LH and FSH and a peroxidase-anti-peroxidase immunohistochemical procedure. Plasma levels of FSH and LH were measured by radio-immune assay. A stereological method for determining morphometric parameters in immunopositive FSH and LH cells was used. The number of gonadotrophs per unit area (mm(2)), their cellular volume and relative volume densities, as well as plasma levels of FSH and LH, were decreased in all treated females in comparison with the controls. The most significant decrease of these parameters was observed in EDP-treated animals. Such changes were also expressed in Ca-treated animals, but the alterations were less distinct. These results demonstrate that multiple EDP or Ca application to middle-aged female rats is able to inhibit, directly or indirectly, the morphofunctional state of gonadotrophic cells in the pituitary pars distalis.
T2  - Histochemical Journal
T1  - Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats
IS  - 10
VL  - 31
EP  - 676
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1841
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Micić, M and Radulović, N",
year = "1999",
abstract = "The effects of multiple treatment with estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) or a combination of the two on gonadotrophic cells in the pituitary pars distalis of middle-aged female rats were examined. The animals were treated daily for two weeks with EDP (0.625 mg i.p./kg body weight) or Ca (11.4 mg/kg body weight) or EDP+Ca. Luteinising (LH) and follicle stimulating hormone (FSH)-producing cells were examined by immunohistochemistry using antisera to the specific (beta) beta-subunits of LH and FSH and a peroxidase-anti-peroxidase immunohistochemical procedure. Plasma levels of FSH and LH were measured by radio-immune assay. A stereological method for determining morphometric parameters in immunopositive FSH and LH cells was used. The number of gonadotrophs per unit area (mm(2)), their cellular volume and relative volume densities, as well as plasma levels of FSH and LH, were decreased in all treated females in comparison with the controls. The most significant decrease of these parameters was observed in EDP-treated animals. Such changes were also expressed in Ca-treated animals, but the alterations were less distinct. These results demonstrate that multiple EDP or Ca application to middle-aged female rats is able to inhibit, directly or indirectly, the morphofunctional state of gonadotrophic cells in the pituitary pars distalis.",
journal = "Histochemical Journal",
title = "Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats",
number = "10",
volume = "31",
pages = "676",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1841"
}

Lovren, M. B., Sekulić, M. I., Milosević, V., Micić, M.,& Radulović, N.. (1999). Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats. in Histochemical Journal, 31(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1841

Lovren MB, Sekulić MI, Milosević V, Micić M, Radulović N. Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats. in Histochemical Journal. 1999;31(10):null-676.
https://hdl.handle.net/21.15107/rcub_ibiss_1841 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Micić, M, Radulović, N, "Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats" in Histochemical Journal, 31, no. 10 (1999),
https://hdl.handle.net/21.15107/rcub_ibiss_1841 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Velkovski, SD
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
AU  - Starcević, V
AU  - Severs, Walter B
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1859
AB  - This study examined effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 and SRIH-28) on growth and function of pituitary somatotropes (GH cells) and lactotropes (PRL cells), Male rats received three i.c.v. injections (1 mu g/5 mu l) of SRIH-14 or SRIH-28 every second day, Blood samples were collected for hormone assays and pituitaries were removed for histological and morphometric evaluation 5 days after the last i.c.v. treatment, Compared to control animals, SRIH treatment decreased (p < 0.05) pituitary weight and all morphometric measurements obtained in GH and PRL cells, Concentrations of serum growth hormone (GH) and prolactin (PRL) in both SRIH-treated groups were also lower (p < 0.05) than in control rats, These findings suggest that centrally administered somatostatin is specifically involved in the control of growth and secretory activity of GH and PRL cells, Thus, pharmacological manipulation of SRIH receptors reached from cerebrospinal fluid may alter systemic effects of GH and PRL.
T2  - Pharmacology
T1  - Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin
IS  - 1
VL  - 57
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1859
ER  - 

@article{
author = "Milošević, Verica and Brkić, B and Velkovski, SD and Sekulić, Milka I. and Lovren, Mirjana B. and Starcević, V and Severs, Walter B",
year = "1998",
abstract = "This study examined effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 and SRIH-28) on growth and function of pituitary somatotropes (GH cells) and lactotropes (PRL cells), Male rats received three i.c.v. injections (1 mu g/5 mu l) of SRIH-14 or SRIH-28 every second day, Blood samples were collected for hormone assays and pituitaries were removed for histological and morphometric evaluation 5 days after the last i.c.v. treatment, Compared to control animals, SRIH treatment decreased (p < 0.05) pituitary weight and all morphometric measurements obtained in GH and PRL cells, Concentrations of serum growth hormone (GH) and prolactin (PRL) in both SRIH-treated groups were also lower (p < 0.05) than in control rats, These findings suggest that centrally administered somatostatin is specifically involved in the control of growth and secretory activity of GH and PRL cells, Thus, pharmacological manipulation of SRIH receptors reached from cerebrospinal fluid may alter systemic effects of GH and PRL.",
journal = "Pharmacology",
title = "Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin",
number = "1",
volume = "57",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1859"
}

Milošević, V., Brkić, B., Velkovski, S., Sekulić, M. I., Lovren, M. B., Starcević, V.,& Severs, W. B.. (1998). Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin. in Pharmacology, 57(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1859

Milošević V, Brkić B, Velkovski S, Sekulić MI, Lovren MB, Starcević V, Severs WB. Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin. in Pharmacology. 1998;57(1):null-34.
https://hdl.handle.net/21.15107/rcub_ibiss_1859 .

Milošević, Verica, Brkić, B, Velkovski, SD, Sekulić, Milka I., Lovren, Mirjana B., Starcević, V, Severs, Walter B, "Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin" in Pharmacology, 57, no. 1 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1859 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
AU  - Milosević, V
AU  - Micić, M
AU  - Balint-Perić, L
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1862
AB  - The structure and function of C cells of middle-aged female rats (14-months old) treated with estradiol dipropionate (EDP), calcium (Ca) or a combination of EDP+Ca were studied. A stereological method was used to determine the volume of calcitonin (CT)-immunoreactive C cells and their nuclei, and the relative volume density and mean number of the C cells per section were calculated. Serum levels of CT, osteocalcin, parathyroid hormone (PTH), and beta-estradiol were also measured. A significant decrease in body weight of the rats treated with EDP or EDP+Ca was observed. These treatments led to a significant decrease in cellular and nuclear volumes, relative volume density, and mean number of C cells per section, in comparison with the corresponding controls. A reduction of the serum level of CT, PTH, and osteocalcin was also recorded in EDP-and EDP+Ca-treated animals. No statistically significant differences between Ca- and vehicle-injected rats, with regard to all morphometric C cell parameters and biochemical values determined, were seen. However, a conspicuous degranulation of the C cells and decreased immunoreactivity for CT in the Ca-receiving group, which could be interpreted as the signs of increased activity of these cells, were noticed. This effect of Ca was also observed in rats injected with EDP and Ca in combination, when the inhibitory effect of EDP on C cell function was less noticeable than in the group treated with EDP alone.
T2  - Histochemistry and Cell Biology
T1  - Thyroid C cells of middle-aged rats treated with estradiol or calcium
IS  - 3
VL  - 109
EP  - 262
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1862
ER  - 

@article{
author = "Sekulić, Milka I. and Lovren, Mirjana B. and Milosević, V and Micić, M and Balint-Perić, L",
year = "1998",
abstract = "The structure and function of C cells of middle-aged female rats (14-months old) treated with estradiol dipropionate (EDP), calcium (Ca) or a combination of EDP+Ca were studied. A stereological method was used to determine the volume of calcitonin (CT)-immunoreactive C cells and their nuclei, and the relative volume density and mean number of the C cells per section were calculated. Serum levels of CT, osteocalcin, parathyroid hormone (PTH), and beta-estradiol were also measured. A significant decrease in body weight of the rats treated with EDP or EDP+Ca was observed. These treatments led to a significant decrease in cellular and nuclear volumes, relative volume density, and mean number of C cells per section, in comparison with the corresponding controls. A reduction of the serum level of CT, PTH, and osteocalcin was also recorded in EDP-and EDP+Ca-treated animals. No statistically significant differences between Ca- and vehicle-injected rats, with regard to all morphometric C cell parameters and biochemical values determined, were seen. However, a conspicuous degranulation of the C cells and decreased immunoreactivity for CT in the Ca-receiving group, which could be interpreted as the signs of increased activity of these cells, were noticed. This effect of Ca was also observed in rats injected with EDP and Ca in combination, when the inhibitory effect of EDP on C cell function was less noticeable than in the group treated with EDP alone.",
journal = "Histochemistry and Cell Biology",
title = "Thyroid C cells of middle-aged rats treated with estradiol or calcium",
number = "3",
volume = "109",
pages = "262",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1862"
}

Sekulić, M. I., Lovren, M. B., Milosević, V., Micić, M.,& Balint-Perić, L.. (1998). Thyroid C cells of middle-aged rats treated with estradiol or calcium. in Histochemistry and Cell Biology, 109(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1862

Sekulić MI, Lovren MB, Milosević V, Micić M, Balint-Perić L. Thyroid C cells of middle-aged rats treated with estradiol or calcium. in Histochemistry and Cell Biology. 1998;109(3):null-262.
https://hdl.handle.net/21.15107/rcub_ibiss_1862 .

Sekulić, Milka I., Lovren, Mirjana B., Milosević, V, Micić, M, Balint-Perić, L, "Thyroid C cells of middle-aged rats treated with estradiol or calcium" in Histochemistry and Cell Biology, 109, no. 3 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1862 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
AU  - Milosević, V
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1861
AB  - The pituitary TSH cell structure of middle-aged (14-month-old) female Wistar rats chronically treated with estradiol dipropionate (EDP), calcium glucoheptonate (Ca) or with the combination of both was studied. TSH-producing cells were examined in the pituitary pars distalis using rabbit anti-rat beta-thyrotropin (TSH) serum and peroxidase-antiperoxidase immunohistochemistry. A stereological method for the determination of morphometric changes of the volume of TSH cells and nuclei as well as of their number and relative volume densities was used. All examined morphometric parameters in treated animals showed a significant decrease in comparison with immunoreactive TSH cells of the controls; the most significant decrease was observed in EDP-treated rats. These results together with structural features of immunoreactive TSH cells in the pituitary of middle-aged rats after chronic application of EDP or Ca indicate that both compounds inhibit these cells.
T2  - Acta Histochemica
T1  - Immunoreactive TSH cells in the pituitary of female middle-aged rats after treatment with estradiol or calcium
IS  - 2
VL  - 100
EP  - 191
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1861
ER  - 

@article{
author = "Sekulić, Milka I. and Lovren, Mirjana B. and Milosević, V",
year = "1998",
abstract = "The pituitary TSH cell structure of middle-aged (14-month-old) female Wistar rats chronically treated with estradiol dipropionate (EDP), calcium glucoheptonate (Ca) or with the combination of both was studied. TSH-producing cells were examined in the pituitary pars distalis using rabbit anti-rat beta-thyrotropin (TSH) serum and peroxidase-antiperoxidase immunohistochemistry. A stereological method for the determination of morphometric changes of the volume of TSH cells and nuclei as well as of their number and relative volume densities was used. All examined morphometric parameters in treated animals showed a significant decrease in comparison with immunoreactive TSH cells of the controls; the most significant decrease was observed in EDP-treated rats. These results together with structural features of immunoreactive TSH cells in the pituitary of middle-aged rats after chronic application of EDP or Ca indicate that both compounds inhibit these cells.",
journal = "Acta Histochemica",
title = "Immunoreactive TSH cells in the pituitary of female middle-aged rats after treatment with estradiol or calcium",
number = "2",
volume = "100",
pages = "191",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1861"
}

Sekulić, M. I., Lovren, M. B.,& Milosević, V.. (1998). Immunoreactive TSH cells in the pituitary of female middle-aged rats after treatment with estradiol or calcium. in Acta Histochemica, 100(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1861

Sekulić MI, Lovren MB, Milosević V. Immunoreactive TSH cells in the pituitary of female middle-aged rats after treatment with estradiol or calcium. in Acta Histochemica. 1998;100(2):null-191.
https://hdl.handle.net/21.15107/rcub_ibiss_1861 .

Sekulić, Milka I., Lovren, Mirjana B., Milosević, V, "Immunoreactive TSH cells in the pituitary of female middle-aged rats after treatment with estradiol or calcium" in Acta Histochemica, 100, no. 2 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1861 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Radulović, N
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1856
AB  - The effects of intracerebroventricular application of SRIF-14 and SRIF-28 on pituitary gonadotrophic cells (FSH and LH) were examined using immunocytochemical and morphometrical methods in adult female Wistar rats. FSH- and LH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were used to evaluate changes in the number, volume densities and relative volume densities of LH- and FSH-immunopositive cells. In females treated with SRIF-14 or SRIF-28, the gonadotrophs were smaller, often pycnotic and more intensely stained. The number of LH-positive cells per unit area (mm(2)) was significantly decreased in both somatostatin-treated groups, while the number of FSH-positive cells was similar to that in the controls. Volume densities of perykarya of LH- and FSH-positive cells were decreased in all treated females, but extremely different in LH-positive cells after SRIF-14 administration. The relative volume density of LH cells was significantly decreased in both somatostatin-treated groups, while immunopositive FSH cells were not significantly decreased compared with the controls. It can be concluded that centrally applied somatostatins lead to changes in the immunocytochemical and morphometric properties of LH cells, while there is no significant effect on FSH cells.
T2  - Acta Histochemica
T1  - Effects of somatostatins on gonadotrophic cells in female rats
IS  - 3
VL  - 100
EP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1856
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Radulović, N",
year = "1998",
abstract = "The effects of intracerebroventricular application of SRIF-14 and SRIF-28 on pituitary gonadotrophic cells (FSH and LH) were examined using immunocytochemical and morphometrical methods in adult female Wistar rats. FSH- and LH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were used to evaluate changes in the number, volume densities and relative volume densities of LH- and FSH-immunopositive cells. In females treated with SRIF-14 or SRIF-28, the gonadotrophs were smaller, often pycnotic and more intensely stained. The number of LH-positive cells per unit area (mm(2)) was significantly decreased in both somatostatin-treated groups, while the number of FSH-positive cells was similar to that in the controls. Volume densities of perykarya of LH- and FSH-positive cells were decreased in all treated females, but extremely different in LH-positive cells after SRIF-14 administration. The relative volume density of LH cells was significantly decreased in both somatostatin-treated groups, while immunopositive FSH cells were not significantly decreased compared with the controls. It can be concluded that centrally applied somatostatins lead to changes in the immunocytochemical and morphometric properties of LH cells, while there is no significant effect on FSH cells.",
journal = "Acta Histochemica",
title = "Effects of somatostatins on gonadotrophic cells in female rats",
number = "3",
volume = "100",
pages = "335",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1856"
}

Lovren, M. B., Sekulić, M. I., Milosević, V.,& Radulović, N.. (1998). Effects of somatostatins on gonadotrophic cells in female rats. in Acta Histochemica, 100(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1856

Lovren MB, Sekulić MI, Milosević V, Radulović N. Effects of somatostatins on gonadotrophic cells in female rats. in Acta Histochemica. 1998;100(3):null-335.
https://hdl.handle.net/21.15107/rcub_ibiss_1856 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Radulović, N, "Effects of somatostatins on gonadotrophic cells in female rats" in Acta Histochemica, 100, no. 3 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1856 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Velkovski, S
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Starcević, V
PY  - 1997
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1878
AB  - The effects of the intracerebroventricular (i.c.v.) administration of somatostatin (SRIF-14) on zona glomerulosa (ZG), zona fasciculata (ZF) and zona reticularis (ZR) of the adrenal cortex and an the blood concentrations of adrenocorticotropic hormone (ACTH), prolactin (PRL), growth hormone (GH), aldosterone, cortisol, progesterone and estradiol in male rats were studied. Each experimental animal was given three 1 mu g doses of SRIF-14 dissolved in 5 mu L saline by i.c.v. route every second day. Controls were treated in the same way with the same volume of saline. The rats were sacrificed by decapitation five days after the last SRIF-14 dose. The left adrenal gland of each animal was dissected and prepared for histological and morphometric analyses. Blood samples were collected for hormone level determinations. SRIF-14 led to a significant reduction (p < 0.05) of the absolute volume of the adrenal gland and of the volume of the cortex and ZG in comparison with the controls. Total number and volume of ZG cells and their nuclei was also significantly reduced (p < 0.05) in SRIF-14-treated rats as compared to the controls. There were no significant changes in morphometric parameters of ZF and ZR. At the same time, a significant decrease (p < 0.05) of aldosterone, GH, and PRL levels were observed, while the concentration of ACTH, cortisol, progesterone, and estradiol were almost unaltered in comparison with control values. It can be concluded that centrally applied SRIF-14 inhibits both the secretory activity and growth of adrenal ZG cells only, while insignificantly affecting ZF and ZR cells.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - The effects of centrally applied somatostatin on the adrenal cortex of male rats
IS  - 2
VL  - 16
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1878
ER  - 

@article{
author = "Milošević, Verica and Brkić, B and Velkovski, S and Lovren, Mirjana B. and Sekulić, Milka I. and Starcević, V",
year = "1997",
abstract = "The effects of the intracerebroventricular (i.c.v.) administration of somatostatin (SRIF-14) on zona glomerulosa (ZG), zona fasciculata (ZF) and zona reticularis (ZR) of the adrenal cortex and an the blood concentrations of adrenocorticotropic hormone (ACTH), prolactin (PRL), growth hormone (GH), aldosterone, cortisol, progesterone and estradiol in male rats were studied. Each experimental animal was given three 1 mu g doses of SRIF-14 dissolved in 5 mu L saline by i.c.v. route every second day. Controls were treated in the same way with the same volume of saline. The rats were sacrificed by decapitation five days after the last SRIF-14 dose. The left adrenal gland of each animal was dissected and prepared for histological and morphometric analyses. Blood samples were collected for hormone level determinations. SRIF-14 led to a significant reduction (p < 0.05) of the absolute volume of the adrenal gland and of the volume of the cortex and ZG in comparison with the controls. Total number and volume of ZG cells and their nuclei was also significantly reduced (p < 0.05) in SRIF-14-treated rats as compared to the controls. There were no significant changes in morphometric parameters of ZF and ZR. At the same time, a significant decrease (p < 0.05) of aldosterone, GH, and PRL levels were observed, while the concentration of ACTH, cortisol, progesterone, and estradiol were almost unaltered in comparison with control values. It can be concluded that centrally applied SRIF-14 inhibits both the secretory activity and growth of adrenal ZG cells only, while insignificantly affecting ZF and ZR cells.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "The effects of centrally applied somatostatin on the adrenal cortex of male rats",
number = "2",
volume = "16",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1878"
}

Milošević, V., Brkić, B., Velkovski, S., Lovren, M. B., Sekulić, M. I.,& Starcević, V.. (1997). The effects of centrally applied somatostatin on the adrenal cortex of male rats. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1878

Milošević V, Brkić B, Velkovski S, Lovren MB, Sekulić MI, Starcević V. The effects of centrally applied somatostatin on the adrenal cortex of male rats. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 1997;16(2):null-94.
https://hdl.handle.net/21.15107/rcub_ibiss_1878 .

Milošević, Verica, Brkić, B, Velkovski, S, Lovren, Mirjana B., Sekulić, Milka I., Starcević, V, "The effects of centrally applied somatostatin on the adrenal cortex of male rats" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16, no. 2 (1997),
https://hdl.handle.net/21.15107/rcub_ibiss_1878 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Micić, M
AU  - Radulović, N
PY  - 1997
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1877
AB  - Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment
IS  - 2
VL  - 16
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1877
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Micić, M and Radulović, N",
year = "1997",
abstract = "Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment",
number = "2",
volume = "16",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1877"
}

Lovren, M. B., Sekulić, M. I., Milosević, V., Micić, M.,& Radulović, N.. (1997). Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1877

Lovren MB, Sekulić MI, Milosević V, Micić M, Radulović N. Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 1997;16(2):null-87.
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Micić, M, Radulović, N, "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16, no. 2 (1997),
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
AU  - Milosević, V
PY  - 1996
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1891
AB  - The subject of these studies was the thyroid gland of the male aged rats (24-month-old), treated with sex steroids. Estradiol-dipropionate (EDP) in a dose of 0.5 mg/animal and testosterone-propionate (TP) in a dose of 3 mg/animal were s.c. injected to male rats every second day during 30 days. The serial sections of whole thyroid gland were used for stereological analyses and determination of the following morphometric parameters: the height and volumetric density of follicular epithelium, volumetric density of colloid and interstitium, and index of activation rate. The height of follicular epithelium, its volume density, and index of activation rate were significantly reduced in all hormone-treated animals. However, the volumetric density of colloid was significantly higher in EDP- and TP-treated animals (108% and 61%, respectively) in comparison with the corresponding controls. These morphometric parameters and cytologic features of thyroid gland of aged rats after chronic treatment with sex steroids indicate that these hormones express an inhibitory effect on thyroid follicular cells, but these changes were more prominent in animals treated with EDP than in those receiving TP.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Estradiol- and testosterone-related changes of the thyroid gland structure in aged rats
IS  - 2
VL  - 15
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1891
ER  - 

@article{
author = "Sekulić, Milka I. and Lovren, Mirjana B. and Milosević, V",
year = "1996",
abstract = "The subject of these studies was the thyroid gland of the male aged rats (24-month-old), treated with sex steroids. Estradiol-dipropionate (EDP) in a dose of 0.5 mg/animal and testosterone-propionate (TP) in a dose of 3 mg/animal were s.c. injected to male rats every second day during 30 days. The serial sections of whole thyroid gland were used for stereological analyses and determination of the following morphometric parameters: the height and volumetric density of follicular epithelium, volumetric density of colloid and interstitium, and index of activation rate. The height of follicular epithelium, its volume density, and index of activation rate were significantly reduced in all hormone-treated animals. However, the volumetric density of colloid was significantly higher in EDP- and TP-treated animals (108% and 61%, respectively) in comparison with the corresponding controls. These morphometric parameters and cytologic features of thyroid gland of aged rats after chronic treatment with sex steroids indicate that these hormones express an inhibitory effect on thyroid follicular cells, but these changes were more prominent in animals treated with EDP than in those receiving TP.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Estradiol- and testosterone-related changes of the thyroid gland structure in aged rats",
number = "2",
volume = "15",
pages = "92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1891"
}

Sekulić, M. I., Lovren, M. B.,& Milosević, V.. (1996). Estradiol- and testosterone-related changes of the thyroid gland structure in aged rats. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 15(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1891

Sekulić MI, Lovren MB, Milosević V. Estradiol- and testosterone-related changes of the thyroid gland structure in aged rats. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 1996;15(2):null-92.
https://hdl.handle.net/21.15107/rcub_ibiss_1891 .

Sekulić, Milka I., Lovren, Mirjana B., Milosević, V, "Estradiol- and testosterone-related changes of the thyroid gland structure in aged rats" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 15, no. 2 (1996),
https://hdl.handle.net/21.15107/rcub_ibiss_1891 .