TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Micić, M
AU  - Radulović, N
PY  - 1999
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1841
AB  - The effects of multiple treatment with estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) or a combination of the two on gonadotrophic cells in the pituitary pars distalis of middle-aged female rats were examined. The animals were treated daily for two weeks with EDP (0.625 mg i.p./kg body weight) or Ca (11.4 mg/kg body weight) or EDP+Ca. Luteinising (LH) and follicle stimulating hormone (FSH)-producing cells were examined by immunohistochemistry using antisera to the specific (beta) beta-subunits of LH and FSH and a peroxidase-anti-peroxidase immunohistochemical procedure. Plasma levels of FSH and LH were measured by radio-immune assay. A stereological method for determining morphometric parameters in immunopositive FSH and LH cells was used. The number of gonadotrophs per unit area (mm(2)), their cellular volume and relative volume densities, as well as plasma levels of FSH and LH, were decreased in all treated females in comparison with the controls. The most significant decrease of these parameters was observed in EDP-treated animals. Such changes were also expressed in Ca-treated animals, but the alterations were less distinct. These results demonstrate that multiple EDP or Ca application to middle-aged female rats is able to inhibit, directly or indirectly, the morphofunctional state of gonadotrophic cells in the pituitary pars distalis.
T2  - Histochemical Journal
T1  - Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats
IS  - 10
VL  - 31
EP  - 676
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1841
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Micić, M and Radulović, N",
year = "1999",
abstract = "The effects of multiple treatment with estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) or a combination of the two on gonadotrophic cells in the pituitary pars distalis of middle-aged female rats were examined. The animals were treated daily for two weeks with EDP (0.625 mg i.p./kg body weight) or Ca (11.4 mg/kg body weight) or EDP+Ca. Luteinising (LH) and follicle stimulating hormone (FSH)-producing cells were examined by immunohistochemistry using antisera to the specific (beta) beta-subunits of LH and FSH and a peroxidase-anti-peroxidase immunohistochemical procedure. Plasma levels of FSH and LH were measured by radio-immune assay. A stereological method for determining morphometric parameters in immunopositive FSH and LH cells was used. The number of gonadotrophs per unit area (mm(2)), their cellular volume and relative volume densities, as well as plasma levels of FSH and LH, were decreased in all treated females in comparison with the controls. The most significant decrease of these parameters was observed in EDP-treated animals. Such changes were also expressed in Ca-treated animals, but the alterations were less distinct. These results demonstrate that multiple EDP or Ca application to middle-aged female rats is able to inhibit, directly or indirectly, the morphofunctional state of gonadotrophic cells in the pituitary pars distalis.",
journal = "Histochemical Journal",
title = "Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats",
number = "10",
volume = "31",
pages = "676",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1841"
}

Lovren, M. B., Sekulić, M. I., Milosević, V., Micić, M.,& Radulović, N.. (1999). Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats. in Histochemical Journal, 31(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1841

Lovren MB, Sekulić MI, Milosević V, Micić M, Radulović N. Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats. in Histochemical Journal. 1999;31(10):null-676.
https://hdl.handle.net/21.15107/rcub_ibiss_1841 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Micić, M, Radulović, N, "Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats" in Histochemical Journal, 31, no. 10 (1999),
https://hdl.handle.net/21.15107/rcub_ibiss_1841 .

TY  - CONF
AU  - Dergović, D
AU  - Micić, M
AU  - Zivković, I
AU  - Koezević, J
AU  - Milosević, V
AU  - Starcević, V
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1864
C3  - European Journal of Neuroscience
T1  - The thymic monoaminergic innervation after centrally applied somatostatin in young rats
IS  - null
VL  - 10
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1864
ER  - 

@conference{
author = "Dergović, D and Micić, M and Zivković, I and Koezević, J and Milosević, V and Starcević, V",
year = "1998",
journal = "European Journal of Neuroscience",
title = "The thymic monoaminergic innervation after centrally applied somatostatin in young rats",
number = "null",
volume = "10",
pages = "232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1864"
}

Dergović, D., Micić, M., Zivković, I., Koezević, J., Milosević, V.,& Starcević, V.. (1998). The thymic monoaminergic innervation after centrally applied somatostatin in young rats. in European Journal of Neuroscience, 10(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1864

Dergović D, Micić M, Zivković I, Koezević J, Milosević V, Starcević V. The thymic monoaminergic innervation after centrally applied somatostatin in young rats. in European Journal of Neuroscience. 1998;10(null):null-232.
https://hdl.handle.net/21.15107/rcub_ibiss_1864 .

Dergović, D, Micić, M, Zivković, I, Koezević, J, Milosević, V, Starcević, V, "The thymic monoaminergic innervation after centrally applied somatostatin in young rats" in European Journal of Neuroscience, 10, no. null (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1864 .

TY  - CONF
AU  - Micić, M
AU  - Dergović, D
AU  - Zivković, I
AU  - Knežević, J
AU  - Milosević, V
AU  - Starcević, V
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1863
C3  - European Journal of Neuroscience
T1  - The thymic cholinergic innervation of young rats centrally treated with somatostatin
IS  - null
VL  - 10
EP  - 229
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1863
ER  - 

@conference{
author = "Micić, M and Dergović, D and Zivković, I and Knežević, J and Milosević, V and Starcević, V",
year = "1998",
journal = "European Journal of Neuroscience",
title = "The thymic cholinergic innervation of young rats centrally treated with somatostatin",
number = "null",
volume = "10",
pages = "229",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1863"
}

Micić, M., Dergović, D., Zivković, I., Knežević, J., Milosević, V.,& Starcević, V.. (1998). The thymic cholinergic innervation of young rats centrally treated with somatostatin. in European Journal of Neuroscience, 10(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1863

Micić M, Dergović D, Zivković I, Knežević J, Milosević V, Starcević V. The thymic cholinergic innervation of young rats centrally treated with somatostatin. in European Journal of Neuroscience. 1998;10(null):null-229.
https://hdl.handle.net/21.15107/rcub_ibiss_1863 .

Micić, M, Dergović, D, Zivković, I, Knežević, J, Milosević, V, Starcević, V, "The thymic cholinergic innervation of young rats centrally treated with somatostatin" in European Journal of Neuroscience, 10, no. null (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1863 .

TY  - JOUR
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
AU  - Milosević, V
AU  - Micić, M
AU  - Balint-Perić, L
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1862
AB  - The structure and function of C cells of middle-aged female rats (14-months old) treated with estradiol dipropionate (EDP), calcium (Ca) or a combination of EDP+Ca were studied. A stereological method was used to determine the volume of calcitonin (CT)-immunoreactive C cells and their nuclei, and the relative volume density and mean number of the C cells per section were calculated. Serum levels of CT, osteocalcin, parathyroid hormone (PTH), and beta-estradiol were also measured. A significant decrease in body weight of the rats treated with EDP or EDP+Ca was observed. These treatments led to a significant decrease in cellular and nuclear volumes, relative volume density, and mean number of C cells per section, in comparison with the corresponding controls. A reduction of the serum level of CT, PTH, and osteocalcin was also recorded in EDP-and EDP+Ca-treated animals. No statistically significant differences between Ca- and vehicle-injected rats, with regard to all morphometric C cell parameters and biochemical values determined, were seen. However, a conspicuous degranulation of the C cells and decreased immunoreactivity for CT in the Ca-receiving group, which could be interpreted as the signs of increased activity of these cells, were noticed. This effect of Ca was also observed in rats injected with EDP and Ca in combination, when the inhibitory effect of EDP on C cell function was less noticeable than in the group treated with EDP alone.
T2  - Histochemistry and Cell Biology
T1  - Thyroid C cells of middle-aged rats treated with estradiol or calcium
IS  - 3
VL  - 109
EP  - 262
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1862
ER  - 

@article{
author = "Sekulić, Milka I. and Lovren, Mirjana B. and Milosević, V and Micić, M and Balint-Perić, L",
year = "1998",
abstract = "The structure and function of C cells of middle-aged female rats (14-months old) treated with estradiol dipropionate (EDP), calcium (Ca) or a combination of EDP+Ca were studied. A stereological method was used to determine the volume of calcitonin (CT)-immunoreactive C cells and their nuclei, and the relative volume density and mean number of the C cells per section were calculated. Serum levels of CT, osteocalcin, parathyroid hormone (PTH), and beta-estradiol were also measured. A significant decrease in body weight of the rats treated with EDP or EDP+Ca was observed. These treatments led to a significant decrease in cellular and nuclear volumes, relative volume density, and mean number of C cells per section, in comparison with the corresponding controls. A reduction of the serum level of CT, PTH, and osteocalcin was also recorded in EDP-and EDP+Ca-treated animals. No statistically significant differences between Ca- and vehicle-injected rats, with regard to all morphometric C cell parameters and biochemical values determined, were seen. However, a conspicuous degranulation of the C cells and decreased immunoreactivity for CT in the Ca-receiving group, which could be interpreted as the signs of increased activity of these cells, were noticed. This effect of Ca was also observed in rats injected with EDP and Ca in combination, when the inhibitory effect of EDP on C cell function was less noticeable than in the group treated with EDP alone.",
journal = "Histochemistry and Cell Biology",
title = "Thyroid C cells of middle-aged rats treated with estradiol or calcium",
number = "3",
volume = "109",
pages = "262",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1862"
}

Sekulić, M. I., Lovren, M. B., Milosević, V., Micić, M.,& Balint-Perić, L.. (1998). Thyroid C cells of middle-aged rats treated with estradiol or calcium. in Histochemistry and Cell Biology, 109(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1862

Sekulić MI, Lovren MB, Milosević V, Micić M, Balint-Perić L. Thyroid C cells of middle-aged rats treated with estradiol or calcium. in Histochemistry and Cell Biology. 1998;109(3):null-262.
https://hdl.handle.net/21.15107/rcub_ibiss_1862 .

Sekulić, Milka I., Lovren, Mirjana B., Milosević, V, Micić, M, Balint-Perić, L, "Thyroid C cells of middle-aged rats treated with estradiol or calcium" in Histochemistry and Cell Biology, 109, no. 3 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1862 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Micić, M
AU  - Radulović, N
PY  - 1997
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1877
AB  - Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment
IS  - 2
VL  - 16
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1877
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Micić, M and Radulović, N",
year = "1997",
abstract = "Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment",
number = "2",
volume = "16",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1877"
}

Lovren, M. B., Sekulić, M. I., Milosević, V., Micić, M.,& Radulović, N.. (1997). Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1877

Lovren MB, Sekulić MI, Milosević V, Micić M, Radulović N. Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 1997;16(2):null-87.
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Micić, M, Radulović, N, "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16, no. 2 (1997),
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .