@article{
author = "Lazic, K. and Petrovic, J. and Ciric, J. and Kalauzi, A. and Šaponjić, Jasna",
year = "2015",
abstract = "Objectives: We hypothesized that the impact of distinct anesthetic
regimens could be differently expressed during anesthesia and on
post-anesthesia sleep in the neurodegenerative diseases. Therefore, we
followed the impact of ketamine/diazepam and pentobarbital anesthesia in
a rat model of the severe Parkinson's disease cholinergic neuropathology
on the electroencephalographic (EEG) microstructure and respiratory
pattern during anesthesia, and on the post-anesthesia sleep. Methods: We
performed the experiments on adult, male, spontaneously breathing Wistar
rats chronically instrumented for sleep recording. The bilateral
pedunculopontine tegmental nucleus (PPT) lesion was done by ibotenic
acid microinfusion. Following postoperative recovery, we recorded sleep
for 6 h, induced anesthesia 24 h later using ketamine/diazepam or
pentobarbital, and repeated sleep recordings sessions 48 h and 6 days
later. During 20 min of each anesthesia we recorded both the EEG and
respiratory movements. For sleep and EEG analysis, Fourier analysis was
applied on 6-h recordings, and each 10-s epoch was differentiated as a
state of wakefulness (Wake), non-rapid eye movement (NREM) or rapid eye
movement (REM). Additionally, the group probability density
distributions of all EEG frequency band relative amplitudes were
calculated for each state, with particular attention during anesthesia.
For respiratory pattern analysis we used Monotone Signal Segments
Analysis. The PPT lesion was identified through nicotinamide adenine
dinucleotide phosphate (NADPH) diaphorase histochemistry. Results and
conclusions: Our data show that the ketamine/diazepam anesthetic regimen
in the PPT-lesioned rats induces more alterations in the EEG
microstructure and respiratory pattern than does the pentobarbital
anesthesia. In addition, the equal time required to establish an
anesthetized state, and the long-term effect on postanesthesia sleep in
the PPT-lesioned vs. control rats suggest this anesthetic regimen as
potentially more beneficial both for anesthesia induction and for
post-anesthesia sleep in the surgical procedures of the elderly, and
Parkinson's, and Alzheimer's patients. (C) 2015 IBRO. Published by
Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Impact of anesthetic regimen on the respiratory pattern, EEG microstructure and sleep in the rat model of cholinergic Parkinson's disease neuropathology",
volume = "304",
doi = "10.1016/j.neuroscience.2015.07.020",
pages = "1-13"
}