TY  - JOUR
AU  - Schachner-Gröhs, Iris
AU  - Koller, Michael
AU  - Leopold, Melanie
AU  - Kolm, Claudia
AU  - Linke, Rita
AU  - Jakwerth, Stefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kandler, Wolfgang
AU  - Sulyok, Michael
AU  - Vierheilig, Julia
AU  - Toumi, Marwene
AU  - Farkas, Rózsa
AU  - Toth, Erika
AU  - Kittinger, Clemens
AU  - Zarfel, Gernot
AU  - Farnleitner, Andreas
AU  - Kirschner, Alexander
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6581
AB  - The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are of particular concern as they are highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding of occurrence, spread and key drivers of AMR along whole river courses is largely lacking. We provide a holistic approach by studying spatiotemporal patterns and hotspots of antibiotic resistance genes (ARGs) along 2311 km of the navigable Danube River, combining a longitudinal and temporal monitoring campaign. The integration of advanced faecal pollution diagnostics and environmental and chemical key parameters allowed linking ARG concentrations to the major pollution sources and explaining the observed patterns. Nine AMR markers, including genes conferring resistance to five different antibiotic classes of clinical and environmental relevance, and one integrase gene were determined by probe-based qPCR. All AMR targets could be quantified in Danube River water, with intI1 and sul1 being ubiquitously abundant, qnrS, tetM, blaTEM with intermediate abundance and blaOXA-48like, blaCTX−M-1 group, blaCTX−M-9 group and blaKPC genes with rare occurrence. Human faecal pollution from municipal wastewater discharges was the dominant factor shaping ARG patterns along the Danube River. Other significant correlations of specific ARGs were observed with discharge, certain metals and pesticides. In contrast, intI1 was not associated with wastewater but was already established in the water microbiome. Animal contamination was detected only sporadically and was correlated with ARGs only in the temporal sampling set. During temporal monitoring, an extraordinary hotspot was identified emphasizing the variability within natural waters. This study provides the first comprehensive baseline concentrations of ARGs in the Danube River and lays the foundation for monitoring future trends and evaluating potential reduction measures. The applided holistic approach proved to be a valuable methodological contribution towards a better understanding of the environmental occurrence of AMR.
PB  - Elsevier
T2  - Water Research
T1  - Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River
VL  - 252
DO  - 10.1016/j.watres.2024.121244
SP  - 121244
ER  - 

@article{
author = "Schachner-Gröhs, Iris and Koller, Michael and Leopold, Melanie and Kolm, Claudia and Linke, Rita and Jakwerth, Stefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kandler, Wolfgang and Sulyok, Michael and Vierheilig, Julia and Toumi, Marwene and Farkas, Rózsa and Toth, Erika and Kittinger, Clemens and Zarfel, Gernot and Farnleitner, Andreas and Kirschner, Alexander",
year = "2024",
abstract = "The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are of particular concern as they are highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding of occurrence, spread and key drivers of AMR along whole river courses is largely lacking. We provide a holistic approach by studying spatiotemporal patterns and hotspots of antibiotic resistance genes (ARGs) along 2311 km of the navigable Danube River, combining a longitudinal and temporal monitoring campaign. The integration of advanced faecal pollution diagnostics and environmental and chemical key parameters allowed linking ARG concentrations to the major pollution sources and explaining the observed patterns. Nine AMR markers, including genes conferring resistance to five different antibiotic classes of clinical and environmental relevance, and one integrase gene were determined by probe-based qPCR. All AMR targets could be quantified in Danube River water, with intI1 and sul1 being ubiquitously abundant, qnrS, tetM, blaTEM with intermediate abundance and blaOXA-48like, blaCTX−M-1 group, blaCTX−M-9 group and blaKPC genes with rare occurrence. Human faecal pollution from municipal wastewater discharges was the dominant factor shaping ARG patterns along the Danube River. Other significant correlations of specific ARGs were observed with discharge, certain metals and pesticides. In contrast, intI1 was not associated with wastewater but was already established in the water microbiome. Animal contamination was detected only sporadically and was correlated with ARGs only in the temporal sampling set. During temporal monitoring, an extraordinary hotspot was identified emphasizing the variability within natural waters. This study provides the first comprehensive baseline concentrations of ARGs in the Danube River and lays the foundation for monitoring future trends and evaluating potential reduction measures. The applided holistic approach proved to be a valuable methodological contribution towards a better understanding of the environmental occurrence of AMR.",
publisher = "Elsevier",
journal = "Water Research",
title = "Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River",
volume = "252",
doi = "10.1016/j.watres.2024.121244",
pages = "121244"
}

Schachner-Gröhs, I., Koller, M., Leopold, M., Kolm, C., Linke, R., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Kandler, W., Sulyok, M., Vierheilig, J., Toumi, M., Farkas, R., Toth, E., Kittinger, C., Zarfel, G., Farnleitner, A.,& Kirschner, A.. (2024). Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River. in Water Research
Elsevier., 252, 121244.
https://doi.org/10.1016/j.watres.2024.121244

Schachner-Gröhs I, Koller M, Leopold M, Kolm C, Linke R, Jakwerth S, Kolarević S, Kračun-Kolarević M, Kandler W, Sulyok M, Vierheilig J, Toumi M, Farkas R, Toth E, Kittinger C, Zarfel G, Farnleitner A, Kirschner A. Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River. in Water Research. 2024;252:121244.
doi:10.1016/j.watres.2024.121244 .

Schachner-Gröhs, Iris, Koller, Michael, Leopold, Melanie, Kolm, Claudia, Linke, Rita, Jakwerth, Stefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kandler, Wolfgang, Sulyok, Michael, Vierheilig, Julia, Toumi, Marwene, Farkas, Rózsa, Toth, Erika, Kittinger, Clemens, Zarfel, Gernot, Farnleitner, Andreas, Kirschner, Alexander, "Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River" in Water Research, 252 (2024):121244,
https://doi.org/10.1016/j.watres.2024.121244 . .