TY  - CONF
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Oreščanin Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Stević, Zorica D
AU  - Rakocević-Stojanović, Vidosava M
AU  - Blagojević, Duško
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/988
C3  - Febs Journal
T1  - Erythrocyte SOD1 activity-role in bioavailability of NO and possible cause of 'duing back' phenomena in neurodegenerative diseases
IS  - null
VL  - 280
SP  - 114
EP  - 431
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_988
ER  - 

@conference{
author = "Nikolić-Kokić, Aleksandra and Slavić, Marija and Oreščanin Dušić, Zorana and Spasić, Mihajlo and Stević, Zorica D and Rakocević-Stojanović, Vidosava M and Blagojević, Duško",
year = "2013",
journal = "Febs Journal",
title = "Erythrocyte SOD1 activity-role in bioavailability of NO and possible cause of 'duing back' phenomena in neurodegenerative diseases",
number = "null",
volume = "280",
pages = "114-431",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_988"
}

Nikolić-Kokić, A., Slavić, M., Oreščanin Dušić, Z., Spasić, M., Stević, Z. D., Rakocević-Stojanović, V. M.,& Blagojević, D.. (2013). Erythrocyte SOD1 activity-role in bioavailability of NO and possible cause of 'duing back' phenomena in neurodegenerative diseases. in Febs Journal, 280(null), 114-431.
https://hdl.handle.net/21.15107/rcub_ibiss_988

Nikolić-Kokić A, Slavić M, Oreščanin Dušić Z, Spasić M, Stević ZD, Rakocević-Stojanović VM, Blagojević D. Erythrocyte SOD1 activity-role in bioavailability of NO and possible cause of 'duing back' phenomena in neurodegenerative diseases. in Febs Journal. 2013;280(null):114-431.
https://hdl.handle.net/21.15107/rcub_ibiss_988 .

Nikolić-Kokić, Aleksandra, Slavić, Marija, Oreščanin Dušić, Zorana, Spasić, Mihajlo, Stević, Zorica D, Rakocević-Stojanović, Vidosava M, Blagojević, Duško, "Erythrocyte SOD1 activity-role in bioavailability of NO and possible cause of 'duing back' phenomena in neurodegenerative diseases" in Febs Journal, 280, no. null (2013):114-431,
https://hdl.handle.net/21.15107/rcub_ibiss_988 .

TY  - JOUR
AU  - Ignjatović, Aleksandar
AU  - Stević, Zorica D
AU  - Lavrnić, Dragana S
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan B
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1178
AB  - ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.
T2  - Amyotrophic Lateral Sclerosis
T1  - Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
IS  - 4
VL  - 13
EP  - 362
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1178
ER  - 

@article{
author = "Ignjatović, Aleksandar and Stević, Zorica D and Lavrnić, Dragana S and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasić, Mihajlo and Spasojević, Ivan B",
year = "2012",
abstract = "ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.",
journal = "Amyotrophic Lateral Sclerosis",
title = "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
number = "4",
volume = "13",
pages = "362",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1178"
}

Ignjatović, A., Stević, Z. D., Lavrnić, D. S., Nikolić-Kokić, A., Blagojević, D., Spasić, M.,& Spasojević, I. B.. (2012). Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis, 13(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1178

Ignjatović A, Stević ZD, Lavrnić DS, Nikolić-Kokić A, Blagojević D, Spasić M, Spasojević IB. Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis. 2012;13(4):null-362.
https://hdl.handle.net/21.15107/rcub_ibiss_1178 .

Ignjatović, Aleksandar, Stević, Zorica D, Lavrnić, Dragana S, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasić, Mihajlo, Spasojević, Ivan B, "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Amyotrophic Lateral Sclerosis, 13, no. 4 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1178 .

TY  - JOUR
AU  - Spasojević, Ivan B
AU  - Morina, Arian
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1405
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
IS  - 1
VL  - 15
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1405
ER  - 

@article{
author = "Spasojević, Ivan B and Morina, Arian and Mojović, Milos D and Stević, Zorica D and Spasić, Snezana D and Jones, David R and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
number = "1",
volume = "15",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1405"
}

Spasojević, I. B., Morina, A., Mojović, M. D., Stević, Z. D., Spasić, S. D., Jones, D. R.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report, 15(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1405

Spasojević IB, Morina A, Mojović MD, Stević ZD, Spasić SD, Jones DR, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):null-35.
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

Spasojević, Ivan B, Morina, Arian, Mojović, Milos D, Stević, Zorica D, Spasić, Snezana D, Jones, David R, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

TY  - JOUR
AU  - Spasojević, Ivan B
AU  - Stević, Zorica D
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1392
AB  - Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
T2  - Redox Report
T1  - Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
IS  - 2
VL  - 15
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1392
ER  - 

@article{
author = "Spasojević, Ivan B and Stević, Zorica D and Nikolić-Kokić, Aleksandra and Jones, David R and Blagojević, Duško and Spasić, Mihajlo",
year = "2010",
abstract = "Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.",
journal = "Redox Report",
title = "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
number = "2",
volume = "15",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1392"
}

Spasojević, I. B., Stević, Z. D., Nikolić-Kokić, A., Jones, D. R., Blagojević, D.,& Spasić, M.. (2010). Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report, 15(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1392

Spasojević IB, Stević ZD, Nikolić-Kokić A, Jones DR, Blagojević D, Spasić M. Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2010;15(2):null-86.
https://hdl.handle.net/21.15107/rcub_ibiss_1392 .

Spasojević, Ivan B, Stević, Zorica D, Nikolić-Kokić, Aleksandra, Jones, David R, Blagojević, Duško, Spasić, Mihajlo, "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 15, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1392 .

TY  - CONF
AU  - Spasojević, Ivan B
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Batas, Valentina
AU  - Bacić, Goran G
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1666
C3  - Free Radical Research
T1  - Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS
IS  - null
VL  - 40
EP  - S90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1666
ER  - 

@conference{
author = "Spasojević, Ivan B and Mojović, Milos D and Stević, Zorica D and Batas, Valentina and Bacić, Goran G and Spasić, Mihajlo",
year = "2006",
journal = "Free Radical Research",
title = "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS",
number = "null",
volume = "40",
pages = "S90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1666"
}

Spasojević, I. B., Mojović, M. D., Stević, Z. D., Batas, V., Bacić, G. G.,& Spasić, M.. (2006). Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research, 40(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1666

Spasojević IB, Mojović MD, Stević ZD, Batas V, Bacić GG, Spasić M. Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research. 2006;40(null):null-S90.
https://hdl.handle.net/21.15107/rcub_ibiss_1666 .

Spasojević, Ivan B, Mojović, Milos D, Stević, Zorica D, Batas, Valentina, Bacić, Goran G, Spasić, Mihajlo, "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS" in Free Radical Research, 40, no. null (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1666 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Stević, Zorica D
AU  - Blagojević, Duško
AU  - Davidović, Biljana
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1647
AB  - Background: Overproduction of nitric oxide (NO) and hydrogen peroxide (H2O2) may be an important factor in the pathogenesis of amyotrophic lateral sclerosis (ALS). Owing to their ability to permeate through biological membranes, excess NO and H2O2 may be present in the media surrounding motor neurones. Anti-oxidative defence enzymes (ADEs) in erythrocytes are capable of detoxifying reactive oxygen species (produced endogenously or exogenously), but may also be structurally modified and inactivated by reactive oxygen and nitrogen species. Both balanced and coordinated ADE activities are of utmost importance for their correct physiological function. Methods: We determined activity of the following ADEs: copper-zinc superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) in erythrocytes from sporadic ALS patients [SALS (-/+)], familial ALS patients with the Leu144Phe mutation in the SOD1 gene [FALS (+/-)], asymptomatic carriers with the Leu144Phe mutation in the SOD1 gene (+/-), and control subjects (-/-). We also examined the in vitro effect of diethyldithiocarbamate (DDC) on CuZn SOD activity in erythrocytes from FALS patients, SALS patients and control subjects. Results: The influence of the Leu144Phe mutation and/or disease was apparent for ADE activities measured in all three patient groups. The SOD1 gene mutation decreased CuZn SOD and GSH-Px activity (two-way ANOVA, significant mutation effect). We noted that the disease also contributed to decreased CuZn SOD activity in SALS patients in comparison with the control group (two-way ANOVA, mutation and disease effect). The disease also influenced CAT and GR activity. CAT activity was decreased in both SALS and FALS patients. In all three patient groups, GR activity was higher than in the control group. Finally, DDC inhibited CuZn SOD activity in erythrocytes from control subjects, FALS (Leu144Phe) patients and SALS patients; however, its effect was more pronounced and significant in FALS patients. Conclusions: Changes in erythrocyte ADE activities suggest that oxidative stress, involved in the motor neurone pathogenesis of SALS and FALS, also has systemic effects. Differences in ADE systems between the study groups revealed the presence of different types of oxidative pressure, indicating the potential additional benefit of individually designed anti-oxidant cocktail therapies.
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients
IS  - 5
VL  - 44
EP  - 593
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1647
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Stević, Zorica D and Blagojević, Duško and Davidović, Biljana and Jones, David R and Spasić, Mihajlo",
year = "2006",
abstract = "Background: Overproduction of nitric oxide (NO) and hydrogen peroxide (H2O2) may be an important factor in the pathogenesis of amyotrophic lateral sclerosis (ALS). Owing to their ability to permeate through biological membranes, excess NO and H2O2 may be present in the media surrounding motor neurones. Anti-oxidative defence enzymes (ADEs) in erythrocytes are capable of detoxifying reactive oxygen species (produced endogenously or exogenously), but may also be structurally modified and inactivated by reactive oxygen and nitrogen species. Both balanced and coordinated ADE activities are of utmost importance for their correct physiological function. Methods: We determined activity of the following ADEs: copper-zinc superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) in erythrocytes from sporadic ALS patients [SALS (-/+)], familial ALS patients with the Leu144Phe mutation in the SOD1 gene [FALS (+/-)], asymptomatic carriers with the Leu144Phe mutation in the SOD1 gene (+/-), and control subjects (-/-). We also examined the in vitro effect of diethyldithiocarbamate (DDC) on CuZn SOD activity in erythrocytes from FALS patients, SALS patients and control subjects. Results: The influence of the Leu144Phe mutation and/or disease was apparent for ADE activities measured in all three patient groups. The SOD1 gene mutation decreased CuZn SOD and GSH-Px activity (two-way ANOVA, significant mutation effect). We noted that the disease also contributed to decreased CuZn SOD activity in SALS patients in comparison with the control group (two-way ANOVA, mutation and disease effect). The disease also influenced CAT and GR activity. CAT activity was decreased in both SALS and FALS patients. In all three patient groups, GR activity was higher than in the control group. Finally, DDC inhibited CuZn SOD activity in erythrocytes from control subjects, FALS (Leu144Phe) patients and SALS patients; however, its effect was more pronounced and significant in FALS patients. Conclusions: Changes in erythrocyte ADE activities suggest that oxidative stress, involved in the motor neurone pathogenesis of SALS and FALS, also has systemic effects. Differences in ADE systems between the study groups revealed the presence of different types of oxidative pressure, indicating the potential additional benefit of individually designed anti-oxidant cocktail therapies.",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients",
number = "5",
volume = "44",
pages = "593",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1647"
}

Nikolić-Kokić, A., Stević, Z. D., Blagojević, D., Davidović, B., Jones, D. R.,& Spasić, M.. (2006). Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients. in Clinical Chemistry and Laboratory Medicine, 44(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1647

Nikolić-Kokić A, Stević ZD, Blagojević D, Davidović B, Jones DR, Spasić M. Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients. in Clinical Chemistry and Laboratory Medicine. 2006;44(5):null-593.
https://hdl.handle.net/21.15107/rcub_ibiss_1647 .

Nikolić-Kokić, Aleksandra, Stević, Zorica D, Blagojević, Duško, Davidović, Biljana, Jones, David R, Spasić, Mihajlo, "Alterations in anti-oxidative defence enzymes in erythrocytes from sporadic amyotrophic lateral sclerosis (SALS) and familial ALS patients" in Clinical Chemistry and Laboratory Medicine, 44, no. 5 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1647 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Stević, Zorica D
AU  - Stojanović, Srđan D.
AU  - Blagojević, Duško
AU  - Jones, David R
AU  - Pavlović, Sanja
AU  - Niketić, Vesna P
AU  - Apostolski, Slobodan A
AU  - Spasić, Mihajlo
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1689
AB  - Recent findings indicate that nitric oxide (NOcenter dot) over-production might be an important factor in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). We measured significantly higher concentrations of uric acid and thiol group-containing molecules (R-SH groups) in the cerebrospinal fluid (CSF) from SALS patients compared to controls. The above factors, together with a slightly increased free iron concentration found in the CSF, favour conditions necessary for the formation of the dinitrosyl iron complex, capable of NOcenter dot bio-transformation. Thus, we performed ex vivo saturation of CSF ( from both SALS patients and controls) with NOcenter dot. A decrease in the level of R - SH was found. This was more pronounced in the CSF from SALS patients. In the CSF from SALS patients the production of nitrite and hydroxylamine was greater than that observed in the CSF from controls. Moreover, we also found increased Cu, Zn-SOD activity in the CSF from SALS patients ( when compared to control subjects) but no activity corresponding to Mn-SOD in any CSF samples. As Cu, Zn-SOD can react with nitroxyl forming NOcenter dot, the conditions for a closed, but continuous, loop of NOcenter dot biotransformation are present in the CSF of ALS patients.
T2  - Redox Report
T1  - Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
IS  - 5
VL  - 10
EP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1689
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Stević, Zorica D and Stojanović, Srđan D. and Blagojević, Duško and Jones, David R and Pavlović, Sanja and Niketić, Vesna P and Apostolski, Slobodan A and Spasić, Mihajlo",
year = "2005",
abstract = "Recent findings indicate that nitric oxide (NOcenter dot) over-production might be an important factor in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). We measured significantly higher concentrations of uric acid and thiol group-containing molecules (R-SH groups) in the cerebrospinal fluid (CSF) from SALS patients compared to controls. The above factors, together with a slightly increased free iron concentration found in the CSF, favour conditions necessary for the formation of the dinitrosyl iron complex, capable of NOcenter dot bio-transformation. Thus, we performed ex vivo saturation of CSF ( from both SALS patients and controls) with NOcenter dot. A decrease in the level of R - SH was found. This was more pronounced in the CSF from SALS patients. In the CSF from SALS patients the production of nitrite and hydroxylamine was greater than that observed in the CSF from controls. Moreover, we also found increased Cu, Zn-SOD activity in the CSF from SALS patients ( when compared to control subjects) but no activity corresponding to Mn-SOD in any CSF samples. As Cu, Zn-SOD can react with nitroxyl forming NOcenter dot, the conditions for a closed, but continuous, loop of NOcenter dot biotransformation are present in the CSF of ALS patients.",
journal = "Redox Report",
title = "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
number = "5",
volume = "10",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1689"
}

Nikolić-Kokić, A., Stević, Z. D., Stojanović, S. D., Blagojević, D., Jones, D. R., Pavlović, S., Niketić, V. P., Apostolski, S. A.,& Spasić, M.. (2005). Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report, 10(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1689

Nikolić-Kokić A, Stević ZD, Stojanović SD, Blagojević D, Jones DR, Pavlović S, Niketić VP, Apostolski SA, Spasić M. Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2005;10(5):null-270.
https://hdl.handle.net/21.15107/rcub_ibiss_1689 .

Nikolić-Kokić, Aleksandra, Stević, Zorica D, Stojanović, Srđan D., Blagojević, Duško, Jones, David R, Pavlović, Sanja, Niketić, Vesna P, Apostolski, Slobodan A, Spasić, Mihajlo, "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 10, no. 5 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1689 .

TY  - CONF
AU  - Nikolić, Aleksandra L.
AU  - Blagojević, Duško
AU  - Stević, Zorica D
AU  - Niketić, Vesna P
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1804
C3  - Free Radical Biology and Medicine
T1  - Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination
IS  - null
VL  - 33
EP  - S126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1804
ER  - 

@conference{
author = "Nikolić, Aleksandra L. and Blagojević, Duško and Stević, Zorica D and Niketić, Vesna P and Saičić, Zorica and Spasić, Mihajlo",
year = "2002",
journal = "Free Radical Biology and Medicine",
title = "Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination",
number = "null",
volume = "33",
pages = "S126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1804"
}

Nikolić, A. L., Blagojević, D., Stević, Z. D., Niketić, V. P., Saičić, Z.,& Spasić, M.. (2002). Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination. in Free Radical Biology and Medicine, 33(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1804

Nikolić AL, Blagojević D, Stević ZD, Niketić VP, Saičić Z, Spasić M. Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination. in Free Radical Biology and Medicine. 2002;33(null):null-S126.
https://hdl.handle.net/21.15107/rcub_ibiss_1804 .

Nikolić, Aleksandra L., Blagojević, Duško, Stević, Zorica D, Niketić, Vesna P, Saičić, Zorica, Spasić, Mihajlo, "Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination" in Free Radical Biology and Medicine, 33, no. null (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1804 .

TY  - JOUR
AU  - Stević, Zorica D
AU  - Nikolić, Aleksandra L.
AU  - Blagojević, Duško
AU  - Saičić, Zorica
AU  - Kocev, Nikola I
AU  - Apostolski, Slobodan A
AU  - Spasić, Mihajlo
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1811
AB  - We assessed a combination of methionine, vitamin E and selenium, as Alsemet(R) in a double blind, placebo controlled trial in 28 out patients with amyotrophic lateral sclerosis. After 12 months, 50% of patients in the placebo group were still alive as compared to 81.3% in the Alsemet(R) group (p<0.05). The rate of deterioration, limb and bulbar-function scores (p<0.05) and muscle-testing score (p<0.025) were significantly lower in the Alsemet(R) group than in the placebo group at the end of a sixth month treatment. We noted a significant increase in GSH-Px activity (p<0.05), as well as an increase in the amount of Vit E (p<0.05) in the Alsemet(R) group in comparison to the placebo group during the clinical trial.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - A controlled trial of combination of methionine and antioxidants in ALS patients
IS  - 4
VL  - 20
EP  - 228
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1811
ER  - 

@article{
author = "Stević, Zorica D and Nikolić, Aleksandra L. and Blagojević, Duško and Saičić, Zorica and Kocev, Nikola I and Apostolski, Slobodan A and Spasić, Mihajlo",
year = "2001",
abstract = "We assessed a combination of methionine, vitamin E and selenium, as Alsemet(R) in a double blind, placebo controlled trial in 28 out patients with amyotrophic lateral sclerosis. After 12 months, 50% of patients in the placebo group were still alive as compared to 81.3% in the Alsemet(R) group (p<0.05). The rate of deterioration, limb and bulbar-function scores (p<0.05) and muscle-testing score (p<0.025) were significantly lower in the Alsemet(R) group than in the placebo group at the end of a sixth month treatment. We noted a significant increase in GSH-Px activity (p<0.05), as well as an increase in the amount of Vit E (p<0.05) in the Alsemet(R) group in comparison to the placebo group during the clinical trial.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "A controlled trial of combination of methionine and antioxidants in ALS patients",
number = "4",
volume = "20",
pages = "228",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1811"
}

Stević, Z. D., Nikolić, A. L., Blagojević, D., Saičić, Z., Kocev, N. I., Apostolski, S. A.,& Spasić, M.. (2001). A controlled trial of combination of methionine and antioxidants in ALS patients. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 20(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1811

Stević ZD, Nikolić AL, Blagojević D, Saičić Z, Kocev NI, Apostolski SA, Spasić M. A controlled trial of combination of methionine and antioxidants in ALS patients. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 2001;20(4):null-228.
https://hdl.handle.net/21.15107/rcub_ibiss_1811 .

Stević, Zorica D, Nikolić, Aleksandra L., Blagojević, Duško, Saičić, Zorica, Kocev, Nikola I, Apostolski, Slobodan A, Spasić, Mihajlo, "A controlled trial of combination of methionine and antioxidants in ALS patients" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 20, no. 4 (2001),
https://hdl.handle.net/21.15107/rcub_ibiss_1811 .