TY  - JOUR
AU  - Appiah, Isabella
AU  - Milovanović, Slobodan R
AU  - Radojičić, Ratko M.
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Slavić, Marija
AU  - Trbojević, Stevan
AU  - Skrbić, Ranko
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1421
AB  - Background and purpose: The effects of hydrogen peroxide (H(2)O(2)) on uterine smooth muscle are not well studied. We have investigated the effect and the mechanism of action of exogenous hydrogen peroxide on rat uteri contractile activity [spontaneous and calcium ion (Ca2+)-induced] and the effect of such treatment on anti-oxidative enzyme activities. Experimental approach: Uteri were isolated from virgin Wistar rats and suspended in an organ bath. Uteri were allowed to contract spontaneously or in the presence of Ca2+ (6 mM) and treated with H(2)O(2) (2 mu M-3 mM) over 2 h. Anti-oxidative enzyme activities (manganese superoxide dismutase-MnSOD, copper-zinc superoxide dismutase-CuZnSOD, catalase-CAT, glutathione peroxidase-GSHPx and glutathione reductase-GR) in H(2)O(2)-treated uteri were compared with those in uteri immediately frozen after isolation or undergoing spontaneous or Ca2+-induced contractions, without treatment with H(2)O(2). The effect of inhibitors (propranolol, methylene blue, L-NAME, tetraethylamonium, glibenclamide and 4-aminopyridine) on H(2)O(2)-mediated relaxation was explored. Key results: H(2)O(2) caused concentration-dependent relaxation of both spontaneous and Ca2+-induced uterine contractions. After H(2)O(2) treatment, GSHPx and MnSOD activities were increased, while CuZnSOD and GR (In Ca2+-induced rat uteri) were decreased. N omega-nitro-L-arginine methyl ester antagonized the effect of H(2)O(2) on Ca2+-induced contractions. H(2)O(2)-induced relaxation was not affected by propranolol, potentiated by methylene blue and antagonized by tetraethylamonium, 4-aminopyridine and glibenclamide, with the last compound being the least effective. Conclusions and implications: H(2)O(2) induced dose-dependent relaxation of isolated rat uteri mainly via changes in voltage-dependent potassium channels. Decreasing generation of reactive oxygen species by stimulation of anti-oxidative pathways may lead to new approaches to the management of dysfunctional uteri.
T2  - British Journal of Pharmacology
T1  - Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus
IS  - 8
VL  - 158
EP  - 1941
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1421
ER  - 

@article{
author = "Appiah, Isabella and Milovanović, Slobodan R and Radojičić, Ratko M. and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Slavić, Marija and Trbojević, Stevan and Skrbić, Ranko and Spasić, Mihajlo and Blagojević, Duško",
year = "2009",
abstract = "Background and purpose: The effects of hydrogen peroxide (H(2)O(2)) on uterine smooth muscle are not well studied. We have investigated the effect and the mechanism of action of exogenous hydrogen peroxide on rat uteri contractile activity [spontaneous and calcium ion (Ca2+)-induced] and the effect of such treatment on anti-oxidative enzyme activities. Experimental approach: Uteri were isolated from virgin Wistar rats and suspended in an organ bath. Uteri were allowed to contract spontaneously or in the presence of Ca2+ (6 mM) and treated with H(2)O(2) (2 mu M-3 mM) over 2 h. Anti-oxidative enzyme activities (manganese superoxide dismutase-MnSOD, copper-zinc superoxide dismutase-CuZnSOD, catalase-CAT, glutathione peroxidase-GSHPx and glutathione reductase-GR) in H(2)O(2)-treated uteri were compared with those in uteri immediately frozen after isolation or undergoing spontaneous or Ca2+-induced contractions, without treatment with H(2)O(2). The effect of inhibitors (propranolol, methylene blue, L-NAME, tetraethylamonium, glibenclamide and 4-aminopyridine) on H(2)O(2)-mediated relaxation was explored. Key results: H(2)O(2) caused concentration-dependent relaxation of both spontaneous and Ca2+-induced uterine contractions. After H(2)O(2) treatment, GSHPx and MnSOD activities were increased, while CuZnSOD and GR (In Ca2+-induced rat uteri) were decreased. N omega-nitro-L-arginine methyl ester antagonized the effect of H(2)O(2) on Ca2+-induced contractions. H(2)O(2)-induced relaxation was not affected by propranolol, potentiated by methylene blue and antagonized by tetraethylamonium, 4-aminopyridine and glibenclamide, with the last compound being the least effective. Conclusions and implications: H(2)O(2) induced dose-dependent relaxation of isolated rat uteri mainly via changes in voltage-dependent potassium channels. Decreasing generation of reactive oxygen species by stimulation of anti-oxidative pathways may lead to new approaches to the management of dysfunctional uteri.",
journal = "British Journal of Pharmacology",
title = "Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus",
number = "8",
volume = "158",
pages = "1941",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1421"
}

Appiah, I., Milovanović, S. R., Radojičić, R. M., Nikolić-Kokić, A., Oreščanin Dušić, Z., Slavić, M., Trbojević, S., Skrbić, R., Spasić, M.,& Blagojević, D.. (2009). Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus. in British Journal of Pharmacology, 158(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1421

Appiah I, Milovanović SR, Radojičić RM, Nikolić-Kokić A, Oreščanin Dušić Z, Slavić M, Trbojević S, Skrbić R, Spasić M, Blagojević D. Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus. in British Journal of Pharmacology. 2009;158(8):null-1941.
https://hdl.handle.net/21.15107/rcub_ibiss_1421 .

Appiah, Isabella, Milovanović, Slobodan R, Radojičić, Ratko M., Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Slavić, Marija, Trbojević, Stevan, Skrbić, Ranko, Spasić, Mihajlo, Blagojević, Duško, "Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus" in British Journal of Pharmacology, 158, no. 8 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1421 .