TY  - CONF
AU  - Ljujić, Mila
AU  - Trifunović, Sara
AU  - Ilić, Bojan
AU  - Milovanović, Jelena
AU  - Dinić, Jelena
AU  - Divac Rankov, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6250
AB  - Introduction: Cigarette smoke exposure is a known risk factor for development of lung diseases and
electronic cigarettes (e-cigarettes) were introduced as a popular and safer alternative to combustible tobacco products. Increasing number of studies are reporting their adverse biological effects both in vivo
and in vitro. Aim of this study was to evaluate the effect of e-cigarettes on mitochondrial function in
lung bronchial epithelial cells.
Methods: Electronic cigarette vapor condensate (ECC) was generated using an e-cigarette device on a
suction trap cooled in a dry ice/ethanol bath. We used unflavoured and flavoured e-cigarette liquids with
and without nicotine. Human bronchial epithelial BEAS2B cells were seeded in 96well plates and treated
with 2% e-cigarette vapour condensate for 24h. Mitochondrial membrane potential was measured using
50nM TMRE (Tetramethyl rhodamine ethyl ester) and cells were visualized on ImageXpress® Pico Automated Cell Imaging System (Molecular Devices, San Jose, CA, USA) with a 10x objective.
Results: We found a significant reduction of TMRE fluorescence in treated cells compared to the control. Imaging of treated cells also revealed changes in cell morphology and the presence of mitochondria in TNT-like structures.
Conclusion: Mitochondrial dysfunction has been associated with various pathological conditions including lung diseases such as asthma, COPD and lung cancer. Due to their relative novelty, the role of
electronic cigarette use in development of chronic lung diseases is still relatively unknown. Our findings
contribute to the growing list of studies pointing to their adverse biological effects and imply their involvement in processes contributing to chronic lung diseases.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cells
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6250
ER  - 

@conference{
author = "Ljujić, Mila and Trifunović, Sara and Ilić, Bojan and Milovanović, Jelena and Dinić, Jelena and Divac Rankov, Aleksandra",
year = "2023",
abstract = "Introduction: Cigarette smoke exposure is a known risk factor for development of lung diseases and
electronic cigarettes (e-cigarettes) were introduced as a popular and safer alternative to combustible tobacco products. Increasing number of studies are reporting their adverse biological effects both in vivo
and in vitro. Aim of this study was to evaluate the effect of e-cigarettes on mitochondrial function in
lung bronchial epithelial cells.
Methods: Electronic cigarette vapor condensate (ECC) was generated using an e-cigarette device on a
suction trap cooled in a dry ice/ethanol bath. We used unflavoured and flavoured e-cigarette liquids with
and without nicotine. Human bronchial epithelial BEAS2B cells were seeded in 96well plates and treated
with 2% e-cigarette vapour condensate for 24h. Mitochondrial membrane potential was measured using
50nM TMRE (Tetramethyl rhodamine ethyl ester) and cells were visualized on ImageXpress® Pico Automated Cell Imaging System (Molecular Devices, San Jose, CA, USA) with a 10x objective.
Results: We found a significant reduction of TMRE fluorescence in treated cells compared to the control. Imaging of treated cells also revealed changes in cell morphology and the presence of mitochondria in TNT-like structures.
Conclusion: Mitochondrial dysfunction has been associated with various pathological conditions including lung diseases such as asthma, COPD and lung cancer. Due to their relative novelty, the role of
electronic cigarette use in development of chronic lung diseases is still relatively unknown. Our findings
contribute to the growing list of studies pointing to their adverse biological effects and imply their involvement in processes contributing to chronic lung diseases.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cells",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6250"
}

Ljujić, M., Trifunović, S., Ilić, B., Milovanović, J., Dinić, J.,& Divac Rankov, A.. (2023). Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cells. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 139.
https://hdl.handle.net/21.15107/rcub_ibiss_6250

Ljujić M, Trifunović S, Ilić B, Milovanović J, Dinić J, Divac Rankov A. Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cells. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:139.
https://hdl.handle.net/21.15107/rcub_ibiss_6250 .

Ljujić, Mila, Trifunović, Sara, Ilić, Bojan, Milovanović, Jelena, Dinić, Jelena, Divac Rankov, Aleksandra, "Electronic cigarette vapour condensate affects mitochondrial potential in BEAS2B cells" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):139,
https://hdl.handle.net/21.15107/rcub_ibiss_6250 .

TY  - JOUR
AU  - Popović, Vladan
AU  - Šešlija Jovanović, Darka
AU  - Miletić, Zoran
AU  - Milovanović, Jelena
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Miljković, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5232
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5248
AB  - The aim of this research was to quantifythe content of hazardous elements in the needles ofNorway spruce (Picea abies L.) in the natural habitatsthat were accumulated from thermal power plants,mines, and metal processing industry. Fifteen naturalpopulations of the Norway spruce were sampled fromthe mountain ranges in Southeastern Europe (DinaricAlps and Balkan Mountains). Two-year-old spruceneedles were evaluated the content of the followinghazardous elements: heavy metals cadmium, mercury,nickel, lead and zinc, and metalloid arsenic. The effectof the distance between air pollution emitters and theNorway spruce natural habitats on the hazardous elementscontent in needles was also evaluated. Theresults of the analysis of variance confirmed interpopulationdifferences in the content of all analyzed hazardous elements. The effect of the air pollutionsource (thermal power plants, mines, and industry) onthe content of hazardous elements in the spruce needleswas also assessed. Significant correlation wasfound between the distance of air pollution emittersand the amount of zinc. This study could serve as thestartup point of future monitoring programs and providenew prospect of using Norway spruce needlesas the bioindicator of air pollution with hazardouselements on Balkan Peninsula since the fact that theNorway spruce natural populations inhabit wide geographicrange of the continental Europe, from the BalkanPeninsula, over European Alps to Scandinavia anda large-scale of altitude from 980 to 1860 m above sealevel.
PB  - Springer Nature
T2  - Environmental Monitoring and Assessment
T1  - The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats
IS  - 109
VL  - 195
DO  - 10.1007/s10661-022-10732-2
ER  - 

@article{
author = "Popović, Vladan and Šešlija Jovanović, Darka and Miletić, Zoran and Milovanović, Jelena and Lučić, Aleksandar and Rakonjac, Ljubinko and Miljković, Danijela",
year = "2023",
abstract = "The aim of this research was to quantifythe content of hazardous elements in the needles ofNorway spruce (Picea abies L.) in the natural habitatsthat were accumulated from thermal power plants,mines, and metal processing industry. Fifteen naturalpopulations of the Norway spruce were sampled fromthe mountain ranges in Southeastern Europe (DinaricAlps and Balkan Mountains). Two-year-old spruceneedles were evaluated the content of the followinghazardous elements: heavy metals cadmium, mercury,nickel, lead and zinc, and metalloid arsenic. The effectof the distance between air pollution emitters and theNorway spruce natural habitats on the hazardous elementscontent in needles was also evaluated. Theresults of the analysis of variance confirmed interpopulationdifferences in the content of all analyzed hazardous elements. The effect of the air pollutionsource (thermal power plants, mines, and industry) onthe content of hazardous elements in the spruce needleswas also assessed. Significant correlation wasfound between the distance of air pollution emittersand the amount of zinc. This study could serve as thestartup point of future monitoring programs and providenew prospect of using Norway spruce needlesas the bioindicator of air pollution with hazardouselements on Balkan Peninsula since the fact that theNorway spruce natural populations inhabit wide geographicrange of the continental Europe, from the BalkanPeninsula, over European Alps to Scandinavia anda large-scale of altitude from 980 to 1860 m above sealevel.",
publisher = "Springer Nature",
journal = "Environmental Monitoring and Assessment",
title = "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats",
number = "109",
volume = "195",
doi = "10.1007/s10661-022-10732-2"
}

Popović, V., Šešlija Jovanović, D., Miletić, Z., Milovanović, J., Lučić, A., Rakonjac, L.,& Miljković, D.. (2023). The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment
Springer Nature., 195(109).
https://doi.org/10.1007/s10661-022-10732-2

Popović V, Šešlija Jovanović D, Miletić Z, Milovanović J, Lučić A, Rakonjac L, Miljković D. The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment. 2023;195(109).
doi:10.1007/s10661-022-10732-2 .

Popović, Vladan, Šešlija Jovanović, Darka, Miletić, Zoran, Milovanović, Jelena, Lučić, Aleksandar, Rakonjac, Ljubinko, Miljković, Danijela, "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats" in Environmental Monitoring and Assessment, 195, no. 109 (2023),
https://doi.org/10.1007/s10661-022-10732-2 . .

TY  - JOUR
AU  - Popović, Vladan
AU  - Šešlija Jovanović, Darka
AU  - Miletić, Zoran
AU  - Milovanović, Jelena
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Miljković, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5232
AB  - The aim of this research was to quantify
the content of hazardous elements in the needles of
Norway spruce (Picea abies L.) in the natural habitats
that were accumulated from thermal power plants,
mines, and metal processing industry. Fifteen natural
populations of the Norway spruce were sampled from
the mountain ranges in Southeastern Europe (Dinaric
Alps and Balkan Mountains). Two-year-old spruce
needles were evaluated the content of the following
hazardous elements: heavy metals cadmium, mercury,
nickel, lead and zinc, and metalloid arsenic. The effect
of the distance between air pollution emitters and the
Norway spruce natural habitats on the hazardous elements
content in needles was also evaluated. The
results of the analysis of variance confirmed interpopulation
differences in the content of all analyzed hazardous elements. The effect of the air pollution
source (thermal power plants, mines, and industry) on
the content of hazardous elements in the spruce needles
was also assessed. Significant correlation was
found between the distance of air pollution emitters
and the amount of zinc. This study could serve as the
startup point of future monitoring programs and provide
new prospect of using Norway spruce needles
as the bioindicator of air pollution with hazardous
elements on Balkan Peninsula since the fact that the
Norway spruce natural populations inhabit wide geographic
range of the continental Europe, from the Balkan
Peninsula, over European Alps to Scandinavia and
a large-scale of altitude from 980 to 1860 m above sea
level.
PB  - Springer Nature
T2  - Environmental Monitoring and Assessment
T1  - The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats
IS  - 109
VL  - 195
DO  - 10.1007/s10661-022-10732-2
ER  - 

@article{
author = "Popović, Vladan and Šešlija Jovanović, Darka and Miletić, Zoran and Milovanović, Jelena and Lučić, Aleksandar and Rakonjac, Ljubinko and Miljković, Danijela",
year = "2023",
abstract = "The aim of this research was to quantify
the content of hazardous elements in the needles of
Norway spruce (Picea abies L.) in the natural habitats
that were accumulated from thermal power plants,
mines, and metal processing industry. Fifteen natural
populations of the Norway spruce were sampled from
the mountain ranges in Southeastern Europe (Dinaric
Alps and Balkan Mountains). Two-year-old spruce
needles were evaluated the content of the following
hazardous elements: heavy metals cadmium, mercury,
nickel, lead and zinc, and metalloid arsenic. The effect
of the distance between air pollution emitters and the
Norway spruce natural habitats on the hazardous elements
content in needles was also evaluated. The
results of the analysis of variance confirmed interpopulation
differences in the content of all analyzed hazardous elements. The effect of the air pollution
source (thermal power plants, mines, and industry) on
the content of hazardous elements in the spruce needles
was also assessed. Significant correlation was
found between the distance of air pollution emitters
and the amount of zinc. This study could serve as the
startup point of future monitoring programs and provide
new prospect of using Norway spruce needles
as the bioindicator of air pollution with hazardous
elements on Balkan Peninsula since the fact that the
Norway spruce natural populations inhabit wide geographic
range of the continental Europe, from the Balkan
Peninsula, over European Alps to Scandinavia and
a large-scale of altitude from 980 to 1860 m above sea
level.",
publisher = "Springer Nature",
journal = "Environmental Monitoring and Assessment",
title = "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats",
number = "109",
volume = "195",
doi = "10.1007/s10661-022-10732-2"
}

Popović, V., Šešlija Jovanović, D., Miletić, Z., Milovanović, J., Lučić, A., Rakonjac, L.,& Miljković, D.. (2023). The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment
Springer Nature., 195(109).
https://doi.org/10.1007/s10661-022-10732-2

Popović V, Šešlija Jovanović D, Miletić Z, Milovanović J, Lučić A, Rakonjac L, Miljković D. The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment. 2023;195(109).
doi:10.1007/s10661-022-10732-2 .

Popović, Vladan, Šešlija Jovanović, Darka, Miletić, Zoran, Milovanović, Jelena, Lučić, Aleksandar, Rakonjac, Ljubinko, Miljković, Danijela, "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats" in Environmental Monitoring and Assessment, 195, no. 109 (2023),
https://doi.org/10.1007/s10661-022-10732-2 . .