TY  - CONF
AU  - Tanić, Nikola
AU  - Blagojević, Danijela
AU  - Milanović, Ivana
AU  - Imširović, Mirela
AU  - Lazić, Sandra
AU  - Maksimović, Zlatko
AU  - Tanić, Nasta
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6328
AB  - Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and caused COVID-19 pandemic. This coronavirus disease pandemic demonstrated the importance of diagnostic testing in disease outbreak monitoring and control. So, reliable and accurate testing for SARS-CoV-2 was the principal prerequisite for preventing the spread of COVID-19.
Methods: Real Time RT-PCR (RT-qPCR) unquestionably represent the most reliable, rapid and sensitive method for detection of SARS-CoV-2 RNA. However, there are numerous different assays, protocols, reagents, instruments and result analysis methods in use without certified standards, standardized RNA extraction and reporting procedures. Therefore, in practice, the reliability of RT-qPCR results depends on a number of parameters that include sample collection and processing, method of RNA extraction, choice of assay, efficiency of assay, choice of instrument, analysis method as well as operator intervention.
Results: Here we present comparative analyses of the efficiency and sensitivity of 10 different amplification assays, as well as the relevance of manual RNA extractions compared to automatic one. Our results revealed that manual viral RNA extraction should be a method of choice for high sensitivity. In addition, amplification assays targeting three SARS-CoV-2 genes are much more efficient from those targeting one.
Conclusion: Unfortunately, RT-qPCR is almost exclusively used as qualitative diagnostic test for SARSCoV-2. We think that the ideal testing regimen would involve not just qualitative detection of SARS-CoV-2 but reliable and meaningful quantitative reporting of viral load.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Diagnostic testing for SARS-COV-2 by Real-time RT-PCR
IS  - 99
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6328
ER  - 

@conference{
author = "Tanić, Nikola and Blagojević, Danijela and Milanović, Ivana and Imširović, Mirela and Lazić, Sandra and Maksimović, Zlatko and Tanić, Nasta",
year = "2023",
abstract = "Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and caused COVID-19 pandemic. This coronavirus disease pandemic demonstrated the importance of diagnostic testing in disease outbreak monitoring and control. So, reliable and accurate testing for SARS-CoV-2 was the principal prerequisite for preventing the spread of COVID-19.
Methods: Real Time RT-PCR (RT-qPCR) unquestionably represent the most reliable, rapid and sensitive method for detection of SARS-CoV-2 RNA. However, there are numerous different assays, protocols, reagents, instruments and result analysis methods in use without certified standards, standardized RNA extraction and reporting procedures. Therefore, in practice, the reliability of RT-qPCR results depends on a number of parameters that include sample collection and processing, method of RNA extraction, choice of assay, efficiency of assay, choice of instrument, analysis method as well as operator intervention.
Results: Here we present comparative analyses of the efficiency and sensitivity of 10 different amplification assays, as well as the relevance of manual RNA extractions compared to automatic one. Our results revealed that manual viral RNA extraction should be a method of choice for high sensitivity. In addition, amplification assays targeting three SARS-CoV-2 genes are much more efficient from those targeting one.
Conclusion: Unfortunately, RT-qPCR is almost exclusively used as qualitative diagnostic test for SARSCoV-2. We think that the ideal testing regimen would involve not just qualitative detection of SARS-CoV-2 but reliable and meaningful quantitative reporting of viral load.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Diagnostic testing for SARS-COV-2 by Real-time RT-PCR",
number = "99",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6328"
}

Tanić, N., Blagojević, D., Milanović, I., Imširović, M., Lazić, S., Maksimović, Z.,& Tanić, N.. (2023). Diagnostic testing for SARS-COV-2 by Real-time RT-PCR. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(99).
https://hdl.handle.net/21.15107/rcub_ibiss_6328

Tanić N, Blagojević D, Milanović I, Imširović M, Lazić S, Maksimović Z, Tanić N. Diagnostic testing for SARS-COV-2 by Real-time RT-PCR. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;(99).
https://hdl.handle.net/21.15107/rcub_ibiss_6328 .

Tanić, Nikola, Blagojević, Danijela, Milanović, Ivana, Imširović, Mirela, Lazić, Sandra, Maksimović, Zlatko, Tanić, Nasta, "Diagnostic testing for SARS-COV-2 by Real-time RT-PCR" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia, no. 99 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6328 .

TY  - JOUR
AU  - Prvanović, Mirjana
AU  - Nedeljković, Milica
AU  - Tanić, Nasta
AU  - Tomić, Tijana
AU  - Terzić, Tanja
AU  - Milovanović, Zorka
AU  - Maksimović, Zlatko
AU  - Tanić, Nikola
PY  - 2021
UR  - https://www.mdpi.com/2075-1729/11/11/1247
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8621563
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4698
AB  - Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive subtype and is associated with high recurrence rates, high incidence of distant metastases, and poor overall survival. The aim of this study was to investigate the PI3K/PTEN/Akt/mTOR pathway as one of the most frequently deregulated pathways in cancer. We aimed to explore the impact of PI3K and mTOR oncogenes as well as the PTEN tumor suppressor on TNBC clinical behavior, prognosis, and multidrug resistance (MDR), using immunohistochemistry and copy number analysis by quantitative real-time PCR. Our results revealed that loss of PTEN and high expression of PI3K and mTOR proteins are associated with poor outcome of TNBC patients. PTEN deletions appeared as a major cause of reduced or absent PTEN expression in TNBC. Importantly, homozygous deletions of PTEN (and not hemizygous deletions) are a potential molecular marker of metastasis formation and good predictors of TNBC outcome. In conclusion, we believe that concurrent examination of PTEN/PI3K/mTOR protein expression may be more useful in predicting TNBC clinical course than the analysis of single protein expression. Specifically, our results showed that PTEN-reduced/PI3K-high/mTOR-high expression constitutes a 'high risk' profile of TNBC.
PB  - Basel: MDPI
T2  - Life (Basel, Switzerland)
T1  - Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.
IS  - 11
VL  - 11
DO  - 10.3390/life11111247
SP  - 1247
ER  - 

@article{
author = "Prvanović, Mirjana and Nedeljković, Milica and Tanić, Nasta and Tomić, Tijana and Terzić, Tanja and Milovanović, Zorka and Maksimović, Zlatko and Tanić, Nikola",
year = "2021",
abstract = "Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive subtype and is associated with high recurrence rates, high incidence of distant metastases, and poor overall survival. The aim of this study was to investigate the PI3K/PTEN/Akt/mTOR pathway as one of the most frequently deregulated pathways in cancer. We aimed to explore the impact of PI3K and mTOR oncogenes as well as the PTEN tumor suppressor on TNBC clinical behavior, prognosis, and multidrug resistance (MDR), using immunohistochemistry and copy number analysis by quantitative real-time PCR. Our results revealed that loss of PTEN and high expression of PI3K and mTOR proteins are associated with poor outcome of TNBC patients. PTEN deletions appeared as a major cause of reduced or absent PTEN expression in TNBC. Importantly, homozygous deletions of PTEN (and not hemizygous deletions) are a potential molecular marker of metastasis formation and good predictors of TNBC outcome. In conclusion, we believe that concurrent examination of PTEN/PI3K/mTOR protein expression may be more useful in predicting TNBC clinical course than the analysis of single protein expression. Specifically, our results showed that PTEN-reduced/PI3K-high/mTOR-high expression constitutes a 'high risk' profile of TNBC.",
publisher = "Basel: MDPI",
journal = "Life (Basel, Switzerland)",
title = "Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.",
number = "11",
volume = "11",
doi = "10.3390/life11111247",
pages = "1247"
}

Prvanović, M., Nedeljković, M., Tanić, N., Tomić, T., Terzić, T., Milovanović, Z., Maksimović, Z.,& Tanić, N.. (2021). Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.. in Life (Basel, Switzerland)
Basel: MDPI., 11(11), 1247.
https://doi.org/10.3390/life11111247

Prvanović M, Nedeljković M, Tanić N, Tomić T, Terzić T, Milovanović Z, Maksimović Z, Tanić N. Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer.. in Life (Basel, Switzerland). 2021;11(11):1247.
doi:10.3390/life11111247 .

Prvanović, Mirjana, Nedeljković, Milica, Tanić, Nasta, Tomić, Tijana, Terzić, Tanja, Milovanović, Zorka, Maksimović, Zlatko, Tanić, Nikola, "Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer." in Life (Basel, Switzerland), 11, no. 11 (2021):1247,
https://doi.org/10.3390/life11111247 . .