TY  - JOUR
AU  - Immonen, Elina
AU  - Sayadi, Ahmed
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Liljestrand-Rönn, Johanna
AU  - Wiberg, Axel
AU  - Arnqvist, Göran
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5378
AB  - The patterns of reproductive timing and senescence vary within and across species owing to differences in reproductive strategies, but our understanding of the molecular underpinnings of such variation is incomplete. This is perhaps particularly true for sex differences. We investigated the evolution of sex-specific gene expression associated with life history divergence in replicated populations of the seed beetle Acanthoscelides obtectus, experimentally evolving under (E)arly or (L)ate life reproduction for >200 generations which has resulted in strongly divergent life histories. We detected 1,646 genes that were differentially expressed in E and L lines, consistent with a highly polygenic basis of life history evolution. Only 30% of differentially expressed genes were similarly affected in males and females. The evolution of long life was associated with significantly reduced sex differences in expression, especially in non-reproductive tissues. The expression differences were overall more pronounced in females, in accordance with their greater phenotypic divergence in lifespan. Functional enrichment analysis revealed differences between E and L beetles in gene categories previously implicated in aging, such as mitochondrial function and defense response. The results show that divergent life history evolution can be associated with profound changes in gene expression that alter the transcriptome in a sex-specific way, highlighting the importance of understanding the mechanisms of aging in each sex.
PB  - Oxford, UK: Oxford University Press
T2  - Genome Biology and Evolution
T1  - Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles
IS  - 1
VL  - 15
DO  - 10.1093/gbe/evac177
SP  - evac177
ER  - 

@article{
author = "Immonen, Elina and Sayadi, Ahmed and Stojković, Biljana and Savković, Uroš and Đorđević, Mirko and Liljestrand-Rönn, Johanna and Wiberg, Axel and Arnqvist, Göran",
year = "2023",
abstract = "The patterns of reproductive timing and senescence vary within and across species owing to differences in reproductive strategies, but our understanding of the molecular underpinnings of such variation is incomplete. This is perhaps particularly true for sex differences. We investigated the evolution of sex-specific gene expression associated with life history divergence in replicated populations of the seed beetle Acanthoscelides obtectus, experimentally evolving under (E)arly or (L)ate life reproduction for >200 generations which has resulted in strongly divergent life histories. We detected 1,646 genes that were differentially expressed in E and L lines, consistent with a highly polygenic basis of life history evolution. Only 30% of differentially expressed genes were similarly affected in males and females. The evolution of long life was associated with significantly reduced sex differences in expression, especially in non-reproductive tissues. The expression differences were overall more pronounced in females, in accordance with their greater phenotypic divergence in lifespan. Functional enrichment analysis revealed differences between E and L beetles in gene categories previously implicated in aging, such as mitochondrial function and defense response. The results show that divergent life history evolution can be associated with profound changes in gene expression that alter the transcriptome in a sex-specific way, highlighting the importance of understanding the mechanisms of aging in each sex.",
publisher = "Oxford, UK: Oxford University Press",
journal = "Genome Biology and Evolution",
title = "Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles",
number = "1",
volume = "15",
doi = "10.1093/gbe/evac177",
pages = "evac177"
}

Immonen, E., Sayadi, A., Stojković, B., Savković, U., Đorđević, M., Liljestrand-Rönn, J., Wiberg, A.,& Arnqvist, G.. (2023). Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles. in Genome Biology and Evolution
Oxford, UK: Oxford University Press., 15(1), evac177.
https://doi.org/10.1093/gbe/evac177

Immonen E, Sayadi A, Stojković B, Savković U, Đorđević M, Liljestrand-Rönn J, Wiberg A, Arnqvist G. Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles. in Genome Biology and Evolution. 2023;15(1):evac177.
doi:10.1093/gbe/evac177 .

Immonen, Elina, Sayadi, Ahmed, Stojković, Biljana, Savković, Uroš, Đorđević, Mirko, Liljestrand-Rönn, Johanna, Wiberg, Axel, Arnqvist, Göran, "Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles" in Genome Biology and Evolution, 15, no. 1 (2023):evac177,
https://doi.org/10.1093/gbe/evac177 . .

TY  - JOUR
AU  - Martinossi-Allibert, Ivain
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Arnqvist, Göran
AU  - Stojković, Biljana
AU  - Berger, David
PY  - 2018
UR  - http://doi.wiley.com/10.1111/evo.13412
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29238970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2963
AB  - Whether sexual selection generally promotes or impedes population persistence remains an open question. Intralocus sexual conflict (IaSC) can render sexual selection in males detrimental to the population by increasing the frequency of alleles with positive effects on male reproductive success but negative effects on female fecundity. Recent modeling based on fitness landscape theory, however, indicates that the relative impact of IaSC may be reduced in maladapted populations and that sexual selection therefore might promote adaptation when it is most needed. Here, we test this prediction using bean beetles that had undergone 80 generations of experimental evolution on two alternative host plants. We isolated and assessed the effect of maladaptation on sex-specific strengths of selection and IaSC by cross-rearing the two experimental evolution regimes on the alternative hosts and estimating within-population genetic (co)variance for fitness in males and females. Two key predictions were upheld: males generally experienced stronger selection compared to females and maladaptation increased selection in females. However, maladaptation consistently decreased male-bias in the strength of selection and IaSC was not reduced in maladapted populations. These findings imply that sexual selection can be disrupted in stressful environmental conditions, thus reducing one of the potential benefits of sexual reproduction in maladapted populations.
T2  - Evolution
T1  - The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.
DO  - 10.1111/evo.13412
ER  - 

@article{
author = "Martinossi-Allibert, Ivain and Savković, Uroš and Đorđević, Mirko and Arnqvist, Göran and Stojković, Biljana and Berger, David",
year = "2018",
abstract = "Whether sexual selection generally promotes or impedes population persistence remains an open question. Intralocus sexual conflict (IaSC) can render sexual selection in males detrimental to the population by increasing the frequency of alleles with positive effects on male reproductive success but negative effects on female fecundity. Recent modeling based on fitness landscape theory, however, indicates that the relative impact of IaSC may be reduced in maladapted populations and that sexual selection therefore might promote adaptation when it is most needed. Here, we test this prediction using bean beetles that had undergone 80 generations of experimental evolution on two alternative host plants. We isolated and assessed the effect of maladaptation on sex-specific strengths of selection and IaSC by cross-rearing the two experimental evolution regimes on the alternative hosts and estimating within-population genetic (co)variance for fitness in males and females. Two key predictions were upheld: males generally experienced stronger selection compared to females and maladaptation increased selection in females. However, maladaptation consistently decreased male-bias in the strength of selection and IaSC was not reduced in maladapted populations. These findings imply that sexual selection can be disrupted in stressful environmental conditions, thus reducing one of the potential benefits of sexual reproduction in maladapted populations.",
journal = "Evolution",
title = "The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.",
doi = "10.1111/evo.13412"
}

Martinossi-Allibert, I., Savković, U., Đorđević, M., Arnqvist, G., Stojković, B.,& Berger, D.. (2018). The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.. in Evolution.
https://doi.org/10.1111/evo.13412

Martinossi-Allibert I, Savković U, Đorđević M, Arnqvist G, Stojković B, Berger D. The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.. in Evolution. 2018;.
doi:10.1111/evo.13412 .

Martinossi-Allibert, Ivain, Savković, Uroš, Đorđević, Mirko, Arnqvist, Göran, Stojković, Biljana, Berger, David, "The consequences of sexual selection in well-adapted and maladapted populations of bean beetles." in Evolution (2018),
https://doi.org/10.1111/evo.13412 . .

TY  - JOUR
AU  - Arnqvist, Göran
AU  - Stojković, Biljana
AU  - Rönn, Johanna L.
AU  - Immonen, Elina
AU  - White, Craig
PY  - 2017
UR  - http://doi.wiley.com/10.1111/1365-2435.12927
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2820
AB  - Metabolic rate (MR) is a key functional trait simply because metabolism converts resources into population growth rate. Yet, our empirical understanding of the sources of within species variation in MR, as well as of its life history and ecological correlates, is rather limited. Here, we assess whether MR lies at the root of a syndrome of correlated rate-dependent life-history traits in an insect. Selection for early (E) or late (L) age-at-reproduction for > 160 generations in the bean beetle Acanthoscelides obtectus has produced beetles that differ markedly in juvenile development, body size, fecundity schedules, ageing and life span. Here, we use micro-respirometry to test whether this has been associated with the evolution of age- and sex-specific metabolic phenotypes. We find that mass-specific MR is 18% higher in E lines compared to L lines and that MR decreases more rapidly with chronological, but not biological, age in E lines. Males, under sexual selection to "live-fast-die-young", show 50% higher MR than females and MR decreased more rapidly with age in males. Our results are consistent with a central role for MR for the divergence in "pace-of-life" seen in these beetles, supporting the view that MR lies at the root of ecologically relevant life-history trait variation within species.
T2  - Functional Ecology
T1  - The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles
DO  - 10.1111/1365-2435.12927
ER  - 

@article{
author = "Arnqvist, Göran and Stojković, Biljana and Rönn, Johanna L. and Immonen, Elina and White, Craig",
year = "2017",
abstract = "Metabolic rate (MR) is a key functional trait simply because metabolism converts resources into population growth rate. Yet, our empirical understanding of the sources of within species variation in MR, as well as of its life history and ecological correlates, is rather limited. Here, we assess whether MR lies at the root of a syndrome of correlated rate-dependent life-history traits in an insect. Selection for early (E) or late (L) age-at-reproduction for > 160 generations in the bean beetle Acanthoscelides obtectus has produced beetles that differ markedly in juvenile development, body size, fecundity schedules, ageing and life span. Here, we use micro-respirometry to test whether this has been associated with the evolution of age- and sex-specific metabolic phenotypes. We find that mass-specific MR is 18% higher in E lines compared to L lines and that MR decreases more rapidly with chronological, but not biological, age in E lines. Males, under sexual selection to "live-fast-die-young", show 50% higher MR than females and MR decreased more rapidly with age in males. Our results are consistent with a central role for MR for the divergence in "pace-of-life" seen in these beetles, supporting the view that MR lies at the root of ecologically relevant life-history trait variation within species.",
journal = "Functional Ecology",
title = "The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles",
doi = "10.1111/1365-2435.12927"
}

Arnqvist, G., Stojković, B., Rönn, J. L., Immonen, E.,& White, C.. (2017). The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles. in Functional Ecology.
https://doi.org/10.1111/1365-2435.12927

Arnqvist G, Stojković B, Rönn JL, Immonen E, White C. The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles. in Functional Ecology. 2017;.
doi:10.1111/1365-2435.12927 .

Arnqvist, Göran, Stojković, Biljana, Rönn, Johanna L., Immonen, Elina, White, Craig, "The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles" in Functional Ecology (2017),
https://doi.org/10.1111/1365-2435.12927 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Sayadi, Ahmed
AU  - Đorđević, Mirko
AU  - Jović, Jelena
AU  - Savković, Uroš
AU  - Arnqvist, Göran
PY  - 2017
UR  - http://doi.wiley.com/10.1111/evo.13102
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2550
AB  - Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.
T2  - Evolution
T1  - Divergent evolution of life span associated with mitochondrial DNA evolution
IS  - 1
VL  - 71
DO  - 10.1111/evo.13102
SP  - 160
EP  - 166
ER  - 

@article{
author = "Stojković, Biljana and Sayadi, Ahmed and Đorđević, Mirko and Jović, Jelena and Savković, Uroš and Arnqvist, Göran",
year = "2017",
abstract = "Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.",
journal = "Evolution",
title = "Divergent evolution of life span associated with mitochondrial DNA evolution",
number = "1",
volume = "71",
doi = "10.1111/evo.13102",
pages = "160-166"
}

Stojković, B., Sayadi, A., Đorđević, M., Jović, J., Savković, U.,& Arnqvist, G.. (2017). Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution, 71(1), 160-166.
https://doi.org/10.1111/evo.13102

Stojković B, Sayadi A, Đorđević M, Jović J, Savković U, Arnqvist G. Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution. 2017;71(1):160-166.
doi:10.1111/evo.13102 .

Stojković, Biljana, Sayadi, Ahmed, Đorđević, Mirko, Jović, Jelena, Savković, Uroš, Arnqvist, Göran, "Divergent evolution of life span associated with mitochondrial DNA evolution" in Evolution, 71, no. 1 (2017):160-166,
https://doi.org/10.1111/evo.13102 . .

TY  - JOUR
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Immonen, Elina
AU  - Tucić, Nikola
AU  - Lazarević, Jelica
AU  - Arnqvist, Göran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2600
AB  - The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
T2  - Evolution
T1  - Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles
IS  - 2
VL  - 71
DO  - 10.1111/evo.13109
SP  - 274
EP  - 288
ER  - 

@article{
author = "Đorđević, Mirko and Stojković, Biljana and Savković, Uroš and Immonen, Elina and Tucić, Nikola and Lazarević, Jelica and Arnqvist, Göran",
year = "2017",
abstract = "The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.",
journal = "Evolution",
title = "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles",
number = "2",
volume = "71",
doi = "10.1111/evo.13109",
pages = "274-288"
}

Đorđević, M., Stojković, B., Savković, U., Immonen, E., Tucić, N., Lazarević, J.,& Arnqvist, G.. (2017). Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution, 71(2), 274-288.
https://doi.org/10.1111/evo.13109

Đorđević M, Stojković B, Savković U, Immonen E, Tucić N, Lazarević J, Arnqvist G. Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution. 2017;71(2):274-288.
doi:10.1111/evo.13109 .

Đorđević, Mirko, Stojković, Biljana, Savković, Uroš, Immonen, Elina, Tucić, Nikola, Lazarević, Jelica, Arnqvist, Göran, "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles" in Evolution, 71, no. 2 (2017):274-288,
https://doi.org/10.1111/evo.13109 . .

TY  - JOUR
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Immonen, Elina
AU  - Tucić, Nikola
AU  - Lazarević, Jelica
AU  - Arnqvist, Göran
PY  - 2016
UR  - http://doi.wiley.com/10.1111/evo.13109
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2549
AB  - The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
T2  - Evolution
T1  - Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles
DO  - 10.1111/evo.13109
ER  - 

@article{
author = "Đorđević, Mirko and Stojković, Biljana and Savković, Uroš and Immonen, Elina and Tucić, Nikola and Lazarević, Jelica and Arnqvist, Göran",
year = "2016",
abstract = "The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.",
journal = "Evolution",
title = "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles",
doi = "10.1111/evo.13109"
}

Đorđević, M., Stojković, B., Savković, U., Immonen, E., Tucić, N., Lazarević, J.,& Arnqvist, G.. (2016). Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution.
https://doi.org/10.1111/evo.13109

Đorđević M, Stojković B, Savković U, Immonen E, Tucić N, Lazarević J, Arnqvist G. Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution. 2016;.
doi:10.1111/evo.13109 .

Đorđević, Mirko, Stojković, Biljana, Savković, Uroš, Immonen, Elina, Tucić, Nikola, Lazarević, Jelica, Arnqvist, Göran, "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles" in Evolution (2016),
https://doi.org/10.1111/evo.13109 . .