TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nađa
AU  - Toljić, Boško
AU  - Arambašić Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3945
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
DO  - 10.1016/j.archoralbio.2020.104929
SP  - 104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nađa and Toljić, Boško and Arambašić Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
doi = "10.1016/j.archoralbio.2020.104929",
pages = "104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Ltd., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nađa, Toljić, Boško, Arambašić Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Pucar, Ana
AU  - Jotić, Aleksandra
AU  - Miličić, Biljana
AU  - Arambašić Jovanović, Jelena
AU  - Vidaković, Melita
AU  - Leković, Vojislav
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6315
AB  - Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity.
Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics.
Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA).
Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000).
Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.
PB  - Belgrade: Serbian Medical Society
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study
IS  - 5-6
VL  - 144
DO  - 10.2298/SARH1606266M
SP  - 266
EP  - 272
ER  - 

@article{
author = "Matić Petrović, Sanja and Pucar, Ana and Jotić, Aleksandra and Miličić, Biljana and Arambašić Jovanović, Jelena and Vidaković, Melita and Leković, Vojislav",
year = "2016",
abstract = "Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity.
Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics.
Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA).
Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000).
Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.",
publisher = "Belgrade: Serbian Medical Society",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study",
number = "5-6",
volume = "144",
doi = "10.2298/SARH1606266M",
pages = "266-272"
}

Matić Petrović, S., Pucar, A., Jotić, A., Miličić, B., Arambašić Jovanović, J., Vidaković, M.,& Leković, V.. (2016). Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo
Belgrade: Serbian Medical Society., 144(5-6), 266-272.
https://doi.org/10.2298/SARH1606266M

Matić Petrović S, Pucar A, Jotić A, Miličić B, Arambašić Jovanović J, Vidaković M, Leković V. Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):266-272.
doi:10.2298/SARH1606266M .

Matić Petrović, Sanja, Pucar, Ana, Jotić, Aleksandra, Miličić, Biljana, Arambašić Jovanović, Jelena, Vidaković, Melita, Leković, Vojislav, "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):266-272,
https://doi.org/10.2298/SARH1606266M . .

TY  - JOUR
AU  - Mangano, Katia
AU  - Fagone, Paolo
AU  - Di Mauro, M
AU  - Ascione, E
AU  - Maiello, V
AU  - Milicić, Tanja
AU  - Jotić, Aleksandra Z
AU  - Lalić, Nebojsa M
AU  - Saksida, Tamara
AU  - Stojanović, Ivana D.
AU  - Selmi, C
AU  - Farina, C
AU  - Stošić-Grujičić, Stanislava
AU  - Meroni, P
AU  - Nicoletti, Ferdinando
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1119
AB  - The transferrin (Tf) family of iron binding proteins includes important endogenous modulators of the immune function that may modulate autoimmune diseases. To define more clearly the role of apotransferrin (apoTf) in type 1 diabetes we determined the impact of this protein on type 1 diabetes as investigated in islet cells, animal models and patient sera. First, we demonstrated that recombinant apoTf counteracts the cytokine-induced death of murine pancreatic islet cells. Secondly, human apoTf administration favourably influences the course of type 1 diabetes in animal models, resulting in protection against disease development that was associated with reduction of insulitis and reduced levels of proinflammatory cytokines. Finally, we confirmed that patients with newly diagnosed type 1 diabetes manifest significantly lower apoTf serum levels compared to healthy controls and patients with long-lasting disease. In conclusion, our data suggest the apoTf pivotal role in the perpetuation of type 1 diabetes pathology.
T2  - Clinical and Experimental Immunology
T1  - The immunobiology of apotransferrin in type 1 diabetes
IS  - 3
VL  - 169
SP  - 5
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1119
ER  - 

@article{
author = "Mangano, Katia and Fagone, Paolo and Di Mauro, M and Ascione, E and Maiello, V and Milicić, Tanja and Jotić, Aleksandra Z and Lalić, Nebojsa M and Saksida, Tamara and Stojanović, Ivana D. and Selmi, C and Farina, C and Stošić-Grujičić, Stanislava and Meroni, P and Nicoletti, Ferdinando",
year = "2012",
abstract = "The transferrin (Tf) family of iron binding proteins includes important endogenous modulators of the immune function that may modulate autoimmune diseases. To define more clearly the role of apotransferrin (apoTf) in type 1 diabetes we determined the impact of this protein on type 1 diabetes as investigated in islet cells, animal models and patient sera. First, we demonstrated that recombinant apoTf counteracts the cytokine-induced death of murine pancreatic islet cells. Secondly, human apoTf administration favourably influences the course of type 1 diabetes in animal models, resulting in protection against disease development that was associated with reduction of insulitis and reduced levels of proinflammatory cytokines. Finally, we confirmed that patients with newly diagnosed type 1 diabetes manifest significantly lower apoTf serum levels compared to healthy controls and patients with long-lasting disease. In conclusion, our data suggest the apoTf pivotal role in the perpetuation of type 1 diabetes pathology.",
journal = "Clinical and Experimental Immunology",
title = "The immunobiology of apotransferrin in type 1 diabetes",
number = "3",
volume = "169",
pages = "5-252",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1119"
}

Mangano, K., Fagone, P., Di Mauro, M., Ascione, E., Maiello, V., Milicić, T., Jotić, A. Z., Lalić, N. M., Saksida, T., Stojanović, I. D., Selmi, C., Farina, C., Stošić-Grujičić, S., Meroni, P.,& Nicoletti, F.. (2012). The immunobiology of apotransferrin in type 1 diabetes. in Clinical and Experimental Immunology, 169(3), 5-252.
https://hdl.handle.net/21.15107/rcub_ibiss_1119

Mangano K, Fagone P, Di Mauro M, Ascione E, Maiello V, Milicić T, Jotić AZ, Lalić NM, Saksida T, Stojanović ID, Selmi C, Farina C, Stošić-Grujičić S, Meroni P, Nicoletti F. The immunobiology of apotransferrin in type 1 diabetes. in Clinical and Experimental Immunology. 2012;169(3):5-252.
https://hdl.handle.net/21.15107/rcub_ibiss_1119 .

Mangano, Katia, Fagone, Paolo, Di Mauro, M, Ascione, E, Maiello, V, Milicić, Tanja, Jotić, Aleksandra Z, Lalić, Nebojsa M, Saksida, Tamara, Stojanović, Ivana D., Selmi, C, Farina, C, Stošić-Grujičić, Stanislava, Meroni, P, Nicoletti, Ferdinando, "The immunobiology of apotransferrin in type 1 diabetes" in Clinical and Experimental Immunology, 169, no. 3 (2012):5-252,
https://hdl.handle.net/21.15107/rcub_ibiss_1119 .