TY  - CONF
AU  - Hočevar, Katarina
AU  - Nolić, Vladimir
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6204
AB  - Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.
PB  - Beograd: University, Faculty of Biology
C3  - Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
T1  - Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6204
ER  - 

@conference{
author = "Hočevar, Katarina and Nolić, Vladimir and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2017",
abstract = "Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.",
publisher = "Beograd: University, Faculty of Biology",
journal = "Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia",
title = "Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6204"
}

Hočevar, K., Nolić, V., Budečević, S.,& Manitašević Jovanović, S.. (2017). Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
Beograd: University, Faculty of Biology., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_6204

Hočevar K, Nolić V, Budečević S, Manitašević Jovanović S. Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. 2017;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_6204 .

Hočevar, Katarina, Nolić, Vladimir, Budečević, Sanja, Manitašević Jovanović, Sanja, "Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties" in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia (2017):39,
https://hdl.handle.net/21.15107/rcub_ibiss_6204 .

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana B
AU  - Nolić, Vladimir
AU  - Banjac, Nevena
AU  - Ćalić-Dragosavac, Dušica D.
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1177
AB  - Hygromycin (hyg) at low doses (0.5-1.0 mg l(-1)) promoted somatic embryogenesis from apical sections of spinach lateral roots. The highest promoting effect on both the frequency of regeneration and the mean number of somatic embryos (SE) per explant was achieved at 0.5 mg l(-1) hyg. With increasing the concentration of hyg to 1 mg l(-1), the regeneration frequency decreased, while the mean SE number remained significantly higher than in control (hyg-free medium). Complete inhibition of SE regeneration started at 7.5 mg l(-1) hyg. Moreover, hyg efficiently promoted the process of secondary somatic embryogenesis. Compared to control, a 2.75-fold increase in the secondary somatic embryo (SSE) mean number was obtained at 0.5 mg l(-1) hyg, and the increment was still discernible at 1.0 and 2.5 mg l(-1) hyg. Both primary SE and SSE explants became completely necrotic at 12.5 mg l(-1) hyg. Since attempts with direct selection at 20 mg l(-1) hyg proved unsuccessful, the results obtained in this study suggest that a stepwise selection procedure is suitable, starting with selection at 0.5 mg l(-1) hyg, to exploit the promoting effect of low hyg doses on SE regeneration from transformed cells, then gradually increasing the hyg concentration to 20 mg l(-1) for final selection. Complete SE and SSE explant mortality at hyg above 12.5 mg l(-1) guarantees a low possibility of escape during the selection process. This study will be useful for increasing the efficiency of transgenic plant regeneration following genetic transformation in spinach.
T2  - Plant Cell Tissue and Organ Culture
T1  - Hygromycin promotes somatic embryogenesis in spinach
IS  - 3
VL  - 109
EP  - 579
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1177
ER  - 

@article{
author = "Milojević, Jelena and Tubić, Ljiljana B and Nolić, Vladimir and Banjac, Nevena and Ćalić-Dragosavac, Dušica D. and Vinterhalter, Branka and Zdravković-Korać, Snežana",
year = "2012",
abstract = "Hygromycin (hyg) at low doses (0.5-1.0 mg l(-1)) promoted somatic embryogenesis from apical sections of spinach lateral roots. The highest promoting effect on both the frequency of regeneration and the mean number of somatic embryos (SE) per explant was achieved at 0.5 mg l(-1) hyg. With increasing the concentration of hyg to 1 mg l(-1), the regeneration frequency decreased, while the mean SE number remained significantly higher than in control (hyg-free medium). Complete inhibition of SE regeneration started at 7.5 mg l(-1) hyg. Moreover, hyg efficiently promoted the process of secondary somatic embryogenesis. Compared to control, a 2.75-fold increase in the secondary somatic embryo (SSE) mean number was obtained at 0.5 mg l(-1) hyg, and the increment was still discernible at 1.0 and 2.5 mg l(-1) hyg. Both primary SE and SSE explants became completely necrotic at 12.5 mg l(-1) hyg. Since attempts with direct selection at 20 mg l(-1) hyg proved unsuccessful, the results obtained in this study suggest that a stepwise selection procedure is suitable, starting with selection at 0.5 mg l(-1) hyg, to exploit the promoting effect of low hyg doses on SE regeneration from transformed cells, then gradually increasing the hyg concentration to 20 mg l(-1) for final selection. Complete SE and SSE explant mortality at hyg above 12.5 mg l(-1) guarantees a low possibility of escape during the selection process. This study will be useful for increasing the efficiency of transgenic plant regeneration following genetic transformation in spinach.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Hygromycin promotes somatic embryogenesis in spinach",
number = "3",
volume = "109",
pages = "579",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1177"
}

Milojević, J., Tubić, L. B., Nolić, V., Banjac, N., Ćalić-Dragosavac, D. D., Vinterhalter, B.,& Zdravković-Korać, S.. (2012). Hygromycin promotes somatic embryogenesis in spinach. in Plant Cell Tissue and Organ Culture, 109(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1177

Milojević J, Tubić LB, Nolić V, Banjac N, Ćalić-Dragosavac DD, Vinterhalter B, Zdravković-Korać S. Hygromycin promotes somatic embryogenesis in spinach. in Plant Cell Tissue and Organ Culture. 2012;109(3):null-579.
https://hdl.handle.net/21.15107/rcub_ibiss_1177 .

Milojević, Jelena, Tubić, Ljiljana B, Nolić, Vladimir, Banjac, Nevena, Ćalić-Dragosavac, Dušica D., Vinterhalter, Branka, Zdravković-Korać, Snežana, "Hygromycin promotes somatic embryogenesis in spinach" in Plant Cell Tissue and Organ Culture, 109, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1177 .