@phdthesis{
author = "Slavić, Marija",
year = "2011",
abstract = "Fuleren C60 je alotropska modifikacija ugljenika i on, kao i njegovi derivati, već imaju
značajan uticaj na razvoj biomedicinske nanotehnologije. Potencijal nanočestica je veliki, a u
slučaju fulerena najveći je njegova primena kao finog biomarkera u molekularnoj dijagnostici.
Ispitivanja polihidroksilovanih derivata fulerena pokazali su da oni imaju širok spektar
bioloških efekata. Fulerenol C60(OH)24, vodorastvorni derivat fulerena C60, privlači veliku
pažnju istraživača. Najviše je ispitivana njegova sposobnost fotosenzitivnosti, kao i
sposobnost uklanjanja slobodnih radikala (free radical “sponge” ili free radical scavenger).
Takođe, u žiži interesovanja su i anitproliferativna i antikancerogena dejstva ove supstance.
Zbog specifične strukture koja podseća na fudbalsku loptu, kandidat je za ulogu nosača
supstanci i lekova. Ovakva primena lekova omogućila bi njihovo specifično delovanje na
ciljne ćelije ili tkivo. Pokazano je, takođe, da fulerenol ima sposobnost interakcije sa dsDNK,
što je veoma značajno sa aspekta razumevanja penetracije ove nanočestice kroz membranu
ćelije i njegove intraćelijske aktivnosti.
Potencijalna široka primena fulerenola u biomedicini zahteva i obimna farmakološka i
toksikološka ispitivanja. Do sada ima malo rezultata farmakoloških ispitivanja fulerenola a
direktni farmakološki efekti dobijeni aplikacijom fulerenola na izolovane organe koji
poseduju glatku muskulaturu su sporadično ispitivani.
S druge strane, supstanca koja se već dugo istražuje ali je, po nekima, i dalje nedovoljno
prisutna u farmakologiji je dimetil sulfoksid (DMSO). Osim što je odličan rastvarač, topikalni
analgetik i nosač supstanci, DMSO-u se pripisuje sposobnost uklanjanja slobodnih radikala i
smatra se da ima pozitivne efekte na oštećenja kardiovaskularnog sistema, centralnog nervnog
sistema, bubrežna oboljenja, defekte u strukturi vezivnog tkiva itd.
Promene koje fulerenol i DMSO mogu izazvati na kontraktilnost glatke muskulature su
izuzetno važne za mogućnost njihove šire primene u medicini i farmakologiji.
Ileum je kao deo digestivnog trakta, veoma značajan model sistem za izučavanje dejstva
supstance koja bi se potencijalno mogla koristiti u farmakologiji.
Uterus pokazuje promene tokom mesečnog ciklusa, kako u kontraktilnosti, tako i u sastavu
mikroflore i aktivnosti enzima antioksidativne zaštite.
Ispitivane supstance mogu uticati na promene parametara kao što su: frekvenca,
amplituda, trajanje i smer propagacije kontrakcija., Fullerene (C60) is the carbon allotrope and as its derivatives already has a significant
impact on biomedical nanotechnology. The potential of nanoparticles is large but one of the
most relevant biological features of fullerene is its application as a biomarker in molecular
diagnostics. Polyhidroxylated fullerene derivatives have been shown to display a wide range
of biological effects. Fullerenol C60(OH)24, as a soluble derivative of fullerene C60, attracts the
attention of a number of researchers. The most studied features of fullerenol are
photosensitization and the ability to quench various free radicals, behaving as a free radical
“sponge” or free radical scavenger. Anitproliferative and anti-cancer properties of this
substance are also in the spotlight. Due to its specific structure that resembles a soccer ball,
fullerenol is a candidate for a drug carrier. This could enable specific action on target cells or
tissue. Ability of fullerenol to interact with dsDNK has also been demonstrated, which is very
important in understanding the penetration of nanoparticles through the cell membrane and
their intracellular activities.
Potentially wide application of fullerenol in biomedicine requires extensive
pharmacological and toxicological investigations. Pharmacological tests and direct
pharmacological effects obtained from application of fullerenol on isolated organs that
possess smooth musculature were investigated only sporadically.
On the other hand, dimethyl sulfoxide (DMSO) has long been investigated but is,
according to some, still insufficiently represented in the pharmacology. Besides being an
excellent solvent, topical analgetic and drug carrier, DMSO has the ability to remove free
radicals and is considered to have positive effects on the damage of cardiovascular system,
central nervous system, renal disease, defects in the structure of connective tissue etc.
Both fullerenol and DMSO can cause changes in the contractility of smooth muscles,
which is extremely important for their potentially wider application in pharmacology.
Ileum, as a part of the digestive tract is an important model system for the investigation of
a substance that could potentially be used in pharmacology. Uterus contractility, microflora and the activity of antioxidative enzymes change during menstrual cycle. Investigated
substances can change parameters of contraction, such as: frequency, amplitude, duration and
direction.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Direktni farmakološki efekti fulerenola C60(OH)24 na modelu izolovanog glatkog mišića pacova i uloga antioksidativnog sistema, Direct pharmacological effects of fullerenol С60(ОН)24 on an isolated
rat smooth muscle model and the role of the antioxidant system",
pages = "1-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4135"
}