TY  - JOUR
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Mojić, Marija
AU  - Malaponte, Graziella
AU  - Libra, Massimo
AU  - Cardile, Vera
AU  - Miljković, Đorđe
AU  - Harhaji-Trajković, Ljubica
AU  - Dabideen, Darrin
AU  - Cheng, Kai Fan
AU  - Bevelacqua, Ylenia
AU  - Donia, Marco
AU  - Garotta, Gianni
AU  - Ai-Abed, Yousef
AU  - Stošić-Grujičić, Stanislava
AU  - Nicoletti, Ferdinando
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1517
AB  - In this study we evaluated the effects of the new NO donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) on the A375 human melanoma cell line. Treatment with the drug led to concentration-dependent reduction of mitochondrial respiration and number of viable cells in cultures. Decreased cell viability correlated with release and internalization of NO and was neutralized by the extracellular scavenger hemoglobin. GIT-27NO neither influenced cell division nor induced accidental or autophagic cell death. Early signs of apoptosis were observed upon coculture with the drug, and resulting in marked accumulation of hypodiploid cells, suggesting that the induction of apoptosis is one primary mode of action of the compound in A375 cells. GIT-27NO significantly inhibited the expression of the transcription repressor and apoptotic resistant factor YY1 and, in parallel, augmented the presence of total p53. The capacity of GIT-27NO to induce p53-mediated apoptosis along with inhibition of YY1 repressor in A375 melanoma cells indicates that GIT-27NO possesses an important anti-cancer pharmacological profile. The findings suggest the potential therapeutic use of GIT-27NO in the clinical setting. (C) 2008 Elsevier Inc. All rights reserved.
T2  - Nitric Oxide-Biology and Chemistry
T1  - Novel nitric oxide-donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) induces p53 mediated apoptosis in human A375 melanoma cells
IS  - 2
VL  - 19
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1517
ER  - 

@article{
author = "Mijatović, Sanja and Maksimović-Ivanić, Danijela and Mojić, Marija and Malaponte, Graziella and Libra, Massimo and Cardile, Vera and Miljković, Đorđe and Harhaji-Trajković, Ljubica and Dabideen, Darrin and Cheng, Kai Fan and Bevelacqua, Ylenia and Donia, Marco and Garotta, Gianni and Ai-Abed, Yousef and Stošić-Grujičić, Stanislava and Nicoletti, Ferdinando",
year = "2008",
abstract = "In this study we evaluated the effects of the new NO donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) on the A375 human melanoma cell line. Treatment with the drug led to concentration-dependent reduction of mitochondrial respiration and number of viable cells in cultures. Decreased cell viability correlated with release and internalization of NO and was neutralized by the extracellular scavenger hemoglobin. GIT-27NO neither influenced cell division nor induced accidental or autophagic cell death. Early signs of apoptosis were observed upon coculture with the drug, and resulting in marked accumulation of hypodiploid cells, suggesting that the induction of apoptosis is one primary mode of action of the compound in A375 cells. GIT-27NO significantly inhibited the expression of the transcription repressor and apoptotic resistant factor YY1 and, in parallel, augmented the presence of total p53. The capacity of GIT-27NO to induce p53-mediated apoptosis along with inhibition of YY1 repressor in A375 melanoma cells indicates that GIT-27NO possesses an important anti-cancer pharmacological profile. The findings suggest the potential therapeutic use of GIT-27NO in the clinical setting. (C) 2008 Elsevier Inc. All rights reserved.",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Novel nitric oxide-donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) induces p53 mediated apoptosis in human A375 melanoma cells",
number = "2",
volume = "19",
pages = "183",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1517"
}

Mijatović, S., Maksimović-Ivanić, D., Mojić, M., Malaponte, G., Libra, M., Cardile, V., Miljković, Đ., Harhaji-Trajković, L., Dabideen, D., Cheng, K. F., Bevelacqua, Y., Donia, M., Garotta, G., Ai-Abed, Y., Stošić-Grujičić, S.,& Nicoletti, F.. (2008). Novel nitric oxide-donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) induces p53 mediated apoptosis in human A375 melanoma cells. in Nitric Oxide-Biology and Chemistry, 19(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1517

Mijatović S, Maksimović-Ivanić D, Mojić M, Malaponte G, Libra M, Cardile V, Miljković Đ, Harhaji-Trajković L, Dabideen D, Cheng KF, Bevelacqua Y, Donia M, Garotta G, Ai-Abed Y, Stošić-Grujičić S, Nicoletti F. Novel nitric oxide-donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) induces p53 mediated apoptosis in human A375 melanoma cells. in Nitric Oxide-Biology and Chemistry. 2008;19(2):null-183.
https://hdl.handle.net/21.15107/rcub_ibiss_1517 .

Mijatović, Sanja, Maksimović-Ivanić, Danijela, Mojić, Marija, Malaponte, Graziella, Libra, Massimo, Cardile, Vera, Miljković, Đorđe, Harhaji-Trajković, Ljubica, Dabideen, Darrin, Cheng, Kai Fan, Bevelacqua, Ylenia, Donia, Marco, Garotta, Gianni, Ai-Abed, Yousef, Stošić-Grujičić, Stanislava, Nicoletti, Ferdinando, "Novel nitric oxide-donating compound (S,R)-3-phenyl-4,5-dihydro-5-isoxazole acetic acid-nitric oxide (GIT-27NO) induces p53 mediated apoptosis in human A375 melanoma cells" in Nitric Oxide-Biology and Chemistry, 19, no. 2 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1517 .