TY  - JOUR
AU  - Stanojević, Maja
AU  - Li, Kun
AU  - Stamenković, Gorana
AU  - Ilić, Bojan
AU  - Paunović, Milan
AU  - Pešić, Branislav
AU  - Đurić Maslovara, Ivana
AU  - Šiljić, Marina
AU  - Ćirković, Valentina
AU  - Zhang, Yongzhen
PY  - 2020
UR  - https://www.mdpi.com/1999-4915/12/9/975
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32887342
UR  - https://radar.ibiss.bg.ac.rs/123456789/3880
AB  - Hematophagous arthropods are important vectors for zoonotic pathogens. To date, a huge number of viruses have been identified in these arthropods, with a considerable proportion of them being human pathogens. However, the viromes of hematophagous arthropods are still largely unresearched. In this study, a number of arthropods were collected from Belgrade, Serbia including mosquitoes, ticks and bedbugs. The viromes of these arthropods were identified and characterized using Illumina MiSeq sequencing. In total, 21 viruses belonging to 11 families were characterized, with 11 of them representing novel species. These results may contribute to our knowledge of RNA viruses in arthropods and the discovery of novel human pathogens.
PB  - NLM (Medline)
T2  - Viruses
T1  - Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia
IS  - 9
VL  - 12
DO  - 10.3390/v12090975
SP  - 975
ER  - 

@article{
author = "Stanojević, Maja and Li, Kun and Stamenković, Gorana and Ilić, Bojan and Paunović, Milan and Pešić, Branislav and Đurić Maslovara, Ivana and Šiljić, Marina and Ćirković, Valentina and Zhang, Yongzhen",
year = "2020",
abstract = "Hematophagous arthropods are important vectors for zoonotic pathogens. To date, a huge number of viruses have been identified in these arthropods, with a considerable proportion of them being human pathogens. However, the viromes of hematophagous arthropods are still largely unresearched. In this study, a number of arthropods were collected from Belgrade, Serbia including mosquitoes, ticks and bedbugs. The viromes of these arthropods were identified and characterized using Illumina MiSeq sequencing. In total, 21 viruses belonging to 11 families were characterized, with 11 of them representing novel species. These results may contribute to our knowledge of RNA viruses in arthropods and the discovery of novel human pathogens.",
publisher = "NLM (Medline)",
journal = "Viruses",
title = "Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia",
number = "9",
volume = "12",
doi = "10.3390/v12090975",
pages = "975"
}

Stanojević, M., Li, K., Stamenković, G., Ilić, B., Paunović, M., Pešić, B., Đurić Maslovara, I., Šiljić, M., Ćirković, V.,& Zhang, Y.. (2020). Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia. in Viruses
NLM (Medline)., 12(9), 975.
https://doi.org/10.3390/v12090975

Stanojević M, Li K, Stamenković G, Ilić B, Paunović M, Pešić B, Đurić Maslovara I, Šiljić M, Ćirković V, Zhang Y. Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia. in Viruses. 2020;12(9):975.
doi:10.3390/v12090975 .

Stanojević, Maja, Li, Kun, Stamenković, Gorana, Ilić, Bojan, Paunović, Milan, Pešić, Branislav, Đurić Maslovara, Ivana, Šiljić, Marina, Ćirković, Valentina, Zhang, Yongzhen, "Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia" in Viruses, 12, no. 9 (2020):975,
https://doi.org/10.3390/v12090975 . .

TY  - JOUR
AU  - Stanojević, Maja
AU  - Ćirković, Valentina
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stamenković, Gorana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S187603411930108X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3316
AB  - BACKGROUND Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. METHODS Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral antibodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. RESULTS PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. CONCLUSIONS Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation.
T2  - Journal of Infection and Public Health
T1  - Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.
DO  - 10.1016/j.jiph.2019.02.021
ER  - 

@article{
author = "Stanojević, Maja and Ćirković, Valentina and Siljić, Marina and Gligić, Ana and Stamenković, Gorana",
year = "2019",
abstract = "BACKGROUND Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. METHODS Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral antibodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. RESULTS PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. CONCLUSIONS Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation.",
journal = "Journal of Infection and Public Health",
title = "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.",
doi = "10.1016/j.jiph.2019.02.021"
}

Stanojević, M., Ćirković, V., Siljić, M., Gligić, A.,& Stamenković, G.. (2019). Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.. in Journal of Infection and Public Health.
https://doi.org/10.1016/j.jiph.2019.02.021

Stanojević M, Ćirković V, Siljić M, Gligić A, Stamenković G. Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.. in Journal of Infection and Public Health. 2019;.
doi:10.1016/j.jiph.2019.02.021 .

Stanojević, Maja, Ćirković, Valentina, Siljić, Marina, Gligić, Ana, Stamenković, Gorana, "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011." in Journal of Infection and Public Health (2019),
https://doi.org/10.1016/j.jiph.2019.02.021 . .

TY  - JOUR
AU  - Ćirković, Valentina
AU  - Stamenković, Gorana
AU  - Jovanović, Jelena
AU  - Šiljić, Marina
AU  - Paunović, Milan
AU  - Stanojević, Maja
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2547
AB  - Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.
T2  - Tropical Biomedicine
T1  - Failure to detect viral RNA in bat samples collected in the Balkan region
IS  - 4
VL  - 33
SP  - 780
EP  - 785
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2547
ER  - 

@article{
author = "Ćirković, Valentina and Stamenković, Gorana and Jovanović, Jelena and Šiljić, Marina and Paunović, Milan and Stanojević, Maja",
year = "2016",
abstract = "Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.",
journal = "Tropical Biomedicine",
title = "Failure to detect viral RNA in bat samples collected in the Balkan region",
number = "4",
volume = "33",
pages = "780-785",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2547"
}

Ćirković, V., Stamenković, G., Jovanović, J., Šiljić, M., Paunović, M.,& Stanojević, M.. (2016). Failure to detect viral RNA in bat samples collected in the Balkan region. in Tropical Biomedicine, 33(4), 780-785.
https://hdl.handle.net/21.15107/rcub_ibiss_2547

Ćirković V, Stamenković G, Jovanović J, Šiljić M, Paunović M, Stanojević M. Failure to detect viral RNA in bat samples collected in the Balkan region. in Tropical Biomedicine. 2016;33(4):780-785.
https://hdl.handle.net/21.15107/rcub_ibiss_2547 .

Ćirković, Valentina, Stamenković, Gorana, Jovanović, Jelena, Šiljić, Marina, Paunović, Milan, Stanojević, Maja, "Failure to detect viral RNA in bat samples collected in the Balkan region" in Tropical Biomedicine, 33, no. 4 (2016):780-785,
https://hdl.handle.net/21.15107/rcub_ibiss_2547 .