TY  - CONF
AU  - Martinović, Tamara
AU  - Ćirić, Darko
AU  - Pantić, Igor
AU  - Lalić, Katarina
AU  - Rasulić, Iva
AU  - Despotović, Sanja
AU  - Lalić, Ivana
AU  - Đuričić, Danica
AU  - Vučićević, Ljubica
AU  - Misirkić Marjanović, Maja
AU  - Trajković, Vladimir
AU  - Bumbaširević, Vladimir
AU  - Kravić-Stevović, Tamara
PY  - 2019
UR  - https://klinbiolabmed.rs/wp-content/uploads/2022/05/Knjiga-sazetka-Kongresa.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6662
AB  - Uvod: Osobine tip 2 dijabetes melitusa (T2DM) su insulinska rezistencija, poremećena sekrecija insulina i hiperglikemija. Kao početna terapija T2DM koristi se metformin. Novija istraživanja su pokazala da u T2DM dolazi do morfoloških promena u izgledu nukleusa u vidu nepravilnosti oblika i binukelacije nukleusa.
Cilj: Cilj istraživanja je da se analiziraju ultrastrukturne karakteristike nukleusa limfocita periferne krvi kod pacijenata sa T2DM pomoću kompjuterizovane analize slike, fraktalne i teksturalne analize, kao i utvrđivanje efekta metformina na karakteristike nukleusa.
Metodologija: Mononuklearne ćelije izolovane iz periferne krvi novootkrivenih T2DM bolesnika, bolesnika lečenih metforminom i zdravih ispitanika analizirane su na transmisionom elektronskom mikroskopu (TEM). Primenom ImageJ programa analizirani su oblik i procenat heterohromatina, kao i fraktalna i teksturalna analiza nukleusa limfocita.
Rezultati: Limfociti zdravih osoba su imali okrugle, predominantno heterohromatične nukleuse i malu količinu citoplazme sa retko prisutnim organelama, dok su limfociti T2DM bolesnika imali euhromatične nukleu uz povećanje strukturnih praznina kod T2DM bolesnika. Nivo glukoze našte i HbA1c koreliraju sa fraktalnom dimenzijom i sa parametrima oblika nukleusa. Postoji korelacija između nivoa triglicerida u krvi i fraktalne dimenzije nukleusa.
Zaključak: Nukleusi limfocita bolesnika sa T2DM su nepravilnog oblika i sa većom količinom euhromatina, a promena njihovog izgleda je u vezi sa nivoom glikemije.
PB  - Beograd: Srpsko lekarsko društvo, Sekcija kliničke biohemije
C3  - Knjiga sažetaka: 1. kongres kliničkih biohemičara i specijalista laboratorijske medicine Srbije sa međunarodnim učešćem;  2019 Nov 27-29; Belgrade, Serbia
T1  - Uticaj glikemije i lipidnog statusa na morfološke karakteristike nukleusa kod pacijenata sa tip 2 dijabetes melitusom
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6662
ER  - 

@conference{
author = "Martinović, Tamara and Ćirić, Darko and Pantić, Igor and Lalić, Katarina and Rasulić, Iva and Despotović, Sanja and Lalić, Ivana and Đuričić, Danica and Vučićević, Ljubica and Misirkić Marjanović, Maja and Trajković, Vladimir and Bumbaširević, Vladimir and Kravić-Stevović, Tamara",
year = "2019",
abstract = "Uvod: Osobine tip 2 dijabetes melitusa (T2DM) su insulinska rezistencija, poremećena sekrecija insulina i hiperglikemija. Kao početna terapija T2DM koristi se metformin. Novija istraživanja su pokazala da u T2DM dolazi do morfoloških promena u izgledu nukleusa u vidu nepravilnosti oblika i binukelacije nukleusa.
Cilj: Cilj istraživanja je da se analiziraju ultrastrukturne karakteristike nukleusa limfocita periferne krvi kod pacijenata sa T2DM pomoću kompjuterizovane analize slike, fraktalne i teksturalne analize, kao i utvrđivanje efekta metformina na karakteristike nukleusa.
Metodologija: Mononuklearne ćelije izolovane iz periferne krvi novootkrivenih T2DM bolesnika, bolesnika lečenih metforminom i zdravih ispitanika analizirane su na transmisionom elektronskom mikroskopu (TEM). Primenom ImageJ programa analizirani su oblik i procenat heterohromatina, kao i fraktalna i teksturalna analiza nukleusa limfocita.
Rezultati: Limfociti zdravih osoba su imali okrugle, predominantno heterohromatične nukleuse i malu količinu citoplazme sa retko prisutnim organelama, dok su limfociti T2DM bolesnika imali euhromatične nukleu uz povećanje strukturnih praznina kod T2DM bolesnika. Nivo glukoze našte i HbA1c koreliraju sa fraktalnom dimenzijom i sa parametrima oblika nukleusa. Postoji korelacija između nivoa triglicerida u krvi i fraktalne dimenzije nukleusa.
Zaključak: Nukleusi limfocita bolesnika sa T2DM su nepravilnog oblika i sa većom količinom euhromatina, a promena njihovog izgleda je u vezi sa nivoom glikemije.",
publisher = "Beograd: Srpsko lekarsko društvo, Sekcija kliničke biohemije",
journal = "Knjiga sažetaka: 1. kongres kliničkih biohemičara i specijalista laboratorijske medicine Srbije sa međunarodnim učešćem;  2019 Nov 27-29; Belgrade, Serbia",
title = "Uticaj glikemije i lipidnog statusa na morfološke karakteristike nukleusa kod pacijenata sa tip 2 dijabetes melitusom",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6662"
}

Martinović, T., Ćirić, D., Pantić, I., Lalić, K., Rasulić, I., Despotović, S., Lalić, I., Đuričić, D., Vučićević, L., Misirkić Marjanović, M., Trajković, V., Bumbaširević, V.,& Kravić-Stevović, T.. (2019). Uticaj glikemije i lipidnog statusa na morfološke karakteristike nukleusa kod pacijenata sa tip 2 dijabetes melitusom. in Knjiga sažetaka: 1. kongres kliničkih biohemičara i specijalista laboratorijske medicine Srbije sa međunarodnim učešćem;  2019 Nov 27-29; Belgrade, Serbia
Beograd: Srpsko lekarsko društvo, Sekcija kliničke biohemije., 58.
https://hdl.handle.net/21.15107/rcub_ibiss_6662

Martinović T, Ćirić D, Pantić I, Lalić K, Rasulić I, Despotović S, Lalić I, Đuričić D, Vučićević L, Misirkić Marjanović M, Trajković V, Bumbaširević V, Kravić-Stevović T. Uticaj glikemije i lipidnog statusa na morfološke karakteristike nukleusa kod pacijenata sa tip 2 dijabetes melitusom. in Knjiga sažetaka: 1. kongres kliničkih biohemičara i specijalista laboratorijske medicine Srbije sa međunarodnim učešćem;  2019 Nov 27-29; Belgrade, Serbia. 2019;:58.
https://hdl.handle.net/21.15107/rcub_ibiss_6662 .

Martinović, Tamara, Ćirić, Darko, Pantić, Igor, Lalić, Katarina, Rasulić, Iva, Despotović, Sanja, Lalić, Ivana, Đuričić, Danica, Vučićević, Ljubica, Misirkić Marjanović, Maja, Trajković, Vladimir, Bumbaširević, Vladimir, Kravić-Stevović, Tamara, "Uticaj glikemije i lipidnog statusa na morfološke karakteristike nukleusa kod pacijenata sa tip 2 dijabetes melitusom" in Knjiga sažetaka: 1. kongres kliničkih biohemičara i specijalista laboratorijske medicine Srbije sa međunarodnim učešćem;  2019 Nov 27-29; Belgrade, Serbia (2019):58,
https://hdl.handle.net/21.15107/rcub_ibiss_6662 .

TY  - JOUR
AU  - Vujičić, Milica
AU  - Saksida, Tamara
AU  - Despotović, Sanja
AU  - Soković Bajić, Svetlana
AU  - Lalić, Ivana
AU  - Koprivica, Ivan
AU  - Mićanović, Dragica
AU  - Golić, Natasa
AU  - Tolinački, Maja
AU  - Stojanović, Ivana D.
PY  - 2018
UR  - http://www.nature.com/articles/s41598-018-24706-3
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5910418
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3049
AB  - Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-γ in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.
T2  - Scientific Reports
T1  - The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.
IS  - 1
VL  - 8
DO  - 10.1038/s41598-018-24706-3
SP  - 6337
ER  - 

@article{
author = "Vujičić, Milica and Saksida, Tamara and Despotović, Sanja and Soković Bajić, Svetlana and Lalić, Ivana and Koprivica, Ivan and Mićanović, Dragica and Golić, Natasa and Tolinački, Maja and Stojanović, Ivana D.",
year = "2018",
abstract = "Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-γ in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.",
journal = "Scientific Reports",
title = "The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.",
number = "1",
volume = "8",
doi = "10.1038/s41598-018-24706-3",
pages = "6337"
}

Vujičić, M., Saksida, T., Despotović, S., Soković Bajić, S., Lalić, I., Koprivica, I., Mićanović, D., Golić, N., Tolinački, M.,& Stojanović, I. D.. (2018). The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.. in Scientific Reports, 8(1), 6337.
https://doi.org/10.1038/s41598-018-24706-3

Vujičić M, Saksida T, Despotović S, Soković Bajić S, Lalić I, Koprivica I, Mićanović D, Golić N, Tolinački M, Stojanović ID. The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.. in Scientific Reports. 2018;8(1):6337.
doi:10.1038/s41598-018-24706-3 .

Vujičić, Milica, Saksida, Tamara, Despotović, Sanja, Soković Bajić, Svetlana, Lalić, Ivana, Koprivica, Ivan, Mićanović, Dragica, Golić, Natasa, Tolinački, Maja, Stojanović, Ivana D., "The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier." in Scientific Reports, 8, no. 1 (2018):6337,
https://doi.org/10.1038/s41598-018-24706-3 . .

TY  - JOUR
AU  - Lalić, Ivana M.
AU  - Bichele, Rudolf
AU  - Repar, Anja
AU  - Despotović, Sanja Z.
AU  - Petričević, Saša
AU  - Laan, Martti
AU  - Peterson, Pärt
AU  - Westermann, Jürgen
AU  - Milićević, Živana
AU  - Mirkov, Ivana
AU  - Milićević, Novica M.
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0940960217301553?via%3Dihub
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29289711
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3020
AB  - It is well known that bacterial lipopolysaccharide (LPS) induces migration of several cellular populations within the spleen. However, there are no data about the impact of LPS on B and T lymphocytes present in the red pulp. Therefore, we used an experimental model in which we tested the effects of intravenously injected LPS on the molecular, cellular and structural changes of the spleen, with special reference to the red pulp lymphocytes. We discovered that LPS induced a massive relocation of B and T lymphocytes from the splenic red pulp, which was independent of the tumor necrosis factor receptor-1 signaling axis. Early after LPS treatment, quantitative real-time PCR analysis revealed the elevated levels of mRNA encoding numerous chemokines and proinflammatory cytokines (XCL1, CXCL9, CXCL10, CCL3, CCL4, CCL5, CCL17, CCL20, CCL22, TNFα and LTα) which affect the navigation and activities of B and T lymphocytes in the lymphoid tissues. An extreme increase in mRNA levels for CCL20 was detected in the white pulp of the LPS-treated mice. The CCL20-expressing cells were localized in the PALS. Some smaller CCL20-expressing cells were evenly dispersed in the B cell zone. Thus, our study provides new knowledge of how microbial products could be involved in shaping the structure of lymphatic organs.
T2  - Annals of anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
T1  - Lipopolysaccharide induces tumor necrosis factor receptor-1 independent relocation of lymphocytes from the red pulp of the mouse spleen
VL  - 216
DO  - 10.1016/j.aanat.2017.12.002
SP  - 125
EP  - 134
ER  - 

@article{
author = "Lalić, Ivana M. and Bichele, Rudolf and Repar, Anja and Despotović, Sanja Z. and Petričević, Saša and Laan, Martti and Peterson, Pärt and Westermann, Jürgen and Milićević, Živana and Mirkov, Ivana and Milićević, Novica M.",
year = "2018",
abstract = "It is well known that bacterial lipopolysaccharide (LPS) induces migration of several cellular populations within the spleen. However, there are no data about the impact of LPS on B and T lymphocytes present in the red pulp. Therefore, we used an experimental model in which we tested the effects of intravenously injected LPS on the molecular, cellular and structural changes of the spleen, with special reference to the red pulp lymphocytes. We discovered that LPS induced a massive relocation of B and T lymphocytes from the splenic red pulp, which was independent of the tumor necrosis factor receptor-1 signaling axis. Early after LPS treatment, quantitative real-time PCR analysis revealed the elevated levels of mRNA encoding numerous chemokines and proinflammatory cytokines (XCL1, CXCL9, CXCL10, CCL3, CCL4, CCL5, CCL17, CCL20, CCL22, TNFα and LTα) which affect the navigation and activities of B and T lymphocytes in the lymphoid tissues. An extreme increase in mRNA levels for CCL20 was detected in the white pulp of the LPS-treated mice. The CCL20-expressing cells were localized in the PALS. Some smaller CCL20-expressing cells were evenly dispersed in the B cell zone. Thus, our study provides new knowledge of how microbial products could be involved in shaping the structure of lymphatic organs.",
journal = "Annals of anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft",
title = "Lipopolysaccharide induces tumor necrosis factor receptor-1 independent relocation of lymphocytes from the red pulp of the mouse spleen",
volume = "216",
doi = "10.1016/j.aanat.2017.12.002",
pages = "125-134"
}

Lalić, I. M., Bichele, R., Repar, A., Despotović, S. Z., Petričević, S., Laan, M., Peterson, P., Westermann, J., Milićević, Ž., Mirkov, I.,& Milićević, N. M.. (2018). Lipopolysaccharide induces tumor necrosis factor receptor-1 independent relocation of lymphocytes from the red pulp of the mouse spleen. in Annals of anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft, 216, 125-134.
https://doi.org/10.1016/j.aanat.2017.12.002

Lalić IM, Bichele R, Repar A, Despotović SZ, Petričević S, Laan M, Peterson P, Westermann J, Milićević Ž, Mirkov I, Milićević NM. Lipopolysaccharide induces tumor necrosis factor receptor-1 independent relocation of lymphocytes from the red pulp of the mouse spleen. in Annals of anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft. 2018;216:125-134.
doi:10.1016/j.aanat.2017.12.002 .

Lalić, Ivana M., Bichele, Rudolf, Repar, Anja, Despotović, Sanja Z., Petričević, Saša, Laan, Martti, Peterson, Pärt, Westermann, Jürgen, Milićević, Živana, Mirkov, Ivana, Milićević, Novica M., "Lipopolysaccharide induces tumor necrosis factor receptor-1 independent relocation of lymphocytes from the red pulp of the mouse spleen" in Annals of anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft, 216 (2018):125-134,
https://doi.org/10.1016/j.aanat.2017.12.002 . .