TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5690
AB  - Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11
DO  - 10.1097/MCG.0000000000001292
SP  - 103
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2019",
abstract = "Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11",
doi = "10.1097/MCG.0000000000001292",
pages = "103"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2019). The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 103.
https://doi.org/10.1097/MCG.0000000000001292

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;:103.
doi:10.1097/MCG.0000000000001292 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy (2019):103,
https://doi.org/10.1097/MCG.0000000000001292 . .