TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Lütjohann, Dieter
AU  - Renko, Kostja
AU  - Filipović, Branko
AU  - Radulović, Niko
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Nestorović, Nataša
AU  - Živanović, Jasmina
AU  - Manojlović-Stojanoski, Milica
AU  - Kӧhrle, Josef
AU  - Milošević, Verica
PY  - 2019
UR  - internal-pdf://Šošić-Jurjević et al. - 2019 - The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Chol.pdf
UR  - https://www.sciencedirect.com/science/article/pii/S0960076018307507
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3290
AB  - We examined whether isoflavones interfere with thyroid homeostasis, increase hepatic thyroid hormone concentrations and affect cholesterol metabolism in middle-aged (MA) male rats. Thirteen-month-old Wistar rats were injected subcutaneously with 35 mg/kg b.w./day of genistein, daidzein or vehicle (controls) for four weeks. Hepatic Dio1 gene expression was up-regulated by 70% (p < 0.001 for both) and Dio1 enzyme activity increased by 64% after genistein (p < 0.001) and 73% after daidzein treatment (p < 0.0001). Hepatic T3 was 75% higher (p < 0.05 for both), while T4 increased only after genistein treatment. Serum T4 concentrations were 31% lower in genistein- and 49% lower in dadzein-treated rats (p < 0.001 for both) compared with controls. Hepatic Cyp7a1 gene expression was up-regulated by 40% after genistein and 32% after daidzein treatment (p < 0.05 for both), in agreement with a 7α-hydroxycholesterol increase of 50% (p < 0.01) and 88% (p < 0.001), respectively. Serum 24- and 27-hydroxycholesterol were 30% lower (p < 0.05 for both), while only 24-hydroxycholesterol was decreased in the liver by 45% after genistein (p < 0.05) and 39% (p < 0.01) after dadzein treatment. Serum concentration of the cholesterol precursor desmosterol was 32% (p < 0.05) lower only after dadzein treatment alone, while both isoflavones elevated this parameter in the liver by 45% (p < 0.01). In conclusion, isoflavones increased T3 availability in the liver of MA males, despite decreasing serum T4. Hepatic increase of T3 possibly contributes to activation of the neutral pathway of cholesterol degradation into bile acids in the liver. While isoflavones obviously have the potential to trigger multiple mechanisms involved in cholesterol metabolism and oxysterol production, they failed to induce any hypocholesterolemic effect.
T2  - The Journal of Steroid Biochemistry and Molecular Biology
T1  - The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats.
VL  - 190
DO  - 10.1016/j.jsbmb.2019.03.009
SP  - 1
EP  - 10
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Lütjohann, Dieter and Renko, Kostja and Filipović, Branko and Radulović, Niko and Ajdžanović, Vladimir and Trifunović, Svetlana and Nestorović, Nataša and Živanović, Jasmina and Manojlović-Stojanoski, Milica and Kӧhrle, Josef and Milošević, Verica",
year = "2019",
abstract = "We examined whether isoflavones interfere with thyroid homeostasis, increase hepatic thyroid hormone concentrations and affect cholesterol metabolism in middle-aged (MA) male rats. Thirteen-month-old Wistar rats were injected subcutaneously with 35 mg/kg b.w./day of genistein, daidzein or vehicle (controls) for four weeks. Hepatic Dio1 gene expression was up-regulated by 70% (p < 0.001 for both) and Dio1 enzyme activity increased by 64% after genistein (p < 0.001) and 73% after daidzein treatment (p < 0.0001). Hepatic T3 was 75% higher (p < 0.05 for both), while T4 increased only after genistein treatment. Serum T4 concentrations were 31% lower in genistein- and 49% lower in dadzein-treated rats (p < 0.001 for both) compared with controls. Hepatic Cyp7a1 gene expression was up-regulated by 40% after genistein and 32% after daidzein treatment (p < 0.05 for both), in agreement with a 7α-hydroxycholesterol increase of 50% (p < 0.01) and 88% (p < 0.001), respectively. Serum 24- and 27-hydroxycholesterol were 30% lower (p < 0.05 for both), while only 24-hydroxycholesterol was decreased in the liver by 45% after genistein (p < 0.05) and 39% (p < 0.01) after dadzein treatment. Serum concentration of the cholesterol precursor desmosterol was 32% (p < 0.05) lower only after dadzein treatment alone, while both isoflavones elevated this parameter in the liver by 45% (p < 0.01). In conclusion, isoflavones increased T3 availability in the liver of MA males, despite decreasing serum T4. Hepatic increase of T3 possibly contributes to activation of the neutral pathway of cholesterol degradation into bile acids in the liver. While isoflavones obviously have the potential to trigger multiple mechanisms involved in cholesterol metabolism and oxysterol production, they failed to induce any hypocholesterolemic effect.",
journal = "The Journal of Steroid Biochemistry and Molecular Biology",
title = "The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats.",
volume = "190",
doi = "10.1016/j.jsbmb.2019.03.009",
pages = "1-10"
}

Šošić-Jurjević, B., Lütjohann, D., Renko, K., Filipović, B., Radulović, N., Ajdžanović, V., Trifunović, S., Nestorović, N., Živanović, J., Manojlović-Stojanoski, M., Kӧhrle, J.,& Milošević, V.. (2019). The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats.. in The Journal of Steroid Biochemistry and Molecular Biology, 190, 1-10.
https://doi.org/10.1016/j.jsbmb.2019.03.009

Šošić-Jurjević B, Lütjohann D, Renko K, Filipović B, Radulović N, Ajdžanović V, Trifunović S, Nestorović N, Živanović J, Manojlović-Stojanoski M, Kӧhrle J, Milošević V. The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats.. in The Journal of Steroid Biochemistry and Molecular Biology. 2019;190:1-10.
doi:10.1016/j.jsbmb.2019.03.009 .

Šošić-Jurjević, Branka , Lütjohann, Dieter, Renko, Kostja, Filipović, Branko, Radulović, Niko, Ajdžanović, Vladimir, Trifunović, Svetlana, Nestorović, Nataša, Živanović, Jasmina, Manojlović-Stojanoski, Milica, Kӧhrle, Josef, Milošević, Verica, "The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats." in The Journal of Steroid Biochemistry and Molecular Biology, 190 (2019):1-10,
https://doi.org/10.1016/j.jsbmb.2019.03.009 . .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Micić, M
AU  - Radulović, N
PY  - 1999
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1841
AB  - The effects of multiple treatment with estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) or a combination of the two on gonadotrophic cells in the pituitary pars distalis of middle-aged female rats were examined. The animals were treated daily for two weeks with EDP (0.625 mg i.p./kg body weight) or Ca (11.4 mg/kg body weight) or EDP+Ca. Luteinising (LH) and follicle stimulating hormone (FSH)-producing cells were examined by immunohistochemistry using antisera to the specific (beta) beta-subunits of LH and FSH and a peroxidase-anti-peroxidase immunohistochemical procedure. Plasma levels of FSH and LH were measured by radio-immune assay. A stereological method for determining morphometric parameters in immunopositive FSH and LH cells was used. The number of gonadotrophs per unit area (mm(2)), their cellular volume and relative volume densities, as well as plasma levels of FSH and LH, were decreased in all treated females in comparison with the controls. The most significant decrease of these parameters was observed in EDP-treated animals. Such changes were also expressed in Ca-treated animals, but the alterations were less distinct. These results demonstrate that multiple EDP or Ca application to middle-aged female rats is able to inhibit, directly or indirectly, the morphofunctional state of gonadotrophic cells in the pituitary pars distalis.
T2  - Histochemical Journal
T1  - Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats
IS  - 10
VL  - 31
EP  - 676
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1841
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Micić, M and Radulović, N",
year = "1999",
abstract = "The effects of multiple treatment with estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) or a combination of the two on gonadotrophic cells in the pituitary pars distalis of middle-aged female rats were examined. The animals were treated daily for two weeks with EDP (0.625 mg i.p./kg body weight) or Ca (11.4 mg/kg body weight) or EDP+Ca. Luteinising (LH) and follicle stimulating hormone (FSH)-producing cells were examined by immunohistochemistry using antisera to the specific (beta) beta-subunits of LH and FSH and a peroxidase-anti-peroxidase immunohistochemical procedure. Plasma levels of FSH and LH were measured by radio-immune assay. A stereological method for determining morphometric parameters in immunopositive FSH and LH cells was used. The number of gonadotrophs per unit area (mm(2)), their cellular volume and relative volume densities, as well as plasma levels of FSH and LH, were decreased in all treated females in comparison with the controls. The most significant decrease of these parameters was observed in EDP-treated animals. Such changes were also expressed in Ca-treated animals, but the alterations were less distinct. These results demonstrate that multiple EDP or Ca application to middle-aged female rats is able to inhibit, directly or indirectly, the morphofunctional state of gonadotrophic cells in the pituitary pars distalis.",
journal = "Histochemical Journal",
title = "Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats",
number = "10",
volume = "31",
pages = "676",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1841"
}

Lovren, M. B., Sekulić, M. I., Milosević, V., Micić, M.,& Radulović, N.. (1999). Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats. in Histochemical Journal, 31(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1841

Lovren MB, Sekulić MI, Milosević V, Micić M, Radulović N. Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats. in Histochemical Journal. 1999;31(10):null-676.
https://hdl.handle.net/21.15107/rcub_ibiss_1841 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Micić, M, Radulović, N, "Effects of estradiol and calcium on gonadotrophic cells in middle-aged female rats" in Histochemical Journal, 31, no. 10 (1999),
https://hdl.handle.net/21.15107/rcub_ibiss_1841 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Radulović, N
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1856
AB  - The effects of intracerebroventricular application of SRIF-14 and SRIF-28 on pituitary gonadotrophic cells (FSH and LH) were examined using immunocytochemical and morphometrical methods in adult female Wistar rats. FSH- and LH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were used to evaluate changes in the number, volume densities and relative volume densities of LH- and FSH-immunopositive cells. In females treated with SRIF-14 or SRIF-28, the gonadotrophs were smaller, often pycnotic and more intensely stained. The number of LH-positive cells per unit area (mm(2)) was significantly decreased in both somatostatin-treated groups, while the number of FSH-positive cells was similar to that in the controls. Volume densities of perykarya of LH- and FSH-positive cells were decreased in all treated females, but extremely different in LH-positive cells after SRIF-14 administration. The relative volume density of LH cells was significantly decreased in both somatostatin-treated groups, while immunopositive FSH cells were not significantly decreased compared with the controls. It can be concluded that centrally applied somatostatins lead to changes in the immunocytochemical and morphometric properties of LH cells, while there is no significant effect on FSH cells.
T2  - Acta Histochemica
T1  - Effects of somatostatins on gonadotrophic cells in female rats
IS  - 3
VL  - 100
EP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1856
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Radulović, N",
year = "1998",
abstract = "The effects of intracerebroventricular application of SRIF-14 and SRIF-28 on pituitary gonadotrophic cells (FSH and LH) were examined using immunocytochemical and morphometrical methods in adult female Wistar rats. FSH- and LH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were used to evaluate changes in the number, volume densities and relative volume densities of LH- and FSH-immunopositive cells. In females treated with SRIF-14 or SRIF-28, the gonadotrophs were smaller, often pycnotic and more intensely stained. The number of LH-positive cells per unit area (mm(2)) was significantly decreased in both somatostatin-treated groups, while the number of FSH-positive cells was similar to that in the controls. Volume densities of perykarya of LH- and FSH-positive cells were decreased in all treated females, but extremely different in LH-positive cells after SRIF-14 administration. The relative volume density of LH cells was significantly decreased in both somatostatin-treated groups, while immunopositive FSH cells were not significantly decreased compared with the controls. It can be concluded that centrally applied somatostatins lead to changes in the immunocytochemical and morphometric properties of LH cells, while there is no significant effect on FSH cells.",
journal = "Acta Histochemica",
title = "Effects of somatostatins on gonadotrophic cells in female rats",
number = "3",
volume = "100",
pages = "335",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1856"
}

Lovren, M. B., Sekulić, M. I., Milosević, V.,& Radulović, N.. (1998). Effects of somatostatins on gonadotrophic cells in female rats. in Acta Histochemica, 100(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1856

Lovren MB, Sekulić MI, Milosević V, Radulović N. Effects of somatostatins on gonadotrophic cells in female rats. in Acta Histochemica. 1998;100(3):null-335.
https://hdl.handle.net/21.15107/rcub_ibiss_1856 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Radulović, N, "Effects of somatostatins on gonadotrophic cells in female rats" in Acta Histochemica, 100, no. 3 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1856 .

TY  - JOUR
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Milosević, V
AU  - Micić, M
AU  - Radulović, N
PY  - 1997
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1877
AB  - Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment
IS  - 2
VL  - 16
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1877
ER  - 

@article{
author = "Lovren, Mirjana B. and Sekulić, Milka I. and Milosević, V and Micić, M and Radulović, N",
year = "1997",
abstract = "Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment",
number = "2",
volume = "16",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1877"
}

Lovren, M. B., Sekulić, M. I., Milosević, V., Micić, M.,& Radulović, N.. (1997). Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1877

Lovren MB, Sekulić MI, Milosević V, Micić M, Radulović N. Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 1997;16(2):null-87.
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .

Lovren, Mirjana B., Sekulić, Milka I., Milosević, V, Micić, M, Radulović, N, "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16, no. 2 (1997),
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .