TY  - JOUR
AU  - Nikolić, Sandra
AU  - Gazdić-Janković, Marina
AU  - Rosić, Gvozden
AU  - Miletić-Kovačević, Marina
AU  - Jovičić, Nemanja
AU  - Nestorović, Nataša
AU  - Stojković, Petra
AU  - Filipović, Nenad
AU  - Milošević-Đorđević, Olivera
AU  - Selaković, Dragica
AU  - Živanović, Marko
AU  - Seklić, Dragana
AU  - Milivojević, Nevena
AU  - Marković, Aleksandra
AU  - Seist, Richard
AU  - Vasilijić, Sasa
AU  - Stanković, Konstantina M.
AU  - Stojković, Miodrag
AU  - Ljujić, Biljana
PY  - 2022
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35405220
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4950
AB  - Commercially manufactured or generated through environmental degradation, microplastics (MPs) and nanoplastics (NPs) considerably contribute to environmental pollution. There is a knowledge gap in how exposure to MPs/NPs changes cellular function and affects animal and human health. Here, we demonstrate that after oral uptake, fluorescent polystyrene (PS) nanoparticles pass through the mouse digestive system, accumulate and aggregate in different organs, and induce functional changes in cells and organs. Using cochlear explant as a novel in vitro system, we confirmed the consequences of PS-MP/NP interaction with inner ear cells by detecting aggregates and hetero-aggregates of PS particles in hair cells. The testes of treated males accumulated MPs/NPs in the interstitial compartment surrounding the seminiferous tubules, which was associated with a statistically significant decrease in testosterone levels. Male mice showed increased secretion of interleukins (IL-12p35 and IL-23) by splenocytes while cyto- and genotoxicity tests indicated impaired cell viability and increased DNA damage in spleen tissue. Males also showed a broad range of anxiogenic responses to PS nanoparticles while hippocampal samples from treated females showed an increased expression of Bax and Nlrp3 genes, indicating a pro-apoptotic/proinflammatory effect of PS treatment. Taken together, induced PS effects are also gender-dependent, and therefore, strongly motivate future research to mitigate the deleterious effects of nanosized plastic particles.
PB  - Elsevier Ltd
T2  - Environmental Pollution
T1  - Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.
VL  - 305
DO  - 10.1016/j.envpol.2022.119206
SP  - 119206
ER  - 

@article{
author = "Nikolić, Sandra and Gazdić-Janković, Marina and Rosić, Gvozden and Miletić-Kovačević, Marina and Jovičić, Nemanja and Nestorović, Nataša and Stojković, Petra and Filipović, Nenad and Milošević-Đorđević, Olivera and Selaković, Dragica and Živanović, Marko and Seklić, Dragana and Milivojević, Nevena and Marković, Aleksandra and Seist, Richard and Vasilijić, Sasa and Stanković, Konstantina M. and Stojković, Miodrag and Ljujić, Biljana",
year = "2022",
abstract = "Commercially manufactured or generated through environmental degradation, microplastics (MPs) and nanoplastics (NPs) considerably contribute to environmental pollution. There is a knowledge gap in how exposure to MPs/NPs changes cellular function and affects animal and human health. Here, we demonstrate that after oral uptake, fluorescent polystyrene (PS) nanoparticles pass through the mouse digestive system, accumulate and aggregate in different organs, and induce functional changes in cells and organs. Using cochlear explant as a novel in vitro system, we confirmed the consequences of PS-MP/NP interaction with inner ear cells by detecting aggregates and hetero-aggregates of PS particles in hair cells. The testes of treated males accumulated MPs/NPs in the interstitial compartment surrounding the seminiferous tubules, which was associated with a statistically significant decrease in testosterone levels. Male mice showed increased secretion of interleukins (IL-12p35 and IL-23) by splenocytes while cyto- and genotoxicity tests indicated impaired cell viability and increased DNA damage in spleen tissue. Males also showed a broad range of anxiogenic responses to PS nanoparticles while hippocampal samples from treated females showed an increased expression of Bax and Nlrp3 genes, indicating a pro-apoptotic/proinflammatory effect of PS treatment. Taken together, induced PS effects are also gender-dependent, and therefore, strongly motivate future research to mitigate the deleterious effects of nanosized plastic particles.",
publisher = "Elsevier Ltd",
journal = "Environmental Pollution",
title = "Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.",
volume = "305",
doi = "10.1016/j.envpol.2022.119206",
pages = "119206"
}

Nikolić, S., Gazdić-Janković, M., Rosić, G., Miletić-Kovačević, M., Jovičić, N., Nestorović, N., Stojković, P., Filipović, N., Milošević-Đorđević, O., Selaković, D., Živanović, M., Seklić, D., Milivojević, N., Marković, A., Seist, R., Vasilijić, S., Stanković, K. M., Stojković, M.,& Ljujić, B.. (2022). Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.. in Environmental Pollution
Elsevier Ltd., 305, 119206.
https://doi.org/10.1016/j.envpol.2022.119206

Nikolić S, Gazdić-Janković M, Rosić G, Miletić-Kovačević M, Jovičić N, Nestorović N, Stojković P, Filipović N, Milošević-Đorđević O, Selaković D, Živanović M, Seklić D, Milivojević N, Marković A, Seist R, Vasilijić S, Stanković KM, Stojković M, Ljujić B. Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.. in Environmental Pollution. 2022;305:119206.
doi:10.1016/j.envpol.2022.119206 .

Nikolić, Sandra, Gazdić-Janković, Marina, Rosić, Gvozden, Miletić-Kovačević, Marina, Jovičić, Nemanja, Nestorović, Nataša, Stojković, Petra, Filipović, Nenad, Milošević-Đorđević, Olivera, Selaković, Dragica, Živanović, Marko, Seklić, Dragana, Milivojević, Nevena, Marković, Aleksandra, Seist, Richard, Vasilijić, Sasa, Stanković, Konstantina M., Stojković, Miodrag, Ljujić, Biljana, "Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice." in Environmental Pollution, 305 (2022):119206,
https://doi.org/10.1016/j.envpol.2022.119206 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Vasilijić, Sasa R
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/961
AB  - Allergic contact dermatitis (ACD) is a common skin inflammatory disease that develops in hosts sensitized with contact allergens. Elucidation of dose-response relationships represents one of the approaches in studying the type of ACD in humans/animal models, termed as contact hyper-sensitivity reaction (CHS). Such studies have demonstrated that the intensity of sensitization determines the response to elicitation with a contact allergen, but underlying mechanisms are unclear. The aim of this study is to explore the impact of the sensitization on contact hypersensitivity to dinitrochlorobenzene (DNCB) in rats by measuring the incidence and intensity of a challenge response in hosts sensitized with two different doses (i.e. low and high) of this hapten. Assumptions concerning the contribution from the magnitude of sensitization doses were drawn on the basis of effects from the two doses on the measured reaction parameters. Ear swelling and activity of lymph nodes that drain challenged skin (cdLN), including cellularity, proliferation, and effector cytokine IFN gamma and IL-17 production was measured in rats sensitized with 0.4% or 4% DNCB and challenged with a non-irritant (0.13%) dose. Sensitization with 4% DNCB resulted in a greater proportion of rats who responded more intensely (than unsensitized challenged rats) to challenge in terms of ear swelling and increases in cdLN activity (except for IFN gamma). The intensity of cdLN responses was higher in these hosts as well. Among the high-dose-sensitized rats, greater cellularity/proliferation of cells from lymph nodes (sdLN) that drain the high-dose-sensitized skin, as well as higher IL-17 production, was noted compared to what was seen in rats that received low-dose sensitization. In contrast, unchanged spontaneous and even decreased hapten-stimulated IFN gamma production after the high DNCB dose was observed. Based on the data, it seems the impact of magnitude of sensitization dose on CHS might be related to the rise in the proportion of rats that responded to challenge with an increase of dLN activity. Coincidental higher production of IL-17 by dLN cells from the high-dose-sensitized rats and following challenge of these hosts underscored the significance of IL-17 for a CHS to DNCB.
T2  - Journal of Immunotoxicology
T1  - Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats
IS  - 4
VL  - 10
SP  - 165
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_961
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Vasilijić, Sasa R and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Allergic contact dermatitis (ACD) is a common skin inflammatory disease that develops in hosts sensitized with contact allergens. Elucidation of dose-response relationships represents one of the approaches in studying the type of ACD in humans/animal models, termed as contact hyper-sensitivity reaction (CHS). Such studies have demonstrated that the intensity of sensitization determines the response to elicitation with a contact allergen, but underlying mechanisms are unclear. The aim of this study is to explore the impact of the sensitization on contact hypersensitivity to dinitrochlorobenzene (DNCB) in rats by measuring the incidence and intensity of a challenge response in hosts sensitized with two different doses (i.e. low and high) of this hapten. Assumptions concerning the contribution from the magnitude of sensitization doses were drawn on the basis of effects from the two doses on the measured reaction parameters. Ear swelling and activity of lymph nodes that drain challenged skin (cdLN), including cellularity, proliferation, and effector cytokine IFN gamma and IL-17 production was measured in rats sensitized with 0.4% or 4% DNCB and challenged with a non-irritant (0.13%) dose. Sensitization with 4% DNCB resulted in a greater proportion of rats who responded more intensely (than unsensitized challenged rats) to challenge in terms of ear swelling and increases in cdLN activity (except for IFN gamma). The intensity of cdLN responses was higher in these hosts as well. Among the high-dose-sensitized rats, greater cellularity/proliferation of cells from lymph nodes (sdLN) that drain the high-dose-sensitized skin, as well as higher IL-17 production, was noted compared to what was seen in rats that received low-dose sensitization. In contrast, unchanged spontaneous and even decreased hapten-stimulated IFN gamma production after the high DNCB dose was observed. Based on the data, it seems the impact of magnitude of sensitization dose on CHS might be related to the rise in the proportion of rats that responded to challenge with an increase of dLN activity. Coincidental higher production of IL-17 by dLN cells from the high-dose-sensitized rats and following challenge of these hosts underscored the significance of IL-17 for a CHS to DNCB.",
journal = "Journal of Immunotoxicology",
title = "Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats",
number = "4",
volume = "10",
pages = "165-360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_961"
}

Popov Aleksandrov, A., Mirkov, I., Vasilijić, S. R., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2013). Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats. in Journal of Immunotoxicology, 10(4), 165-360.
https://hdl.handle.net/21.15107/rcub_ibiss_961

Popov Aleksandrov A, Mirkov I, Vasilijić SR, Zolotarevski LD, Kataranovski DS, Kataranovski M. Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats. in Journal of Immunotoxicology. 2013;10(4):165-360.
https://hdl.handle.net/21.15107/rcub_ibiss_961 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Vasilijić, Sasa R, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats" in Journal of Immunotoxicology, 10, no. 4 (2013):165-360,
https://hdl.handle.net/21.15107/rcub_ibiss_961 .