TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad
AU  - Gržetić, Ivan
AU  - Blagojević, Duško
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32777467
UR  - https://radar.ibiss.bg.ac.rs/123456789/3861
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.
VL  - 239
DO  - 10.1016/j.cbpc.2020.108852
SP  - 108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana and Purać, Jelena and Kojić, Danijela and Zarić, Nenad and Gržetić, Ivan and Blagojević, Duško and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.",
volume = "239",
doi = "10.1016/j.cbpc.2020.108852",
pages = "108852"
}

Ilijević, K., Vujanović, D., Orčić, S., Purać, J., Kojić, D., Zarić, N., Gržetić, I., Blagojević, D.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239, 108852.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Gržetić I, Blagojević D, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239:108852.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana, Purać, Jelena, Kojić, Danijela, Zarić, Nenad, Gržetić, Ivan, Blagojević, Duško, Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph." in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021):108852,
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - JOUR
AU  - Kojić, Danijela K.
AU  - Purać, Jelena S.
AU  - Nikolić, Tatjana V.
AU  - Orčić, Snežana M.
AU  - Vujanović, Dragana
AU  - Ilijević, Konstantin
AU  - Vukašinović, Elvira L.
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.schweizerbart.de/papers/entomologia/detail/39/91600/Oxidative_stress_and_the_activity_of_antioxidative?af=crossref
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3453
AB  - Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.
T2  - Entomologia Generalis
T1  - Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees
IS  - 1
VL  - 39
DO  - 10.1127/entomologia/2019/0743
SP  - 33
EP  - 44
ER  - 

@article{
author = "Kojić, Danijela K. and Purać, Jelena S. and Nikolić, Tatjana V. and Orčić, Snežana M. and Vujanović, Dragana and Ilijević, Konstantin and Vukašinović, Elvira L. and Blagojević, Duško",
year = "2019",
abstract = "Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.",
journal = "Entomologia Generalis",
title = "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees",
number = "1",
volume = "39",
doi = "10.1127/entomologia/2019/0743",
pages = "33-44"
}

Kojić, D. K., Purać, J. S., Nikolić, T. V., Orčić, S. M., Vujanović, D., Ilijević, K., Vukašinović, E. L.,& Blagojević, D.. (2019). Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis, 39(1), 33-44.
https://doi.org/10.1127/entomologia/2019/0743

Kojić DK, Purać JS, Nikolić TV, Orčić SM, Vujanović D, Ilijević K, Vukašinović EL, Blagojević D. Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis. 2019;39(1):33-44.
doi:10.1127/entomologia/2019/0743 .

Kojić, Danijela K., Purać, Jelena S., Nikolić, Tatjana V., Orčić, Snežana M., Vujanović, Dragana, Ilijević, Konstantin, Vukašinović, Elvira L., Blagojević, Duško, "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees" in Entomologia Generalis, 39, no. 1 (2019):33-44,
https://doi.org/10.1127/entomologia/2019/0743 . .