TY  - JOUR
AU  - Fernandes, Angela
AU  - Polyzos, Nikolaos
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6399
AB  - The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
IS  - 7
VL  - 9
DO  - 10.3390/horticulturae9070838
SP  - 838
ER  - 

@article{
author = "Fernandes, Angela and Polyzos, Nikolaos and Mandim, Filipa and Pereira, Carla and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon",
year = "2023",
abstract = "The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants",
number = "7",
volume = "9",
doi = "10.3390/horticulturae9070838",
pages = "838"
}

Fernandes, A., Polyzos, N., Mandim, F., Pereira, C., Petrović, J., Soković, M.,& Petropoulos, S.. (2023). Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae
Basel: MDPI., 9(7), 838.
https://doi.org/10.3390/horticulturae9070838

Fernandes A, Polyzos N, Mandim F, Pereira C, Petrović J, Soković M, Petropoulos S. Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae. 2023;9(7):838.
doi:10.3390/horticulturae9070838 .

Fernandes, Angela, Polyzos, Nikolaos, Mandim, Filipa, Pereira, Carla, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants" in Horticulturae, 9, no. 7 (2023):838,
https://doi.org/10.3390/horticulturae9070838 . .

TY  - CONF
AU  - Stojković, Dejan
AU  - Kostić, Marina
AU  - Fernandes, Angela
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5157
AB  - Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5157
ER  - 

@conference{
author = "Stojković, Dejan and Kostić, Marina and Fernandes, Angela and Barros, Lillian and Glamočlija, Jasmina",
year = "2022",
abstract = "Craterellus cornucopioides (L.) Pers. (Cantharellaceae), or horn of plenty, is an edible fungi. It is also known as the black chanterelle, black trumpet, crna truba (Serbian), trompette de la mort (French), trombetta dei morti (Italian) or trumpet of the dead, djondjon (Haitian). C. cornucopioides growing wild in Serbia was chemically characterized in nutritional value and individual compounds (sugars, fatty acids, tocopherols, organic acids and phenolic compounds). Antioxidant (scavenging activity, reducing power and inhibition of lipid peroxidation), antibacterial and antifungal activities of its methanolic extract were evaluated under in vitro conditions. C. cornucopioides was showed to be rich in carbohydrates (79.32 g/100g dw), followed by proteins (8.36 g/100g dw), ash (9.43 g/100g dw) and fat (2.90 g/100g dw). The most abundant sugar compound was identified as mannitol (14.16 g/100g dw); four tocopherol isoforms (α-, β-,γ- and δ-tocopherols) were detected. Unsaturated fatty acids predominated over saturated fatty acids, with oleic and linoleic acids as the most dominant ones. Five organic acids were quantified: oxalic, citric, quinic, and fumaric and malic acids. The methanolic extract of the mushroom showed in vitro antioxidant and antimicrobial activities. Methanolic extract successively inhibited growth of pathogenic bacteria.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5157"
}

Stojković, D., Kostić, M., Fernandes, A., Barros, L.,& Glamočlija, J.. (2022). Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157

Stojković D, Kostić M, Fernandes A, Barros L, Glamočlija J. Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

Stojković, Dejan, Kostić, Marina, Fernandes, Angela, Barros, Lillian, Glamočlija, Jasmina, "Nutritional properties, chemical composition and biological properties of Craterellus cornucopioides" in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):168,
https://hdl.handle.net/21.15107/rcub_ibiss_5157 .

TY  - CONF
AU  - Petrović, Jovana
AU  - Fernandes, Ângela
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
AU  - Shekhar, Aditya
AU  - Glamočlija, Jasmina
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4921
AB  - Edible mushrooms have been appreciated globally for their organoleptic, nutritional and chemical
properties. In the present study, fruiting body of Calocybe gambosa , wild growing in Serbia has been
chemically characterized (content of macronutrients, soluble sugars, tocopherols, fatty and organic acids)
and its bioactive properties (antimicrobial and antioxidant) evaluated. Obtained results suggest that this
mushroom is a source of carbohydrates and proteins, with low fat content. Sugar analysis revealed
presence of trehalose and mannitol. Tocopherol composition revealed presence of α-tocopherol, while
fatty acid analysis revealed presence of 24 fatty acids with prevalence of polyunsaturated fatty acids.
Amongst organic acids, oxalic, quinic, malic, citric and fumaric acid were detected. Comprehensive
antioxidant analysis (reducing power, DPPH scavenging activity, β -caroten/linoleic acid and TBARS
assay) indicate that mushroom is a perspective antioxidant, whereas its antimicrobial potential turned out
to be moderate. Nevertheless, at sub-inhibitory level methanolic extract disrupted cell-to-cell
communication using Pseudomonas aeruginosa PAO1 as a model system.
Finally, enrichment of oatmeal cookies with C. gambosa flakes not only improved nutritional value of
cookies, but was praised among participants in sensory evaluation test ( Table 1 ), indicating that along
with results of chemical composition and biological activity, this mushroom has potential to be regarded
as functional food.
PB  - Bragança: Instituto Politécnico de Bragança
C3  - 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
T1  - Calocybe gambosa (Fr.) Donk wild growing in Serbia as functionl ingredient in oatmeal
SP  - 206
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4921
ER  - 

@conference{
author = "Petrović, Jovana and Fernandes, Ângela and Stojković, Dejan and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R. and Shekhar, Aditya and Glamočlija, Jasmina",
year = "2021",
abstract = "Edible mushrooms have been appreciated globally for their organoleptic, nutritional and chemical
properties. In the present study, fruiting body of Calocybe gambosa , wild growing in Serbia has been
chemically characterized (content of macronutrients, soluble sugars, tocopherols, fatty and organic acids)
and its bioactive properties (antimicrobial and antioxidant) evaluated. Obtained results suggest that this
mushroom is a source of carbohydrates and proteins, with low fat content. Sugar analysis revealed
presence of trehalose and mannitol. Tocopherol composition revealed presence of α-tocopherol, while
fatty acid analysis revealed presence of 24 fatty acids with prevalence of polyunsaturated fatty acids.
Amongst organic acids, oxalic, quinic, malic, citric and fumaric acid were detected. Comprehensive
antioxidant analysis (reducing power, DPPH scavenging activity, β -caroten/linoleic acid and TBARS
assay) indicate that mushroom is a perspective antioxidant, whereas its antimicrobial potential turned out
to be moderate. Nevertheless, at sub-inhibitory level methanolic extract disrupted cell-to-cell
communication using Pseudomonas aeruginosa PAO1 as a model system.
Finally, enrichment of oatmeal cookies with C. gambosa flakes not only improved nutritional value of
cookies, but was praised among participants in sensory evaluation test ( Table 1 ), indicating that along
with results of chemical composition and biological activity, this mushroom has potential to be regarded
as functional food.",
publisher = "Bragança: Instituto Politécnico de Bragança",
journal = "1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online",
title = "Calocybe gambosa (Fr.) Donk wild growing in Serbia as functionl ingredient in oatmeal",
pages = "206",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4921"
}

Petrović, J., Fernandes, Â., Stojković, D., Soković, M., Barros, L., Ferreira, I. C.F.R., Shekhar, A.,& Glamočlija, J.. (2021). Calocybe gambosa (Fr.) Donk wild growing in Serbia as functionl ingredient in oatmeal. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
Bragança: Instituto Politécnico de Bragança., 206.
https://hdl.handle.net/21.15107/rcub_ibiss_4921

Petrović J, Fernandes Â, Stojković D, Soković M, Barros L, Ferreira IC, Shekhar A, Glamočlija J. Calocybe gambosa (Fr.) Donk wild growing in Serbia as functionl ingredient in oatmeal. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online. 2021;:206.
https://hdl.handle.net/21.15107/rcub_ibiss_4921 .

Petrović, Jovana, Fernandes, Ângela, Stojković, Dejan, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., Shekhar, Aditya, Glamočlija, Jasmina, "Calocybe gambosa (Fr.) Donk wild growing in Serbia as functionl ingredient in oatmeal" in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online (2021):206,
https://hdl.handle.net/21.15107/rcub_ibiss_4921 .

TY  - JOUR
AU  - Fernandes, Ângela
AU  - Petrović, Jovana
AU  - Stojković, Dejan
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Martins, Anabela
AU  - Ferreira, Isabel
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6414
AB  - Wild mushrooms are mainly collected during the rainy season and valued as a nutritious food and
sources of natural medicines and nutraceuticals. The aim of this study was to determine the chemical
composition and bioactive properties (antioxidant, antimicrobial and cytotoxicity) of Polyporus squa mosus from two different origins, Portugal and Serbia. The sample from Portugal showed higher contents
of as protein (17.14 g/100 g), fat (2.69 g/100 g), ash (3.15 g/100 g) and carbohydrates (77.02 g/100 g); the
same sample gave the highest antioxidant activity: highest reducing power, DPPH radical scavenging
activity, and lipid peroxidation inhibition in both b-carotene/linoleate and TBARS assay. These results
could be related to its higher content in total tocopherols (1968.65 mg/100 g) and phenolic compounds
(1.29 mg/100 g). Both extracts exhibited antibacterial activity against all the tested organisms. The
samples from Serbia gave higher overall antibacterial activity and showed excellent antibiofilm activity
(88.30%). Overall, P. squamosus methanolic extracts possessed antioxidant, antimicrobial, antibiofilm and
anti-quorum sensing activity, and without toxicity for liver cells. This investigation highlights alterna tives to be explored for the treatment of bacterial infections, in particular against Pseudomonas aerugi nosa. This study provides important results for the chemical and bioactive properties, especially
antimicrobial activity of the mushroom P. squamosus. Moreover, to the authors’ knowledge this is the first
report on sugars, organic acids, and individual phenolic compounds in P. squamosus.
PB  - Elsevier
T2  - LWT - Food Science and Technology
T1  - Polyporus squamosus (Huds.) Fr from different origins: Chemical characterization, screening of the bioactive properties and specific antimicrobial effects against Pseudomonas aeruginosa.
VL  - 69
DO  - 10.1016/j.lwt.2016.01.037
SP  - 91
EP  - 97
ER  - 

@article{
author = "Fernandes, Ângela and Petrović, Jovana and Stojković, Dejan and Barros, Lillian and Glamočlija, Jasmina and Soković, Marina and Martins, Anabela and Ferreira, Isabel",
year = "2016",
abstract = "Wild mushrooms are mainly collected during the rainy season and valued as a nutritious food and
sources of natural medicines and nutraceuticals. The aim of this study was to determine the chemical
composition and bioactive properties (antioxidant, antimicrobial and cytotoxicity) of Polyporus squa mosus from two different origins, Portugal and Serbia. The sample from Portugal showed higher contents
of as protein (17.14 g/100 g), fat (2.69 g/100 g), ash (3.15 g/100 g) and carbohydrates (77.02 g/100 g); the
same sample gave the highest antioxidant activity: highest reducing power, DPPH radical scavenging
activity, and lipid peroxidation inhibition in both b-carotene/linoleate and TBARS assay. These results
could be related to its higher content in total tocopherols (1968.65 mg/100 g) and phenolic compounds
(1.29 mg/100 g). Both extracts exhibited antibacterial activity against all the tested organisms. The
samples from Serbia gave higher overall antibacterial activity and showed excellent antibiofilm activity
(88.30%). Overall, P. squamosus methanolic extracts possessed antioxidant, antimicrobial, antibiofilm and
anti-quorum sensing activity, and without toxicity for liver cells. This investigation highlights alterna tives to be explored for the treatment of bacterial infections, in particular against Pseudomonas aerugi nosa. This study provides important results for the chemical and bioactive properties, especially
antimicrobial activity of the mushroom P. squamosus. Moreover, to the authors’ knowledge this is the first
report on sugars, organic acids, and individual phenolic compounds in P. squamosus.",
publisher = "Elsevier",
journal = "LWT - Food Science and Technology",
title = "Polyporus squamosus (Huds.) Fr from different origins: Chemical characterization, screening of the bioactive properties and specific antimicrobial effects against Pseudomonas aeruginosa.",
volume = "69",
doi = "10.1016/j.lwt.2016.01.037",
pages = "91-97"
}

Fernandes, Â., Petrović, J., Stojković, D., Barros, L., Glamočlija, J., Soković, M., Martins, A.,& Ferreira, I.. (2016). Polyporus squamosus (Huds.) Fr from different origins: Chemical characterization, screening of the bioactive properties and specific antimicrobial effects against Pseudomonas aeruginosa.. in LWT - Food Science and Technology
Elsevier., 69, 91-97.
https://doi.org/10.1016/j.lwt.2016.01.037

Fernandes Â, Petrović J, Stojković D, Barros L, Glamočlija J, Soković M, Martins A, Ferreira I. Polyporus squamosus (Huds.) Fr from different origins: Chemical characterization, screening of the bioactive properties and specific antimicrobial effects against Pseudomonas aeruginosa.. in LWT - Food Science and Technology. 2016;69:91-97.
doi:10.1016/j.lwt.2016.01.037 .

Fernandes, Ângela, Petrović, Jovana, Stojković, Dejan, Barros, Lillian, Glamočlija, Jasmina, Soković, Marina, Martins, Anabela, Ferreira, Isabel, "Polyporus squamosus (Huds.) Fr from different origins: Chemical characterization, screening of the bioactive properties and specific antimicrobial effects against Pseudomonas aeruginosa." in LWT - Food Science and Technology, 69 (2016):91-97,
https://doi.org/10.1016/j.lwt.2016.01.037 . .

TY  - JOUR
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Nikolić, Miloš
AU  - Fernandes, Angela
AU  - Barros, Lillian
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
AU  - van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1989
AB  - Ethnopharmacological relevance: In Russian traditional medicine, an
   extract from the mushroom Inonotus obliquus (Fr.) Pil at is used as an
   anti-tumor medicine and diuretic. It has been reported that Inonotus
   obliquus has therapeutic effects, such as anti-inflammatory,
   immuno-modulatory and hepatoprotective effects. This study was designed
   to investigate the chemical composition and biological properties of
   aqueous and ethanolic extracts of Inonotus obliquus from Finland,
   Russia, and Thailand. Their antioxidative, antimicrobial, and antiquorum
   properties were tested as well as the cytotoxicity on various tumor cell
   lines.
   Materials and methods: The tested extract was subjected to conventional
   chemical study to identified organic acids and phenolic compounds.
   Antioxidative activity was measured by several different assays.
   Antimicrobial potential of extracts was tested by microdilution method,
   and antiquorum sensing activity and antibiofilm formation of Inonotus
   obliquus extracts was tested on Pseudomonas aeruginosa. Cytotoxicity of
   the extracts was tested on tumor cells (MCF-7, NCI-H460, HeLa and
   HepG(2)) and non-tumor liver cells primary cultures.
   Results: Oxalic acid was found as the main organic acid, with the
   highest amount in the aqueous extract from Russia. Gallic,
   protocatechuic and p-hydroxybenzoic acids were detected in all samples.
   Inonotus obliquus extracts showed high antioxidant and antimicrobial
   activity. Extracts were tested at subMIC for anti-quorum sensing (AQS)
   activity in Pseudomonas aeruginosa and all extracts showed definite AQS
   activity. The assays were done using twitching and swarming of bacterial
   cultures, and the amount of produced pyocyanin as QS parameters. All the
   extracts demonstrated cytotoxic effect on four tumor cell lines and not
   on primary porcine liver cells PLP2.
   Conclusions: As the Inonotus obliquus presence in Chaga conks is
   limited, further purification is necessary to draw quantitative
   conclusions. The presence of AQS activity in medicinal mushrooms
   suggests a broader anti-infectious disease protection than only
   immunomodulatory effects. (C) 2015 Elsevier Ireland Ltd. All rights
   reserved.
T2  - Journal of Ethnopharmacology
T1  - Chemical characterization and biological activity of Chaga (Inonotus
 obliquus), a medicinal "mushroom"
VL  - 162
DO  - 10.1016/j.jep.2014.12.069
SP  - 323
EP  - 332
ER  - 

@article{
author = "Glamočlija, Jasmina and Ćirić, Ana and Nikolić, Miloš and Fernandes, Angela and Barros, Lillian and Calhelha, Ricardo C. and Ferreira, Isabel C. F. R. and Soković, Marina and van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Ethnopharmacological relevance: In Russian traditional medicine, an
   extract from the mushroom Inonotus obliquus (Fr.) Pil at is used as an
   anti-tumor medicine and diuretic. It has been reported that Inonotus
   obliquus has therapeutic effects, such as anti-inflammatory,
   immuno-modulatory and hepatoprotective effects. This study was designed
   to investigate the chemical composition and biological properties of
   aqueous and ethanolic extracts of Inonotus obliquus from Finland,
   Russia, and Thailand. Their antioxidative, antimicrobial, and antiquorum
   properties were tested as well as the cytotoxicity on various tumor cell
   lines.
   Materials and methods: The tested extract was subjected to conventional
   chemical study to identified organic acids and phenolic compounds.
   Antioxidative activity was measured by several different assays.
   Antimicrobial potential of extracts was tested by microdilution method,
   and antiquorum sensing activity and antibiofilm formation of Inonotus
   obliquus extracts was tested on Pseudomonas aeruginosa. Cytotoxicity of
   the extracts was tested on tumor cells (MCF-7, NCI-H460, HeLa and
   HepG(2)) and non-tumor liver cells primary cultures.
   Results: Oxalic acid was found as the main organic acid, with the
   highest amount in the aqueous extract from Russia. Gallic,
   protocatechuic and p-hydroxybenzoic acids were detected in all samples.
   Inonotus obliquus extracts showed high antioxidant and antimicrobial
   activity. Extracts were tested at subMIC for anti-quorum sensing (AQS)
   activity in Pseudomonas aeruginosa and all extracts showed definite AQS
   activity. The assays were done using twitching and swarming of bacterial
   cultures, and the amount of produced pyocyanin as QS parameters. All the
   extracts demonstrated cytotoxic effect on four tumor cell lines and not
   on primary porcine liver cells PLP2.
   Conclusions: As the Inonotus obliquus presence in Chaga conks is
   limited, further purification is necessary to draw quantitative
   conclusions. The presence of AQS activity in medicinal mushrooms
   suggests a broader anti-infectious disease protection than only
   immunomodulatory effects. (C) 2015 Elsevier Ireland Ltd. All rights
   reserved.",
journal = "Journal of Ethnopharmacology",
title = "Chemical characterization and biological activity of Chaga (Inonotus
 obliquus), a medicinal "mushroom"",
volume = "162",
doi = "10.1016/j.jep.2014.12.069",
pages = "323-332"
}

Glamočlija, J., Ćirić, A., Nikolić, M., Fernandes, A., Barros, L., Calhelha, R. C., Ferreira, I. C. F. R., Soković, M.,& van Griensven, L. J. L. D.. (2015). Chemical characterization and biological activity of Chaga (Inonotus
 obliquus), a medicinal "mushroom". in Journal of Ethnopharmacology, 162, 323-332.
https://doi.org/10.1016/j.jep.2014.12.069

Glamočlija J, Ćirić A, Nikolić M, Fernandes A, Barros L, Calhelha RC, Ferreira ICFR, Soković M, van Griensven LJLD. Chemical characterization and biological activity of Chaga (Inonotus
 obliquus), a medicinal "mushroom". in Journal of Ethnopharmacology. 2015;162:323-332.
doi:10.1016/j.jep.2014.12.069 .

Glamočlija, Jasmina, Ćirić, Ana, Nikolić, Miloš, Fernandes, Angela, Barros, Lillian, Calhelha, Ricardo C., Ferreira, Isabel C. F. R., Soković, Marina, van Griensven, Leo J. L. D., "Chemical characterization and biological activity of Chaga (Inonotus
 obliquus), a medicinal "mushroom"" in Journal of Ethnopharmacology, 162 (2015):323-332,
https://doi.org/10.1016/j.jep.2014.12.069 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Nikolić, Miloš
AU  - Ćirić, Ana
AU  - Fernandes, Angela
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2267
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3638
AB  - Agrocybe aegerita (Brig.) Sing is a basidiomycete, white rot fungus.   Antimicrobial activities and the antiqourum effect on Pseudomonas   aeruginosa of an A. aegerita methanolic extract were investigated. The   extract showed very good antimicrobial activity against all the tested   microorganisms in a dose dependent manner. Effects of the Sub-MIC, MIC   and 2MIC of the A. aegerita methanolic extract regulated the virulence   factors in the quorum sensing (QS) test, as well as biofilm formation on   P. aeruginosa. Sub-inhibitory and inhibitory concentrations of the   extract demonstrated the reduction of virulence factors such as   pyocyanin production, twitching and swimming motility. The biofilm   forming capability of P. aeruginosa PAO1 was also reduced in a   concentration-dependent manner. Furthermore, the chemical composition of   the methanolic extract was determined considering its phenolic   composition. The methanolic extract of A. aegerita can be a very good   source of bioactive substances. This research is of great importance due   to the prevalence of drug-resistant microorganisms.
T2  - Food & Function
T1  - Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa
IS  - 12
VL  - 5
DO  - 10.1039/c4fo00819g
SP  - 3296
EP  - 3303
ER  - 

@article{
author = "Petrović, Jovana and Glamočlija, Jasmina and Stojković, Dejan and Nikolić, Miloš and Ćirić, Ana and Fernandes, Angela and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2014",
abstract = "Agrocybe aegerita (Brig.) Sing is a basidiomycete, white rot fungus.   Antimicrobial activities and the antiqourum effect on Pseudomonas   aeruginosa of an A. aegerita methanolic extract were investigated. The   extract showed very good antimicrobial activity against all the tested   microorganisms in a dose dependent manner. Effects of the Sub-MIC, MIC   and 2MIC of the A. aegerita methanolic extract regulated the virulence   factors in the quorum sensing (QS) test, as well as biofilm formation on   P. aeruginosa. Sub-inhibitory and inhibitory concentrations of the   extract demonstrated the reduction of virulence factors such as   pyocyanin production, twitching and swimming motility. The biofilm   forming capability of P. aeruginosa PAO1 was also reduced in a   concentration-dependent manner. Furthermore, the chemical composition of   the methanolic extract was determined considering its phenolic   composition. The methanolic extract of A. aegerita can be a very good   source of bioactive substances. This research is of great importance due   to the prevalence of drug-resistant microorganisms.",
journal = "Food & Function",
title = "Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa",
number = "12",
volume = "5",
doi = "10.1039/c4fo00819g",
pages = "3296-3303"
}

Petrović, J., Glamočlija, J., Stojković, D., Nikolić, M., Ćirić, A., Fernandes, A., Ferreira, I. C. F. R.,& Soković, M.. (2014). Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa. in Food & Function, 5(12), 3296-3303.
https://doi.org/10.1039/c4fo00819g

Petrović J, Glamočlija J, Stojković D, Nikolić M, Ćirić A, Fernandes A, Ferreira ICFR, Soković M. Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa. in Food & Function. 2014;5(12):3296-3303.
doi:10.1039/c4fo00819g .

Petrović, Jovana, Glamočlija, Jasmina, Stojković, Dejan, Nikolić, Miloš, Ćirić, Ana, Fernandes, Angela, Ferreira, Isabel C. F. R., Soković, Marina, "Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa" in Food & Function, 5, no. 12 (2014):3296-3303,
https://doi.org/10.1039/c4fo00819g . .

TY  - JOUR
AU  - Nikolić, Miloš
AU  - Glamočlija, Jasmina
AU  - Ferreira, Isabel C. F. R.
AU  - Calhelha, Ricardo C.
AU  - Fernandes, Angela
AU  - Markovic, Tatjana
AU  - Markovic, Dejan
AU  - Giweli, Abdulhamed
AU  - Soković, Marina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2300
AB  - Aromatic plant species of genus Thymus are important medicinal plants,
   highly recommended due to a range of therapeutic properties of their
   essential oils, commonly known as thyme oil: antirheumatic, antiseptic,
   antispasmodic, antimicrobial, cardiac, carminative, diuretic and
   expectorant. The oil is also beneficial in boosting the immune system
   and helps to fight colds, flu, infectious diseases and chills. It is
   proved to be a urinary antiseptic, being very helpful for cystitis and
   urethritis. Scientific validation of traditional uses, and phytochemical
   and bioactivity evaluation of essential oils from Thymus serpyllum,
   Thymus algeriensis and Thymus vulgaris were performed.
   GC/MS analysis revealed thymol as a major component of T. algeriensis,
   T. vulgaris and T. serpyllum, with its contribution to the oil 56.0\%,
   48.9\% and 38.5\%, respectively. All three essential oils (EOs)
   exhibited a significant antimicrobial activity against all tested
   strains, the T. serpyllum oil being the most potent one (MIC 2.5-5 mu
   g/mL, MBC 5-10 mu g/mL; MIC 1-2 mu g/mL, MFC 2-4 mu g/mL). In addition,
   the T. serpyllum oil revealed the highest antioxidant activity in all
   conducted assays and it was the most effective one against all tested
   cell lines, presenting GI(50) values of 7.02-52.69 mu g/mL. Moreover,
   none of the EOs showed toxicity at tested concentrations (>400 mu g/mL)
   for porcine liver primary cell culture. In addition to their well-known
   traditional use in food and cosmetics, the great potential of tested
   Thymus essential oils for application in oral diseases and anticancer
   treatments, encourage further investigation. (C) 2013 Elsevier B.V. All
   rights reserved.
T2  - Industrial Crops and Products
T1  - Chemical composition, antimicrobial, antioxidant and antitumor activity
 of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus
 vulgaris L. essential oils
VL  - 52
DO  - 10.1016/j.indcrop.2013.10.006
SP  - 183
EP  - 190
ER  - 

@article{
author = "Nikolić, Miloš and Glamočlija, Jasmina and Ferreira, Isabel C. F. R. and Calhelha, Ricardo C. and Fernandes, Angela and Markovic, Tatjana and Markovic, Dejan and Giweli, Abdulhamed and Soković, Marina",
year = "2014",
abstract = "Aromatic plant species of genus Thymus are important medicinal plants,
   highly recommended due to a range of therapeutic properties of their
   essential oils, commonly known as thyme oil: antirheumatic, antiseptic,
   antispasmodic, antimicrobial, cardiac, carminative, diuretic and
   expectorant. The oil is also beneficial in boosting the immune system
   and helps to fight colds, flu, infectious diseases and chills. It is
   proved to be a urinary antiseptic, being very helpful for cystitis and
   urethritis. Scientific validation of traditional uses, and phytochemical
   and bioactivity evaluation of essential oils from Thymus serpyllum,
   Thymus algeriensis and Thymus vulgaris were performed.
   GC/MS analysis revealed thymol as a major component of T. algeriensis,
   T. vulgaris and T. serpyllum, with its contribution to the oil 56.0\%,
   48.9\% and 38.5\%, respectively. All three essential oils (EOs)
   exhibited a significant antimicrobial activity against all tested
   strains, the T. serpyllum oil being the most potent one (MIC 2.5-5 mu
   g/mL, MBC 5-10 mu g/mL; MIC 1-2 mu g/mL, MFC 2-4 mu g/mL). In addition,
   the T. serpyllum oil revealed the highest antioxidant activity in all
   conducted assays and it was the most effective one against all tested
   cell lines, presenting GI(50) values of 7.02-52.69 mu g/mL. Moreover,
   none of the EOs showed toxicity at tested concentrations (>400 mu g/mL)
   for porcine liver primary cell culture. In addition to their well-known
   traditional use in food and cosmetics, the great potential of tested
   Thymus essential oils for application in oral diseases and anticancer
   treatments, encourage further investigation. (C) 2013 Elsevier B.V. All
   rights reserved.",
journal = "Industrial Crops and Products",
title = "Chemical composition, antimicrobial, antioxidant and antitumor activity
 of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus
 vulgaris L. essential oils",
volume = "52",
doi = "10.1016/j.indcrop.2013.10.006",
pages = "183-190"
}

Nikolić, M., Glamočlija, J., Ferreira, I. C. F. R., Calhelha, R. C., Fernandes, A., Markovic, T., Markovic, D., Giweli, A.,& Soković, M.. (2014). Chemical composition, antimicrobial, antioxidant and antitumor activity
 of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus
 vulgaris L. essential oils. in Industrial Crops and Products, 52, 183-190.
https://doi.org/10.1016/j.indcrop.2013.10.006

Nikolić M, Glamočlija J, Ferreira ICFR, Calhelha RC, Fernandes A, Markovic T, Markovic D, Giweli A, Soković M. Chemical composition, antimicrobial, antioxidant and antitumor activity
 of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus
 vulgaris L. essential oils. in Industrial Crops and Products. 2014;52:183-190.
doi:10.1016/j.indcrop.2013.10.006 .

Nikolić, Miloš, Glamočlija, Jasmina, Ferreira, Isabel C. F. R., Calhelha, Ricardo C., Fernandes, Angela, Markovic, Tatjana, Markovic, Dejan, Giweli, Abdulhamed, Soković, Marina, "Chemical composition, antimicrobial, antioxidant and antitumor activity
 of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus
 vulgaris L. essential oils" in Industrial Crops and Products, 52 (2014):183-190,
https://doi.org/10.1016/j.indcrop.2013.10.006 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Stojković, Dejan
AU  - Nikolić, Miloš
AU  - Ćirić, Ana
AU  - Fernandes, Angela
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2267
AB  - Agrocybe aegerita (Brig.) Sing is a basidiomycete, white rot fungus.
   Antimicrobial activities and the antiqourum effect on Pseudomonas
   aeruginosa of an A. aegerita methanolic extract were investigated. The
   extract showed very good antimicrobial activity against all the tested
   microorganisms in a dose dependent manner. Effects of the Sub-MIC, MIC
   and 2MIC of the A. aegerita methanolic extract regulated the virulence
   factors in the quorum sensing (QS) test, as well as biofilm formation on
   P. aeruginosa. Sub-inhibitory and inhibitory concentrations of the
   extract demonstrated the reduction of virulence factors such as
   pyocyanin production, twitching and swimming motility. The biofilm
   forming capability of P. aeruginosa PAO1 was also reduced in a
   concentration-dependent manner. Furthermore, the chemical composition of
   the methanolic extract was determined considering its phenolic
   composition. The methanolic extract of A. aegerita can be a very good
   source of bioactive substances. This research is of great importance due
   to the prevalence of drug-resistant microorganisms.
T2  - Food & Function
T1  - Bioactive composition, antimicrobial activities and the influence of
 Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated
 functions and biofilm formation by Pseudomonas aeruginosa
IS  - 12
VL  - 5
DO  - 10.1039/c4fo00819g
SP  - 3296
EP  - 3303
ER  - 

@article{
author = "Petrović, Jovana and Glamočlija, Jasmina and Stojković, Dejan and Nikolić, Miloš and Ćirić, Ana and Fernandes, Angela and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2014",
abstract = "Agrocybe aegerita (Brig.) Sing is a basidiomycete, white rot fungus.
   Antimicrobial activities and the antiqourum effect on Pseudomonas
   aeruginosa of an A. aegerita methanolic extract were investigated. The
   extract showed very good antimicrobial activity against all the tested
   microorganisms in a dose dependent manner. Effects of the Sub-MIC, MIC
   and 2MIC of the A. aegerita methanolic extract regulated the virulence
   factors in the quorum sensing (QS) test, as well as biofilm formation on
   P. aeruginosa. Sub-inhibitory and inhibitory concentrations of the
   extract demonstrated the reduction of virulence factors such as
   pyocyanin production, twitching and swimming motility. The biofilm
   forming capability of P. aeruginosa PAO1 was also reduced in a
   concentration-dependent manner. Furthermore, the chemical composition of
   the methanolic extract was determined considering its phenolic
   composition. The methanolic extract of A. aegerita can be a very good
   source of bioactive substances. This research is of great importance due
   to the prevalence of drug-resistant microorganisms.",
journal = "Food & Function",
title = "Bioactive composition, antimicrobial activities and the influence of
 Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated
 functions and biofilm formation by Pseudomonas aeruginosa",
number = "12",
volume = "5",
doi = "10.1039/c4fo00819g",
pages = "3296-3303"
}

Petrović, J., Glamočlija, J., Stojković, D., Nikolić, M., Ćirić, A., Fernandes, A., Ferreira, I. C. F. R.,& Soković, M.. (2014). Bioactive composition, antimicrobial activities and the influence of
 Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated
 functions and biofilm formation by Pseudomonas aeruginosa. in Food & Function, 5(12), 3296-3303.
https://doi.org/10.1039/c4fo00819g

Petrović J, Glamočlija J, Stojković D, Nikolić M, Ćirić A, Fernandes A, Ferreira ICFR, Soković M. Bioactive composition, antimicrobial activities and the influence of
 Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated
 functions and biofilm formation by Pseudomonas aeruginosa. in Food & Function. 2014;5(12):3296-3303.
doi:10.1039/c4fo00819g .

Petrović, Jovana, Glamočlija, Jasmina, Stojković, Dejan, Nikolić, Miloš, Ćirić, Ana, Fernandes, Angela, Ferreira, Isabel C. F. R., Soković, Marina, "Bioactive composition, antimicrobial activities and the influence of
 Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated
 functions and biofilm formation by Pseudomonas aeruginosa" in Food & Function, 5, no. 12 (2014):3296-3303,
https://doi.org/10.1039/c4fo00819g . .