TY  - JOUR
AU  - Todorović, Lidija
AU  - Stamenković, Gorana
AU  - Vučetić-Tadić, Biljana
AU  - Umezawa, Kazuo
AU  - Božović, Ana
AU  - Yamashita, Shunichi
AU  - Stanojević, Boban
PY  - 2021
UR  - https://doi.org/10.2298/ABS201010055T
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6068
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4199
AB  - The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2
IS  - 1
VL  - 73
DO  - 10.2298/abs201010055t
SP  - 31
EP  - 38
ER  - 

@article{
author = "Todorović, Lidija and Stamenković, Gorana and Vučetić-Tadić, Biljana and Umezawa, Kazuo and Božović, Ana and Yamashita, Shunichi and Stanojević, Boban",
year = "2021",
abstract = "The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2",
number = "1",
volume = "73",
doi = "10.2298/abs201010055t",
pages = "31-38"
}

Todorović, L., Stamenković, G., Vučetić-Tadić, B., Umezawa, K., Božović, A., Yamashita, S.,& Stanojević, B.. (2021). Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2. in Archives of Biological Sciences
Serbian Biological Society., 73(1), 31-38.
https://doi.org/10.2298/abs201010055t

Todorović L, Stamenković G, Vučetić-Tadić B, Umezawa K, Božović A, Yamashita S, Stanojević B. Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2. in Archives of Biological Sciences. 2021;73(1):31-38.
doi:10.2298/abs201010055t .

Todorović, Lidija, Stamenković, Gorana, Vučetić-Tadić, Biljana, Umezawa, Kazuo, Božović, Ana, Yamashita, Shunichi, Stanojević, Boban, "Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2" in Archives of Biological Sciences, 73, no. 1 (2021):31-38,
https://doi.org/10.2298/abs201010055t . .

TY  - JOUR
AU  - Stanojević, Boban R
AU  - Osiowy, Carla
AU  - Schaefer, Stephan
AU  - Bojović, Ksenija
AU  - Blagojević, Jelena
AU  - Nesić, Milica
AU  - Yamashita, Shunichi
AU  - Stamenković, Gorana
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1274
AB  - Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,
T2  - Infection Genetics and Evolution
T1  - Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia
IS  - 6
VL  - 11
EP  - 1480
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1274
ER  - 

@article{
author = "Stanojević, Boban R and Osiowy, Carla and Schaefer, Stephan and Bojović, Ksenija and Blagojević, Jelena and Nesić, Milica and Yamashita, Shunichi and Stamenković, Gorana",
year = "2011",
abstract = "Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,",
journal = "Infection Genetics and Evolution",
title = "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia",
number = "6",
volume = "11",
pages = "1480",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1274"
}

Stanojević, B. R., Osiowy, C., Schaefer, S., Bojović, K., Blagojević, J., Nesić, M., Yamashita, S.,& Stamenković, G.. (2011). Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution, 11(6).
https://hdl.handle.net/21.15107/rcub_ibiss_1274

Stanojević BR, Osiowy C, Schaefer S, Bojović K, Blagojević J, Nesić M, Yamashita S, Stamenković G. Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution. 2011;11(6):null-1480.
https://hdl.handle.net/21.15107/rcub_ibiss_1274 .

Stanojević, Boban R, Osiowy, Carla, Schaefer, Stephan, Bojović, Ksenija, Blagojević, Jelena, Nesić, Milica, Yamashita, Shunichi, Stamenković, Gorana, "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia" in Infection Genetics and Evolution, 11, no. 6 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1274 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Stanojević, Boban R
AU  - Tanić, Nasta
AU  - Schaefer, Stephan
AU  - Niesters, Hubert GM
AU  - Bozić, Milena
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1424
AB  - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Journal of Virological Methods
T1  - Concurrent quantitation of the A and D genotypes of hepatitis B virus
IS  - 2
VL  - 161
EP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1424
ER  - 

@article{
author = "Tanić, Nikola T and Stanojević, Boban R and Tanić, Nasta and Schaefer, Stephan and Niesters, Hubert GM and Bozić, Milena and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Journal of Virological Methods",
title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus",
number = "2",
volume = "161",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1424"
}

Tanić, N. T., Stanojević, B. R., Tanić, N., Schaefer, S., Niesters, H. G., Bozić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1424

Tanić NT, Stanojević BR, Tanić N, Schaefer S, Niesters HG, Bozić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):null-270.
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .

Tanić, Nikola T, Stanojević, Boban R, Tanić, Nasta, Schaefer, Stephan, Niesters, Hubert GM, Bozić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .