TY  - JOUR
AU  - Marković, Snežana D.
AU  - Đorđević, Nataša Z.
AU  - Ćurčić, Milena G.
AU  - Štajn, Andraš Š.
AU  - Spasić, Mihajlo
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2151
AB  - The effects of nitroglycerin (glyceryl trinitrate - GTN) are mediated by
   liberated nitric oxide (NO) and formed reactive nitrogen species, which
   induces oxidative stress during biotransformation in red blood cells
   (RBCs). The aim of this study was to evaluate effects of GTN on
   antioxidative defense system (AOS) in rat erythrocytes (without) and
   reticulocytes (with functional mitochondria). Rat erythrocyte and
   reticulocyte-rich RBC suspensions were aerobically incubated (2 h, 37
   degrees C) without (control) or in the presence of different
   concentrations of GTN (0.1-1.5 mM). After incubation, concentrations of
   non-enzymatic components of AOS, activities of antioxidative enzymes and
   oxidative pentose phosphate (OPP) pathway activity were followed in RBC
   suspensions. In rat reticulocytes, GTN decreased the activity of
   mitochondrial MnSOD and increased the activity of CuZnSOD. In rat RBCs,
   GTN induced increase of Vit E concentration (at high doses), but
   decreased glutathione content and activities of all
   glutathione-dependent antioxidative enzymes; the OPP pathway activity
   significantly increased. GTN biotransformation and induction of
   oxidative stress were followed by general disbalance of antioxidative
   capacities in both kinds of RBCs. We suggest that oxidative stress,
   MnSOD inhibition and depletion of glutathione pool in response to GTN
   treatment lead to decreased bioavailability of NO after GTN
   biotransformation in rat reticulocytes.
T2  - General Physiology and Biophysics
T1  - Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes
IS  - 4
VL  - 33
DO  - 10.4149/gpb_2014018
SP  - 393
EP  - 401
ER  - 

@article{
author = "Marković, Snežana D. and Đorđević, Nataša Z. and Ćurčić, Milena G. and Štajn, Andraš Š. and Spasić, Mihajlo",
year = "2014",
abstract = "The effects of nitroglycerin (glyceryl trinitrate - GTN) are mediated by
   liberated nitric oxide (NO) and formed reactive nitrogen species, which
   induces oxidative stress during biotransformation in red blood cells
   (RBCs). The aim of this study was to evaluate effects of GTN on
   antioxidative defense system (AOS) in rat erythrocytes (without) and
   reticulocytes (with functional mitochondria). Rat erythrocyte and
   reticulocyte-rich RBC suspensions were aerobically incubated (2 h, 37
   degrees C) without (control) or in the presence of different
   concentrations of GTN (0.1-1.5 mM). After incubation, concentrations of
   non-enzymatic components of AOS, activities of antioxidative enzymes and
   oxidative pentose phosphate (OPP) pathway activity were followed in RBC
   suspensions. In rat reticulocytes, GTN decreased the activity of
   mitochondrial MnSOD and increased the activity of CuZnSOD. In rat RBCs,
   GTN induced increase of Vit E concentration (at high doses), but
   decreased glutathione content and activities of all
   glutathione-dependent antioxidative enzymes; the OPP pathway activity
   significantly increased. GTN biotransformation and induction of
   oxidative stress were followed by general disbalance of antioxidative
   capacities in both kinds of RBCs. We suggest that oxidative stress,
   MnSOD inhibition and depletion of glutathione pool in response to GTN
   treatment lead to decreased bioavailability of NO after GTN
   biotransformation in rat reticulocytes.",
journal = "General Physiology and Biophysics",
title = "Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes",
number = "4",
volume = "33",
doi = "10.4149/gpb_2014018",
pages = "393-401"
}

Marković, S. D., Đorđević, N. Z., Ćurčić, M. G., Štajn, A. Š.,& Spasić, M.. (2014). Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes. in General Physiology and Biophysics, 33(4), 393-401.
https://doi.org/10.4149/gpb_2014018

Marković SD, Đorđević NZ, Ćurčić MG, Štajn AŠ, Spasić M. Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes. in General Physiology and Biophysics. 2014;33(4):393-401.
doi:10.4149/gpb_2014018 .

Marković, Snežana D., Đorđević, Nataša Z., Ćurčić, Milena G., Štajn, Andraš Š., Spasić, Mihajlo, "Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes" in General Physiology and Biophysics, 33, no. 4 (2014):393-401,
https://doi.org/10.4149/gpb_2014018 . .

TY  - CONF
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Đorđević, Nataša
AU  - Pavlović, Slađan
AU  - Štajn, Andraš Š.
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5999
AB  - The environment is continuously loaded with foreign organic chemicals (xenobiotics) released by urban communities and industries. Many classes of environmental contaminants (or their metabolites) are know to enhance the intracellular formation of reactive oxygen species (ROS) causing oxidative damage to biological systems. Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment.
Specimens two marine fishes of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) were collected May 2005 from the locality in front of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress: activity of antioxidative enzymes (superoxide dismutase­ SOD and catalase-CAT) and concentration of lipid peroxidation-LP and glutathione-GSH were determined in the liver of hake and red mullet.
Obtained results showed that activities of SOD and CAT were significantly lower in the liver of hake than in red mullet. Higher concentrations of LP and GSH in liver of hake were recorded, due to changes ofphysical­ chemical parameters of environment. The intensity of oxidative stress in liver of hake was higher in comparison to red mullet. This increased ROS production and subsequent oxidati e damage has been associated with pollutant mediated mechanism of toxicity in liver of investigated fishes.
According to the analyses of our results the investigated parameters of oxidative stress (concentration of LP and GSH, as well as activity of antioxidative enzymes SOD and CAT) in liver of hake and red mullet demonstrate     significant     and     reliable     biomarkers     m  monitoring,environmental  pollution.
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5999
ER  - 

@conference{
author = "Ognjanović, Branka and Marković, Snežana and Đorđević, Nataša and Pavlović, Slađan and Štajn, Andraš Š. and Žikić, Radoslav V. and Saičić, Zorica",
year = "2007",
abstract = "The environment is continuously loaded with foreign organic chemicals (xenobiotics) released by urban communities and industries. Many classes of environmental contaminants (or their metabolites) are know to enhance the intracellular formation of reactive oxygen species (ROS) causing oxidative damage to biological systems. Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment.
Specimens two marine fishes of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) were collected May 2005 from the locality in front of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress: activity of antioxidative enzymes (superoxide dismutase­ SOD and catalase-CAT) and concentration of lipid peroxidation-LP and glutathione-GSH were determined in the liver of hake and red mullet.
Obtained results showed that activities of SOD and CAT were significantly lower in the liver of hake than in red mullet. Higher concentrations of LP and GSH in liver of hake were recorded, due to changes ofphysical­ chemical parameters of environment. The intensity of oxidative stress in liver of hake was higher in comparison to red mullet. This increased ROS production and subsequent oxidati e damage has been associated with pollutant mediated mechanism of toxicity in liver of investigated fishes.
According to the analyses of our results the investigated parameters of oxidative stress (concentration of LP and GSH, as well as activity of antioxidative enzymes SOD and CAT) in liver of hake and red mullet demonstrate     significant     and     reliable     biomarkers     m  monitoring,environmental  pollution.",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5999"
}

Ognjanović, B., Marković, S., Đorđević, N., Pavlović, S., Štajn, A. Š., Žikić, R. V.,& Saičić, Z.. (2007). Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5999

Ognjanović B, Marković S, Đorđević N, Pavlović S, Štajn AŠ, Žikić RV, Saičić Z. Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5999 .

Ognjanović, Branka, Marković, Snežana, Đorđević, Nataša, Pavlović, Slađan, Štajn, Andraš Š., Žikić, Radoslav V., Saičić, Zorica, "Oxidative stress parameters in the liver of hake (Merluccius merluccius L.) and red mullet (Mullus barbatus L.) from the Adriatic sea" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5999 .

TY  - CONF
AU  - Đorđević, Nataša
AU  - Ognjanović, Branka
AU  - Marković, Snežana
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Štajn, Andraš Š.
AU  - Saičić, Zorica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5998
AB  - Recent investigations in biomonitoring include the monitoring of biochemical and physiological parameters to evaluate the condition of environment. Parameters of oxidative stress are significant biomarkers of environmental pollution of the sea. Specimens of marine fish - hake (Merluccius merluccius) was collected from the localities of Platamuni and Valdanos (Sauth Adriatic) in winter   and   spring   during   2003   year.   Concentrations   of antioxidant compounds such as Vitamin E (Vit E) and Vitamin C (Vit C) as well as, lipid peroxidation (LP) were determined in the liver and white muscle of the hake. Physical chemical parameters of water of investigated  localities  were also determined: salinity, temperature, oxygen saturation and concentrations of nitrites, nitrates and detergent in both seasons.
The obtained results showed higher concentrations of LP, Vit E and Vit C in liver of hake in comparison to white muscle. Concentrations of LP and Vit C were increased, while the concentrations of Vit E were decreased in winter period in comparison to spring. Concentrations of LP were increased while the concentrations of Vit E were decreased in both investigated tissues of hake from the waters of locality Valdanos in respect to Platamuni. These changes of parameters of oxidative stress are, in part, the consequence of changes of temperature as well as, concentrations of nitrites, nitrates and detergents in water of investigated localities and in particular season.
In conclusion, the concentrations of parameter of oxidative stress in investigated tissues of hake represent a good marker in the biomonitoring of environmental pollution. The intensity of oxidative stress was increased in the liver of hake in comparison to white muscle, in winter period ill respect to spring and in waters of Valdanos in comparison to Platamuni
PB  - Novi Sad: Faculty of Sciences, Department of Biology and Ecology
C3  - Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
T1  - Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5998
ER  - 

@conference{
author = "Đorđević, Nataša and Ognjanović, Branka and Marković, Snežana and Pavlović, Slađan and Žikić, Radoslav V. and Štajn, Andraš Š. and Saičić, Zorica",
year = "2007",
abstract = "Recent investigations in biomonitoring include the monitoring of biochemical and physiological parameters to evaluate the condition of environment. Parameters of oxidative stress are significant biomarkers of environmental pollution of the sea. Specimens of marine fish - hake (Merluccius merluccius) was collected from the localities of Platamuni and Valdanos (Sauth Adriatic) in winter   and   spring   during   2003   year.   Concentrations   of antioxidant compounds such as Vitamin E (Vit E) and Vitamin C (Vit C) as well as, lipid peroxidation (LP) were determined in the liver and white muscle of the hake. Physical chemical parameters of water of investigated  localities  were also determined: salinity, temperature, oxygen saturation and concentrations of nitrites, nitrates and detergent in both seasons.
The obtained results showed higher concentrations of LP, Vit E and Vit C in liver of hake in comparison to white muscle. Concentrations of LP and Vit C were increased, while the concentrations of Vit E were decreased in winter period in comparison to spring. Concentrations of LP were increased while the concentrations of Vit E were decreased in both investigated tissues of hake from the waters of locality Valdanos in respect to Platamuni. These changes of parameters of oxidative stress are, in part, the consequence of changes of temperature as well as, concentrations of nitrites, nitrates and detergents in water of investigated localities and in particular season.
In conclusion, the concentrations of parameter of oxidative stress in investigated tissues of hake represent a good marker in the biomonitoring of environmental pollution. The intensity of oxidative stress was increased in the liver of hake in comparison to white muscle, in winter period ill respect to spring and in waters of Valdanos in comparison to Platamuni",
publisher = "Novi Sad: Faculty of Sciences, Department of Biology and Ecology",
journal = "Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia",
title = "Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5998"
}

Đorđević, N., Ognjanović, B., Marković, S., Pavlović, S., Žikić, R. V., Štajn, A. Š.,& Saičić, Z.. (2007). Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia
Novi Sad: Faculty of Sciences, Department of Biology and Ecology., 55.
https://hdl.handle.net/21.15107/rcub_ibiss_5998

Đorđević N, Ognjanović B, Marković S, Pavlović S, Žikić RV, Štajn AŠ, Saičić Z. Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea. in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia. 2007;:55.
https://hdl.handle.net/21.15107/rcub_ibiss_5998 .

Đorđević, Nataša, Ognjanović, Branka, Marković, Snežana, Pavlović, Slađan, Žikić, Radoslav V., Štajn, Andraš Š., Saičić, Zorica, "Concentration of antioxidant compounds and lipid peroxidation in some tissues of hake (Merluccius merluccius L.) from the Adriatic sea" in Collection of abstracts: 1st International Congress on Biology in Serbia: Kobis 2007; 2007 Oct 25-28; Palić, Serbia (2007):55,
https://hdl.handle.net/21.15107/rcub_ibiss_5998 .