TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6592
AB  - Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6592
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6592"
}

Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kolarević, S.. (2023). In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592

Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kolarević S. In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kolarević, Stoimir, "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac024/6823671
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5243
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac024
SP  - 21
EP  - 32
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024",
pages = "21-32"
}

Jovanović Marić, J., Kolarević, S., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford: Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Oswald, Petar
AU  - Oswaldova, Martina
AU  - Slobodnik, Jaroslav
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kološa, Katja
AU  - Žegura, Bojana
AU  - Kolarević, Stoimir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5725
AB  - Within the Joint Danube Survey 4 (JDS4) (2019) surface water samples
were collected at 24 localities along the Danube River and were extracted by
horizon large volume solid-phase extraction (LVSPE) technique. LVSPE
technique provided 25,000x relative enrichment factor (REF) of the river
samples. The bioactivity of the LVSPE samples was investigated employing
prokaryotic SOS/umuC assay (S9+ and S9-) and battery of bioassays
analyzing different endpoints – cytotoxicity (MTS assay), genotoxicity
(comet assay), cell cycle analyses of ZFL cells and embryo toxicity (zFET) in
eukaryotic models (zebrafish liver cell line – ZFL and Danio rerio embryos –
zebrafish). In the case of the SOS/umuC assay none of the tested samples
(REF100) has induced genotoxic effect. The cytotoxic effect was detected for
11 samples at REF100 (MTS assay). The comet assay results indicated
genotoxic potential for nine samples – one in the Upper Danube, six in the
Middle Danube and two in the Lower Danube. The most potent were
JDS4-37 and JDS4-41 samples collected at two sites in the Serbian part of
the Danube River. These samples were analyzed at REF13. The analysis of
the cell cycle was performed in the case of eight samples for which were
previously recorded genotoxic effect. For one sample (JDS4-41) was found
the G1 phase cell cycle arrest. The zFET assay was performed for all the
samples at REF100 and there was no embryo toxicity or teratogenicity
recorded. The results obtained within this study indicate a higher sensitivity
of eukaryotic models compared to prokaryotic SOS/umuC assay. Likewise, it
was found that most of the genotoxic samples originate from the middle
part of the Danube.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5725
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Oswald, Petar and Oswaldova, Martina and Slobodnik, Jaroslav and Vuković-Gačić, Branka and Paunović, Momir and Kološa, Katja and Žegura, Bojana and Kolarević, Stoimir",
year = "2022",
abstract = "Within the Joint Danube Survey 4 (JDS4) (2019) surface water samples
were collected at 24 localities along the Danube River and were extracted by
horizon large volume solid-phase extraction (LVSPE) technique. LVSPE
technique provided 25,000x relative enrichment factor (REF) of the river
samples. The bioactivity of the LVSPE samples was investigated employing
prokaryotic SOS/umuC assay (S9+ and S9-) and battery of bioassays
analyzing different endpoints – cytotoxicity (MTS assay), genotoxicity
(comet assay), cell cycle analyses of ZFL cells and embryo toxicity (zFET) in
eukaryotic models (zebrafish liver cell line – ZFL and Danio rerio embryos –
zebrafish). In the case of the SOS/umuC assay none of the tested samples
(REF100) has induced genotoxic effect. The cytotoxic effect was detected for
11 samples at REF100 (MTS assay). The comet assay results indicated
genotoxic potential for nine samples – one in the Upper Danube, six in the
Middle Danube and two in the Lower Danube. The most potent were
JDS4-37 and JDS4-41 samples collected at two sites in the Serbian part of
the Danube River. These samples were analyzed at REF13. The analysis of
the cell cycle was performed in the case of eight samples for which were
previously recorded genotoxic effect. For one sample (JDS4-41) was found
the G1 phase cell cycle arrest. The zFET assay was performed for all the
samples at REF100 and there was no embryo toxicity or teratogenicity
recorded. The results obtained within this study indicate a higher sensitivity
of eukaryotic models compared to prokaryotic SOS/umuC assay. Likewise, it
was found that most of the genotoxic samples originate from the middle
part of the Danube.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5725"
}

Kračun-Kolarević, M., Jovanović Marić, J., Oswald, P., Oswaldova, M., Slobodnik, J., Vuković-Gačić, B., Paunović, M., Kološa, K., Žegura, B.,& Kolarević, S.. (2022). Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5725

Kračun-Kolarević M, Jovanović Marić J, Oswald P, Oswaldova M, Slobodnik J, Vuković-Gačić B, Paunović M, Kološa K, Žegura B, Kolarević S. Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5725 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Oswald, Petar, Oswaldova, Martina, Slobodnik, Jaroslav, Vuković-Gačić, Branka, Paunović, Momir, Kološa, Katja, Žegura, Bojana, Kolarević, Stoimir, "Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5725 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
AU  - Žegura, Bojana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6233
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays
SP  - 160
EP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6233
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Oswald, Peter and Slobodnik, Jaroslav and Paunović, Momir and Vuković-Gačić, Branka and Žegura, Bojana",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays",
pages = "160-161",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6233"
}

Kolarević, S., Kračun-Kolarević, M., Oswald, P., Slobodnik, J., Paunović, M., Vuković-Gačić, B.,& Žegura, B.. (2022). Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 160-161.
https://hdl.handle.net/21.15107/rcub_ibiss_6233

Kolarević S, Kračun-Kolarević M, Oswald P, Slobodnik J, Paunović M, Vuković-Gačić B, Žegura B. Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:160-161.
https://hdl.handle.net/21.15107/rcub_ibiss_6233 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Oswald, Peter, Slobodnik, Jaroslav, Paunović, Momir, Vuković-Gačić, Branka, Žegura, Bojana, "Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):160-161,
https://hdl.handle.net/21.15107/rcub_ibiss_6233 .

TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milošković, Aleksandra
AU  - Radojković, Nataša
AU  - Simić, Vladica
AU  - Dojčinović, Biljana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Timilijić, Jovana
AU  - Djordjević, Jelena
AU  - Žegura, Bojana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S004896971732805X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2887
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
T2  - Science of The Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
SP  - 1289
EP  - 1299
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Radojković, Nataša and Simić, Vladica and Dojčinović, Biljana and Kračun-Kolarević, Margareta and Paunović, Momir and Kostić, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka and Timilijić, Jovana and Djordjević, Jelena and Žegura, Bojana",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
journal = "Science of The Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099",
pages = "1289-1299"
}

Jovanović, J., Kolarević, S., Milošković, A., Radojković, N., Simić, V., Dojčinović, B., Kračun-Kolarević, M., Paunović, M., Kostić, J., Sunjog, K., Gačić, Z., Vuković-Gačić, B., Timilijić, J., Djordjević, J.,& Žegura, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of The Total Environment, 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milošković A, Radojković N, Simić V, Dojčinović B, Kračun-Kolarević M, Paunović M, Kostić J, Sunjog K, Gačić Z, Vuković-Gačić B, Timilijić J, Djordjević J, Žegura B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of The Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Radojković, Nataša, Simić, Vladica, Dojčinović, Biljana, Kračun-Kolarević, Margareta, Paunović, Momir, Kostić, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, Timilijić, Jovana, Djordjević, Jelena, Žegura, Bojana, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of The Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .