@article{
author = "Šošić-Jurjević, Branka and Filipović, Branko and Renko, Kostja and Miler, Marko and Trifunović, Svetlana and Ajdžanović, Vladimir and Koehrle, Josef and Milošević, Verica",
year = "2015",
abstract = "We previously reported that orchidectomy (Orx) of middle-aged rats
(15-16-month-old; MA) slightly affected pituitary-thyroid axis, but
decreased liver deiodinase (Dio) type I and pituitary Dio2 enzyme
activities. At present, we examined the effects of subsequent
testosterone-propionate treatment (5 mg/kg; Orx + T), and compared the
effects of testosterone with the effects of estradiol-clipropionate
(0.06 mg/kg; Orx + E) treatment. Hormones were subcutaneously
administered, daily, for three weeks, while Orx and sham-operated (SO)
controls received only the vehicle. The applied dose of T did not alter
serum TSH, T4 and T3 concentrations in Orx- MA, though it increased TSH
when administrated to Orx young adults (2.5-month-old; Orx-YA). However,
pituitaries of Orx-MA + T rats had higher relative intensity of
immunofluorescence (RIP) for TSHI3; in their thyroids we found increased
volume and height of follicular epithelium, decreased volume of the
colloid and higher RIF for T,{[}-bound to thyroglobulin (Tg-T4). Liver
Diol activity was increased. E-treatment did not affect serum hormone
levels, pituitary RIF for TS1-11i, or liver Diol activity in Orx-MA
rats. Thyroids had decreased relative volume and height of follicular
epithelium, increased relative volume of the colloid, decreased volume
of sodium iodide symporter-immunopositive epithelium and lower RIF for
Tg-T4. Detected changes were statistically significant. In conclusion,
androgenization enhanced pituitary TSHr, RIF, thyroid activation and
liver Diol enzyme activity in Orx-MA, without elevating serum TSH as in
Orx-YA rats. Estrogenization induced pituitary enlargement with no
effect on pituitary TSHI) RIF, serum TSH or liver Diol activity. E also
induced alterations in thyroid histology that indicate mild suppression
of its functioning, and contributed to thyroid blood vessel enlargement
in Orx-MA rats. (C) 2015 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Experimental Gerontology",
title = "Testosterone and estradiol treatments differently affect
pituitary-thyroid axis and liver deiodinase 1 activity in
orchidectomized middle-aged rats",
volume = "72",
doi = "10.1016/j.exger.2015.09.010",
pages = "85-98"
}