TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Đokić, Marko
AU  - Stamenković, Gorana
AU  - Barišić Klisarić, Nataša
AU  - Stojković, Oliver
AU  - Jojić, Vida
AU  - Savić, Ivo
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6587
AB  - Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed.
PB  - Basel: MDPI
T2  - Animals
T1  - A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct
IS  - 5
VL  - 14
DO  - 10.3390/ani14050774
SP  - 774
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Đokić, Marko and Stamenković, Gorana and Barišić Klisarić, Nataša and Stojković, Oliver and Jojić, Vida and Savić, Ivo",
year = "2024",
abstract = "Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct",
number = "5",
volume = "14",
doi = "10.3390/ani14050774",
pages = "774"
}

Bugarski-Stanojević, V., Đokić, M., Stamenković, G., Barišić Klisarić, N., Stojković, O., Jojić, V.,& Savić, I.. (2024). A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct. in Animals
Basel: MDPI., 14(5), 774.
https://doi.org/10.3390/ani14050774

Bugarski-Stanojević V, Đokić M, Stamenković G, Barišić Klisarić N, Stojković O, Jojić V, Savić I. A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct. in Animals. 2024;14(5):774.
doi:10.3390/ani14050774 .

Bugarski-Stanojević, Vanja, Đokić, Marko, Stamenković, Gorana, Barišić Klisarić, Nataša, Stojković, Oliver, Jojić, Vida, Savić, Ivo, "A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct" in Animals, 14, no. 5 (2024):774,
https://doi.org/10.3390/ani14050774 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Savić, Ivo
PY  - 2022
UR  - https://www.mdpi.com/2076-2615/12/9/1097
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9105853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4964
AB  - We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs-the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher 2n are evolutionary older corresponds to the fusion hypothesis of Nannospalax karyotype evolution. To participate in conservation strategies, N. leucodon classification should follow the biological species concept, and proposed cryptic species should be formally named, despite a lack of classical morphometric discrepancy. We draw attention towards the syrmiensis and montanosyrmiensis CFs, estimated to be endangered/critically endangered, and emphasise the need for detailed monitoring and population survey for other cryptic species.
PB  - Basel: MDPI
T2  - Animals
T1  - Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.
IS  - 9
VL  - 12
DO  - 10.3390/ani12091097
SP  - 1097
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Jojić, Vida and Ćosić, Nada and Ćirović, Duško and Stojković, Oliver and Veličković, Jelena and Savić, Ivo",
year = "2022",
abstract = "We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs-the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher 2n are evolutionary older corresponds to the fusion hypothesis of Nannospalax karyotype evolution. To participate in conservation strategies, N. leucodon classification should follow the biological species concept, and proposed cryptic species should be formally named, despite a lack of classical morphometric discrepancy. We draw attention towards the syrmiensis and montanosyrmiensis CFs, estimated to be endangered/critically endangered, and emphasise the need for detailed monitoring and population survey for other cryptic species.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.",
number = "9",
volume = "12",
doi = "10.3390/ani12091097",
pages = "1097"
}

Bugarski-Stanojević, V., Stamenković, G., Jojić, V., Ćosić, N., Ćirović, D., Stojković, O., Veličković, J.,& Savić, I.. (2022). Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.. in Animals
Basel: MDPI., 12(9), 1097.
https://doi.org/10.3390/ani12091097

Bugarski-Stanojević V, Stamenković G, Jojić V, Ćosić N, Ćirović D, Stojković O, Veličković J, Savić I. Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.. in Animals. 2022;12(9):1097.
doi:10.3390/ani12091097 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Jojić, Vida, Ćosić, Nada, Ćirović, Duško, Stojković, Oliver, Veličković, Jelena, Savić, Ivo, "Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation." in Animals, 12, no. 9 (2022):1097,
https://doi.org/10.3390/ani12091097 . .

TY  - CONF
AU  - Đokić, Marko
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Savić, Ivo
AU  - Bugarski-Stanojević, Vanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5312
AB  - Vrste slepog kučeta iz roda Nannospalax se, pored brojnih adaptacija na podzemni način života, karakterišu i izraženom varijabilnošću kariotipa. Do sada su opisane čak 74 različite hromozomske forme (HF), ali su zvanično priznate samo tri vrste.1 Svaka je zapravo kompleks vrsta sa statusom „nedovoljno podataka“ na IUCN Red List of Threatened Species. Evropske vrste ovog roda sačinjavaju kompleks N. leucodon supersp.1 Među njima je više od 20 različitih HF, od kojih je pet (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) prisutno u Srbiji, a poslednje dve se vode kao ugrožene.2 Iako su međusobno reproduktivno izolovane i filogenetski udaljene, zbog nedostatka morfoloških razlika predstavljaju kriptične vrste. Sa konzervacionog aspekta je veoma važno vršiti monitoring njihovih populacija i utvrditi areale rasprostanjenja. Stoga je neophodno razviti pouzdanu i neinvazivnu metodu za njihovu efikasnu determinaciju. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) tehnika, koja produkuje ISSR markere – anonimne sekvence DNK ograničene invertovanim tandemskim ponovcima, se pokazala kao korisna u identifikaciji morfološki sličnih vrsta kod nekoliko sisarskih taksona.3 Od 11 različitih ISSR prajmera testiranih na prisustvo fragmenata DNK specifičnih za vrstu, na ukupnom uzorku od 25 jedinki, tri prajmera (CAA)5GC; (CAG)4AC i (GA)8AC su dala informativne profile DNK, obezbeđujući pouzdano i jasno prepoznavanje svih pet analiziranih kriptičnih vrsta.
AB  - Врсте слепог кучета из рода Nannospalax се, поред бројних адаптација на подземни начин живота, карактеришу и израженом варијабилношћу кариотипа. До сада су описане чак 74 различите хромозомске форме (ХФ), али су званично признате само три врсте.1 Свака је заправо комплекс врста са статусом „недовољно података“ на IUCN Red List of Threatened Species. Европске врсте овог рода сачињавају комплекс N. leucodon supersp.1 Међу њима је више од 20 различитих ХФ, од којих је пет (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) присутно у Србији, а последње две се воде као угрожене.2 Иако су међусобно репродуктивно изоловане и филогенетски удаљене, због недостатка морфолошких разлика представљају криптичне врсте. Са конзервационог аспекта је веома важно вршити мониторинг њихових популација и утврдити ареале распростањења. Стога је неопходно развити поуздану и неинвазивну методу за њихову ефикасну детерминацију. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) техника, која продукује ISSR маркере – анонимне секвенце ДНК ограничене инвертованим тандемским поновцима, се показала као корисна у идентификацији морфолошки сличних врста код неколико сисарских таксона.3 Од 11 различитих ISSR прајмера тестираних на присуство фрагмената ДНК специфичних за врсту, на укупном узорку од 25 јединки, три прајмера (CAA)5GC; (CAG)4AC и (GA)8AC су дала информативне профиле ДНК, обезбеђујући поуздано и јасно препознавање свих пет анализираних криптичних врста.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia)
T1  - ISSR-PCR као једноставна дијагностичка метода за разликовање криптичних врста Nannospalax leucodon (Spalacinae, Rodentia)
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5312
ER  - 

@conference{
author = "Đokić, Marko and Stamenković, Gorana and Jojić, Vida and Ćosić, Nada and Ćirović, Duško and Savić, Ivo and Bugarski-Stanojević, Vanja",
year = "2022",
abstract = "Vrste slepog kučeta iz roda Nannospalax se, pored brojnih adaptacija na podzemni način života, karakterišu i izraženom varijabilnošću kariotipa. Do sada su opisane čak 74 različite hromozomske forme (HF), ali su zvanično priznate samo tri vrste.1 Svaka je zapravo kompleks vrsta sa statusom „nedovoljno podataka“ na IUCN Red List of Threatened Species. Evropske vrste ovog roda sačinjavaju kompleks N. leucodon supersp.1 Među njima je više od 20 različitih HF, od kojih je pet (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) prisutno u Srbiji, a poslednje dve se vode kao ugrožene.2 Iako su međusobno reproduktivno izolovane i filogenetski udaljene, zbog nedostatka morfoloških razlika predstavljaju kriptične vrste. Sa konzervacionog aspekta je veoma važno vršiti monitoring njihovih populacija i utvrditi areale rasprostanjenja. Stoga je neophodno razviti pouzdanu i neinvazivnu metodu za njihovu efikasnu determinaciju. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) tehnika, koja produkuje ISSR markere – anonimne sekvence DNK ograničene invertovanim tandemskim ponovcima, se pokazala kao korisna u identifikaciji morfološki sličnih vrsta kod nekoliko sisarskih taksona.3 Od 11 različitih ISSR prajmera testiranih na prisustvo fragmenata DNK specifičnih za vrstu, na ukupnom uzorku od 25 jedinki, tri prajmera (CAA)5GC; (CAG)4AC i (GA)8AC su dala informativne profile DNK, obezbeđujući pouzdano i jasno prepoznavanje svih pet analiziranih kriptičnih vrsta., Врсте слепог кучета из рода Nannospalax се, поред бројних адаптација на подземни начин живота, карактеришу и израженом варијабилношћу кариотипа. До сада су описане чак 74 различите хромозомске форме (ХФ), али су званично признате само три врсте.1 Свака је заправо комплекс врста са статусом „недовољно података“ на IUCN Red List of Threatened Species. Европске врсте овог рода сачињавају комплекс N. leucodon supersp.1 Међу њима је више од 20 различитих ХФ, од којих је пет (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) присутно у Србији, а последње две се воде као угрожене.2 Иако су међусобно репродуктивно изоловане и филогенетски удаљене, због недостатка морфолошких разлика представљају криптичне врсте. Са конзервационог аспекта је веома важно вршити мониторинг њихових популација и утврдити ареале распростањења. Стога је неопходно развити поуздану и неинвазивну методу за њихову ефикасну детерминацију. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) техника, која продукује ISSR маркере – анонимне секвенце ДНК ограничене инвертованим тандемским поновцима, се показала као корисна у идентификацији морфолошки сличних врста код неколико сисарских таксона.3 Од 11 различитих ISSR прајмера тестираних на присуство фрагмената ДНК специфичних за врсту, на укупном узорку од 25 јединки, три прајмера (CAA)5GC; (CAG)4AC и (GA)8AC су дала информативне профиле ДНК, обезбеђујући поуздано и јасно препознавање свих пет анализираних криптичних врста.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia), ISSR-PCR као једноставна дијагностичка метода за разликовање криптичних врста Nannospalax leucodon (Spalacinae, Rodentia)",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5312"
}

Đokić, M., Stamenković, G., Jojić, V., Ćosić, N., Ćirović, D., Savić, I.,& Bugarski-Stanojević, V.. (2022). ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 106.
https://hdl.handle.net/21.15107/rcub_ibiss_5312

Đokić M, Stamenković G, Jojić V, Ćosić N, Ćirović D, Savić I, Bugarski-Stanojević V. ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:106.
https://hdl.handle.net/21.15107/rcub_ibiss_5312 .

Đokić, Marko, Stamenković, Gorana, Jojić, Vida, Ćosić, Nada, Ćirović, Duško, Savić, Ivo, Bugarski-Stanojević, Vanja, "ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):106,
https://hdl.handle.net/21.15107/rcub_ibiss_5312 .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s42991-020-00019-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3660
AB  - The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.
PB  - Springer
T2  - Mammalian Biology
T1  - 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)
VL  - 100
DO  - 10.1007/s42991-020-00019-9
SP  - 315
EP  - 324
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2020",
abstract = "The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.",
publisher = "Springer",
journal = "Mammalian Biology",
title = "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)",
volume = "100",
doi = "10.1007/s42991-020-00019-9",
pages = "315-324"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2020). 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology
Springer., 100, 315-324.
https://doi.org/10.1007/s42991-020-00019-9

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology. 2020;100:315-324.
doi:10.1007/s42991-020-00019-9 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)" in Mammalian Biology, 100 (2020):315-324,
https://doi.org/10.1007/s42991-020-00019-9 . .

TY  - CONF
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5657
AB  - Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5657
ER  - 

@conference{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2019",
abstract = "Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5657"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2019). 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5657

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

TY  - JOUR
AU  - Savić, Ivo
AU  - Ćirović, Duško
AU  - Bugarski-Stanojević, Vanja
PY  - 2017
UR  - http://www.mdpi.com/2073-4425/8/11/292
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2895
AB  - Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.
T2  - Genes
T1  - Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe
IS  - 11
VL  - 8
DO  - 10.3390/genes8110292
SP  - 292
EP  - 292
ER  - 

@article{
author = "Savić, Ivo and Ćirović, Duško and Bugarski-Stanojević, Vanja",
year = "2017",
abstract = "Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.",
journal = "Genes",
title = "Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe",
number = "11",
volume = "8",
doi = "10.3390/genes8110292",
pages = "292-292"
}

Savić, I., Ćirović, D.,& Bugarski-Stanojević, V.. (2017). Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe. in Genes, 8(11), 292-292.
https://doi.org/10.3390/genes8110292

Savić I, Ćirović D, Bugarski-Stanojević V. Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe. in Genes. 2017;8(11):292-292.
doi:10.3390/genes8110292 .

Savić, Ivo, Ćirović, Duško, Bugarski-Stanojević, Vanja, "Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe" in Genes, 8, no. 11 (2017):292-292,
https://doi.org/10.3390/genes8110292 . .