TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6592
AB  - Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6592
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6592"
}

Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kolarević, S.. (2023). In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592

Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kolarević S. In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kolarević, Stoimir, "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac024/6823671
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5243
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac024
SP  - 21
EP  - 32
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024",
pages = "21-32"
}

Jovanović Marić, J., Kolarević, S., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford: Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
AU  - Žegura, Bojana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6233
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays
SP  - 160
EP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6233
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Oswald, Peter and Slobodnik, Jaroslav and Paunović, Momir and Vuković-Gačić, Branka and Žegura, Bojana",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays",
pages = "160-161",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6233"
}

Kolarević, S., Kračun-Kolarević, M., Oswald, P., Slobodnik, J., Paunović, M., Vuković-Gačić, B.,& Žegura, B.. (2022). Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 160-161.
https://hdl.handle.net/21.15107/rcub_ibiss_6233

Kolarević S, Kračun-Kolarević M, Oswald P, Slobodnik J, Paunović M, Vuković-Gačić B, Žegura B. Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:160-161.
https://hdl.handle.net/21.15107/rcub_ibiss_6233 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Oswald, Peter, Slobodnik, Jaroslav, Paunović, Momir, Vuković-Gačić, Branka, Žegura, Bojana, "Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):160-161,
https://hdl.handle.net/21.15107/rcub_ibiss_6233 .

TY  - JOUR
AU  - Kittner, Maria
AU  - Kerndorff, Alexander
AU  - Ricking, Mathias
AU  - Bednarz, Marius
AU  - Obermaier, Nathan
AU  - Lukas, Marcus
AU  - Asenova, Mina
AU  - Bordós, Gábor
AU  - Eisentraut, Paul
AU  - Hohenblum, Philipp
AU  - Hudcova, Hana
AU  - Humer, Franko
AU  - István, Tóth György
AU  - Kirchner, Michal
AU  - Marushevska, Olena
AU  - Nemejcová, Denisa
AU  - Oswald, Peter
AU  - Paunović, Momir
AU  - Sengl, Manfred
AU  - Slobodnik, Jaroslav
AU  - Spanowsky, Karl
AU  - Tudorache, Madalina
AU  - Wagensonner, Helmut
AU  - Liska, Igor
AU  - Braun, Ulrike
AU  - Bannick, Claus G.
PY  - 2022
UR  - https://pubs.acs.org/doi/10.1021/acsestwater.1c00439
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5074
AB  - In this study, carried out within the Joint Danube Survey 4, a comprehensive microplastic screening in the water column within a large European river basin from its source to estuary, including major tributaries, was realized. The objective was to develop principles of a systematic and practicable microplastic monitoring strategy using sedimentation boxes for collection of suspended particulate matter followed by its subsequent analysis using thermal extraction desorption-gas chromatography/mass spectrometry. In total, 18 sampling sites in the Danube River Basin were investigated. The obtained suspended particulate matter samples were subdivided into the fractions of >100 mu m and <100 mu m and subsequently analyzed for microplastic mass contents. The results showed that microplastics were detected in all samples, with polyethylene being the predominant polymer with maximum contents of 22.24 mu g/mg, 3.23 mu g/mg for polystyrene, 1.03 mu g/mg for styrene-butadiene-rubber, and 0.45 mu g/mg for polypropylene. Further, polymers such as different sorts of polyester, polyacrylates, polylactide, and natural rubber were not detected or below the detection limit. Additional investigations on possible interference of polyethylene signals by algae-derived fatty acids were assessed. In the context of targeted monitoring, repeated measurements provide more certainty in the interpretation of the results for the individual sites. Nevertheless, it can be stated that the chosen approach using an integrative sampling and determination of total plastic content proved to be successful.
PB  - Washington: American Chemical Society
T2  - ACS ES&T Water
T1  - Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach
IS  - 7
VL  - 2
DO  - 10.1021/acsestwater.1c00439
SP  - 1174
EP  - 1181
ER  - 

@article{
author = "Kittner, Maria and Kerndorff, Alexander and Ricking, Mathias and Bednarz, Marius and Obermaier, Nathan and Lukas, Marcus and Asenova, Mina and Bordós, Gábor and Eisentraut, Paul and Hohenblum, Philipp and Hudcova, Hana and Humer, Franko and István, Tóth György and Kirchner, Michal and Marushevska, Olena and Nemejcová, Denisa and Oswald, Peter and Paunović, Momir and Sengl, Manfred and Slobodnik, Jaroslav and Spanowsky, Karl and Tudorache, Madalina and Wagensonner, Helmut and Liska, Igor and Braun, Ulrike and Bannick, Claus G.",
year = "2022",
abstract = "In this study, carried out within the Joint Danube Survey 4, a comprehensive microplastic screening in the water column within a large European river basin from its source to estuary, including major tributaries, was realized. The objective was to develop principles of a systematic and practicable microplastic monitoring strategy using sedimentation boxes for collection of suspended particulate matter followed by its subsequent analysis using thermal extraction desorption-gas chromatography/mass spectrometry. In total, 18 sampling sites in the Danube River Basin were investigated. The obtained suspended particulate matter samples were subdivided into the fractions of >100 mu m and <100 mu m and subsequently analyzed for microplastic mass contents. The results showed that microplastics were detected in all samples, with polyethylene being the predominant polymer with maximum contents of 22.24 mu g/mg, 3.23 mu g/mg for polystyrene, 1.03 mu g/mg for styrene-butadiene-rubber, and 0.45 mu g/mg for polypropylene. Further, polymers such as different sorts of polyester, polyacrylates, polylactide, and natural rubber were not detected or below the detection limit. Additional investigations on possible interference of polyethylene signals by algae-derived fatty acids were assessed. In the context of targeted monitoring, repeated measurements provide more certainty in the interpretation of the results for the individual sites. Nevertheless, it can be stated that the chosen approach using an integrative sampling and determination of total plastic content proved to be successful.",
publisher = "Washington: American Chemical Society",
journal = "ACS ES&T Water",
title = "Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach",
number = "7",
volume = "2",
doi = "10.1021/acsestwater.1c00439",
pages = "1174-1181"
}

Kittner, M., Kerndorff, A., Ricking, M., Bednarz, M., Obermaier, N., Lukas, M., Asenova, M., Bordós, G., Eisentraut, P., Hohenblum, P., Hudcova, H., Humer, F., István, T. G., Kirchner, M., Marushevska, O., Nemejcová, D., Oswald, P., Paunović, M., Sengl, M., Slobodnik, J., Spanowsky, K., Tudorache, M., Wagensonner, H., Liska, I., Braun, U.,& Bannick, C. G.. (2022). Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach. in ACS ES&T Water
Washington: American Chemical Society., 2(7), 1174-1181.
https://doi.org/10.1021/acsestwater.1c00439

Kittner M, Kerndorff A, Ricking M, Bednarz M, Obermaier N, Lukas M, Asenova M, Bordós G, Eisentraut P, Hohenblum P, Hudcova H, Humer F, István TG, Kirchner M, Marushevska O, Nemejcová D, Oswald P, Paunović M, Sengl M, Slobodnik J, Spanowsky K, Tudorache M, Wagensonner H, Liska I, Braun U, Bannick CG. Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach. in ACS ES&T Water. 2022;2(7):1174-1181.
doi:10.1021/acsestwater.1c00439 .

Kittner, Maria, Kerndorff, Alexander, Ricking, Mathias, Bednarz, Marius, Obermaier, Nathan, Lukas, Marcus, Asenova, Mina, Bordós, Gábor, Eisentraut, Paul, Hohenblum, Philipp, Hudcova, Hana, Humer, Franko, István, Tóth György, Kirchner, Michal, Marushevska, Olena, Nemejcová, Denisa, Oswald, Peter, Paunović, Momir, Sengl, Manfred, Slobodnik, Jaroslav, Spanowsky, Karl, Tudorache, Madalina, Wagensonner, Helmut, Liska, Igor, Braun, Ulrike, Bannick, Claus G., "Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach" in ACS ES&T Water, 2, no. 7 (2022):1174-1181,
https://doi.org/10.1021/acsestwater.1c00439 . .