TY  - JOUR
AU  - Sudar, Emina M
AU  - Dobutović, Branislava D
AU  - Soskić, Sanja S
AU  - Mandušić, Vesna
AU  - Zakula, Zorica
AU  - Misirkić Marjanović, Maja
AU  - Vučićević, Ljubica
AU  - Janjetović, Kristina
AU  - Trajković, Vladimir S
AU  - Mikhailidis, Dimitri P
AU  - Isenović, Esma R
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1121
AB  - The purpose of this study was to examine the effects of ghrelin on protein kinase B (Akt) and mitogen-activated protein kinase p42/44 (ERK1/2) activation as well as ghrelin effects on inducible nitric oxide (NO) synthase (iNOS; for gene Nos2) activity/expression in rat hearts. Male Wistar rats were treated with ghrelin (0.3 nmol/5 mu l) or an equal volume of phosphate-buffered saline, injected every 24 h into the lateral cerebral ventricle for 5 days and 2 h after the last treatment the animals were sacrificed. Serum NO, l-arginine (l-Arg), and arginase activity were measured spectrophotometrically. For phosphorylation of Akt, ERK1/2, and iNOS protein expression, Western blot method was used. The expression of Nos2 mRNA was measured by the quantitative real-time polymerase chain reaction (qRT-PCR). Treatment with ghrelin significantly increased NO production in serum by 1.4-fold compared with control. The concentration of l-Arg was significantly higher in ghrelin-treated rats than in control while arginase activity was significantly lower in ghrelin-treated than in control hearts. Ghrelin treatment increased phosphorylation of Akt by 1.9-fold and ERK1/2 by 1.6-fold and increased iNOS expression by 2.5-fold compared with control. In addition, ghrelin treatment increased Nos2 gene expression by 2.2-fold as determined by qRT-PCR. These results indicate that ghrelin regulation of iNOS expression/activity is mediated via Akt/ERK1/2 signaling pathway. These results may be relevant to understanding molecular mechanisms underlying direct cardiovascular actions of ghrelin.
T2  - Journal of Physiology and Biochemistry
T1  - Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats
IS  - 2
VL  - 67
SP  - 483
EP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1121
ER  - 

@article{
author = "Sudar, Emina M and Dobutović, Branislava D and Soskić, Sanja S and Mandušić, Vesna and Zakula, Zorica and Misirkić Marjanović, Maja and Vučićević, Ljubica and Janjetović, Kristina and Trajković, Vladimir S and Mikhailidis, Dimitri P and Isenović, Esma R",
year = "2011",
abstract = "The purpose of this study was to examine the effects of ghrelin on protein kinase B (Akt) and mitogen-activated protein kinase p42/44 (ERK1/2) activation as well as ghrelin effects on inducible nitric oxide (NO) synthase (iNOS; for gene Nos2) activity/expression in rat hearts. Male Wistar rats were treated with ghrelin (0.3 nmol/5 mu l) or an equal volume of phosphate-buffered saline, injected every 24 h into the lateral cerebral ventricle for 5 days and 2 h after the last treatment the animals were sacrificed. Serum NO, l-arginine (l-Arg), and arginase activity were measured spectrophotometrically. For phosphorylation of Akt, ERK1/2, and iNOS protein expression, Western blot method was used. The expression of Nos2 mRNA was measured by the quantitative real-time polymerase chain reaction (qRT-PCR). Treatment with ghrelin significantly increased NO production in serum by 1.4-fold compared with control. The concentration of l-Arg was significantly higher in ghrelin-treated rats than in control while arginase activity was significantly lower in ghrelin-treated than in control hearts. Ghrelin treatment increased phosphorylation of Akt by 1.9-fold and ERK1/2 by 1.6-fold and increased iNOS expression by 2.5-fold compared with control. In addition, ghrelin treatment increased Nos2 gene expression by 2.2-fold as determined by qRT-PCR. These results indicate that ghrelin regulation of iNOS expression/activity is mediated via Akt/ERK1/2 signaling pathway. These results may be relevant to understanding molecular mechanisms underlying direct cardiovascular actions of ghrelin.",
journal = "Journal of Physiology and Biochemistry",
title = "Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats",
number = "2",
volume = "67",
pages = "483-204",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1121"
}

Sudar, E. M., Dobutović, B. D., Soskić, S. S., Mandušić, V., Zakula, Z., Misirkić Marjanović, M., Vučićević, L., Janjetović, K., Trajković, V. S., Mikhailidis, D. P.,& Isenović, E. R.. (2011). Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats. in Journal of Physiology and Biochemistry, 67(2), 483-204.
https://hdl.handle.net/21.15107/rcub_ibiss_1121

Sudar EM, Dobutović BD, Soskić SS, Mandušić V, Zakula Z, Misirkić Marjanović M, Vučićević L, Janjetović K, Trajković VS, Mikhailidis DP, Isenović ER. Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats. in Journal of Physiology and Biochemistry. 2011;67(2):483-204.
https://hdl.handle.net/21.15107/rcub_ibiss_1121 .

Sudar, Emina M, Dobutović, Branislava D, Soskić, Sanja S, Mandušić, Vesna, Zakula, Zorica, Misirkić Marjanović, Maja, Vučićević, Ljubica, Janjetović, Kristina, Trajković, Vladimir S, Mikhailidis, Dimitri P, Isenović, Esma R, "Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats" in Journal of Physiology and Biochemistry, 67, no. 2 (2011):483-204,
https://hdl.handle.net/21.15107/rcub_ibiss_1121 .