TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6592
AB  - Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6592
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6592"
}

Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kolarević, S.. (2023). In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592

Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kolarević S. In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kolarević, Stoimir, "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac024/6823671
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5243
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac024
SP  - 21
EP  - 32
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024",
pages = "21-32"
}

Jovanović Marić, J., Kolarević, S., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford: Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Oswald, Petar
AU  - Oswaldova, Martina
AU  - Slobodnik, Jaroslav
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kološa, Katja
AU  - Žegura, Bojana
AU  - Kolarević, Stoimir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5725
AB  - Within the Joint Danube Survey 4 (JDS4) (2019) surface water samples
were collected at 24 localities along the Danube River and were extracted by
horizon large volume solid-phase extraction (LVSPE) technique. LVSPE
technique provided 25,000x relative enrichment factor (REF) of the river
samples. The bioactivity of the LVSPE samples was investigated employing
prokaryotic SOS/umuC assay (S9+ and S9-) and battery of bioassays
analyzing different endpoints – cytotoxicity (MTS assay), genotoxicity
(comet assay), cell cycle analyses of ZFL cells and embryo toxicity (zFET) in
eukaryotic models (zebrafish liver cell line – ZFL and Danio rerio embryos –
zebrafish). In the case of the SOS/umuC assay none of the tested samples
(REF100) has induced genotoxic effect. The cytotoxic effect was detected for
11 samples at REF100 (MTS assay). The comet assay results indicated
genotoxic potential for nine samples – one in the Upper Danube, six in the
Middle Danube and two in the Lower Danube. The most potent were
JDS4-37 and JDS4-41 samples collected at two sites in the Serbian part of
the Danube River. These samples were analyzed at REF13. The analysis of
the cell cycle was performed in the case of eight samples for which were
previously recorded genotoxic effect. For one sample (JDS4-41) was found
the G1 phase cell cycle arrest. The zFET assay was performed for all the
samples at REF100 and there was no embryo toxicity or teratogenicity
recorded. The results obtained within this study indicate a higher sensitivity
of eukaryotic models compared to prokaryotic SOS/umuC assay. Likewise, it
was found that most of the genotoxic samples originate from the middle
part of the Danube.
PB  - European Environmental Mutagenesis & Genomics Society
C3  - Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
T1  - Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5725
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Oswald, Petar and Oswaldova, Martina and Slobodnik, Jaroslav and Vuković-Gačić, Branka and Paunović, Momir and Kološa, Katja and Žegura, Bojana and Kolarević, Stoimir",
year = "2022",
abstract = "Within the Joint Danube Survey 4 (JDS4) (2019) surface water samples
were collected at 24 localities along the Danube River and were extracted by
horizon large volume solid-phase extraction (LVSPE) technique. LVSPE
technique provided 25,000x relative enrichment factor (REF) of the river
samples. The bioactivity of the LVSPE samples was investigated employing
prokaryotic SOS/umuC assay (S9+ and S9-) and battery of bioassays
analyzing different endpoints – cytotoxicity (MTS assay), genotoxicity
(comet assay), cell cycle analyses of ZFL cells and embryo toxicity (zFET) in
eukaryotic models (zebrafish liver cell line – ZFL and Danio rerio embryos –
zebrafish). In the case of the SOS/umuC assay none of the tested samples
(REF100) has induced genotoxic effect. The cytotoxic effect was detected for
11 samples at REF100 (MTS assay). The comet assay results indicated
genotoxic potential for nine samples – one in the Upper Danube, six in the
Middle Danube and two in the Lower Danube. The most potent were
JDS4-37 and JDS4-41 samples collected at two sites in the Serbian part of
the Danube River. These samples were analyzed at REF13. The analysis of
the cell cycle was performed in the case of eight samples for which were
previously recorded genotoxic effect. For one sample (JDS4-41) was found
the G1 phase cell cycle arrest. The zFET assay was performed for all the
samples at REF100 and there was no embryo toxicity or teratogenicity
recorded. The results obtained within this study indicate a higher sensitivity
of eukaryotic models compared to prokaryotic SOS/umuC assay. Likewise, it
was found that most of the genotoxic samples originate from the middle
part of the Danube.",
publisher = "European Environmental Mutagenesis & Genomics Society",
journal = "Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands",
title = "Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5725"
}

Kračun-Kolarević, M., Jovanović Marić, J., Oswald, P., Oswaldova, M., Slobodnik, J., Vuković-Gačić, B., Paunović, M., Kološa, K., Žegura, B.,& Kolarević, S.. (2022). Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands
European Environmental Mutagenesis & Genomics Society., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5725

Kračun-Kolarević M, Jovanović Marić J, Oswald P, Oswaldova M, Slobodnik J, Vuković-Gačić B, Paunović M, Kološa K, Žegura B, Kolarević S. Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique.. in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands. 2022;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5725 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Oswald, Petar, Oswaldova, Martina, Slobodnik, Jaroslav, Vuković-Gačić, Branka, Paunović, Momir, Kološa, Katja, Žegura, Bojana, Kolarević, Stoimir, "Bioactivity of the Joint Danube Survey 4 surface water samples collected by horizon large volume solid–phase extraction technique." in Joint EEMGS meeting & International Comet Assay Workshop; 2022 May 23-26; Maastricht, The Netherlands (2022):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5725 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
AU  - Žegura, Bojana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6233
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays
SP  - 160
EP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6233
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Oswald, Peter and Slobodnik, Jaroslav and Paunović, Momir and Vuković-Gačić, Branka and Žegura, Bojana",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays",
pages = "160-161",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6233"
}

Kolarević, S., Kračun-Kolarević, M., Oswald, P., Slobodnik, J., Paunović, M., Vuković-Gačić, B.,& Žegura, B.. (2022). Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 160-161.
https://hdl.handle.net/21.15107/rcub_ibiss_6233

Kolarević S, Kračun-Kolarević M, Oswald P, Slobodnik J, Paunović M, Vuković-Gačić B, Žegura B. Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:160-161.
https://hdl.handle.net/21.15107/rcub_ibiss_6233 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Oswald, Peter, Slobodnik, Jaroslav, Paunović, Momir, Vuković-Gačić, Branka, Žegura, Bojana, "Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):160-161,
https://hdl.handle.net/21.15107/rcub_ibiss_6233 .

TY  - JOUR
AU  - Kittner, Maria
AU  - Kerndorff, Alexander
AU  - Ricking, Mathias
AU  - Bednarz, Marius
AU  - Obermaier, Nathan
AU  - Lukas, Marcus
AU  - Asenova, Mina
AU  - Bordós, Gábor
AU  - Eisentraut, Paul
AU  - Hohenblum, Philipp
AU  - Hudcova, Hana
AU  - Humer, Franko
AU  - István, Tóth György
AU  - Kirchner, Michal
AU  - Marushevska, Olena
AU  - Nemejcová, Denisa
AU  - Oswald, Peter
AU  - Paunović, Momir
AU  - Sengl, Manfred
AU  - Slobodnik, Jaroslav
AU  - Spanowsky, Karl
AU  - Tudorache, Madalina
AU  - Wagensonner, Helmut
AU  - Liska, Igor
AU  - Braun, Ulrike
AU  - Bannick, Claus G.
PY  - 2022
UR  - https://pubs.acs.org/doi/10.1021/acsestwater.1c00439
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5074
AB  - In this study, carried out within the Joint Danube Survey 4, a comprehensive microplastic screening in the water column within a large European river basin from its source to estuary, including major tributaries, was realized. The objective was to develop principles of a systematic and practicable microplastic monitoring strategy using sedimentation boxes for collection of suspended particulate matter followed by its subsequent analysis using thermal extraction desorption-gas chromatography/mass spectrometry. In total, 18 sampling sites in the Danube River Basin were investigated. The obtained suspended particulate matter samples were subdivided into the fractions of >100 mu m and <100 mu m and subsequently analyzed for microplastic mass contents. The results showed that microplastics were detected in all samples, with polyethylene being the predominant polymer with maximum contents of 22.24 mu g/mg, 3.23 mu g/mg for polystyrene, 1.03 mu g/mg for styrene-butadiene-rubber, and 0.45 mu g/mg for polypropylene. Further, polymers such as different sorts of polyester, polyacrylates, polylactide, and natural rubber were not detected or below the detection limit. Additional investigations on possible interference of polyethylene signals by algae-derived fatty acids were assessed. In the context of targeted monitoring, repeated measurements provide more certainty in the interpretation of the results for the individual sites. Nevertheless, it can be stated that the chosen approach using an integrative sampling and determination of total plastic content proved to be successful.
PB  - Washington: American Chemical Society
T2  - ACS ES&T Water
T1  - Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach
IS  - 7
VL  - 2
DO  - 10.1021/acsestwater.1c00439
SP  - 1174
EP  - 1181
ER  - 

@article{
author = "Kittner, Maria and Kerndorff, Alexander and Ricking, Mathias and Bednarz, Marius and Obermaier, Nathan and Lukas, Marcus and Asenova, Mina and Bordós, Gábor and Eisentraut, Paul and Hohenblum, Philipp and Hudcova, Hana and Humer, Franko and István, Tóth György and Kirchner, Michal and Marushevska, Olena and Nemejcová, Denisa and Oswald, Peter and Paunović, Momir and Sengl, Manfred and Slobodnik, Jaroslav and Spanowsky, Karl and Tudorache, Madalina and Wagensonner, Helmut and Liska, Igor and Braun, Ulrike and Bannick, Claus G.",
year = "2022",
abstract = "In this study, carried out within the Joint Danube Survey 4, a comprehensive microplastic screening in the water column within a large European river basin from its source to estuary, including major tributaries, was realized. The objective was to develop principles of a systematic and practicable microplastic monitoring strategy using sedimentation boxes for collection of suspended particulate matter followed by its subsequent analysis using thermal extraction desorption-gas chromatography/mass spectrometry. In total, 18 sampling sites in the Danube River Basin were investigated. The obtained suspended particulate matter samples were subdivided into the fractions of >100 mu m and <100 mu m and subsequently analyzed for microplastic mass contents. The results showed that microplastics were detected in all samples, with polyethylene being the predominant polymer with maximum contents of 22.24 mu g/mg, 3.23 mu g/mg for polystyrene, 1.03 mu g/mg for styrene-butadiene-rubber, and 0.45 mu g/mg for polypropylene. Further, polymers such as different sorts of polyester, polyacrylates, polylactide, and natural rubber were not detected or below the detection limit. Additional investigations on possible interference of polyethylene signals by algae-derived fatty acids were assessed. In the context of targeted monitoring, repeated measurements provide more certainty in the interpretation of the results for the individual sites. Nevertheless, it can be stated that the chosen approach using an integrative sampling and determination of total plastic content proved to be successful.",
publisher = "Washington: American Chemical Society",
journal = "ACS ES&T Water",
title = "Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach",
number = "7",
volume = "2",
doi = "10.1021/acsestwater.1c00439",
pages = "1174-1181"
}

Kittner, M., Kerndorff, A., Ricking, M., Bednarz, M., Obermaier, N., Lukas, M., Asenova, M., Bordós, G., Eisentraut, P., Hohenblum, P., Hudcova, H., Humer, F., István, T. G., Kirchner, M., Marushevska, O., Nemejcová, D., Oswald, P., Paunović, M., Sengl, M., Slobodnik, J., Spanowsky, K., Tudorache, M., Wagensonner, H., Liska, I., Braun, U.,& Bannick, C. G.. (2022). Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach. in ACS ES&T Water
Washington: American Chemical Society., 2(7), 1174-1181.
https://doi.org/10.1021/acsestwater.1c00439

Kittner M, Kerndorff A, Ricking M, Bednarz M, Obermaier N, Lukas M, Asenova M, Bordós G, Eisentraut P, Hohenblum P, Hudcova H, Humer F, István TG, Kirchner M, Marushevska O, Nemejcová D, Oswald P, Paunović M, Sengl M, Slobodnik J, Spanowsky K, Tudorache M, Wagensonner H, Liska I, Braun U, Bannick CG. Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach. in ACS ES&T Water. 2022;2(7):1174-1181.
doi:10.1021/acsestwater.1c00439 .

Kittner, Maria, Kerndorff, Alexander, Ricking, Mathias, Bednarz, Marius, Obermaier, Nathan, Lukas, Marcus, Asenova, Mina, Bordós, Gábor, Eisentraut, Paul, Hohenblum, Philipp, Hudcova, Hana, Humer, Franko, István, Tóth György, Kirchner, Michal, Marushevska, Olena, Nemejcová, Denisa, Oswald, Peter, Paunović, Momir, Sengl, Manfred, Slobodnik, Jaroslav, Spanowsky, Karl, Tudorache, Madalina, Wagensonner, Helmut, Liska, Igor, Braun, Ulrike, Bannick, Claus G., "Microplastics in the Danube River Basin: A First Comprehensive Screening with a Harmonized Analytical Approach" in ACS ES&T Water, 2, no. 7 (2022):1174-1181,
https://doi.org/10.1021/acsestwater.1c00439 . .

TY  - JOUR
AU  - Lundy, Lian
AU  - Fatta-Kassinos, Despo
AU  - Slobodnik, Jaroslav
AU  - Karaolia, Popi
AU  - Cirka, Lubos
AU  - Kreuzinger, Norbert
AU  - Castiglioni, Sara
AU  - Bijlsma, Lubertus
AU  - Dulio, Valeria
AU  - Deviller, Geneviève
AU  - Lai, Foon Yin
AU  - Alygizakis, Nikiforos
AU  - Barneo, Manuela
AU  - Baz-Lomba, Jose Antonio
AU  - Béen, Frederic
AU  - Cíchová, Marianna
AU  - Conde-Pérez, Kelly
AU  - Covaci, Adrian
AU  - Donner, Erica
AU  - Ficek, Andrej
AU  - Hassard, Francis
AU  - Hedström, Annelie
AU  - Hernandez, Félix
AU  - Janská, Veronika
AU  - Jellison, Kristen
AU  - Hofman, Jan
AU  - Hill, Kelly
AU  - Hong, Pei-Ying
AU  - Kasprzyk-Hordern, Barbara
AU  - Kolarević, Stoimir
AU  - Krahulec, Jan
AU  - Lambropoulou, Dimitra
AU  - de Llanos, Rosa
AU  - Mackuľak, Tomáš
AU  - Martinez-García, Lorena
AU  - Martínez, Francisco
AU  - Medema, Gertjan
AU  - Micsinai, Adrienn
AU  - Myrmel, Mette
AU  - Nasser, Mohammed
AU  - Niederstätter, Harald
AU  - Nozal, Leonor
AU  - Oberacher, Herbert
AU  - Očenášková, Věra
AU  - Ogorzaly, Leslie
AU  - Papadopoulos, Dimitrios
AU  - Peinado, Beatriz
AU  - Pitkänen, Tarja
AU  - Poza, Margarita
AU  - Rumbo-Feal, Soraya
AU  - Sánchez, Maria Blanca
AU  - Székely, Anna J.
AU  - Soltysova, Andrea
AU  - Thomaidis, Nikolaos S.
AU  - Vallejo, Juan
AU  - van Nuijs, Alexander
AU  - Ware, Vassie
AU  - Viklander, Maria
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4246
AB  - The presence of SARS-CoV-2 RNA in wastewater was first reported in March 2020. Over the subsequent months, the potential for wastewater surveillance to contribute to COVID-19 mitigation programmes has been the focus of intense national and international research activities, gaining the attention of policy makers and the public. As a new application of an established methodology, focused collaboration between public health practitioners and wastewater researchers is essential to developing a common understanding on how, when and where the outputs of this non-invasive community-level approach can deliver actionable outcomes for public health authorities. Within this context, the NORMAN SCORE “SARS-CoV-2 in sewage” database provides a platform for rapid, open access data sharing, validated by the uploading of 276 data sets from nine countries to-date. Through offering direct access to underpinning meta-data sets (and describing its use in data interpretation), the NORMAN SCORE database is a resource for the development of recommendations on minimum data requirements for wastewater pathogen surveillance. It is also a tool to engage public health practitioners in discussions on use of the approach, providing an opportunity to build mutual understanding of the demand and supply for data and facilitate the translation of this promising research application into public health practice.
PB  - Elsevier BV
T2  - Water Research
T1  - Making Waves: Collaboration in the time of SARS-CoV-2 - rapid development of an international co-operation and wastewater surveillance database to support public health decision-making
VL  - 199
DO  - 10.1016/j.watres.2021.117167
SP  - 117167
ER  - 

@article{
author = "Lundy, Lian and Fatta-Kassinos, Despo and Slobodnik, Jaroslav and Karaolia, Popi and Cirka, Lubos and Kreuzinger, Norbert and Castiglioni, Sara and Bijlsma, Lubertus and Dulio, Valeria and Deviller, Geneviève and Lai, Foon Yin and Alygizakis, Nikiforos and Barneo, Manuela and Baz-Lomba, Jose Antonio and Béen, Frederic and Cíchová, Marianna and Conde-Pérez, Kelly and Covaci, Adrian and Donner, Erica and Ficek, Andrej and Hassard, Francis and Hedström, Annelie and Hernandez, Félix and Janská, Veronika and Jellison, Kristen and Hofman, Jan and Hill, Kelly and Hong, Pei-Ying and Kasprzyk-Hordern, Barbara and Kolarević, Stoimir and Krahulec, Jan and Lambropoulou, Dimitra and de Llanos, Rosa and Mackuľak, Tomáš and Martinez-García, Lorena and Martínez, Francisco and Medema, Gertjan and Micsinai, Adrienn and Myrmel, Mette and Nasser, Mohammed and Niederstätter, Harald and Nozal, Leonor and Oberacher, Herbert and Očenášková, Věra and Ogorzaly, Leslie and Papadopoulos, Dimitrios and Peinado, Beatriz and Pitkänen, Tarja and Poza, Margarita and Rumbo-Feal, Soraya and Sánchez, Maria Blanca and Székely, Anna J. and Soltysova, Andrea and Thomaidis, Nikolaos S. and Vallejo, Juan and van Nuijs, Alexander and Ware, Vassie and Viklander, Maria",
year = "2021",
abstract = "The presence of SARS-CoV-2 RNA in wastewater was first reported in March 2020. Over the subsequent months, the potential for wastewater surveillance to contribute to COVID-19 mitigation programmes has been the focus of intense national and international research activities, gaining the attention of policy makers and the public. As a new application of an established methodology, focused collaboration between public health practitioners and wastewater researchers is essential to developing a common understanding on how, when and where the outputs of this non-invasive community-level approach can deliver actionable outcomes for public health authorities. Within this context, the NORMAN SCORE “SARS-CoV-2 in sewage” database provides a platform for rapid, open access data sharing, validated by the uploading of 276 data sets from nine countries to-date. Through offering direct access to underpinning meta-data sets (and describing its use in data interpretation), the NORMAN SCORE database is a resource for the development of recommendations on minimum data requirements for wastewater pathogen surveillance. It is also a tool to engage public health practitioners in discussions on use of the approach, providing an opportunity to build mutual understanding of the demand and supply for data and facilitate the translation of this promising research application into public health practice.",
publisher = "Elsevier BV",
journal = "Water Research",
title = "Making Waves: Collaboration in the time of SARS-CoV-2 - rapid development of an international co-operation and wastewater surveillance database to support public health decision-making",
volume = "199",
doi = "10.1016/j.watres.2021.117167",
pages = "117167"
}

Lundy, L., Fatta-Kassinos, D., Slobodnik, J., Karaolia, P., Cirka, L., Kreuzinger, N., Castiglioni, S., Bijlsma, L., Dulio, V., Deviller, G., Lai, F. Y., Alygizakis, N., Barneo, M., Baz-Lomba, J. A., Béen, F., Cíchová, M., Conde-Pérez, K., Covaci, A., Donner, E., Ficek, A., Hassard, F., Hedström, A., Hernandez, F., Janská, V., Jellison, K., Hofman, J., Hill, K., Hong, P., Kasprzyk-Hordern, B., Kolarević, S., Krahulec, J., Lambropoulou, D., de Llanos, R., Mackuľak, T., Martinez-García, L., Martínez, F., Medema, G., Micsinai, A., Myrmel, M., Nasser, M., Niederstätter, H., Nozal, L., Oberacher, H., Očenášková, V., Ogorzaly, L., Papadopoulos, D., Peinado, B., Pitkänen, T., Poza, M., Rumbo-Feal, S., Sánchez, M. B., Székely, A. J., Soltysova, A., Thomaidis, N. S., Vallejo, J., van Nuijs, A., Ware, V.,& Viklander, M.. (2021). Making Waves: Collaboration in the time of SARS-CoV-2 - rapid development of an international co-operation and wastewater surveillance database to support public health decision-making. in Water Research
Elsevier BV., 199, 117167.
https://doi.org/10.1016/j.watres.2021.117167

Lundy L, Fatta-Kassinos D, Slobodnik J, Karaolia P, Cirka L, Kreuzinger N, Castiglioni S, Bijlsma L, Dulio V, Deviller G, Lai FY, Alygizakis N, Barneo M, Baz-Lomba JA, Béen F, Cíchová M, Conde-Pérez K, Covaci A, Donner E, Ficek A, Hassard F, Hedström A, Hernandez F, Janská V, Jellison K, Hofman J, Hill K, Hong P, Kasprzyk-Hordern B, Kolarević S, Krahulec J, Lambropoulou D, de Llanos R, Mackuľak T, Martinez-García L, Martínez F, Medema G, Micsinai A, Myrmel M, Nasser M, Niederstätter H, Nozal L, Oberacher H, Očenášková V, Ogorzaly L, Papadopoulos D, Peinado B, Pitkänen T, Poza M, Rumbo-Feal S, Sánchez MB, Székely AJ, Soltysova A, Thomaidis NS, Vallejo J, van Nuijs A, Ware V, Viklander M. Making Waves: Collaboration in the time of SARS-CoV-2 - rapid development of an international co-operation and wastewater surveillance database to support public health decision-making. in Water Research. 2021;199:117167.
doi:10.1016/j.watres.2021.117167 .

Lundy, Lian, Fatta-Kassinos, Despo, Slobodnik, Jaroslav, Karaolia, Popi, Cirka, Lubos, Kreuzinger, Norbert, Castiglioni, Sara, Bijlsma, Lubertus, Dulio, Valeria, Deviller, Geneviève, Lai, Foon Yin, Alygizakis, Nikiforos, Barneo, Manuela, Baz-Lomba, Jose Antonio, Béen, Frederic, Cíchová, Marianna, Conde-Pérez, Kelly, Covaci, Adrian, Donner, Erica, Ficek, Andrej, Hassard, Francis, Hedström, Annelie, Hernandez, Félix, Janská, Veronika, Jellison, Kristen, Hofman, Jan, Hill, Kelly, Hong, Pei-Ying, Kasprzyk-Hordern, Barbara, Kolarević, Stoimir, Krahulec, Jan, Lambropoulou, Dimitra, de Llanos, Rosa, Mackuľak, Tomáš, Martinez-García, Lorena, Martínez, Francisco, Medema, Gertjan, Micsinai, Adrienn, Myrmel, Mette, Nasser, Mohammed, Niederstätter, Harald, Nozal, Leonor, Oberacher, Herbert, Očenášková, Věra, Ogorzaly, Leslie, Papadopoulos, Dimitrios, Peinado, Beatriz, Pitkänen, Tarja, Poza, Margarita, Rumbo-Feal, Soraya, Sánchez, Maria Blanca, Székely, Anna J., Soltysova, Andrea, Thomaidis, Nikolaos S., Vallejo, Juan, van Nuijs, Alexander, Ware, Vassie, Viklander, Maria, "Making Waves: Collaboration in the time of SARS-CoV-2 - rapid development of an international co-operation and wastewater surveillance database to support public health decision-making" in Water Research, 199 (2021):117167,
https://doi.org/10.1016/j.watres.2021.117167 . .

TY  - JOUR
AU  - Atanacković, Ana
AU  - Šporka, Ferdinand
AU  - Marković, Vanja
AU  - Slobodnik, Jaroslav
AU  - Zorić, Katarina
AU  - Csányi, Bela
AU  - Paunović, Momir
PY  - 2020
UR  - https://www.mdpi.com/2073-4441/12/9/2612
UR  - https://radar.ibiss.bg.ac.rs/123456789/3888
AB  - In this study, we analyzed the impacts of different environmental conditions on aquatic
worm communities along the Danube River, based on two longitudinal surveys, the Joint Danube
Surveys 2 and 3 (JDS; 2007 and 2013). We identified the most important environmental factors
(among analyzed groups) that shape worm communities: hydromorphlogical alterations, flow
velocity and substrate (HYMO group), dissolved oxygen, nitrates and nitrites (physico-chemical
parameters), zinc and nickel (metals), monobutyltin cation, benzo(b) fluoranthene and
benzo(k)fluoranthene, polychlorinated biphenyls PCB 77 and PCB 118 (selected chemical
determinants—organotin compounds, Polycyclic aromatic hydrocarbons—PAHs and PCBs). A
homogenization of species composition of Oligochaeta assemblages along the Danube was
confirmed. As one of main factors related to biotic homogenization, hydromorphological
alterations represented by similar changes in flow velocity and substrates along Danube’s course
could be singled out. Our results indicate that Oligochaeta could be used for the identification of
the level of hydromorphological degradation in large rivers (homogenization), rather than for
stressors classified as nutrient and organic pollutants. Our results provide additional evidence in
risk assessment of the environment, contributing in water management and monitoring of the
ecological status as proposed by the Water Framework Directive.
PB  - MDPI
T2  - Water
T1  - Aquatic Worm Assemblages along the Danube: A Homogenization Warning
IS  - 9
VL  - 12
DO  - 10.3390/w12092612
SP  - 2612
ER  - 

@article{
author = "Atanacković, Ana and Šporka, Ferdinand and Marković, Vanja and Slobodnik, Jaroslav and Zorić, Katarina and Csányi, Bela and Paunović, Momir",
year = "2020",
abstract = "In this study, we analyzed the impacts of different environmental conditions on aquatic
worm communities along the Danube River, based on two longitudinal surveys, the Joint Danube
Surveys 2 and 3 (JDS; 2007 and 2013). We identified the most important environmental factors
(among analyzed groups) that shape worm communities: hydromorphlogical alterations, flow
velocity and substrate (HYMO group), dissolved oxygen, nitrates and nitrites (physico-chemical
parameters), zinc and nickel (metals), monobutyltin cation, benzo(b) fluoranthene and
benzo(k)fluoranthene, polychlorinated biphenyls PCB 77 and PCB 118 (selected chemical
determinants—organotin compounds, Polycyclic aromatic hydrocarbons—PAHs and PCBs). A
homogenization of species composition of Oligochaeta assemblages along the Danube was
confirmed. As one of main factors related to biotic homogenization, hydromorphological
alterations represented by similar changes in flow velocity and substrates along Danube’s course
could be singled out. Our results indicate that Oligochaeta could be used for the identification of
the level of hydromorphological degradation in large rivers (homogenization), rather than for
stressors classified as nutrient and organic pollutants. Our results provide additional evidence in
risk assessment of the environment, contributing in water management and monitoring of the
ecological status as proposed by the Water Framework Directive.",
publisher = "MDPI",
journal = "Water",
title = "Aquatic Worm Assemblages along the Danube: A Homogenization Warning",
number = "9",
volume = "12",
doi = "10.3390/w12092612",
pages = "2612"
}

Atanacković, A., Šporka, F., Marković, V., Slobodnik, J., Zorić, K., Csányi, B.,& Paunović, M.. (2020). Aquatic Worm Assemblages along the Danube: A Homogenization Warning. in Water
MDPI., 12(9), 2612.
https://doi.org/10.3390/w12092612

Atanacković A, Šporka F, Marković V, Slobodnik J, Zorić K, Csányi B, Paunović M. Aquatic Worm Assemblages along the Danube: A Homogenization Warning. in Water. 2020;12(9):2612.
doi:10.3390/w12092612 .

Atanacković, Ana, Šporka, Ferdinand, Marković, Vanja, Slobodnik, Jaroslav, Zorić, Katarina, Csányi, Bela, Paunović, Momir, "Aquatic Worm Assemblages along the Danube: A Homogenization Warning" in Water, 12, no. 9 (2020):2612,
https://doi.org/10.3390/w12092612 . .

TY  - JOUR
AU  - Popović, Nataša
AU  - Raković, Maja
AU  - Đuknić, Jelena
AU  - Csányi, Béla
AU  - Szekeres, József
AU  - Borza, Péter
AU  - Slobodnik, Jaroslav
AU  - Liška, Igor
AU  - Milošević, Đurađ
AU  - Kolarević, Stoimir
AU  - Simić, Vladica
AU  - Tubić, Bojana
AU  - Paunović, Momir
PY  - 2020
UR  - https://jlimnol.it/index.php/jlimnol/article/view/jlimnol.2019.1915
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3553
AB  - This study was carried out to identify the relations between macroinvertebrate communities and river basin specific (RBS) pollutants in the Danube River. The investigation was performed at 68 sites along 2,500 km of the Danube. Forward selection (FS), canonical correspondence analyses (CCA), the Spearman correlation coefficient (SC) and BIO-ENV analysis (to detect synergistic effects) were used to identify the relations between the macroinvertebrate dataset and selected biological metrics with RBS pollutants. Of the 20 analysed pollutants (preselected based on NORMAN network methodology), seven (2,4-dinitrophenol, chloroxuron, bromacil, dimefuron, amoxicillin, bentazon and fluoranthene) were found to significantly correlate with macroinvertebrate communities. BIO-ENV analysis revealed 3 subsets of environmental variables that were in high correlation with the biota resemblance matrix, consisting mainly of a combination of the above-mentioned pollutants. Our results indicate that there are significant correlations between chemical determinants and aquatic biota. Moreover, this study contributes to the validation of the methodology used for prioritization of RBS pollutants proposed by the NORMAN network.
T2  - Journal of Limnology
T1  - The relationship between river basin specific (RBS) pollutants and macroinvertebrate communities
IS  - 1
VL  - 79
DO  - 10.4081/jlimnol.2019.1915
SP  - 59
EP  - 69
ER  - 

@article{
author = "Popović, Nataša and Raković, Maja and Đuknić, Jelena and Csányi, Béla and Szekeres, József and Borza, Péter and Slobodnik, Jaroslav and Liška, Igor and Milošević, Đurađ and Kolarević, Stoimir and Simić, Vladica and Tubić, Bojana and Paunović, Momir",
year = "2020",
abstract = "This study was carried out to identify the relations between macroinvertebrate communities and river basin specific (RBS) pollutants in the Danube River. The investigation was performed at 68 sites along 2,500 km of the Danube. Forward selection (FS), canonical correspondence analyses (CCA), the Spearman correlation coefficient (SC) and BIO-ENV analysis (to detect synergistic effects) were used to identify the relations between the macroinvertebrate dataset and selected biological metrics with RBS pollutants. Of the 20 analysed pollutants (preselected based on NORMAN network methodology), seven (2,4-dinitrophenol, chloroxuron, bromacil, dimefuron, amoxicillin, bentazon and fluoranthene) were found to significantly correlate with macroinvertebrate communities. BIO-ENV analysis revealed 3 subsets of environmental variables that were in high correlation with the biota resemblance matrix, consisting mainly of a combination of the above-mentioned pollutants. Our results indicate that there are significant correlations between chemical determinants and aquatic biota. Moreover, this study contributes to the validation of the methodology used for prioritization of RBS pollutants proposed by the NORMAN network.",
journal = "Journal of Limnology",
title = "The relationship between river basin specific (RBS) pollutants and macroinvertebrate communities",
number = "1",
volume = "79",
doi = "10.4081/jlimnol.2019.1915",
pages = "59-69"
}

Popović, N., Raković, M., Đuknić, J., Csányi, B., Szekeres, J., Borza, P., Slobodnik, J., Liška, I., Milošević, Đ., Kolarević, S., Simić, V., Tubić, B.,& Paunović, M.. (2020). The relationship between river basin specific (RBS) pollutants and macroinvertebrate communities. in Journal of Limnology, 79(1), 59-69.
https://doi.org/10.4081/jlimnol.2019.1915

Popović N, Raković M, Đuknić J, Csányi B, Szekeres J, Borza P, Slobodnik J, Liška I, Milošević Đ, Kolarević S, Simić V, Tubić B, Paunović M. The relationship between river basin specific (RBS) pollutants and macroinvertebrate communities. in Journal of Limnology. 2020;79(1):59-69.
doi:10.4081/jlimnol.2019.1915 .

Popović, Nataša, Raković, Maja, Đuknić, Jelena, Csányi, Béla, Szekeres, József, Borza, Péter, Slobodnik, Jaroslav, Liška, Igor, Milošević, Đurađ, Kolarević, Stoimir, Simić, Vladica, Tubić, Bojana, Paunović, Momir, "The relationship between river basin specific (RBS) pollutants and macroinvertebrate communities" in Journal of Limnology, 79, no. 1 (2020):59-69,
https://doi.org/10.4081/jlimnol.2019.1915 . .

TY  - CONF
AU  - Tubić, Bojana
AU  - Popović, Nataša
AU  - Atanacković, Ana
AU  - Petrović, Ana
AU  - Simić, Vladica
AU  - Csanyi, Bela
AU  - Szekeres, J.
AU  - Borza, P.
AU  - Slobodnik, Jaroslav
AU  - Liška, Igor
AU  - Milošević, Đurađ
AU  - Kolarević, Stoimir
AU  - Paunović, Momir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4843
AB  - This paper discusses the use of different methods (multihabitat - MSH, Kick & Sweep 
method, Air lift sampling, multcorer, Van Veen grab, dredging) for aquatic 
macroinvertebrates sampling. Material was collected from the Danube River as a part of the 
three research programs: Joint Danube Survey 2, performed in period August-September 
2007 from Regensburg to Danube delta (96 sampling sites), Joint Danube Survey 3, 
performed in period August-September 2007 (68 sites) and national monitoring program 
performed in period September-November 2014 along Serbian stretch of the Danube River 
(12 sites). The aim of the study was to contribute to the standardization of the sampling 
procedures for the large lowland rivers. We tested the effectiveness of sampling methods in 
respect to macroinvertebrate taxa richness and metrics typically used for ecological status 
assessment. The selection of sampling approach is also discussed in relation to objectives of 
the survey – e.g. research, or routine monitoring, as well in respect to target taxa. Based on 
the our results we concluded that it is necessary to employ more than one sampling 
technique and to cover all available regions of large rivers, including the deeper parts in the 
case of research programs. For the routine monitoring we recommend to use standardized 
K&S method. Studies of the specific group of organisms require detailed monitoring of 
available habitats, and use of additional sampling methods (e.g. mussels or crayfish studies).
PB  - Zagreb: Croatian Biological Society
C3  - Book of abstracts, 13. Croatian biological congress with international participation, 19 - 23. September 2018, Poreč, Croatia
T1  - Standardising the methods for sampling aquatic macroinvertebrates of the large lowland rivers
SP  - 233
EP  - 234
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4843
ER  - 

@conference{
author = "Tubić, Bojana and Popović, Nataša and Atanacković, Ana and Petrović, Ana and Simić, Vladica and Csanyi, Bela and Szekeres, J. and Borza, P. and Slobodnik, Jaroslav and Liška, Igor and Milošević, Đurađ and Kolarević, Stoimir and Paunović, Momir",
year = "2018",
abstract = "This paper discusses the use of different methods (multihabitat - MSH, Kick & Sweep 
method, Air lift sampling, multcorer, Van Veen grab, dredging) for aquatic 
macroinvertebrates sampling. Material was collected from the Danube River as a part of the 
three research programs: Joint Danube Survey 2, performed in period August-September 
2007 from Regensburg to Danube delta (96 sampling sites), Joint Danube Survey 3, 
performed in period August-September 2007 (68 sites) and national monitoring program 
performed in period September-November 2014 along Serbian stretch of the Danube River 
(12 sites). The aim of the study was to contribute to the standardization of the sampling 
procedures for the large lowland rivers. We tested the effectiveness of sampling methods in 
respect to macroinvertebrate taxa richness and metrics typically used for ecological status 
assessment. The selection of sampling approach is also discussed in relation to objectives of 
the survey – e.g. research, or routine monitoring, as well in respect to target taxa. Based on 
the our results we concluded that it is necessary to employ more than one sampling 
technique and to cover all available regions of large rivers, including the deeper parts in the 
case of research programs. For the routine monitoring we recommend to use standardized 
K&S method. Studies of the specific group of organisms require detailed monitoring of 
available habitats, and use of additional sampling methods (e.g. mussels or crayfish studies).",
publisher = "Zagreb: Croatian Biological Society",
journal = "Book of abstracts, 13. Croatian biological congress with international participation, 19 - 23. September 2018, Poreč, Croatia",
title = "Standardising the methods for sampling aquatic macroinvertebrates of the large lowland rivers",
pages = "233-234",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4843"
}

Tubić, B., Popović, N., Atanacković, A., Petrović, A., Simić, V., Csanyi, B., Szekeres, J., Borza, P., Slobodnik, J., Liška, I., Milošević, Đ., Kolarević, S.,& Paunović, M.. (2018). Standardising the methods for sampling aquatic macroinvertebrates of the large lowland rivers. in Book of abstracts, 13. Croatian biological congress with international participation, 19 - 23. September 2018, Poreč, Croatia
Zagreb: Croatian Biological Society., 233-234.
https://hdl.handle.net/21.15107/rcub_ibiss_4843

Tubić B, Popović N, Atanacković A, Petrović A, Simić V, Csanyi B, Szekeres J, Borza P, Slobodnik J, Liška I, Milošević Đ, Kolarević S, Paunović M. Standardising the methods for sampling aquatic macroinvertebrates of the large lowland rivers. in Book of abstracts, 13. Croatian biological congress with international participation, 19 - 23. September 2018, Poreč, Croatia. 2018;:233-234.
https://hdl.handle.net/21.15107/rcub_ibiss_4843 .

Tubić, Bojana, Popović, Nataša, Atanacković, Ana, Petrović, Ana, Simić, Vladica, Csanyi, Bela, Szekeres, J., Borza, P., Slobodnik, Jaroslav, Liška, Igor, Milošević, Đurađ, Kolarević, Stoimir, Paunović, Momir, "Standardising the methods for sampling aquatic macroinvertebrates of the large lowland rivers" in Book of abstracts, 13. Croatian biological congress with international participation, 19 - 23. September 2018, Poreč, Croatia (2018):233-234,
https://hdl.handle.net/21.15107/rcub_ibiss_4843 .

TY  - CONF
AU  - Popović, Nataša
AU  - Tubić, Bojana
AU  - Raković, Maja
AU  - Marinković, Nikola
AU  - Csányi, Bela
AU  - Szekeres, Jozsef
AU  - Borza, Peter
AU  - Slobodnik, Jaroslav
AU  - Liška, Igor
AU  - Milošević, Đurađ
AU  - Kolarević, Stoimir
AU  - Ilić, Marija
AU  - Mitrović, Miroslava
AU  - Paunović, Momir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4841
AB  - This study was carried out to identify relations between the macroinvertebrate communities and 
River Basin Specific pollutants (RBS pollutants) in the Danube River. The investigation was 
performed on 68 sites along 2,500km of the Danube. Forward Selection (FS) method, Canonical 
Correspondence Analyses (CCA) and the Spearman correlation coefficient (SC) were used to 
identify the relations between macroinvertebrate dataset and selected biological metrics and 
RBS pollutants. Out of 20 analysed pollutants, seven (2,4-Dinitrophenol, Chloroxuron, 
Bromacil, Dimefuron, Amoxicillin, Bentazon and Fluoranthene) were found to be significantly 
correlated with macroinvertebrate communities. Bentazon, Dimefuron, 2,4-Dinitrophenol, 
Fluoranthene and Chloroxuron showed negative correlation with total number of taxa and 
abundance of Trichoptera, Diptera, Oligochaeta, Crustacea and Odonata, as well as abundance 
of Gastropoda and total number of taxa. To find synergistic influence of RBS pollutants on 
biota, BIO-ENV analysis was performed and reviled that 3 subsets of environmental variables 
were highly correlated with biota resemblance matrix, covering combination of same 
parameters singled out by FA.
PB  - Łódź: Department of Invertebrate Zoology & Hydrobiology University of Łódź
C3  - Abstracts of the 3rd CESAMIR, Central Europena Symposium for Aquatic Macroinvertebrates Research; 2018 July 8-13; Lódź, Poland
T1  - Aquatic macroinvertebrate community patterns as a tool prioritization of River Basin  Specific Pollutants
SP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4841
ER  - 

@conference{
author = "Popović, Nataša and Tubić, Bojana and Raković, Maja and Marinković, Nikola and Csányi, Bela and Szekeres, Jozsef and Borza, Peter and Slobodnik, Jaroslav and Liška, Igor and Milošević, Đurađ and Kolarević, Stoimir and Ilić, Marija and Mitrović, Miroslava and Paunović, Momir",
year = "2018",
abstract = "This study was carried out to identify relations between the macroinvertebrate communities and 
River Basin Specific pollutants (RBS pollutants) in the Danube River. The investigation was 
performed on 68 sites along 2,500km of the Danube. Forward Selection (FS) method, Canonical 
Correspondence Analyses (CCA) and the Spearman correlation coefficient (SC) were used to 
identify the relations between macroinvertebrate dataset and selected biological metrics and 
RBS pollutants. Out of 20 analysed pollutants, seven (2,4-Dinitrophenol, Chloroxuron, 
Bromacil, Dimefuron, Amoxicillin, Bentazon and Fluoranthene) were found to be significantly 
correlated with macroinvertebrate communities. Bentazon, Dimefuron, 2,4-Dinitrophenol, 
Fluoranthene and Chloroxuron showed negative correlation with total number of taxa and 
abundance of Trichoptera, Diptera, Oligochaeta, Crustacea and Odonata, as well as abundance 
of Gastropoda and total number of taxa. To find synergistic influence of RBS pollutants on 
biota, BIO-ENV analysis was performed and reviled that 3 subsets of environmental variables 
were highly correlated with biota resemblance matrix, covering combination of same 
parameters singled out by FA.",
publisher = "Łódź: Department of Invertebrate Zoology & Hydrobiology University of Łódź",
journal = "Abstracts of the 3rd CESAMIR, Central Europena Symposium for Aquatic Macroinvertebrates Research; 2018 July 8-13; Lódź, Poland",
title = "Aquatic macroinvertebrate community patterns as a tool prioritization of River Basin  Specific Pollutants",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4841"
}

Popović, N., Tubić, B., Raković, M., Marinković, N., Csányi, B., Szekeres, J., Borza, P., Slobodnik, J., Liška, I., Milošević, Đ., Kolarević, S., Ilić, M., Mitrović, M.,& Paunović, M.. (2018). Aquatic macroinvertebrate community patterns as a tool prioritization of River Basin  Specific Pollutants. in Abstracts of the 3rd CESAMIR, Central Europena Symposium for Aquatic Macroinvertebrates Research; 2018 July 8-13; Lódź, Poland
Łódź: Department of Invertebrate Zoology & Hydrobiology University of Łódź., 54.
https://hdl.handle.net/21.15107/rcub_ibiss_4841

Popović N, Tubić B, Raković M, Marinković N, Csányi B, Szekeres J, Borza P, Slobodnik J, Liška I, Milošević Đ, Kolarević S, Ilić M, Mitrović M, Paunović M. Aquatic macroinvertebrate community patterns as a tool prioritization of River Basin  Specific Pollutants. in Abstracts of the 3rd CESAMIR, Central Europena Symposium for Aquatic Macroinvertebrates Research; 2018 July 8-13; Lódź, Poland. 2018;:54.
https://hdl.handle.net/21.15107/rcub_ibiss_4841 .

Popović, Nataša, Tubić, Bojana, Raković, Maja, Marinković, Nikola, Csányi, Bela, Szekeres, Jozsef, Borza, Peter, Slobodnik, Jaroslav, Liška, Igor, Milošević, Đurađ, Kolarević, Stoimir, Ilić, Marija, Mitrović, Miroslava, Paunović, Momir, "Aquatic macroinvertebrate community patterns as a tool prioritization of River Basin  Specific Pollutants" in Abstracts of the 3rd CESAMIR, Central Europena Symposium for Aquatic Macroinvertebrates Research; 2018 July 8-13; Lódź, Poland (2018):54,
https://hdl.handle.net/21.15107/rcub_ibiss_4841 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Slobodnik, Jaroslav
AU  - Liška, Igor
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Knežević-Vukčević, Jelena
AU  - Vuković-Gačić, Branka
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969715302576
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84945474289&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2913
AB  - In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 - the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker - DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).
PB  - Elsevier
T2  - Science of The Total Environment
T2  - Science of the Total Environment
T1  - Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3
VL  - 540
DO  - 10.1016/j.scitotenv.2015.06.061
SP  - 377
EP  - 385
ER  - 

@article{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kostić, Jovana and Slobodnik, Jaroslav and Liška, Igor and Gačić, Zoran and Paunović, Momir and Knežević-Vukčević, Jelena and Vuković-Gačić, Branka",
year = "2016",
abstract = "In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 - the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker - DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).",
publisher = "Elsevier",
journal = "Science of The Total Environment, Science of the Total Environment",
title = "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3",
volume = "540",
doi = "10.1016/j.scitotenv.2015.06.061",
pages = "377-385"
}

Kolarević, S., Kračun-Kolarević, M., Kostić, J., Slobodnik, J., Liška, I., Gačić, Z., Paunović, M., Knežević-Vukčević, J.,& Vuković-Gačić, B.. (2016). Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of The Total Environment
Elsevier., 540, 377-385.
https://doi.org/10.1016/j.scitotenv.2015.06.061

Kolarević S, Kračun-Kolarević M, Kostić J, Slobodnik J, Liška I, Gačić Z, Paunović M, Knežević-Vukčević J, Vuković-Gačić B. Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of The Total Environment. 2016;540:377-385.
doi:10.1016/j.scitotenv.2015.06.061 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kostić, Jovana, Slobodnik, Jaroslav, Liška, Igor, Gačić, Zoran, Paunović, Momir, Knežević-Vukčević, Jelena, Vuković-Gačić, Branka, "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3" in Science of The Total Environment, 540 (2016):377-385,
https://doi.org/10.1016/j.scitotenv.2015.06.061 . .

TY  - JOUR
AU  - Deutschmann, Björn
AU  - Kolarević, Stoimir
AU  - Brack, Werner
AU  - Kaisarević, Sonja
AU  - Kostić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Liska, Igor
AU  - Paunović, Momir
AU  - Seiler, Thomas-Benjamin
AU  - Shao, Ying
AU  - Sipos, Sandor
AU  - Slobodnik, Jaroslav
AU  - Teodorović, Ivana
AU  - Vuković-Gačić, Branka
AU  - Hollert, Henner
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969716316308
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2533
AB  - The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the . in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both . in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.
T2  - Science of The Total Environment
T1  - Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.175
SP  - 1441
EP  - 1449
ER  - 

@article{
author = "Deutschmann, Björn and Kolarević, Stoimir and Brack, Werner and Kaisarević, Sonja and Kostić, Jovana and Kračun-Kolarević, Margareta and Liska, Igor and Paunović, Momir and Seiler, Thomas-Benjamin and Shao, Ying and Sipos, Sandor and Slobodnik, Jaroslav and Teodorović, Ivana and Vuković-Gačić, Branka and Hollert, Henner",
year = "2016",
abstract = "The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the . in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both . in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.",
journal = "Science of The Total Environment",
title = "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.175",
pages = "1441-1449"
}

Deutschmann, B., Kolarević, S., Brack, W., Kaisarević, S., Kostić, J., Kračun-Kolarević, M., Liska, I., Paunović, M., Seiler, T., Shao, Y., Sipos, S., Slobodnik, J., Teodorović, I., Vuković-Gačić, B.,& Hollert, H.. (2016). Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of The Total Environment, 573, 1441-1449.
https://doi.org/10.1016/j.scitotenv.2016.07.175

Deutschmann B, Kolarević S, Brack W, Kaisarević S, Kostić J, Kračun-Kolarević M, Liska I, Paunović M, Seiler T, Shao Y, Sipos S, Slobodnik J, Teodorović I, Vuković-Gačić B, Hollert H. Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of The Total Environment. 2016;573:1441-1449.
doi:10.1016/j.scitotenv.2016.07.175 .

Deutschmann, Björn, Kolarević, Stoimir, Brack, Werner, Kaisarević, Sonja, Kostić, Jovana, Kračun-Kolarević, Margareta, Liska, Igor, Paunović, Momir, Seiler, Thomas-Benjamin, Shao, Ying, Sipos, Sandor, Slobodnik, Jaroslav, Teodorović, Ivana, Vuković-Gačić, Branka, Hollert, Henner, "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay" in Science of The Total Environment, 573 (2016):1441-1449,
https://doi.org/10.1016/j.scitotenv.2016.07.175 . .