TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Pavlović, Kasja
AU  - Krako Jakoviljević, Nina
AU  - Ivanović, Tijana
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Đorđević, Mirko
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6252
AB  - Evolutionary theory suggests that inheritance of mitochondria through the maternal lineage could drive the accumulation of male-harming mutations in mitochondrial genomes. Namely, due to uniparental mode of mitochondrial inheritance, natural selection is “blind” to mutations that are deleterious to males as long as they are beneficial or neutral to females. This evolutionary phenomenon is known as ‘the Mother’s Curse’. The male-specific adverse effects of mitochondrial mutations are thought to be due to the fact that males are sensitive to impairments in the function of the oxidative phosphorylation (OXPHOS) system, i.e. mitochondrial respiration. The OXPHOS system consists of five complexes whose subunits are coded by the mitochondrial and nuclear genomes. In our previous work, we identified several mitochondrial haplotypes (mitotypes) in laboratory populations of the seed beetle (Acanthoscelides obtectus). One of them, MG3b mitotype, significantly reduces male fertility compared to other mitotypes when expressed in the same nuclear environment, but has no negative effects on females. In this study, we tested whether the MG3b mitotype affects OXPHOS functioning in males compared with other, control mitotypes. Specifically, we expressed MG3b and two control mitotypes alongside the same nuclear background and measured mitochondrial respiration in males using high-resolution respirometry. Our results show that respiration linked to all OXPHOS complexes is significantly reduced in MG3b males compared with controls. This is particularly true for the complex IV, which shows mean reduction in activity of around 40%. This work provides evidence that the decrease in mitochondrial respiration is the cause of subfertility of MG3b males.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6252
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Pavlović, Kasja and Krako Jakoviljević, Nina and Ivanović, Tijana and Budečević, Sanja and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Đorđević, Mirko",
year = "2023",
abstract = "Evolutionary theory suggests that inheritance of mitochondria through the maternal lineage could drive the accumulation of male-harming mutations in mitochondrial genomes. Namely, due to uniparental mode of mitochondrial inheritance, natural selection is “blind” to mutations that are deleterious to males as long as they are beneficial or neutral to females. This evolutionary phenomenon is known as ‘the Mother’s Curse’. The male-specific adverse effects of mitochondrial mutations are thought to be due to the fact that males are sensitive to impairments in the function of the oxidative phosphorylation (OXPHOS) system, i.e. mitochondrial respiration. The OXPHOS system consists of five complexes whose subunits are coded by the mitochondrial and nuclear genomes. In our previous work, we identified several mitochondrial haplotypes (mitotypes) in laboratory populations of the seed beetle (Acanthoscelides obtectus). One of them, MG3b mitotype, significantly reduces male fertility compared to other mitotypes when expressed in the same nuclear environment, but has no negative effects on females. In this study, we tested whether the MG3b mitotype affects OXPHOS functioning in males compared with other, control mitotypes. Specifically, we expressed MG3b and two control mitotypes alongside the same nuclear background and measured mitochondrial respiration in males using high-resolution respirometry. Our results show that respiration linked to all OXPHOS complexes is significantly reduced in MG3b males compared with controls. This is particularly true for the complex IV, which shows mean reduction in activity of around 40%. This work provides evidence that the decrease in mitochondrial respiration is the cause of subfertility of MG3b males.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6252"
}

Vlajnić, L., Savković, U., Pavlović, K., Krako Jakoviljević, N., Ivanović, T., Budečević, S., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Đorđević, M.. (2023). Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 40.
https://hdl.handle.net/21.15107/rcub_ibiss_6252

Vlajnić L, Savković U, Pavlović K, Krako Jakoviljević N, Ivanović T, Budečević S, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Đorđević M. Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:40.
https://hdl.handle.net/21.15107/rcub_ibiss_6252 .

Vlajnić, Lea, Savković, Uroš, Pavlović, Kasja, Krako Jakoviljević, Nina, Ivanović, Tijana, Budečević, Sanja, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Đorđević, Mirko, "Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):40,
https://hdl.handle.net/21.15107/rcub_ibiss_6252 .

TY  - CONF
AU  - Budečević, Sanja
AU  - Rončević, Aleksa
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Savković, Uroš
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6254
AB  - Host-shift induced stress in phytophagous insects could have effect on developmental destabilization. Indicator of developmental instability is fluctuating asymmetry (FA)- small random deviations between right and left side of bilateral symmetrical traits. In theory, symmetry is subject to sexual selection in insects, since FA is considered as an indicator of male quality. Response to environmental variation is commonly sex-specific and can affect patterns of sexual dimorphism. We performed reciprocal transplant experiment in eight replicated seed beetle (Acanthoscelides obtectus) populations adapted to bean and chickpea. Within the framework of experimental evolution and using the method of geometric morphometrics we: 1. observed the effects of long and short term host-shift on patterns of shape variation of beetles of both sexes and 2. tested hypothesis that both sexes will have a higher level of FA after long or short term change from optimal to suboptimal host. We found that long term host-shift process affected shape in a way that bean adapted beetles had wider and shorter abdomen compared to chickpea adapted ones. Short-term effects are more notable in bean selected beetles, especially in the abdominal region. Bean adapted males had the most canalized development, i.e. the lowest level of FA, compared to chickpea beetles. In chickpea adapted females, difference in level of FA significantly decreased after the short term host-shift. Our work indicates that host-shift process can change morphological aspects of beetles in a sex-specific manner and consequently influence their developmental trajectories.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach
SP  - 362
EP  - 363
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6254
ER  - 

@conference{
author = "Budečević, Sanja and Rončević, Aleksa and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Savković, Uroš",
year = "2023",
abstract = "Host-shift induced stress in phytophagous insects could have effect on developmental destabilization. Indicator of developmental instability is fluctuating asymmetry (FA)- small random deviations between right and left side of bilateral symmetrical traits. In theory, symmetry is subject to sexual selection in insects, since FA is considered as an indicator of male quality. Response to environmental variation is commonly sex-specific and can affect patterns of sexual dimorphism. We performed reciprocal transplant experiment in eight replicated seed beetle (Acanthoscelides obtectus) populations adapted to bean and chickpea. Within the framework of experimental evolution and using the method of geometric morphometrics we: 1. observed the effects of long and short term host-shift on patterns of shape variation of beetles of both sexes and 2. tested hypothesis that both sexes will have a higher level of FA after long or short term change from optimal to suboptimal host. We found that long term host-shift process affected shape in a way that bean adapted beetles had wider and shorter abdomen compared to chickpea adapted ones. Short-term effects are more notable in bean selected beetles, especially in the abdominal region. Bean adapted males had the most canalized development, i.e. the lowest level of FA, compared to chickpea beetles. In chickpea adapted females, difference in level of FA significantly decreased after the short term host-shift. Our work indicates that host-shift process can change morphological aspects of beetles in a sex-specific manner and consequently influence their developmental trajectories.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach",
pages = "362-363",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6254"
}

Budečević, S., Rončević, A., Đorđević, M., Vlajnić, L., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Savković, U.. (2023). Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 362-363.
https://hdl.handle.net/21.15107/rcub_ibiss_6254

Budečević S, Rončević A, Đorđević M, Vlajnić L, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Savković U. Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:362-363.
https://hdl.handle.net/21.15107/rcub_ibiss_6254 .

Budečević, Sanja, Rončević, Aleksa, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Savković, Uroš, "Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):362-363,
https://hdl.handle.net/21.15107/rcub_ibiss_6254 .

TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Vukajlović, Filip
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Stojković, Oliver
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6017
AB  - This data set is a milestone  of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains raw data on the effects of candidate Trojan female technique (TFT) mutations across the range of nuclear backgrounds on fertility of male and female seed beetles. For obtaining this data set we have used controlled genetic crosses between females with 3 specific mitotypes (1 of which is a candidate TFT mitotype, and 2 are controls) and males with 5 different nuclear backgrounds. Such crossings were used to assess female and male fertility of seed beetles with different combinations of mito-nuclear genomes.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6017
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Vukajlović, Filip and Budečević, Sanja and Stojković, Biljana and Stojković, Oliver and Pešić, Snežana and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2023",
abstract = "This data set is a milestone  of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains raw data on the effects of candidate Trojan female technique (TFT) mutations across the range of nuclear backgrounds on fertility of male and female seed beetles. For obtaining this data set we have used controlled genetic crosses between females with 3 specific mitotypes (1 of which is a candidate TFT mitotype, and 2 are controls) and males with 5 different nuclear backgrounds. Such crossings were used to assess female and male fertility of seed beetles with different combinations of mito-nuclear genomes.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6017"
}

Vlajnić, L., Savković, U., Vukajlović, F., Budečević, S., Stojković, B., Stojković, O., Pešić, S., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2023). Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6017

Vlajnić L, Savković U, Vukajlović F, Budečević S, Stojković B, Stojković O, Pešić S, Predojević D, Mitrovski Bogdanović A, Đorđević M. Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6017 .

Vlajnić, Lea, Savković, Uroš, Vukajlović, Filip, Budečević, Sanja, Stojković, Biljana, Stojković, Oliver, Pešić, Snežana, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6017 .

TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5031
AB  - Uniparental inheritance of mitochondria leads to asymmetry of mtDNA evolution because
selection acts directly on non-neutral mtDNA polymorphisms only through the female
lineage. In other words, natural selection will not recognize any mtDNA mutation which is
male harming, but neutral, beneficial, or slightly deleterious for females. Ultimately, non-
Mendelian inheritance of mitochondria drives the evolution of male-specific mitochondrial
mutation loads, an idea known as mother’s curse. Naturally occurring mtDNA mutations
that impair male fertility, but have no effects on females, have been recognized as an
opportunity for the development of the biocontrol of pest species termed Trojan Female
Technique (TFT). Namely, females carrying TFT mutations, and their female
descendants, could continuously, over multiple generations, produce males that sire
fewer offspring than wild-type counterparts. Although the TFT shows promise as a transgenerational,
self-sustaining method, its empirical success hinges on the existence of
natural TFT mutations that remain uncharacterized in pest insects. In order to identify
and test possible TFT candidates for biological control of seed beetle, a common pest in
legume storages, three mtDNA haplotypes found in laboratory lines were expressed
alongside one outbred nuclear background. We analyzed the effects of specific
mitotypes on female and male life history traits, and found a male-only reduction in
fertility in one mito-nuclear combination. Specifically, fertility (but not total fecundity and
lifespan) of males carrying specific mtDNA variant was reduced by 35% relative to the
controls. Our study provides evidence that intergenomic conflict leads to the existence of
male-harming mtDNA mutations that segregate within populations.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus
SP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5031
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2022",
abstract = "Uniparental inheritance of mitochondria leads to asymmetry of mtDNA evolution because
selection acts directly on non-neutral mtDNA polymorphisms only through the female
lineage. In other words, natural selection will not recognize any mtDNA mutation which is
male harming, but neutral, beneficial, or slightly deleterious for females. Ultimately, non-
Mendelian inheritance of mitochondria drives the evolution of male-specific mitochondrial
mutation loads, an idea known as mother’s curse. Naturally occurring mtDNA mutations
that impair male fertility, but have no effects on females, have been recognized as an
opportunity for the development of the biocontrol of pest species termed Trojan Female
Technique (TFT). Namely, females carrying TFT mutations, and their female
descendants, could continuously, over multiple generations, produce males that sire
fewer offspring than wild-type counterparts. Although the TFT shows promise as a transgenerational,
self-sustaining method, its empirical success hinges on the existence of
natural TFT mutations that remain uncharacterized in pest insects. In order to identify
and test possible TFT candidates for biological control of seed beetle, a common pest in
legume storages, three mtDNA haplotypes found in laboratory lines were expressed
alongside one outbred nuclear background. We analyzed the effects of specific
mitotypes on female and male life history traits, and found a male-only reduction in
fertility in one mito-nuclear combination. Specifically, fertility (but not total fecundity and
lifespan) of males carrying specific mtDNA variant was reduced by 35% relative to the
controls. Our study provides evidence that intergenomic conflict leads to the existence of
male-harming mtDNA mutations that segregate within populations.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus",
pages = "204",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5031"
}

Vlajnić, L., Savković, U., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2022). Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 204.
https://hdl.handle.net/21.15107/rcub_ibiss_5031

Vlajnić L, Savković U, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Đorđević M. Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:204.
https://hdl.handle.net/21.15107/rcub_ibiss_5031 .

Vlajnić, Lea, Savković, Uroš, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):204,
https://hdl.handle.net/21.15107/rcub_ibiss_5031 .