TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Dovenska, Monika
AU  - Popovska-Perčinić, Florina
PY  - 2022
UR  - https://www.sciendo.com/article/10.2478/acve-2022-0001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4965
AB  - Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.
T2  - Acta Veterinaria
T1  - The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study
IS  - 1
VL  - 72
DO  - 10.2478/acve-2022-0001
SP  - 1
EP  - 15
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Ajdžanović, Vladimir and Pendovski, Lazo and Ristić, Nataša and Milošević, Verica and Dovenska, Monika and Popovska-Perčinić, Florina",
year = "2022",
abstract = "Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.",
journal = "Acta Veterinaria",
title = "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0001",
pages = "1-15"
}

Čukuranović Kokoris, J., Ajdžanović, V., Pendovski, L., Ristić, N., Milošević, V., Dovenska, M.,& Popovska-Perčinić, F.. (2022). The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria, 72(1), 1-15.
https://doi.org/10.2478/acve-2022-0001

Čukuranović Kokoris J, Ajdžanović V, Pendovski L, Ristić N, Milošević V, Dovenska M, Popovska-Perčinić F. The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria. 2022;72(1):1-15.
doi:10.2478/acve-2022-0001 .

Čukuranović Kokoris, Jovana, Ajdžanović, Vladimir, Pendovski, Lazo, Ristić, Nataša, Milošević, Verica, Dovenska, Monika, Popovska-Perčinić, Florina, "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study" in Acta Veterinaria, 72, no. 1 (2022):1-15,
https://doi.org/10.2478/acve-2022-0001 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2021
UR  - http://www.publish.csiro.au/?paper=RD20164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4153
AB  - Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.
PB  - CSIRO
T2  - Reproduction, Fertility and Development
T1  - Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat
IS  - 3
VL  - 33
DO  - 10.1071/RD20164
SP  - 245
EP  - 255
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2021",
abstract = "Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.",
publisher = "CSIRO",
journal = "Reproduction, Fertility and Development",
title = "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat",
number = "3",
volume = "33",
doi = "10.1071/RD20164",
pages = "245-255"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2021). Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development
CSIRO., 33(3), 245-255.
https://doi.org/10.1071/RD20164

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development. 2021;33(3):245-255.
doi:10.1071/RD20164 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat" in Reproduction, Fertility and Development, 33, no. 3 (2021):245-255,
https://doi.org/10.1071/RD20164 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2021
UR  - http://www.publish.csiro.au/?paper=RD20164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4153
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4163
AB  - Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.
PB  - CSIRO
T2  - Reproduction, Fertility and Development
T1  - Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat
IS  - 3
VL  - 33
DO  - 10.1071/RD20164
SP  - 245
EP  - 255
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2021",
abstract = "Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.",
publisher = "CSIRO",
journal = "Reproduction, Fertility and Development",
title = "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat",
number = "3",
volume = "33",
doi = "10.1071/RD20164",
pages = "245-255"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2021). Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development
CSIRO., 33(3), 245-255.
https://doi.org/10.1071/RD20164

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development. 2021;33(3):245-255.
doi:10.1071/RD20164 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat" in Reproduction, Fertility and Development, 33, no. 3 (2021):245-255,
https://doi.org/10.1071/RD20164 . .

TY  - JOUR
AU  - Popovska-Perčinić, Florina
AU  - Manojlović-Stojanoski, Milica
AU  - Pendovski, Lazo
AU  - Dinevska Kjovkarovska, Suzana
AU  - Miova, Biljana
AU  - Grubin, Jasmina
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/123456789/3867
AB  - Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats.

Materials and methods: In this experimental study, the morpho-functional state of adrenal glands was estimated by stereological evaluation of parameters, including the adrenal volume, adrenocortical cell/nuclear size and number, and the volume density of vascular tissues after four days of exposure to a moderate increase in ambient temperature of 35 ± 1˚C. Novelli histochemical and vascular endothelial growth factor (VEGF) immunohistochemical staining provided insight into the adrenal gland vascular network. Additionally, the adrenal levels of aldosterone, corticosterone, and pituitary adrenocorticotropic hormone (ACTH) were determined as crucial indicators of the hypothalamic-pituitaryadrenocortical (HPA) axis activity.

Results: Prolonged exposure to a moderate increase in ambient temperature for four days resulted in a significant increase in ACTH level up to 24%, which altered adrenal glands both structurally and functionally. The adrenocortical volume and number of cells in all cortical zones were markedly increased (P<0.05). A statistically significant increase was shown in the level of aldosterone (16%) and corticosterone (25%) in serum levels of individuals.

Conclusion: Increased activity of the HPA axis reflects the response to a moderate increase in ambient temperature during four days, showing the capacity of the HPA axis to adapt the organism to daily temperature changes.
PB  - Royan Institute
T2  - Cell Journal
T1  - A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats
IS  - 4
VL  - 22
DO  - 10.22074/cellj.2021.6827
SP  - 415
EP  - 424
ER  - 

@article{
author = "Popovska-Perčinić, Florina and Manojlović-Stojanoski, Milica and Pendovski, Lazo and Dinevska Kjovkarovska, Suzana and Miova, Biljana and Grubin, Jasmina and Milošević, Verica and Ajdžanović, Vladimir",
year = "2021",
abstract = "Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats.

Materials and methods: In this experimental study, the morpho-functional state of adrenal glands was estimated by stereological evaluation of parameters, including the adrenal volume, adrenocortical cell/nuclear size and number, and the volume density of vascular tissues after four days of exposure to a moderate increase in ambient temperature of 35 ± 1˚C. Novelli histochemical and vascular endothelial growth factor (VEGF) immunohistochemical staining provided insight into the adrenal gland vascular network. Additionally, the adrenal levels of aldosterone, corticosterone, and pituitary adrenocorticotropic hormone (ACTH) were determined as crucial indicators of the hypothalamic-pituitaryadrenocortical (HPA) axis activity.

Results: Prolonged exposure to a moderate increase in ambient temperature for four days resulted in a significant increase in ACTH level up to 24%, which altered adrenal glands both structurally and functionally. The adrenocortical volume and number of cells in all cortical zones were markedly increased (P<0.05). A statistically significant increase was shown in the level of aldosterone (16%) and corticosterone (25%) in serum levels of individuals.

Conclusion: Increased activity of the HPA axis reflects the response to a moderate increase in ambient temperature during four days, showing the capacity of the HPA axis to adapt the organism to daily temperature changes.",
publisher = "Royan Institute",
journal = "Cell Journal",
title = "A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats",
number = "4",
volume = "22",
doi = "10.22074/cellj.2021.6827",
pages = "415-424"
}

Popovska-Perčinić, F., Manojlović-Stojanoski, M., Pendovski, L., Dinevska Kjovkarovska, S., Miova, B., Grubin, J., Milošević, V.,& Ajdžanović, V.. (2021). A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats. in Cell Journal
Royan Institute., 22(4), 415-424.
https://doi.org/10.22074/cellj.2021.6827

Popovska-Perčinić F, Manojlović-Stojanoski M, Pendovski L, Dinevska Kjovkarovska S, Miova B, Grubin J, Milošević V, Ajdžanović V. A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats. in Cell Journal. 2021;22(4):415-424.
doi:10.22074/cellj.2021.6827 .

Popovska-Perčinić, Florina, Manojlović-Stojanoski, Milica, Pendovski, Lazo, Dinevska Kjovkarovska, Suzana, Miova, Biljana, Grubin, Jasmina, Milošević, Verica, Ajdžanović, Vladimir, "A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats" in Cell Journal, 22, no. 4 (2021):415-424,
https://doi.org/10.22074/cellj.2021.6827 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2019
UR  - http://doi.wiley.com/10.1111/fcp.12415
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3155
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3442
AB  - Dexamethasone (Dx) is often used in obstetric practice to promote fetal lung maturation and to prevent respiratory distress syndrome when the risk of preterm delivery persists. This therapy enables survival of the newborn, but also is associated with deleterious effects on the offspring, such as reproductive disorders. The aim of this study was to determine specifically whether prenatal exposure to Dx disturbs the physiological balance between proliferation and apoptosis of germinative cells (GC) in the ovary of 19 and 21 day old fetuses and thus induces developmental programing of the female reproductive system. Pregnant Wistar rats (n = 10/group), separated into control (vehicle) and Dx-treated (0.5 mg/kg body mass) groups, received injections on gestational days 16, 17 and 18. Exposure to Dx lowered the volume of the fetal ovary by 30% (p<0.05) in 21 day old fetuses, as well as the total number of GC in the ovary by 21% (p<0.05). When compared to the controls, in Dx-exposed fetuses the total number of PCNA positive GC was 27% lower at 19 days and 71% lower at 21 day old (p<0.05), while total numbers of caspase-3 positive GC were 2.3 fold and 34% higher respectively, (p<0.05). Our results demonstrate that prenatal exposure to Dx diminished proliferation but increased the rate of germinative cell apoptosis, with consequently reduced total germinative cell number and ovary volume. Impairment of fetal oogenesis and fewer GC in the fetal ovary compromise the oogonial stock and thus may constitute a risk for female fertility. This article is protected by copyright. All rights reserved.
T2  - Fundamental & Clinical Pharmacology
T1  - Adverse effect of dexamethasone on development of the fetal rat ovary.
IS  - 2
VL  - 33
DO  - 10.1111/fcp.12415
SP  - 199
EP  - 207
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2019",
abstract = "Dexamethasone (Dx) is often used in obstetric practice to promote fetal lung maturation and to prevent respiratory distress syndrome when the risk of preterm delivery persists. This therapy enables survival of the newborn, but also is associated with deleterious effects on the offspring, such as reproductive disorders. The aim of this study was to determine specifically whether prenatal exposure to Dx disturbs the physiological balance between proliferation and apoptosis of germinative cells (GC) in the ovary of 19 and 21 day old fetuses and thus induces developmental programing of the female reproductive system. Pregnant Wistar rats (n = 10/group), separated into control (vehicle) and Dx-treated (0.5 mg/kg body mass) groups, received injections on gestational days 16, 17 and 18. Exposure to Dx lowered the volume of the fetal ovary by 30% (p<0.05) in 21 day old fetuses, as well as the total number of GC in the ovary by 21% (p<0.05). When compared to the controls, in Dx-exposed fetuses the total number of PCNA positive GC was 27% lower at 19 days and 71% lower at 21 day old (p<0.05), while total numbers of caspase-3 positive GC were 2.3 fold and 34% higher respectively, (p<0.05). Our results demonstrate that prenatal exposure to Dx diminished proliferation but increased the rate of germinative cell apoptosis, with consequently reduced total germinative cell number and ovary volume. Impairment of fetal oogenesis and fewer GC in the fetal ovary compromise the oogonial stock and thus may constitute a risk for female fertility. This article is protected by copyright. All rights reserved.",
journal = "Fundamental & Clinical Pharmacology",
title = "Adverse effect of dexamethasone on development of the fetal rat ovary.",
number = "2",
volume = "33",
doi = "10.1111/fcp.12415",
pages = "199-207"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2019). Adverse effect of dexamethasone on development of the fetal rat ovary.. in Fundamental & Clinical Pharmacology, 33(2), 199-207.
https://doi.org/10.1111/fcp.12415

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Adverse effect of dexamethasone on development of the fetal rat ovary.. in Fundamental & Clinical Pharmacology. 2019;33(2):199-207.
doi:10.1111/fcp.12415 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Adverse effect of dexamethasone on development of the fetal rat ovary." in Fundamental & Clinical Pharmacology, 33, no. 2 (2019):199-207,
https://doi.org/10.1111/fcp.12415 . .

TY  - JOUR
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0065128117303616
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29338944
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2962
AB  - The isoflavone, daidzein is a biologically active, plant-derived compound that interacts with estrogen receptors. Data from previous studies have suggested that daidzein exerts beneficial effects in many diseases; however, as an endocrine disrupter, it may also alter the functioning of the endocrine system. Data regarding the effect of daidzein on the morphofunctional and histological parameters of the hypothalamic-pituitary-adrenal (HPA) system is still lacking. Therefore, using the newCAST stereological software, we investigated the effects of chronic (21 days) daidzein treatment on corticotropin-releasing hormone (CRH) neurons within the hypothalamus and corticotropes (ACTH cells) in the pituitary, while image analysis was employed to-examine the intensity of fluorescence of CRH in the median eminence (ME) and adrenocorticotropin hormone in the pituitary in adult orchidectomized (Ovx) rats. Circulating ACTH and corticosterone levels were also analyzed. This study showed that daidzein treatment decreased the volume density of CRH neurons within the paraventricular nucleus as well as CRH immunofluorescence in the ME. The total number of ACTH cells was decreased, while ACTH cell volume and the intensity of ACTH fluorescence were increased following daidzein treatment. Both ACTH and corticosterone blood levels were increased after daidzein administration. The results of performed experiments clearly demonstrate that volume density of CRH neurons; total number and volume of ACTH cells, as well as stress hormones levels are vulnerable to the effects of daidzein.
T2  - Acta Histochemica
T1  - Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat.
IS  - 2
VL  - 120
DO  - 10.1016/j.acthis.2017.12.006
SP  - 129
EP  - 135
ER  - 

@article{
author = "Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Ristić, Nataša and Šošić-Jurjević, Branka  and Pendovski, Lazo and Milošević, Verica",
year = "2018",
abstract = "The isoflavone, daidzein is a biologically active, plant-derived compound that interacts with estrogen receptors. Data from previous studies have suggested that daidzein exerts beneficial effects in many diseases; however, as an endocrine disrupter, it may also alter the functioning of the endocrine system. Data regarding the effect of daidzein on the morphofunctional and histological parameters of the hypothalamic-pituitary-adrenal (HPA) system is still lacking. Therefore, using the newCAST stereological software, we investigated the effects of chronic (21 days) daidzein treatment on corticotropin-releasing hormone (CRH) neurons within the hypothalamus and corticotropes (ACTH cells) in the pituitary, while image analysis was employed to-examine the intensity of fluorescence of CRH in the median eminence (ME) and adrenocorticotropin hormone in the pituitary in adult orchidectomized (Ovx) rats. Circulating ACTH and corticosterone levels were also analyzed. This study showed that daidzein treatment decreased the volume density of CRH neurons within the paraventricular nucleus as well as CRH immunofluorescence in the ME. The total number of ACTH cells was decreased, while ACTH cell volume and the intensity of ACTH fluorescence were increased following daidzein treatment. Both ACTH and corticosterone blood levels were increased after daidzein administration. The results of performed experiments clearly demonstrate that volume density of CRH neurons; total number and volume of ACTH cells, as well as stress hormones levels are vulnerable to the effects of daidzein.",
journal = "Acta Histochemica",
title = "Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat.",
number = "2",
volume = "120",
doi = "10.1016/j.acthis.2017.12.006",
pages = "129-135"
}

Trifunović, S., Manojlović-Stojanoski, M., Nestorović, N., Ristić, N., Šošić-Jurjević, B., Pendovski, L.,& Milošević, V.. (2018). Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat.. in Acta Histochemica, 120(2), 129-135.
https://doi.org/10.1016/j.acthis.2017.12.006

Trifunović S, Manojlović-Stojanoski M, Nestorović N, Ristić N, Šošić-Jurjević B, Pendovski L, Milošević V. Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat.. in Acta Histochemica. 2018;120(2):129-135.
doi:10.1016/j.acthis.2017.12.006 .

Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Nestorović, Nataša, Ristić, Nataša, Šošić-Jurjević, Branka , Pendovski, Lazo, Milošević, Verica, "Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat." in Acta Histochemica, 120, no. 2 (2018):129-135,
https://doi.org/10.1016/j.acthis.2017.12.006 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Miljić, Dragana
AU  - Ristić, Nataša
AU  - Pendovski, Lazo
AU  - Popovska-Perčinić, Florina
AU  - Rovčanin, Zoran
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6240
AB  - Herein, we have tested the effects of application of commercial soy extract, rich in estrogen-like isoflavones, to orchidectomized adult rats (the model of mild andropause), on the corticosteroid secretion. Exploited animal model provides the opportunity to examine the potential effects of steroid-like compounds in the hormonal milieu deprived of endogenous sex steroids along with preserved hippocampal and hypothalamic regulation of the adrenal gland function. The experimental groups included sham-operated (SO), orchidectomized (Orx) and soy extract-treated orchidectomized (Orx + Soy; 30 mg/kg b.m. s.c., for 3 weeks) rats. Plasma level of ACTH and serum levels of aldosterone, corticosterone and DHEA were determined by the immunoassays. Orchidectomy caused the increase (P<0.05) of circulating ACTH, aldosterone and DHEA by 57.0%. 2.6 and 2.0 folds respectively, compared to SO group. In Orx + Soy group, blood levels of ACTH and corticosterone were higher (P<0.05) by 99.5% and 79.6% respectively, in comparison with SO group. Also, the treatment of orchidectomized rats with soy extract elevated (P<0.05) the systemic levels of ACTH, aldosterone and corticosterone by 26.9 %, 2.7 folds and 56.4% respectively, while the circulating DHEA level was decreased (65.6%; P<0.05), all compared to the corresponding parameters in Orx rats. Summarily, soy extract raised the ACTH level which has stimulatory influence on the mineralocorticoid and glucocorticoid output, while the adrenal androgens consequently fallen, in the rat model of mild andropause. The data support impression of a beneficial effect of soy isoflavone mixture on the homeostatic response to stress, but also call for precautions in the case of cardiovascular issue presence.
PB  - bioscientifica
C3  - 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
T1  - Corticosteroid secretion after the soy extract application to orchidectomized adult male rats
DO  - 10.1530/endoabs.56.P621
SP  - 382
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Miljić, Dragana and Ristić, Nataša and Pendovski, Lazo and Popovska-Perčinić, Florina and Rovčanin, Zoran and Milošević, Verica",
year = "2018",
abstract = "Herein, we have tested the effects of application of commercial soy extract, rich in estrogen-like isoflavones, to orchidectomized adult rats (the model of mild andropause), on the corticosteroid secretion. Exploited animal model provides the opportunity to examine the potential effects of steroid-like compounds in the hormonal milieu deprived of endogenous sex steroids along with preserved hippocampal and hypothalamic regulation of the adrenal gland function. The experimental groups included sham-operated (SO), orchidectomized (Orx) and soy extract-treated orchidectomized (Orx + Soy; 30 mg/kg b.m. s.c., for 3 weeks) rats. Plasma level of ACTH and serum levels of aldosterone, corticosterone and DHEA were determined by the immunoassays. Orchidectomy caused the increase (P<0.05) of circulating ACTH, aldosterone and DHEA by 57.0%. 2.6 and 2.0 folds respectively, compared to SO group. In Orx + Soy group, blood levels of ACTH and corticosterone were higher (P<0.05) by 99.5% and 79.6% respectively, in comparison with SO group. Also, the treatment of orchidectomized rats with soy extract elevated (P<0.05) the systemic levels of ACTH, aldosterone and corticosterone by 26.9 %, 2.7 folds and 56.4% respectively, while the circulating DHEA level was decreased (65.6%; P<0.05), all compared to the corresponding parameters in Orx rats. Summarily, soy extract raised the ACTH level which has stimulatory influence on the mineralocorticoid and glucocorticoid output, while the adrenal androgens consequently fallen, in the rat model of mild andropause. The data support impression of a beneficial effect of soy isoflavone mixture on the homeostatic response to stress, but also call for precautions in the case of cardiovascular issue presence.",
publisher = "bioscientifica",
journal = "20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain",
title = "Corticosteroid secretion after the soy extract application to orchidectomized adult male rats",
doi = "10.1530/endoabs.56.P621",
pages = "382"
}

Ajdžanović, V., Miljić, D., Ristić, N., Pendovski, L., Popovska-Perčinić, F., Rovčanin, Z.,& Milošević, V.. (2018). Corticosteroid secretion after the soy extract application to orchidectomized adult male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain
bioscientifica., 382.
https://doi.org/10.1530/endoabs.56.P621

Ajdžanović V, Miljić D, Ristić N, Pendovski L, Popovska-Perčinić F, Rovčanin Z, Milošević V. Corticosteroid secretion after the soy extract application to orchidectomized adult male rats. in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain. 2018;:382.
doi:10.1530/endoabs.56.P621 .

Ajdžanović, Vladimir, Miljić, Dragana, Ristić, Nataša, Pendovski, Lazo, Popovska-Perčinić, Florina, Rovčanin, Zoran, Milošević, Verica, "Corticosteroid secretion after the soy extract application to orchidectomized adult male rats" in 20th European Congress of Endocrinology; 2018 May 19-22; Barcelona, Spain (2018):382,
https://doi.org/10.1530/endoabs.56.P621 . .

TY  - CONF
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
AU  - Pendovski, Lazo
AU  - Potrebić, Milica
AU  - Ušćebrka, Gordana
AU  - Sretenović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.enea2018.com/program.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5925
PB  - European NeuroEndocrine Association
C3  - 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
T1  - The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5925
ER  - 

@conference{
author = "Popovska-Perčinić, Florina and Milošević, Verica and Pendovski, Lazo and Potrebić, Milica and Ušćebrka, Gordana and Sretenović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir",
year = "2018",
publisher = "European NeuroEndocrine Association",
journal = "18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland",
title = "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5925"
}

Popovska-Perčinić, F., Milošević, V., Pendovski, L., Potrebić, M., Ušćebrka, G., Sretenović, J., Jarić, I.,& Ajdžanović, V.. (2018). The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
European NeuroEndocrine Association., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925

Popovska-Perčinić F, Milošević V, Pendovski L, Potrebić M, Ušćebrka G, Sretenović J, Jarić I, Ajdžanović V. The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland. 2018;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

Popovska-Perčinić, Florina, Milošević, Verica, Pendovski, Lazo, Potrebić, Milica, Ušćebrka, Gordana, Sretenović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study" in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland (2018):8,
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2018
UR  - http://doi.wiley.com/10.1111/fcp.12415
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3155
AB  - Dexamethasone (Dx) is often used in obstetric practice to promote fetal lung maturation and to prevent respiratory distress syndrome when the risk of preterm delivery persists. This therapy enables survival of the newborn, but also is associated with deleterious effects on the offspring, such as reproductive disorders. The aim of this study was to determine specifically whether prenatal exposure to Dx disturbs the physiological balance between proliferation and apoptosis of germinative cells (GC) in the ovary of 19 and 21 day old fetuses and thus induces developmental programing of the female reproductive system. Pregnant Wistar rats (n = 10/group), separated into control (vehicle) and Dx-treated (0.5 mg/kg body mass) groups, received injections on gestational days 16, 17 and 18. Exposure to Dx lowered the volume of the fetal ovary by 30% (p<0.05) in 21 day old fetuses, as well as the total number of GC in the ovary by 21% (p<0.05). When compared to the controls, in Dx-exposed fetuses the total number of PCNA positive GC was 27% lower at 19 days and 71% lower at 21 day old (p<0.05), while total numbers of caspase-3 positive GC were 2.3 fold and 34% higher respectively, (p<0.05). Our results demonstrate that prenatal exposure to Dx diminished proliferation but increased the rate of germinative cell apoptosis, with consequently reduced total germinative cell number and ovary volume. Impairment of fetal oogenesis and fewer GC in the fetal ovary compromise the oogonial stock and thus may constitute a risk for female fertility. This article is protected by copyright. All rights reserved.
T2  - Fundamental & clinical pharmacology
T1  - Adverse effect of dexamethasone on development of the fetal rat ovary.
DO  - 10.1111/fcp.12415
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2018",
abstract = "Dexamethasone (Dx) is often used in obstetric practice to promote fetal lung maturation and to prevent respiratory distress syndrome when the risk of preterm delivery persists. This therapy enables survival of the newborn, but also is associated with deleterious effects on the offspring, such as reproductive disorders. The aim of this study was to determine specifically whether prenatal exposure to Dx disturbs the physiological balance between proliferation and apoptosis of germinative cells (GC) in the ovary of 19 and 21 day old fetuses and thus induces developmental programing of the female reproductive system. Pregnant Wistar rats (n = 10/group), separated into control (vehicle) and Dx-treated (0.5 mg/kg body mass) groups, received injections on gestational days 16, 17 and 18. Exposure to Dx lowered the volume of the fetal ovary by 30% (p<0.05) in 21 day old fetuses, as well as the total number of GC in the ovary by 21% (p<0.05). When compared to the controls, in Dx-exposed fetuses the total number of PCNA positive GC was 27% lower at 19 days and 71% lower at 21 day old (p<0.05), while total numbers of caspase-3 positive GC were 2.3 fold and 34% higher respectively, (p<0.05). Our results demonstrate that prenatal exposure to Dx diminished proliferation but increased the rate of germinative cell apoptosis, with consequently reduced total germinative cell number and ovary volume. Impairment of fetal oogenesis and fewer GC in the fetal ovary compromise the oogonial stock and thus may constitute a risk for female fertility. This article is protected by copyright. All rights reserved.",
journal = "Fundamental & clinical pharmacology",
title = "Adverse effect of dexamethasone on development of the fetal rat ovary.",
doi = "10.1111/fcp.12415"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2018). Adverse effect of dexamethasone on development of the fetal rat ovary.. in Fundamental & clinical pharmacology.
https://doi.org/10.1111/fcp.12415

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Adverse effect of dexamethasone on development of the fetal rat ovary.. in Fundamental & clinical pharmacology. 2018;.
doi:10.1111/fcp.12415 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Adverse effect of dexamethasone on development of the fetal rat ovary." in Fundamental & clinical pharmacology (2018),
https://doi.org/10.1111/fcp.12415 . .

TY  - JOUR
AU  - Popovska-Perčinić, Florina
AU  - Jarić, Ivana
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
PY  - 2017
UR  - http://www.degruyter.com/view/j/acve.2017.67.issue-4/acve-2017-0040/acve-2017-0040.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2994
AB  - In areas with moderate continental climate, increased average ambient temperature during the summer represents a stressogenic factor that affects the hypothalamo-pituitaryadrenocortical axis in mammals. Therefore, we wanted to examine the effects of 4 days of constant exposure to moderately elevated ambient temperature (35 ± 1oC) on the histomorphometric and immunofl uorescent characteristics, as well as on the hormonal secretion of pituitary corticotropes (ACTH) cells in adult male rats. In comparison with the controls kept at 20 ± 2oC, a signifi cant increase (p<0.05) of the absolute and relative pituitary weight (23.1% and 36.1%, respectively) was registered after exposure to heat. The localization, as well as the shape of the ACTH cells in the heat exposed group was not signifi cantly altered, but their immunopositivity was weaker. After 4 days of heat exposure, a weaker signal confi rmed the relative fl uorescence intensity of the ACTH cells (15.3%, p<0.05). In heat exposed rats, an increase of the cellular and nuclear volumes of immunolabelled ACTH cells and decrease of their volume density (6.9%, 14.3% and 20.0%, respectively; p<0.05) was registered. Observed histomorphometric and immunofl uorescent features of the pituitary ACTH cells were in accordance with the increased (p<0.05) value of plasma adrenocorticotropic hormone (ACTH) by 23.7% compared to the control rats. It can be concluded that the 4-day exposure to moderately elevated ambient temperature intensifi es pituitary ACTH secretion in adult male rats.
T2  - Acta Veterinaria
T1  - The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study
IS  - 4
VL  - 67
DO  - 10.1515/acve-2017-0040
SP  - 495
EP  - 507
ER  - 

@article{
author = "Popovska-Perčinić, Florina and Jarić, Ivana and Pendovski, Lazo and Ristić, Nataša and Trifunović, Svetlana and Milošević, Verica and Ajdžanović, Vladimir",
year = "2017",
abstract = "In areas with moderate continental climate, increased average ambient temperature during the summer represents a stressogenic factor that affects the hypothalamo-pituitaryadrenocortical axis in mammals. Therefore, we wanted to examine the effects of 4 days of constant exposure to moderately elevated ambient temperature (35 ± 1oC) on the histomorphometric and immunofl uorescent characteristics, as well as on the hormonal secretion of pituitary corticotropes (ACTH) cells in adult male rats. In comparison with the controls kept at 20 ± 2oC, a signifi cant increase (p<0.05) of the absolute and relative pituitary weight (23.1% and 36.1%, respectively) was registered after exposure to heat. The localization, as well as the shape of the ACTH cells in the heat exposed group was not signifi cantly altered, but their immunopositivity was weaker. After 4 days of heat exposure, a weaker signal confi rmed the relative fl uorescence intensity of the ACTH cells (15.3%, p<0.05). In heat exposed rats, an increase of the cellular and nuclear volumes of immunolabelled ACTH cells and decrease of their volume density (6.9%, 14.3% and 20.0%, respectively; p<0.05) was registered. Observed histomorphometric and immunofl uorescent features of the pituitary ACTH cells were in accordance with the increased (p<0.05) value of plasma adrenocorticotropic hormone (ACTH) by 23.7% compared to the control rats. It can be concluded that the 4-day exposure to moderately elevated ambient temperature intensifi es pituitary ACTH secretion in adult male rats.",
journal = "Acta Veterinaria",
title = "The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study",
number = "4",
volume = "67",
doi = "10.1515/acve-2017-0040",
pages = "495-507"
}

Popovska-Perčinić, F., Jarić, I., Pendovski, L., Ristić, N., Trifunović, S., Milošević, V.,& Ajdžanović, V.. (2017). The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study. in Acta Veterinaria, 67(4), 495-507.
https://doi.org/10.1515/acve-2017-0040

Popovska-Perčinić F, Jarić I, Pendovski L, Ristić N, Trifunović S, Milošević V, Ajdžanović V. The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study. in Acta Veterinaria. 2017;67(4):495-507.
doi:10.1515/acve-2017-0040 .

Popovska-Perčinić, Florina, Jarić, Ivana, Pendovski, Lazo, Ristić, Nataša, Trifunović, Svetlana, Milošević, Verica, Ajdžanović, Vladimir, "The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study" in Acta Veterinaria, 67, no. 4 (2017):495-507,
https://doi.org/10.1515/acve-2017-0040 . .

TY  - CONF
AU  - Ristić, Nataša
AU  - Pendovski, Lazo
AU  - Manojlović-Stojanoski, Milica
AU  - Miljić, Dragana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Nestorović, Nataša
AU  - Milošević, Verica
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3431
AB  - Glucocorticoids affect the growth and maturation of fetal organ systems, but overexposure to exogenous glucocorticoids retard fetal growth and may alter developmental process in sensitive tissues. On the other hand, fetal ovary is not characterized by definitive follicular structure, but the clusters of germinative cells are predominant. Germ cells may be in meiotic prophase or with sign of degeneration.

The aim of this study was to determine whether prenatal exposure to dexamethasone (Dx) altered normal structure and development of ovary in 19 day old rat fetuses. Pregnant females in the experimental group received subcutaneous injections of 1.0, 0.5 and 0.5 mg Dx /kg body weight on days 16–18 of pregnancy. Control mothers were injected with the same amount of saline. On day 19 of pregnancy, the dams and their fetuses were sacrificed under ether anesthesia and the fetuses were referred to as 19-day-old fetuses. Fetal ovaries were prepared for further stereological examination.

The volume of the fetal ovary estimated using Cavalieri’s principle was significantly reduced after exposure to Dx by 22% (P<0.05) in comparison with control ovary. Using a fractionator-physical disector method, a reduction in total number of germ cells in meiotic prophase by 43% (P<0.05) was observed, while total number of germ cells with sign of degeneration increased by 54% (P<0.05), when compared with control values.

Overexposure to dexamethasone during fetal development intensifies the process of degeneration of germ cells in the ovary, contributing thus the reduction of reproductive potential for the individual.
PB  - BioScientifica
C3  - 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)
T1  - Effects of prenatal dexamethasone on fetal rat ovary
DO  - 10.1530/endoabs.49.GP62
SP  - GP62
ER  - 

@conference{
author = "Ristić, Nataša and Pendovski, Lazo and Manojlović-Stojanoski, Milica and Miljić, Dragana and Miler, Marko and Ajdžanović, Vladimir and Trifunović, Svetlana and Nestorović, Nataša and Milošević, Verica",
year = "2017",
abstract = "Glucocorticoids affect the growth and maturation of fetal organ systems, but overexposure to exogenous glucocorticoids retard fetal growth and may alter developmental process in sensitive tissues. On the other hand, fetal ovary is not characterized by definitive follicular structure, but the clusters of germinative cells are predominant. Germ cells may be in meiotic prophase or with sign of degeneration.

The aim of this study was to determine whether prenatal exposure to dexamethasone (Dx) altered normal structure and development of ovary in 19 day old rat fetuses. Pregnant females in the experimental group received subcutaneous injections of 1.0, 0.5 and 0.5 mg Dx /kg body weight on days 16–18 of pregnancy. Control mothers were injected with the same amount of saline. On day 19 of pregnancy, the dams and their fetuses were sacrificed under ether anesthesia and the fetuses were referred to as 19-day-old fetuses. Fetal ovaries were prepared for further stereological examination.

The volume of the fetal ovary estimated using Cavalieri’s principle was significantly reduced after exposure to Dx by 22% (P<0.05) in comparison with control ovary. Using a fractionator-physical disector method, a reduction in total number of germ cells in meiotic prophase by 43% (P<0.05) was observed, while total number of germ cells with sign of degeneration increased by 54% (P<0.05), when compared with control values.

Overexposure to dexamethasone during fetal development intensifies the process of degeneration of germ cells in the ovary, contributing thus the reduction of reproductive potential for the individual.",
publisher = "BioScientifica",
journal = "19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)",
title = "Effects of prenatal dexamethasone on fetal rat ovary",
doi = "10.1530/endoabs.49.GP62",
pages = "GP62"
}

Ristić, N., Pendovski, L., Manojlović-Stojanoski, M., Miljić, D., Miler, M., Ajdžanović, V., Trifunović, S., Nestorović, N.,& Milošević, V.. (2017). Effects of prenatal dexamethasone on fetal rat ovary. in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)
BioScientifica., GP62.
https://doi.org/10.1530/endoabs.49.GP62

Ristić N, Pendovski L, Manojlović-Stojanoski M, Miljić D, Miler M, Ajdžanović V, Trifunović S, Nestorović N, Milošević V. Effects of prenatal dexamethasone on fetal rat ovary. in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49). 2017;:GP62.
doi:10.1530/endoabs.49.GP62 .

Ristić, Nataša, Pendovski, Lazo, Manojlović-Stojanoski, Milica, Miljić, Dragana, Miler, Marko, Ajdžanović, Vladimir, Trifunović, Svetlana, Nestorović, Nataša, Milošević, Verica, "Effects of prenatal dexamethasone on fetal rat ovary" in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49) (2017):GP62,
https://doi.org/10.1530/endoabs.49.GP62 . .

TY  - CONF
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Živanović, Jasmina
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6281
AB  - Introduction: The soybean isoflavones with estrogenic activity are used as natural substitute for hormone replacement therapy for prevention and treatment of postmenopausal osteoporosis. In recent years, more attention has focused on role of estrogens on bone metabolism in men. Therefore, the aim of this study was to investigate the effect of isoflavone genistein on the trabecular bone in orchidectomized (Orx) middle-aged rats as animal model of male osteoporosis.
Material and Methods: Sixteen-month-old Wistar rats were divided into Orx, and a sham-operated (SO) group. Two weeks after gonadectomy, subcutaneous injections of genistein (30 mg/kg b.w./day) were administered for 3 weeks. The SO and one Orx group received sterile olive oil. The animals were killed 24 h after last injection. After fixation and decalcification the proximal tibias were stained by an Azan method. An lmageJ public domain image processing program was used for histomorphometric measurements in the proximal tibial metaphysis and calculate cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp). Serum samples were analyzed for osteocalcin (OC) levels, and urine samples for calcium (Ca2+) levels. The data were assessed for normal distribution by the Kolmogorov­ Smirnov test, followed by one-way analysis of variance (ANOVA).
Results: Analysis of trabecular microarchitecture of proximal tibia in the Orx rats showed a 
significant decreases of B.Ar, Tb.Th and Tb.N whereas Tb.Sp was significantly increased compared to SO. After Orx, serum OC and urinary Ca2+ concentration significantly increased in comparison with SO. Genistein treatment in Orx rats significantly enhanced B.Ar, Tb.Th and Tb.N by 115%, 26% and 34% (p<0.05) respectively; but Tb.Sp was 38% lower (p<0.05) when compared to the Orx. Serum OC was 31% lower (p<0.05) after genistein administration compared to values for the Orx rats. Urinary Ca2+ concentration was lower than in the Orx by 68% (p<0.05).
Conclusion: These results suggest that chronic genistein treatment increase trabecular bone mass and decrease bone turnover in the rat model of male osteoporosis.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedoina
T1  - Genistein stimulates trabecular bone structure in orchidectomized middle aged male rats
SP  - 128
EP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6281
ER  - 

@conference{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanović, Vladimir and Ristić, Nataša and Trifunović, Svetlana and Živanović, Jasmina and Pendovski, Lazo and Milošević, Verica",
year = "2016",
abstract = "Introduction: The soybean isoflavones with estrogenic activity are used as natural substitute for hormone replacement therapy for prevention and treatment of postmenopausal osteoporosis. In recent years, more attention has focused on role of estrogens on bone metabolism in men. Therefore, the aim of this study was to investigate the effect of isoflavone genistein on the trabecular bone in orchidectomized (Orx) middle-aged rats as animal model of male osteoporosis.
Material and Methods: Sixteen-month-old Wistar rats were divided into Orx, and a sham-operated (SO) group. Two weeks after gonadectomy, subcutaneous injections of genistein (30 mg/kg b.w./day) were administered for 3 weeks. The SO and one Orx group received sterile olive oil. The animals were killed 24 h after last injection. After fixation and decalcification the proximal tibias were stained by an Azan method. An lmageJ public domain image processing program was used for histomorphometric measurements in the proximal tibial metaphysis and calculate cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp). Serum samples were analyzed for osteocalcin (OC) levels, and urine samples for calcium (Ca2+) levels. The data were assessed for normal distribution by the Kolmogorov­ Smirnov test, followed by one-way analysis of variance (ANOVA).
Results: Analysis of trabecular microarchitecture of proximal tibia in the Orx rats showed a 
significant decreases of B.Ar, Tb.Th and Tb.N whereas Tb.Sp was significantly increased compared to SO. After Orx, serum OC and urinary Ca2+ concentration significantly increased in comparison with SO. Genistein treatment in Orx rats significantly enhanced B.Ar, Tb.Th and Tb.N by 115%, 26% and 34% (p<0.05) respectively; but Tb.Sp was 38% lower (p<0.05) when compared to the Orx. Serum OC was 31% lower (p<0.05) after genistein administration compared to values for the Orx rats. Urinary Ca2+ concentration was lower than in the Orx by 68% (p<0.05).
Conclusion: These results suggest that chronic genistein treatment increase trabecular bone mass and decrease bone turnover in the rat model of male osteoporosis.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedoina",
title = "Genistein stimulates trabecular bone structure in orchidectomized middle aged male rats",
pages = "128-129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6281"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Ristić, N., Trifunović, S., Živanović, J., Pendovski, L.,& Milošević, V.. (2016). Genistein stimulates trabecular bone structure in orchidectomized middle aged male rats. in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedoina
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 128-129.
https://hdl.handle.net/21.15107/rcub_ibiss_6281

Filipović B, Šošić-Jurjević B, Ajdžanović V, Ristić N, Trifunović S, Živanović J, Pendovski L, Milošević V. Genistein stimulates trabecular bone structure in orchidectomized middle aged male rats. in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedoina. 2016;:128-129.
https://hdl.handle.net/21.15107/rcub_ibiss_6281 .

Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanović, Vladimir, Ristić, Nataša, Trifunović, Svetlana, Živanović, Jasmina, Pendovski, Lazo, Milošević, Verica, "Genistein stimulates trabecular bone structure in orchidectomized middle aged male rats" in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedoina (2016):128-129,
https://hdl.handle.net/21.15107/rcub_ibiss_6281 .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Severs, Walter
AU  - Nestorović, Nataša
AU  - Jarić, Ivana
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2024
AB  - Glucocorticoids have a strong influence on growth and maturation of
   fetal organ systems, but overexposure to exogenous glucocorticoids may
   retard fetal growth and alter developmental processes in sensitive
   tissues. The aim of this study was to specifically determine whether
   prenatal exposure to dexamethasone (Dx) altered normal development and
   function of pituitary gonadotropic cells in neonatal, infant and
   peripubertal female offspring. On day 16 of pregnancy, rat dams received
   1.0 mg Dx/kg body weight (BW) s.c., followed by 0.5 mg Dx/kg BW on days
   17 and 18 of gestation. Control gravid females received the same volume
   of saline. Female offspring were sacrificed on days 5, 16 and 38 after
   delivery. The volume of the pituitary gland estimated using Cavalieri's
   principle was significantly reduced (p < 0.05). Using a
   fractionator-physical disector method, we found reduced total numbers of
   follicle-stimulating hormone (FSH) and luteinizing hormone (LH) cells (p
   < 0.05), accompanied by a decrease (p < 0.05) in serum concentrations of
   FSH and LH, while the relative intensity of FSH and LH
   immunofluorescence remained unchanged in neonatal, infant and
   peripubertal female offspring prenatally exposed to Dx. The data
   document that overexposure to Dx during fetal development evokes
   developmental programming of the female reproductive system at the
   pituitary cellular level, which may be associated with impaired
   reproductive function. (C) 2016 S. Karger AG, Basel
T2  - Cells Tissues Organs
T1  - Effects of Prenatal Dexamethasone on the Rat Pituitary Gland and
 Gonadotropic Cells in Female Offspring
IS  - 2
VL  - 201
DO  - 10.1159/000443987
SP  - 148
EP  - 158
ER  - 

@article{
author = "Ristić, Nataša and Severs, Walter and Nestorović, Nataša and Jarić, Ivana and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Pendovski, Lazo and Milošević, Verica",
year = "2015",
abstract = "Glucocorticoids have a strong influence on growth and maturation of
   fetal organ systems, but overexposure to exogenous glucocorticoids may
   retard fetal growth and alter developmental processes in sensitive
   tissues. The aim of this study was to specifically determine whether
   prenatal exposure to dexamethasone (Dx) altered normal development and
   function of pituitary gonadotropic cells in neonatal, infant and
   peripubertal female offspring. On day 16 of pregnancy, rat dams received
   1.0 mg Dx/kg body weight (BW) s.c., followed by 0.5 mg Dx/kg BW on days
   17 and 18 of gestation. Control gravid females received the same volume
   of saline. Female offspring were sacrificed on days 5, 16 and 38 after
   delivery. The volume of the pituitary gland estimated using Cavalieri's
   principle was significantly reduced (p < 0.05). Using a
   fractionator-physical disector method, we found reduced total numbers of
   follicle-stimulating hormone (FSH) and luteinizing hormone (LH) cells (p
   < 0.05), accompanied by a decrease (p < 0.05) in serum concentrations of
   FSH and LH, while the relative intensity of FSH and LH
   immunofluorescence remained unchanged in neonatal, infant and
   peripubertal female offspring prenatally exposed to Dx. The data
   document that overexposure to Dx during fetal development evokes
   developmental programming of the female reproductive system at the
   pituitary cellular level, which may be associated with impaired
   reproductive function. (C) 2016 S. Karger AG, Basel",
journal = "Cells Tissues Organs",
title = "Effects of Prenatal Dexamethasone on the Rat Pituitary Gland and
 Gonadotropic Cells in Female Offspring",
number = "2",
volume = "201",
doi = "10.1159/000443987",
pages = "148-158"
}

Ristić, N., Severs, W., Nestorović, N., Jarić, I., Manojlović-Stojanoski, M., Trifunović, S., Pendovski, L.,& Milošević, V.. (2015). Effects of Prenatal Dexamethasone on the Rat Pituitary Gland and
 Gonadotropic Cells in Female Offspring. in Cells Tissues Organs, 201(2), 148-158.
https://doi.org/10.1159/000443987

Ristić N, Severs W, Nestorović N, Jarić I, Manojlović-Stojanoski M, Trifunović S, Pendovski L, Milošević V. Effects of Prenatal Dexamethasone on the Rat Pituitary Gland and
 Gonadotropic Cells in Female Offspring. in Cells Tissues Organs. 2015;201(2):148-158.
doi:10.1159/000443987 .

Ristić, Nataša, Severs, Walter, Nestorović, Nataša, Jarić, Ivana, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Pendovski, Lazo, Milošević, Verica, "Effects of Prenatal Dexamethasone on the Rat Pituitary Gland and
 Gonadotropic Cells in Female Offspring" in Cells Tissues Organs, 201, no. 2 (2015):148-158,
https://doi.org/10.1159/000443987 . .