@article{
author = "Lavrnja, Irena and Parabucki, Ana and Brkić, Predrag and Jovanović, Tomislav and Dacic, Sanja and Savić, Danijela and Pantić, Igor and Stojiljković, Mirjana and Peković, Sanja",
year = "2015",
abstract = "The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
exerts its beneficial effects on recovery after brain injury are still
unrevealed. Therefore, in this study we investigated the influence of
repetitive HBOT on the reactive astrogliosis and expression of mediators
of inflammation after cortical stab injury (CSI). CSI was performed on
male Wistar rats, divided into control, sham, and lesioned groups with
appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
minutes of slow decompression, once a day for 10 consecutive days. Data
obtained using real-time polymerase chain reaction, Western blot, and
immunohistochemical and immunofluorescence analyses revealed that
repetitive HBOT applied after the CSI attenuates reactive astrogliosis
and glial scarring, and reduces expression of GFAP (glial fibrillary
acidic protein), vimentin, and ICAM-1 (intercellular adhesion
molecule-1) both at gene and tissue levels. In addition, HBOT prevents
expression of CD40 and its ligand CD40L on microglia, neutrophils,
cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
by prevention of glial scarring and limiting of expression of
inflammatory mediators, supports formation of more permissive
environment for repair and regeneration.",
journal = "Mediators of Inflammation",
title = "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
Suppresses Expression of Inflammatory Mediators in the Rat Model of
Brain Injury",
number = "498405",
doi = "10.1155/2015/498405"
}
