TY  - JOUR
AU  - Garcia-Oliveira, Paula
AU  - Carreira-Casais, Anxo
AU  - Pereira, Eliana
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Prieto, Miguel A
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9571014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5170
AB  - Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.
PB  - Basel: MDPI
T2  - Molecules
T1  - From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants
IS  - 19
VL  - 27
DO  - 10.3390/molecules27196495
ER  - 

@article{
author = "Garcia-Oliveira, Paula and Carreira-Casais, Anxo and Pereira, Eliana and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C and Stojković, Dejan and Soković, Marina and Simal-Gandara, Jesus and Prieto, Miguel A and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants",
number = "19",
volume = "27",
doi = "10.3390/molecules27196495"
}

Garcia-Oliveira, P., Carreira-Casais, A., Pereira, E., Dias, M. I., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Simal-Gandara, J., Prieto, M. A., Caleja, C.,& Barros, L.. (2022). From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules
Basel: MDPI., 27(19).
https://doi.org/10.3390/molecules27196495

Garcia-Oliveira P, Carreira-Casais A, Pereira E, Dias MI, Pereira C, Calhelha RC, Stojković D, Soković M, Simal-Gandara J, Prieto MA, Caleja C, Barros L. From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules. 2022;27(19).
doi:10.3390/molecules27196495 .

Garcia-Oliveira, Paula, Carreira-Casais, Anxo, Pereira, Eliana, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C, Stojković, Dejan, Soković, Marina, Simal-Gandara, Jesus, Prieto, Miguel A, Caleja, Cristina, Barros, Lillian, "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196495 . .

TY  - CONF
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Petrović, Jovana
AU  - Prieto, Miguel Angel
AU  - Soković, Marina
AU  - Ferreira, Isabel
AU  - Simal-Gandara, Jesus
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6691
AB  - European consumers have a growing interest in new fruits and flavours, which has promoted the production and commercialisation of exotic fruits such as goldenberry (Physalis peruviana L., Fam. Solanaceae). Colombia and South Africa are currently the main producers of this fruit, but it can be cultivated in almost all the highlands of the tropics and in several parts of the subtropics, given its ability to adapt to a wide range of agroecological conditions [1]. Consumer demand for this small orange berry has also been driven by its nutritional value and health-promoting effects [2]. Although there have been different studies about this fruit, the available information is still scarce. Furthermore, the fruit quality attributes can vary depending on the agricultural practices used and the edaphoclimatic conditions of the growing sites. Therefore, this study was carried out to evaluate the nutritional composition and in vitro antioxidant and antimicrobial activity of goldenberry cultivated in the northeast region of Portugal. Goldenberry samples were lyophilised and analysed to determine their proximate composition (moisture, protein, fat, ash, dietary fibre, and carbohydrates), following official methods of food analysis [3]. Their profiles of sugars, organic acids, fatty acids, and tocopherols were characterised by standardised chromatographic methods [3]. The powdered goldenberry sample was also prepared in a hydroethanolic extract used for the evaluation of antioxidant activity (by oxidative haemolysis and lipid peroxidation inhibition assays) and antimicrobial effects against foodborne bacteria and fungi (by serial microdilution methods) [3]. The nutritional analysis revealed high levels of carbohydrates (manly fructose and glucose) and fibre and a lipid fraction consisting mainly of polyunsaturated fatty acids. Citric and ascorbic acids were detected in high amounts, as well as the four tocopherol isoforms. The hydroethanolic berry extract showed capacity for inhibiting haemolytic oxidation and lipid peroxidation, antibacterial effects against Staphylococcus aureus and Bacillus cereus, and antifungal activity against Aspergillus and Penicillium strains. Overall, this work highlighted the nutritional value of goldenberry, which has been noted as a functional fruit with a growing presence in the Portuguese market.
PB  - Basel: MDPI
C3  - The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum
T1  - Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.
IS  - 3
VL  - 6
DO  - 10.3390/Foods2021-10949
ER  - 

@conference{
author = "Añibarro-Ortega, Mikel and Pinela, José and Petrović, Jovana and Prieto, Miguel Angel and Soković, Marina and Ferreira, Isabel and Simal-Gandara, Jesus and Barros, Lillian",
year = "2021",
abstract = "European consumers have a growing interest in new fruits and flavours, which has promoted the production and commercialisation of exotic fruits such as goldenberry (Physalis peruviana L., Fam. Solanaceae). Colombia and South Africa are currently the main producers of this fruit, but it can be cultivated in almost all the highlands of the tropics and in several parts of the subtropics, given its ability to adapt to a wide range of agroecological conditions [1]. Consumer demand for this small orange berry has also been driven by its nutritional value and health-promoting effects [2]. Although there have been different studies about this fruit, the available information is still scarce. Furthermore, the fruit quality attributes can vary depending on the agricultural practices used and the edaphoclimatic conditions of the growing sites. Therefore, this study was carried out to evaluate the nutritional composition and in vitro antioxidant and antimicrobial activity of goldenberry cultivated in the northeast region of Portugal. Goldenberry samples were lyophilised and analysed to determine their proximate composition (moisture, protein, fat, ash, dietary fibre, and carbohydrates), following official methods of food analysis [3]. Their profiles of sugars, organic acids, fatty acids, and tocopherols were characterised by standardised chromatographic methods [3]. The powdered goldenberry sample was also prepared in a hydroethanolic extract used for the evaluation of antioxidant activity (by oxidative haemolysis and lipid peroxidation inhibition assays) and antimicrobial effects against foodborne bacteria and fungi (by serial microdilution methods) [3]. The nutritional analysis revealed high levels of carbohydrates (manly fructose and glucose) and fibre and a lipid fraction consisting mainly of polyunsaturated fatty acids. Citric and ascorbic acids were detected in high amounts, as well as the four tocopherol isoforms. The hydroethanolic berry extract showed capacity for inhibiting haemolytic oxidation and lipid peroxidation, antibacterial effects against Staphylococcus aureus and Bacillus cereus, and antifungal activity against Aspergillus and Penicillium strains. Overall, this work highlighted the nutritional value of goldenberry, which has been noted as a functional fruit with a growing presence in the Portuguese market.",
publisher = "Basel: MDPI",
journal = "The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum",
title = "Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.",
number = "3",
volume = "6",
doi = "10.3390/Foods2021-10949"
}

Añibarro-Ortega, M., Pinela, J., Petrović, J., Prieto, M. A., Soković, M., Ferreira, I., Simal-Gandara, J.,& Barros, L.. (2021). Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.. in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum
Basel: MDPI., 6(3).
https://doi.org/10.3390/Foods2021-10949

Añibarro-Ortega M, Pinela J, Petrović J, Prieto MA, Soković M, Ferreira I, Simal-Gandara J, Barros L. Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.. in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum. 2021;6(3).
doi:10.3390/Foods2021-10949 .

Añibarro-Ortega, Mikel, Pinela, José, Petrović, Jovana, Prieto, Miguel Angel, Soković, Marina, Ferreira, Isabel, Simal-Gandara, Jesus, Barros, Lillian, "Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal." in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum, 6, no. 3 (2021),
https://doi.org/10.3390/Foods2021-10949 . .

TY  - JOUR
AU  - Carpena, Marija
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Pereira, Carla
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Soria-Lopez, Anton
AU  - Fraga-Corral, Maria
AU  - Simal-Gandara, Jesus
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Prieto, Miguel A.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4359
AB  - The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.
PB  - Basel: MDPI
T2  - Chemosensors
T1  - Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction
IS  - 6
VL  - 9
DO  - 10.3390/chemosensors9060132
SP  - 132
ER  - 

@article{
author = "Carpena, Marija and Caleja, Cristina and Pereira, Eliana and Pereira, Carla and Ćirić, Ana and Soković, Marina and Soria-Lopez, Anton and Fraga-Corral, Maria and Simal-Gandara, Jesus and Ferreira, Isabel C. F. R. and Barros, Lillian and Prieto, Miguel A.",
year = "2021",
abstract = "The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.",
publisher = "Basel: MDPI",
journal = "Chemosensors",
title = "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction",
number = "6",
volume = "9",
doi = "10.3390/chemosensors9060132",
pages = "132"
}

Carpena, M., Caleja, C., Pereira, E., Pereira, C., Ćirić, A., Soković, M., Soria-Lopez, A., Fraga-Corral, M., Simal-Gandara, J., Ferreira, I. C. F. R., Barros, L.,& Prieto, M. A.. (2021). Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors
Basel: MDPI., 9(6), 132.
https://doi.org/10.3390/chemosensors9060132

Carpena M, Caleja C, Pereira E, Pereira C, Ćirić A, Soković M, Soria-Lopez A, Fraga-Corral M, Simal-Gandara J, Ferreira ICFR, Barros L, Prieto MA. Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors. 2021;9(6):132.
doi:10.3390/chemosensors9060132 .

Carpena, Marija, Caleja, Cristina, Pereira, Eliana, Pereira, Carla, Ćirić, Ana, Soković, Marina, Soria-Lopez, Anton, Fraga-Corral, Maria, Simal-Gandara, Jesus, Ferreira, Isabel C. F. R., Barros, Lillian, Prieto, Miguel A., "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction" in Chemosensors, 9, no. 6 (2021):132,
https://doi.org/10.3390/chemosensors9060132 . .

TY  - JOUR
AU  - Fernandes, Filipa
AU  - Pereira, Eliana
AU  - Prieto, Miguel
AU  - Calhelha, Ricardo
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2019
UR  - http://www.mdpi.com/1420-3049/24/4/686
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3277
AB  - Heat-Assisted Extraction (HAE) was used for the optimized production of an extract rich in anthocyanin compounds from Ocimum basilicum var. purpurascens leaves. The optimization was performed using the response surface methodology employing a central composite experimental design with five-levels for each of the assessed variables. The independent variables studied were the extraction time (t, 20–120 min), temperature (T, 25–85 °C), and solvent (S, 0–100% of ethanol, v/v). Anthocyanin compounds were analysed by HPLC-DAD-ESI/MS and the extraction yields were used as response variables. Theoretical models were developed for the obtained experimental data, then the models were validated by a selected number of statistical tests, and finally, those models were used in the prediction and optimization steps. The optimal HAE conditions for the extraction of anthocyanin compounds were: t = 65.37 ± 3.62 min, T = 85.00 ± 1.17 °C and S = 62.50 ± 4.24%, and originated 114.74 ± 0.58 TA mg/g of extract. This study highlighted the red rubin basil leaves as a promising natural matrix to extract pigmented compounds, using green solvents and reduced extraction times. The extract rich in anthocyanins also showed antimicrobial and anti-proliferative properties against four human tumor cell lines, without any toxicity on a primary porcine liver cell line.
T2  - Molecules
T1  - Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. purpurascens
IS  - 4
VL  - 24
DO  - 10.3390/molecules24040686
SP  - 686
ER  - 

@article{
author = "Fernandes, Filipa and Pereira, Eliana and Prieto, Miguel and Calhelha, Ricardo and Ćirić, Ana and Soković, Marina and Simal-Gandara, Jesus and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2019",
abstract = "Heat-Assisted Extraction (HAE) was used for the optimized production of an extract rich in anthocyanin compounds from Ocimum basilicum var. purpurascens leaves. The optimization was performed using the response surface methodology employing a central composite experimental design with five-levels for each of the assessed variables. The independent variables studied were the extraction time (t, 20–120 min), temperature (T, 25–85 °C), and solvent (S, 0–100% of ethanol, v/v). Anthocyanin compounds were analysed by HPLC-DAD-ESI/MS and the extraction yields were used as response variables. Theoretical models were developed for the obtained experimental data, then the models were validated by a selected number of statistical tests, and finally, those models were used in the prediction and optimization steps. The optimal HAE conditions for the extraction of anthocyanin compounds were: t = 65.37 ± 3.62 min, T = 85.00 ± 1.17 °C and S = 62.50 ± 4.24%, and originated 114.74 ± 0.58 TA mg/g of extract. This study highlighted the red rubin basil leaves as a promising natural matrix to extract pigmented compounds, using green solvents and reduced extraction times. The extract rich in anthocyanins also showed antimicrobial and anti-proliferative properties against four human tumor cell lines, without any toxicity on a primary porcine liver cell line.",
journal = "Molecules",
title = "Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. purpurascens",
number = "4",
volume = "24",
doi = "10.3390/molecules24040686",
pages = "686"
}

Fernandes, F., Pereira, E., Prieto, M., Calhelha, R., Ćirić, A., Soković, M., Simal-Gandara, J., Barros, L.,& Ferreira, I. C. F. R.. (2019). Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. purpurascens. in Molecules, 24(4), 686.
https://doi.org/10.3390/molecules24040686

Fernandes F, Pereira E, Prieto M, Calhelha R, Ćirić A, Soković M, Simal-Gandara J, Barros L, Ferreira ICFR. Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. purpurascens. in Molecules. 2019;24(4):686.
doi:10.3390/molecules24040686 .

Fernandes, Filipa, Pereira, Eliana, Prieto, Miguel, Calhelha, Ricardo, Ćirić, Ana, Soković, Marina, Simal-Gandara, Jesus, Barros, Lillian, Ferreira, Isabel C. F. R., "Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. purpurascens" in Molecules, 24, no. 4 (2019):686,
https://doi.org/10.3390/molecules24040686 . .