TY  - JOUR
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5254
AB  - Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.
PB  - Amsterdam : Elsevier
T2  - Industrial Crops and Products
T1  - Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level
VL  - 186
DO  - 10.1016/j.indcrop.2022.115146
SP  - 115146
ER  - 

@article{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.",
publisher = "Amsterdam : Elsevier",
journal = "Industrial Crops and Products",
title = "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level",
volume = "186",
doi = "10.1016/j.indcrop.2022.115146",
pages = "115146"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products
Amsterdam : Elsevier., 186, 115146.
https://doi.org/10.1016/j.indcrop.2022.115146

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products. 2022;186:115146.
doi:10.1016/j.indcrop.2022.115146 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level" in Industrial Crops and Products, 186 (2022):115146,
https://doi.org/10.1016/j.indcrop.2022.115146 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Miladinović, Marija
AU  - Gašić, Uroš
AU  - Dimitrijević-Branković, Suzana
AU  - Rajilić-Stojanović, Mirjana
PY  - 2022
UR  - https://link.springer.com/10.1007/s13399-022-02717-5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4967
AB  - Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.
T2  - Biomass Conversion and Biorefinery
T1  - Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction
DO  - 10.1007/s13399-022-02717-5
ER  - 

@article{
author = "Milutinović, Milica and Miladinović, Marija and Gašić, Uroš and Dimitrijević-Branković, Suzana and Rajilić-Stojanović, Mirjana",
year = "2022",
abstract = "Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.",
journal = "Biomass Conversion and Biorefinery",
title = "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction",
doi = "10.1007/s13399-022-02717-5"
}

Milutinović, M., Miladinović, M., Gašić, U., Dimitrijević-Branković, S.,& Rajilić-Stojanović, M.. (2022). Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery.
https://doi.org/10.1007/s13399-022-02717-5

Milutinović M, Miladinović M, Gašić U, Dimitrijević-Branković S, Rajilić-Stojanović M. Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery. 2022;.
doi:10.1007/s13399-022-02717-5 .

Milutinović, Milica, Miladinović, Marija, Gašić, Uroš, Dimitrijević-Branković, Suzana, Rajilić-Stojanović, Mirjana, "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction" in Biomass Conversion and Biorefinery (2022),
https://doi.org/10.1007/s13399-022-02717-5 . .